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BIOFILMSRESEARCH CENTER FOR BIOINTERFACES

AnnuAl RepoRt 2013

MALMÖ UNIVERSITYBIOFILMS – RESEARCH CENTER FOR BIOINTERFACES

Biofilms – Research Center for Biointerfaces

Annual Report 2013

Biofilms – Research Center for Bio inter faces

is a translational re search center covering

four research groups within three faculties

at Malmö University. The core strengths

of the Center are our broad expertise,

spanning the whole range from theoretical

modelling to clinical sciences, and our long

experience in working with industry and

relating to their needs. The general aim

of research activities at the Center is to

understand, predict and control material/

cell/tissue interactions with medical, dental,

food and environmental applications.

Biofilms – Research Center for Bio inter faces

aims to become a regional site for a creative

biomedical technology environment in the

Öresund region, facilitating collaboration

between academic research, higher educa-

tion and industry, based on pharmaceutical

technology, biotechnology and medical

technology. Together, they form a key area

that offers significant commercial growth

potential. We are striving to become a

prime entry point for regional industry

seeking translational academic expertise

in the biomedical technology field.

CENTER MISSION STATEMENT

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Annual Report 2013

AbbReviAtions

CONTENTS

Mah: Malmö University; LU: Lund University; LTH: Faculty of Engineering, Lund

University, KTH: The Royal Institute of Technology; LuTH: Luleå University of

Technology; UU: Uppsala University; LiU: Linköping University; KI: Karolinska

Institute; GU: Gothenburg University; OD: Faculty of Odontology; HS: Faculty of

Health and Society; TS: Faculty of Technology and Society. KKs: The Knowledge

Foundation; VR: The Swedish Research Council; EU: European Union; FP7: 7th

Framework Programme; SI: Swedish Institute; PI: Principal Investigator; BRCB:


DIRECTOR’S REpORT 4

NEwS 2013 5

LIST OF CENTER MEMBERS 8

RESEARCH HIgHLIgHTS 13

LIST OF RESEARCH ACTIvITIES 26

SCIENTIFIC INSTRuMENTATION AND EquIpMENT

30

COLLABORATIvE pARTNERS OF THE CENTER

37

wORkSHOpS AND CONFERENCES ORgANISED By THE CENTER

42

SEMINARS AT THE CENTER 44

pHD THESES SupERvISED By CENTER MEMBERS

46

LIST OF puBLICATIONS AT THE CENTER FROM 2013

49

CONTRIBuTIONS TO AND pARTICIpATION IN CONFERENCES AND wORkSHOpS

58

front illustration: Image of

dehydrated human unpurified

saliva in air obtained using

polarized light microsopy (yana

Znamenskaya, phD Thesis “Effect

of Hydration on Thermodynamic,

Rheological and Structural

properties of Mucin” 2013)

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Annual Report 2013

DIRECTOR’S REpORT

In October, I returned as Director of the Center after a year’s break, during which I was project manager for the Malmö University strategic platform. I would now like to take the opportunity to reflect on the past year on behalf of myself and my predecessor Johan Drott, who has moved on to new challenges. 2013 has been a busy year for the Center and operations have developed positively on many fronts.

On the staff side, 2013 has been an exciting year. Our newly recruited Professor Börje Sellergren has moved his entire operation to Malmö University and he has now settled in with his research group. Liselott Lindh became a full professor at the Department of Prosthetic Dentistry, Sergey Shleev became a full profes-sor at the Department of Biomedical Science and Krister Thuresson became asso ciate professor at the Department of Biomedical Science. Alma Mašić, Sebastian Björklund and Yana Znamenskaya all successfully defended their PhD theses. We are especially proud that Alma Mašić’s thesis “Investigation of a Biofilm Reactor Model with Suspended Biomass” was awarded a prize at Malmö University’s Annual Ceremony. The Center has also been privileged to have Cristina Glad as Chair of the Steering Committee. Cristina Glad was appointed Chair by the Pro Vice-Chancellor in January 2013 and, together with the other members of the Steering Committee, she has made a very positive and constructive contri-bution to the Center’s strategic development.

As regards the strategic development of the Center, I would like to mention that during 2013 Malmö University signed an agreement for the new laboratory facilities adjacent to Medeon in Malmö. This will give the Center an excellent basis for realising the vision

of becoming a regional site for a creative environment for biomedical technology, based on pharma ceutical technology, biotech nology and medical technology, and facilitating collaboration between academic research, higher education and industry.

During 2013, we had a portfolio of 35 research projects and collaborated with 30 dif-ferent companies. We successfully established new research collaborations with both local and international industrial companies. We produced around 90 scientific publications in peer-reviewed scientific journals and research-ers at the Center actively contributed to the national and international research com-munities through involvement in organising international meetings, including the 8th International Conference on PBL in Dentistry, The International HILIC Day and the 9th Annual Workshop of Biofilms – Research Center for Biointerfaces. Other contributions to the research community have been made through membership of editorial and scien-tific advisory boards and as guest editors for highly reputed international journals, such as ‘International Journal of Prosthodontics and Restorative Dentistry’, ‘American Journal of Dentistry, ‘ScienceJet’, ‘Non-Linear Analysis: Modelling and Control’, and ‘Colloids and Surfaces: A’.

Finally, I would like to thank all those with whom we have worked for their invaluable contribution during the past year and Malmö University for their consistent support, as well as the Knowledge Foundation, the Swedish Research Council, the Borrow Foundation, the Swedish Laryng Foundation, the European Commission, and the Swedish Institute who funded our research.

Anna Holmberg, Director Biofilms

– Research Center for Biointerfaces

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Annual Report 2013

NEwS 2013

Liselott Lindh derives her energy from her contact with dental students and patient treatments in the clinic. She graduated as a dentist in 1981, became certified specialist in Prosthetic Dentistry 1992 and defended her thesis in 2002. Liselott is now appointed as Professor of Prosthetic Dentistry at the Faculty of Odontology.

“There was never any other alternative for me than to become a dentist. It was a profession that combined my interest in crafts and to help people both in terms of oral health and quality of life,” she says.

Liselott Lindh’s research interests started from the complaints the patients were telling her, and her curiosity was the driving force to try to find an explanation to what could be the reason. Her research profile is oral surface phenomena with the aim to improve oral health and includes investigations regarding:

• saliva, blood and saliva molecular surface activity on different dental materials, and salivary pellicle composition and barrier functions in healthy, erosion and dry mouth patients,

• surface modification of dental materials for improving the oral health among the spare part human and vulnerable patient groups,

• development of saliva substitutes, and • clinical studies related to the aforemen-

tioned areas of interest.

“I meet many patients who have lost their teeth, for instance after radiotherapy, and who are unable to produce their own saliva. For instance, patients wearing removable dentures in combination with the lack of saliva, frequently experience soreness in the dry and fragile mucosa due to the wear from the dentures. There is still a lot to be done for these patients to increase their oral func-tion and comfort and thus their life quality.”

newly Appointed pRofessoR with A focus on oRAl suRfAce phenomenA And oRAl heAlth

1.

newly Appointed pRofessoR with A pAssion foR bioelectRonics

2.

With a burning interest in science, Sergey Shleev has never been in any doubt that research was to be his future. He became pro-fessor in 2013. Shleev has conducted research concerning biological electric power sources over a long period and his general field of interest is bioelectronics.

“We have recently accomplished a major EU project in which we produced and tested a self-contained (self-powered and wireless) device for diabetics. Using a biofuel cell, a sensor, and a transmitter, the blood sugar level can be monitored and a signal can be sent to your mobile telephone when it is time

for an insulin injection. We’re well on the way and we’re now applying for funding to proceed and improve the device, specifically to miniaturize it.”

At the same time, he and his colleagues have a discovery in the pipeline.

“We have produced a new and unique source of electrical energy; a discovery that we are seeking to patent. The energy source is eco-friendly and there is an extremely broad field of use, from road vehicles to implants. We are also considering commercialising it at some point in the future.”

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Annual Report 2013

3. KRisteR thuResson new AssociAte pRofessoR

Krister Thuresson conducts research into self-association and aggregation of surface-active substances. He is happiest working at the border between academia and industry.

“It is a matter of understanding what hap-pens in solutions and at interfaces,” he says. “A typical example is viscosity modifiers and stabilisers, which have an important role to play in applications ranging from paint formu-lations to eye operations. With fundamental knowledge you have the means to change and create smart solutions with robust properties.”

Krister Thuresson combines research with working at a company that manufactures creative materials, such as novel modelling

compounds for children. “As an associate professor I will consolidate my platform in academia. I will continue to supervise PhD students and degree projects and take part in teaching. By doing so, I would like to impart the feeling of what it is like as an academic to move into industry, and back. It all comes down to capitalising on your unique characteristics.”

Having ingenious ideas is for the most part not enough. It is also necessary for funding to be in place, that the idea fits into the company’s profile and, not least, that the prospective product will satisfy the market and all the legal requirements.

4. wAstewAteR systems in focus foR AwARd-winning thesis

Alma Mašić’s thesis “Investigation of a Biofilm Reactor Model with Suspended Biomass” was awarded the Malmö University prize for the best thesis. Her research has been heralded as a milestone in the development of math-ematical models and the work could lead to improved wastewater treatment and thus a better environment in the future.

“It perhaps sounds strange but it is possible with the aid of mathematics to study a course of events without needing to take samples and conduct experiments every time – simulations can be made instead. This makes it consider-ably easier to acquire an overview and making

analyses becomes much more accessible,” says Alma Mašić.

Alma Mašić’s thesis deals with how suspended biomass interacts with biofilms at wastewater treatment plants. She is now a postdoc at Eawag, a Water Research Institute in Zürich. Alma Mašić came to Biofilms – Research Center for Biointerfaces after studying at Stockholm University. “The biofilm project attracted me and it sounded extremely exciting. There are very few biofilm research centres in Europe.”

5. new chAiR of bRcb

Cristina Glad, former acting President of Bioinvent International, was appointed during the year to chair the steering committee for Biofilms – Research Center for Biointerfaces (BRCB).

“It will be extremely exciting. I hope to be involved in bringing research into the commu-nity,” she said prior to taking up the position.

Cristina Glad is a biochemist and took a PhD at Lund University with a thesis on an immunochemical analysis method. After a number of years in academia, she continued her career at Bioinvent. The company, at which Glad held several leading positions and during 2013 was acting President, is a listed company and develops antibody-based pharmaceuticals.

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Annual Report 2013

Researchers from BRCB began moving into the new premises in the Medeon area of Malmö at the end of the autumn.

“The premises are large and have very well-equipped laboratories. BRCB has expanded significantly in recent years and the new premises have come at the right time in the light of the changes that lie ahead,” says Anna Holmberg, Director.

The new premises will be an important research and innovation environment.

“Our aim is to create a meeting point for researchers, entrepreneurs and business own-ers,” says Anna Holmberg.

The new premises have advanced instru-ments and good analytical equipment. The researchers now have access to instruments such as LCMS, LCMSMS, GCMS and NMR. “We have not had access to this type of analytical equipment at Malmö University previously.”

“The move is important if research is to

continue to develop. With the new premises, BRCB’s potential to achieve its goal of becom-ing a regional meeting point for a creative environment for biomedical technology in the Öresund region, facilitating collabora-tion between academia, higher education and industry, based on pharmaceutical technology, biotechnology and medical technology. We are striving to become a prime entry point for regional industry seeking translational academic expertise in the biomedical technol-ogy field,” states Anna Holmberg.

The first to move in will be Professor Börje Sellergren’s research group, who are working with molecular imprinting. “There is already considerable interest among regional companies in using our equipment. Ultimately, it could be possible to share prem-ises, creating a situation where academics and industry representatives would be working side by side.”

Anna Holmberg is convinced that the new premises will benefit both BRCB’s own research projects as well as collaboration with industry.

“I have had contact with BRCB in recent years and I know a number of the researchers from previously. Many centres of this nature fail to survive when the first few years of funding

come to an end. BRCB, however, has suc-ceeded in finding a structure and has contin-ued to develop. It is extremely exciting to be involved and make a contribution,” she says.

bRcb moves to new pRemises AdvAnced lAboRAtoRies in the medeon AReA will open up new oppoRtunities.

6.

During the autumn, Malmö University gained access to new premises in the Medeon area. The first to move in are researchers at Biofilms – Research Center for Biointerfaces (BRCB). Anna Holmberg, Director of the Center, can foresee major opportunities now that the researchers have access to well-equipped laboratories.

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Annual Report 2013

LIST OF CENTER MEMBERS

1. mAnAgement And boARdssteeRing committee

Cristina Glad

Chair

CEO C glad Consulting AB

Thomas Arnebrant

Representative Faculty of Health and Society

Gunnel Svensäter

Representative Faculty of Odontology

Karl Obrant

Head of Research, Skåne university Hospital

Markus Johnsson

Senior Director, pharmaceutical Development, Camurus AB

Ulf Brogren

CEO promimic AB

Peter Nordström

Senior project Manager,

Medicon valley Alliance

senioR ReseARcheRs foRum

Johan Drott /Anna HolmbergChairphD, Director

Ann Wennerbergprofessor

Liselott Lindhprofessor

Gunnel Svensäterprofessor

Julia Daviesprofessor

Bertil KinnbyAssoc. professor

Claes WickströmAssoc. professor

Per Jönssonprofessor

Christina Bjerkén

Assoc. professor

Tautgirdas Ruzgasprofessor

Thomas Arnebrantprofessor

Vitaly Kocherbitov

Assoc. professor

Johan Engblom

Assoc. professor

Anette Gjörloff-Wingren

Assoc. professor

Maria Stollenwerk

phD

During 2013, the Center comprised the following members.

2.

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Annual Report 2013

peRmAnent stAff

Anna Holmberg

phD, Director from October, 2013

Johan Drott, PhD

Director January–May, 2013

Thomas Arnebrant

professor, Acting Director May–October, 2013

Alexei Iantchenko

professor

Ann Wennerberg

professor

Börje Sellergren

professor

Gunnel Svensäter

professor

Håkan Eriksson

professor

Julia Davies

professor

Lennart Ljunggren

professor

Liselott Lindh

professor

Per Jönsson

professor

Sergey Shleev

professor

Tautgirdas Ruzgas

professor

Anette Gjörloff-Wingren

Assoc. professor

Bertil Kinnby

Assoc. professor

Christina Bjerkén

Assoc. professor

Claes Wickström

Assoc. professor

Johan Engblom

Assoc. professor

Ryo Jimbo

Assoc. professor

Vitaly Kocherbitov

Assoc. professor

Zoltan Blum

Assoc. professor

Gabriela Enggren

phD

Jakob Blomqvist

phD

Javier Sotres

phD

Jessica Neilands

phD

Lars Ohlsson

phD

Maria Stollenwerk

phD

Tove Sandberg

phD

Yuanji Cheng

phD

3.

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Annual Report 2013

JunioR ReseARcheRs And post-docs

Alejandro Barrantes

phD

Anna Runnsjö

phD

Gesche Graf

phD

Hala Ghandour Gari

phD

Jildiz Hamit Eminovski

phD

Jonas Carlstedt

phD

Justas Barauskas

phD

Lars Söderberg

phD

technicAl And AdministRAtive stAff

Eva Nilsson

Administrative coordinator

Agnethe Henriksson

Technician

Madeleine Blomqvist

Technician

Ulrika Troedsson

phD, Technician

Peter Falkman

phD, Research engineer

4.

Maria Falck Miniotis

phD

Pär Olsson

phD

Soma Ghosh

phD

Sudhirkumar Shinde

phD

5.

phd students

Alma Mašić (enrolled at Lu)

Anton Fagerström

Cathrine Albér

Celina Wierzbicka

Deyar Mahmood

Dmitrii Pankratov

Francesca Cecchinato

Jonas Anderud

Liselott Ellmarker-Löfquist

Magnus Falk

Marija Jankunec

(enrolled at vilnius university, vilnius, Lithuania)

Mariko Hayashi

Marjan Dorkhan

Mark Galat

Michael Braian

Patrick Seumo

(enrolled at university of yaounde I, yaounde, Cameroon)

Peter Lamberg

6.

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Annual Report 2013

guests

Thomas Zemb

Institute for Separation Chemistry

of Marcoule, France

Sergei Gavryushov

Russian Academy of Sciences, Moscow,

Russia

Robert Burger

DTu Nanotech, Copenhagen

Marco Donolato

DTu Nanotech, Copenhagen

Aušra Linkevičiūtė

vilnius university, Lithuania

Galina Pankratova

Bryansk State university, Bryansk, Russia

Wolfgang Bäther

Dräger Ag, germany

Stefan Lehman

Dräger Ag, germany

Anthony Rees

Biotage AB, Sweden

Jenifer Thewalt

SFu, vancouver, Canada

Ben Boyd

Monash university, Australia

Per Claesson

The Royal Institute of Technology, Stockholm,

Sweden

Malin Sjöö

Faculty of Engineering, Lund university,

Sweden

Per Hansson

uppsala university, uppsala, Sweden

Gordon Proctor

kings College, London, uk

Nayab Chaudhury

kings College, London, uk

Mogens Kilian

Aarhus university, Denmark

Tracey Winning

university of Adelaide, Australia

7.

Pär Johansson

Ramesh Chowdhary

Ricardo Trindade

Sebastian Björklund

(enrolled at Lu)

Silvia Galli

Sing Yee Yeung

Tuerdi Maimaitiyili

(enrolled at Lu)

Ulf Hejman

(enrolled at Lu)

Vida Krikstolaityte

(enrolled at vilnius university, vilnius,

Lithuania)

Yana Znamenskaya

Zahra El-Schich

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Annual Report 2013

Charles Schuler

university of British Columbia, Canada

Henk Schmidt

Erasmus university, the Netherlands

Magnus Fornbacke

Enzymatica AB, Sweden

Mats Clarsund

Enzymatica AB, Sweden

Nigel Borrow

The Borrow Foundation, uk

Andrew Rugg-Gunn

Newcastle university, uk

Svante Twetman

Copenhagen university, Denmark

Per Rabe

Hallands Läns Landsting, Sweden

Barbera Musi

gambro AB, Lund, Sweden

Torbjörn Linden

gambro AB, Lund, Sweden

Helena Jeppson

gambro SB, Lund, Sweden

Anders Wieslander

gambro SB, Lund, Sweden

Jan Hall

Nobel Biocare, gothenburg, Sweden

Carina Norberg

public Oral Health Care Service, Blekinge,

Sweden

Christina Stebring

public Oral Health Care Service, kronoberg,

Sweden

Maria Hildning

public Oral Health Care Service, kronoberg,

Sweden

Margareta Elfvin

public Oral Health Care Service, kalmar,

Sweden

Amina Basic

university of gothenburg, Sweden

Andreas Sonesson

Lund university, Sweden

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Annual Report 2013

Biofuel cells (BFCs) produce electrical

energy by oxidizing glucose, lactate,

ethanol, etc., i.e., energy rich bioorganic

molecules present in biological tissues. To

facilitate the oxidation BFCs exploit bio-

logical catalysts, e.g., enzymes and living

cells immobilized at the electrodes. BFCs

based on three-dimensional (3D) nano-

structures loaded with enzymes represent

one of the most compact BFC designs. 3D

nanostructures are usually obtained by

modifying BFC electrodes with nanomate-

rials, such as carbon nanotubes or metallic

nanoparticles. The main advantages of

BFCs are the possibility to fuel the devices

with renewable substrates and a relatively

easy realization of implantable design.

Performance of implanted BFCs in animals

has been studied and reviewed (Falk et

al. 2013). However, demonstrating the

performance of BFCs in implanted situa-

tions rarely address their biocompatibility

issue down to cellular level.

Recently, we have conducted the very

first study of a BFC in cell culture. BFC

comprised Corynascus thermophilus

cellobiose dehydrogenase (CDH) based

bioanode and Myrothecium verrucaria

bilirubin oxidase (BOx) based biocathode.

The electrodes were constructed at the

bottom of a cell culture plate (Figure 1).

The BFC had a power density of 25 µW

cm-2 at 0.5 V potential in simple buffer

solution and in cell culturing medium.

L929 murine fibroblast cells were seeded

on top of the BFC and possible effects of

the BFC on the cells and vice versa were

studied. It was shown that on average the

power of the BFC drops by about 70%

under a nearly confluent layer of cells. The

BFC appeared to have a toxic effect on the

L929 cell line (Figure 2). It was concluded

that the bioanode, consisting of CDH,

produced hydrogen peroxide at toxic

concentrations. However, the toxic effect

was circumvented by co-immobilizing

catalase on the bioanode.

RESEARCH HIgHLIgHTS

peRfoRmAnce of enzymAtic fuel cell in cell cultuRe

Falk, M., Villarrubia, C., Babanova, S., Atanassov, P., Shleev, S. (2013) Biofuel Cells for Biomedical Applications: Colonizing the Animal Kingdom. ChemPhysChem 14(10), 2045–2058.Lamberg, P., Shleev, S., Ludwig, R., Arnebrant, T., Ruzgas, T. (2014) Performance of enzymatic fuel cell in cell culture. Biosens. Bioelectron. 55, 168–173.

contact: [email protected]

figure 2. Image of L929 cells close to the edge of the anode electrode. It can be seen that L929 cells are more round at the edge of the electrode (black) indicating the toxicity of BFC. In this study it was determined that peroxide is produced as a side reaction of CDH at the anode. The toxic effect was circumvented by co-immobilizing catalase together with CDH.

figure 1. Construction of biofuel cell (BFC) at the bottom of cell culture plate.

1.

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Annual Report 2013

Miniature, self-contained biodevices

powered by biofuel cells may enable a

new generation of implantable, wireless,

minimally invasive neural interfaces for

neurophysiological in vivo studies and

for clinical applications. We report on the

fabrication of a direct electron transfer

based glucose/oxygen enzymatic fuel cell

from genuinely three-dimensional nano-

structured microscale gold electrodes,

modified with suitable biocatalysts. We

show that the process underlying the

simple fabrication method of three-

dimensional nanostructured electrodes

is based on an electrochemically driven

transformation of physically deposited

gold nanoparticles. We experimentally

demonstrate that mediator-, cofactor-,

and membrane-less enzymatic fuel cells

do operate in cerebrospinal fluid and in

the brain of a rat, producing amounts of

electrical energy sufficient to drive a self-

contained biodevice, viz. 7 µW cm-2 in

vitro and 2 µW cm-2 in vivo at an operating

voltage of 0.4 V. Last but not least, we

also demonstrate an inductive coupling

between three-dimensional nanobioelec-

trodes and living neurons.

enzymAtic fuel cells bAsed on micRoscAle nAnostRuctuRed electRodes opeRAting in vivo

2.

Andoralov V., Falk M., Suyatin D.B., Granmo M., Sotres J., Ludwig R., Popov

V.O., Schouenborg J., Blum Z., and Shleev S. (2013) Biofuel cell based on

microscale nano structured electrodes with inductive coupling to rat brain neurons.

Scientific Reports 3, 3270.

contact : [email protected]

Enzymatic fuel cell implanted into the rat brain (according to Andoralov et al., 2013). Close up pictures of the implantation with (left) and without (right) localised surface bleeding.

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Annual Report 2013

Two blue multicopper oxidases (MCOs),

viz. Trametes hirsuta laccase (Lc) and

Myrothecium verrucaria bilirubin oxidase

(BOx), were immobilised on bare polycrys-

talline gold (Au) surfaces by direct adsorp-

tion from both dilute and concentrated

enzyme solutions. The adsorption was

studied in situ by means of null ellipso-

metry. Moreover, both enzyme modified

and bare Au electrodes were investigated

in detail by atomic force microscopy

(AFM), as well as electrochemically. When

adsorbed from dilute solutions (0.125 and

0.25 mg mL-1 in the case of Lc and BOx,

respectively) the amount of enzyme per

unit area was determined to be ca. 1.7

and 4.8 pmol cm-2, whereas the protein

film thickness was determined to be 29

and 30 Å for Lc and BOx, respectively. A

well-pronounced bioelectrocatalytic reduc-

tion of molecular oxygen was observed on

BOx/Au biocathodes, whereas this was not

the case for Lc modified Au electrodes, i.e.

adsorbed Lc was catalytically inactive.

The initially observed apparent catalytic

constants for the adsorbed BOx and the

enzyme in solution were found to be very

close to each other, viz. 54 s-1 and 58 s-1,

respectively (pH 7.4, 25ºC). However, after

3 h of operation of BOx/Au biocathodes

the apparent catalytic constant dropped

to 23 s-1. Based on experimental results,

conformational changes of the enzymes

(in all likelihood, their flattening on the Au

surface) were suggested to explain deactiva-

tion of MCOs on the bare Au electrodes.

Pankratov D., Sotres J., Barrantes A.,Arnebrant T., Shleev S. (2014) Interfacialbehaviour and activity of laccase andbilirubin oxidase on bare gold surfaces.Langmuir 30, 2943-2951.

contact: [email protected]

inteRfAciAl behAviouR And Activity of blue multicoppeR oxidAses on bARe gold suRfAces

3.

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Annual Report 2013

Biofilm systems always have a certain

amount of suspended biomass present in

the reactor, mostly due to detachment of

biomass from the biofilm. The amount of

suspended biomass is generally smaller

than in a proper activated sludge reac-

tor, but may still influence the growth of

the biofilm as it is retained in the reactor

long enough for reattachment to occur.

Traditional mathematical biofilm models

typically do not include suspended bio-

mass even when the detachment process

is included, thereby assuming that any

effects due to suspended biomass can be

neglected. While this assumption can be

reasonable for certain lab-scale reactors, it

may be of critical importance for biofilm

reactors.

A mathematical and numerical analysis

of a dynamic one-dimensional model of

biofilm and suspended biomass in a con-

tinuous stirred tank reactor for a single

species single substrate setting, including

attachment and detachment, showed that

bacteria will either be completely washed

out or coexist as biofilms and suspended

biomass. Furthermore, suspended biomass

was found to be relatively more efficient

at substrate removal than biofilms, even

though the main contribution to removal

came from the biofilm, see Figure 1.

Masic, A. (2013) Investigation of a biofilm reactor model with suspended biomass.

PhD thesis, Lund University, Sweden.

Masic, A., Eberl, H.J. (2014) A modeling and simulation study of the role of

suspended microbial populations in nitrification in a biofilm reactor,

Bull. Math. Biol. 76(1):27-58.

Masic, A., Eberl, H.J. (2014) On optimization of substrate removal in a

bioreactor with wall attached and suspended bacteria, Math. Biosci. Eng. (to appear).


investigAtion of A biofilm ReActoR model with suspended biomAss

4.

With an extended model for a consider-

ably more complex multi-species multi-

substrate nitrifying mobbing bed biofilm

reactor in a wastewater setting, it was

revealed through a computational study

that the incorporation of suspended bio-

mass is significant for detailed descriptions

of the internal processes and intermediate

steps of nitrification. However, suspended

biomass did not affect the overall reactor

performance and need not be considered

if such results are the sole objective. In

the above studies it was assumed that the

required amount of untreated wastewater

is always available, which is certainly not

always a correct assumption. Moreover,

some situations require only a certain

amount of wastewater to be treated as

quickly as possible. Therefore, an optimal

control problem was defined, where the

control was selected as the flow between a

storage tank, holding the untreated waste-

water, and a biological treatment reactor.

figure 1. percentage of substrate removal performed by suspended biomass (left) and amount of suspended biomass relative to total biomass (right), at steady state as a function of biofilm area for different dilution rates D.

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Annual Report 2013

The problem was constructed with two

objectives: to remove as much substrate as

possible and to treat the water as quickly

as possible. It was found that the potential

for reactor improvement by varying the

flow rate is relatively modest and mostly

due to an initial transient phase during

which the bacteria adjust to their sur-

roundings. Since such initial conditions

in a reactor are usually unknown, the

implementation of such a control would

also be impractical.

Reduced AdheRence of oRAl stReptococci but not soft-tissue cells to AnodicAlly-oxidized titAnium Abutment suRfAces

5.

Abutments of endosseous dental implants

protruding through the mucosa, are

exposed to microorganisms in saliva.

Early colonizers initiate biofilm formation

and promote binding of later colonizers,

leading to the development of complex

plaque. Inflammatory responses to plaque

biofilms can lead to breakdown of sup-

porting bone tissue and implant failure.

A cuff of keratinized mucosa adhering to

an abutment surface provides a barrier

to the passage of microorganisms into

the peri-implant connective tissues and

is thus regarded as a key factor for the

long-term success of implant therapy. We

have therefore investigated both biofilm

formation and soft-tissue cell adherence to

anodically-oxidized (AOX1 and AOX2)

titanium surfaces designed for use in

implant abutments.

Scanning electron microscopy showed a

nanoporous structure on the anodically-

oxidized surfaces and near-edge X-ray

absorption revealed a higher anatase

content than commerically pure titanium

(CpTi) (Fig 1). Human oral keratinocytes

and gingival fibroblasts both adhered well,

with no significant differences between the

anodically-oxidized and CpTi surfaces (Fig

2). The adhesion strength of keratinocytes

was greater than that of fibroblasts but

no differences between the anodically-

oxidized and the CpTi surfaces were seen

for either cell type.figure 1. NEXAFS spectra of CpTi control (black line) and anodically-oxidized (green and red lines) and the two crystal phases of titanium oxide; anatase and rutile.

figure 2. Adherence of human gingival fibroblasts and oral keratinocytes to CpTi, AOX1 and AOX2 surfaces. Adhered cells were stained with Live/Dead BacLight. Scale bars represent 50µm.

18


Annual Report 2013

Dorkhan M, Yucel-LindbergT, Hall J, Svensäter G, Davies JR. Adherence of

human oral keratinocytes and gingival fibroblasts to nanoporous titanium

surfaces. BMC Oral health (submitted).

Dorkhan M, Hall J, Uvdal P, Sandell A, Svensäter G, Davies JR. Adherence of oral

streptococci to crystalline anatase-rich titanium. Biofouling (submitted).


Four early colonizing oral streptococci

(Streptococcus gordonii, Streptococcus

mitis, Streptococcus oralis and Streptococcus

sanguinis) showed significantly reduced

binding to the anodically-oxidized sur-

faces than to the CpTi control (Fig 3). In

ad dition, exposure to the bacteria, reduced

adherence of keratinocytes to all surfaces

as well as causing damage to the cells.

Thus anatase-rich, nanoporous titanium

surfaces prepared by anodic oxidation

appear to hold promise for use in dental

implant abutments since they can reduce

binding of oral streptococci while at

the same time allowing fibroblasts and

keratinocytes to attach to the surface.

figure 3. graphs showing mean coverage values ± SEM from three independent experiments expressed as % of coverage on the control surfaces, analyzed using students t- test (*p<0.05, **p<0.01 and ***p<0.001).

19


Annual Report 2013

Periodontitis is initiated by increased flow

of protein-rich gingival crevicular fluid

resulting from the inflammatory response

of the host to biofilm accumulation. This

ecological change favours bacteria with a

proteolytic phenotype. The bacterial pro-

teases and breakdown products contribute

to, and trigger, the inflammatory reaction

resulting in tissue breakdown.

The serpin plasminogen activator inhibitor

type 2 (PAI-2/Serpin B2) is an inhibitor

of serine proteases and has been detected

in very high concentrations in gingival

fluid. The aim of the present study was

thus to investigate possible interactions

between PAI-2 and proteases produced by

a multispecies bacterial consortium with

a composition reflecting conditions in a

periodontal pocket.

A multispecies bacterial consortium com-

prising ten strains was grown for seven

days. PAI-2 was added to the biofilm con-

sortium and incubated for 1 hour followed

by the addition of FITC-labeled casein or

a serine protease substrate (H-Gly-Pro-

AMC). After the incubation period the

fluorescence in the supernatant was meas-

ured and the biofilm cells were examined

in a confocal scanning laser microscope.

PAI-2 showed a concentration-dependent

inhibition of bacterial proteolytic activity.

To our knowledge this is the first time that

PAI-2 has been shown to inhibit bacterial

proteases. Given the high concentration

of PAI-2 in the gingival region, this might

indicate that PAI-2 plays a role for the

integrity of the epithelial barrier.

seRine pRoteAse inhibitoR pAi-2 inhibits bActeRiAl pRoteAses

6.

Neilands J, Svensäter G, Kinnby B. Serine protease inhibitor PAI-2 inhibits bacterial proteases. Manuscript


figure 2. proteolytic activity in ten species biofilm consortium in the presence or absence of low/high molecular (LMw/HMw) weight pAI-2 showing approximately 50% inhibition compare to control.

figure 1 . Ten species biofilm consortium incubated with serine protease substrate (H-gly-pro-AMC) after incubation with/without pAI-2. Bacteria with proteolytic activity appear blue.

20


Annual Report 2013

We have shown that L. fermentum adheres

well to surfaces coated with MUC5B

mucin. In biofilms of L. fermentum formed

in a MUC5B environment, the proportion

of protease active cells (47 ± 0.6% of

the population) was significantly greater

(p<0.01) than that in biofilms formed

in nutrient broth (0.4 ± 0.04% of the

population) (Fig 1). Comparison of the

surface proteomes of biofilm cells of L.

fermentum in a MUC5B environment with

those in nutrient broth using two-dimen-

sional electrophoresis (2DE) and mass

spectroscopy, showed that the enhanced

proteolytic activity was associated with

increased expression of a glycoprotease;

O-sialoglycoprotein endopeptidase, but

surprisingly the most significant changes in

expression were seen in chaperone proteins

such as DnaK and trigger factor.

We conclude that adhesion to mucin-

coated surfaces leads to a shift towards a

suRfAce-AssociAted chApeRones (tRiggeR fActoR And dnAK) ARe pivotAl foR incReAsed pRoteAse Activity in lActobAcillus feRmentum biofilms on humAn sAlivARy muc5b mucin

7.

Wickström C, Chávez de Paz L, Davies JR, Svensäter G. Surface-associated

MUC5B mucins promote protease activity in Lactobacillus fermentum biofilms

(2013) BMC Oral Health 13(1):43. DOI: 10.1186/1472-6831-13-43.


Mucosal surfaces are coated with layers

of mucus gel that protect the underly-

ing tissues and promote colonization by

members of the commensal microflora.

Lactobacillus fermentum is a common

inhabitant of the oral cavity, gastrointes-

tinal and reproductive tracts and is one

of the most important lactic acid bacteria

contributing to the formation of a healthy

intestinal microflora.

more protease-active phenotype within L.

fermentum biofilms and that the enhanced

proteolytic activity was associated with an

increase in O-sialoglycoprotein endopepti-

dase on the cell surface. Upregulation of

chaperone proteins in the mucin environ-

ment may contribute to the proteolytic

phenotype through activation of the

glycopeptidase. This would represent one

way for commensal lactobacilli e.g. L. fer-

mentum to exploit complex substrates in

their local environment in order to survive

on mucosal surfaces.

figure 1. proteolytic activities of Lactobacillus fermentum biofilm populations. CSLM images showing biofilm cells growing in (a) a MuC5B environment or (b) a nutrient broth environment. proteolytic activity was visualized by incubation with a FITC-conjugated casein substrate (green) for 1 hour. Cells were counterstained with Syto 24 (red).

figure 2. 2DE of surface proteins from L. fermentum. proteins isolated from bacteria in a MuC5B-environment (left) or a nutrient broth environment (right) were subjected to IEF in a pH 4–7 gradient followed by SDS-pAgE in 14% gels. gels were silver-stained and image analysis of differently expressed proteins performed using Delta 2D software.

21


Annual Report 2013

Caries and periodontitis are biofilm-

mediated oral diseases, resulting from

complex interactions between the com-

mensal microbiota, host susceptibility and

environmental factors. Bacteria growing

in biofilms are often rather resistant to

antimicrobial substances and studies on

biofilm formation and architecture in

vivo are therefore important in order to

investigate the effect of antibacterial treat-

ments. However, harvesting undisturbed

plaque samples from teeth for viewing is

a practical challenge.

Here, we describe a novel method to study

dental plaque formation and the effects of

antibacterial measures in vivo. Discs of

human enamel were fixed to abutments

at two premolar implant sites in three

healthy volunteers. A biofilm was allowed

to develop for 7 days, after which one

control disc was removed. The patients

then rinsed with either sodium fluoride

or chlorhexidine and the treated disc was

also removed. The samples were analyzed

using an inverted confocal laser scanning

microscope (Nikon TE2000). Post pro-

cessing and image analysis was performed

with ImageTrak software (http://www.

ucalgary.ca/styslab/imagetrak).

The three subjects accumulated different

amounts of visible plaque over the test

period (Figure 1).

A novel method to hARvest undistuRbed oRAl biofilms foR confocAl lAseR scAnning micRoscope

8.

Rabe P, Twetman S, Kinnby B, Davies J, Svensäter G. A novel method to harvest undisturbed oral biofilms for confocal laser scanning microscopy. Manuscript.


Bacterial vitality was high throughout

the control biofilms, with the highest

proportion of viable cells closest to the

enamel surface. Chlorhexidine exhibited

the greatest effect upon bacteria in the

upper layers of the biofilm, while fluoride

appeared to penetrate and exert an effect

in the deeper levels of the biofilm (Figure

2) No obvious effects upon biofilm thick-

ness were seen suggesting that the agents

did not remove the biofilms.

figure 2. Sagittal profiles showing the appearance of biofilms after rinsing with chlorhexidine and sodium fluoride.

figure 1. Different appearance of the control biofilms from three different subjects.

22


Annual Report 2013

Implant surface properties have long

been identified as an important factor to

promote osseointegration. The importance

of nanostructures and hydrophilicity on

osseointegration has gained much interest

in the last years. The aim of this study

was to investigate how nanostructures

and wettability influence osseointegration

and to identify whether the wettability, the

nanostructure or both in combination play

the key role in improved osseointegration.

study layout: Twenty-six adult rab-

bits each received two Ti grade 4 discs in

each tibia. Four different types of surface

modifications with different wettability

and nanostructures were prepared: hydro-

phobic without nanostructures (SLA), with

nanostructures (SLAnano); hydrophilic

with two different nanostructure densi-

ties (low density: pmodSLA, high density:

SLActive). All four groups were intended

to have similar chemistry and microrough-

ness. The surfaces were evaluated with

contact angle measurements, X-ray pho-

toelectron spectroscopy, scanning electron

microscopy, atomic force microscopy and

interferometry. After 4 and 8 weeks healing

time, pull-out tests were performed.

results: SLA and SLAnano were hydro-

phobic, whereas SLActive and pmodSLA

were super-hydrophilic. No nanostruc-

tures were present on the SLA surface,

but the three other surface modifications

clearly showed the presence of nanostruc-

tures, although more sparsely distributed

on pmodSLA. The hydrophobic samples

showed higher carbon contamination

levels compared with the hydrophilic

samples. After 4 weeks healing time,

SLActive implants showed the highest

pull-out values, with significantly higher

pull-out force than SLA and SLAnano.

After 8 weeks, the SLActive implants had

the highest pull-out force, significantly

higher than SLAnano and SLA.

conclusions : The strongest bone

response was achieved with a combina-

tion of wettability and the presence of

nanostructures (SLActive).

nAnostRuctuRes And hydRophilicity influence osseointegRAtion: A biomechAnicAl study in the RAbbit tibiA

9.

Nanostructures and hydrophilicity influence osseointegration: a

biomechanical study in the rabbit tibia. Wennerberg A, Jimbo R, Stübinger

S, Obrecht M, Dard M, Berner S. Clin Oral Implants Res. 2013

A head of print Jun 19


figure 1. The prepared implant site in the rabbit tibia (left). The discs are covered with poly-tetrafluoroethylene caps and secured by a titanium band (right).

figure 2. Fixation of the bone during the tensile testing.

23


Annual Report 2013

Living organisms have developed (bio)

minerals with properties which at present

surpass those of their synthetic counter-

parts. There is still poor knowledge on

the mechanisms by which living organ-

isms control the growth and structure of

biominerals. Although there is an agree-

ment in that phosphorylated biomolecules

play a major role in regulating these

processes, it is still not clear whether this

regulation is exerted by molecules pre-

sent in the bulk or by those adsorbed on

biomineral surfaces and, in the latter case,

the nature of the molecule-mineral surface

interactions. To approach this problem,

we have investigated the interaction

between two model phosphoproteins (κ-

and β-casein) and hydroxyapatite (HAP),

i.e. the main building block in vertebrate

bones and teeth. Characterization of these

systems by means of ellipsometry and

QCM-D revealed that both phosphopro-

teins adsorb on HAP forming homogene-

ous elastic highly hydrated monolayers.

The mechanical stability of these films

was studied at the nanoscale by means

of an atomic force microscope operated

in the friction force spectroscopy mode.

This methodology provides the strength

of the adsorbed films in terms of the

forces needed for breaking and removing

them. The forces measured for β-casein

films were higher than those measured

for κ-casein by approximately a factor

four. Considering that β-casein possesses

five phosphorylated (SerP) residues and

κ-casein one, our data implies that both

caseins bind to the HAP surfaces through

their SerP sites and that the strength

of the films is critically dependent on

the SerP-Ca interaction. These results

may shed light e.g. on the mechanisms

by which phosphoproteins affect HAP

formation/dissolution.

on the oRigin of hydRoxyApAtite stAbilizAtion by phosphoRylAted biomolecules

10.

Sotres J., Barrantes A., Lindh L., Arnebrant T. 2014. Strategies for a direct characterization of phosphoproteins on hydroxyapatite surfaces. Caries Res. 48: 98-110.


figure. Load vs Friction vs Roughness plots corresponding to the AFM-based scratching of β- and κ–casein films on HAp surfaces. Rupture and Removal events are indicated in both plots. AFM topographies, which show the scratched areas, are included in the upper-right corners of the plots.

24


Annual Report 2013

Sorption calorimetry is a very efficient

method to study hydration of biomol-

ecules and nanomaterials and it is used

in our laboratory in many projects. It has

although some limitations, in particular it

requires a high sample mass. Quartz crystal

microbalance with dissipation monitoring

(QCM-D) requires much lower sample

mass, but in hydration studies it typically

has a very low resolution since only a lim-

ited number of saturated salt solutions is

used. We introduced a new method for con-

tinuous measurements hydration processes

using QCM-D, where a non-saturated

solution of lithium chloride is used to set

up humidity of the air in contact with the

sample. During the experiment, the salt

solution is continuously diluted and the

relative humidity is continuously scanned

from about 11% up to almost 100%.

This technique was used to determine

the water sorption isotherm of lysozyme

films deposited on silica sensors as well

as to study rheological properties that

change during hydration-induced transi-

tions of the films. The sorption isotherm

obtained in QCM-D experiments is in

good agreement with sorption calorimetry

data. Analysis of the rheological behavior

during hydration of lysozyme films

revealed the presence of two transitions

at the water activities 0.67 and 0.91,

which are connected to the glass transi-

tion. Combination of the QCM-D data

and sorption calorimetric data allows

deeper understanding of glass transitions

in proteins. The new humidity scanning

QCM-D technique can be used for studies

of biomolecules available in tiny amounts.

development of humidity scAnning Qcm-d method

11.

Graf, G.; Kocherbitov, V. Determination of Sorption Isotherm and Rheological Properties of Lysozyme Using a High-

Resolution Humidity Scanning QCM-D Technique. J.Phys.Chem.B. (2013), 117,

10017−10026.


figure. The experimental setup (left) and the results obtained on hydration of lysozyme (below)

25


Annual Report 2013

Over the past two decades, technolo-

gies such as chromatography and mass

spectrometry have driven the biochemi-

cal analyses of complex lipid mixtures,

tremendously advancing our knowledge of

lipid diversity. Lipid extraction (preceding

analysis), however, is still largely based on

partitioning procedures developed in the

1950s. Although appropriate for many

abundant components, these approaches

result in variable recovery of the less-

abundant and highly charged lipids,

including phosphorylated signaling lipids

whereof most biologically active lipids

contain at least one phospho-monoester,

for example, sphingosine-1-phosphate

(S1P). Long chain amino alcohols, gener-

ally referred to as long chain bases (LCB)

and their phosphorylated forms (LCB-P),

display particularly diverse chemistries

across biological species and tissues. In

human plasma, d18:1 sphingosine is the

most abundant LCB-P. However little is

known about the presence of other LCB-Ps

with different chemical and structural

composition despite the presence of vari-

ous non-phosphorylated LCB precursors.

In a collaboration led by National

University of Singapore, we have charac-

terized and quantified 10 LCB-P species

which were not described previously as

well as 6 LCB-P not known to be present

in the given biological samples tested here,

roughly doubling the number of known

LCB-P. This work has immediate relevance

for our understanding of S1P biology,

its therapeutic targeting and the LCB-P

signaling machinery which likely depends

on the precise chemical nature of their

aliphatic portion. The flow of sphingolipid

intermediates into sphingolipids (SPL) and

glycerophospholipids (GPL)/other lipids is

also of substantial general interest.

Thus, ‘deep profiling’ is a promising new

avenue in biochemical lipidomics for

the discovery of new bioactive lipids.

Crucial in this context is the integration

of synthetic capture phases developed

in Malmö designed to excert molecular

recognition of specific lipid motifs. Our

improved method is therefore expected

to have a pronounced impact in a variety

of fields.

‘deep pRofiling’ using enhAnced AnAlyticAl woRKflows ReveAls new sphingoid bAse-phosphAte species

12.

P. Narayanaswamy, S. Shinde, R. Sulc, R. Kraut, G. Staples, C. Thiam, R. Grimm, Börje Sellergren, F. Torta and M. R. Wenk; “Lipidomic “Deep profiling”: An enhanced workflow reveal new molecular species of signaling lipids” Anal. Chem. 2014.(Article ASAP) (DOI: 10.1021/ac4039652)


26


Annual Report 2013

LIST OF RESEARCH ACTIVITIESThe research activities of the Center during 2013 are presented under the headlines below.

In addition Center members are partners

in the eu fp7 Marie Curie Initial Training

Network “pepmip- Robust affinity mate-

rials for applications in proteomics and

diagnostics” (with Börje Sellergren as

Coordinator), eu fp7 Marie Curie Initial

Training Network “MagnIM” (Ann

Wennerberg), the eu fp7 Marie Curie

Initial Training Networks “Biofuel cells-

from fundamentals to applications in bio-

electrochemistry” (Sergey Shleev) and the

eu cooperation and mobility programme

Erasmus Mundus ember (Tautgirdas

Ruzgas).

27


Annual Report 2013

cell-suRfAce inteRActions

Mechanism of Toxicity of Aluminium-

based Adjuvant (ABA) Nanomaterials

Funded by the British Medical Research

Council, MRC

pI: Håkan Eriksson

Analysis of the Cellular and Molecular

Mechanisms of Inflammatory Cells

and Cancer Cells by Using Digital

Holography and Protein Technology

PhD project funded by Mah,

Project partner: Phase Holographic

Imaging AB

pI: Anette gjörloff-wingren

Innovative Microscopy and Digital

Holography for Studies of Biomarkers

of Eukaryotic Cells Integrated to

Biosensors and Monitoring Devices

Post-doc project funded by BRCB

Project partner: Phase Holographic

Imaging AB

pI: Anette gjörloff-wingren

Biological Responses Nano-Size

Structures

Funded by the Knowledge Foundation

Project partner: Promimic

pI: Ann wennerberg

Hydrophilic and Hydrophobic Implant

Surfaces

Funded by industry

pI: Ann wennerberg

Advanced Surface Characterization


Project partner: Halmstad University

and Ospol AB

pI: Ann wennerberg

Advanced Surface Characterisation of

New Functional Biomaterials

Funded by VR

Project partners: Chalmers and

Dentsply

pI: Ann wennerberg

1.

moleculAR tRAnspoRt phenomenA

Adjuvants for Products Used in

Agriculture

Project partners: AkzoNobel Surface

Chemistry AB

Funded by the Knowledge Foundation,

Mah

pI: Johan Engblom

Water – A Crucial Factor in Regulating

Biomembrane Permeability

Project partners: Physical Chemistry, LU

Funded by FLÄK, LU

pI: Emma Sparr (Lu), Johan Engblom

Humectants and Their Mechanisms

in Skin


Project partners: SP AB, ACO Hud

Nordic AB, Eviderm Institute AB

pI: Johan Engblom

Mucoadhesion – A Prerequisite or a

Constraint in Nasal Drug Delivery


Project partners: Nares AB, Bioglan AB,

LTH

pI: Johan Engblom

2.

28


Annual Report 2013

3. moleculAR inteRActions At biointeRfAces

Hydration of Mucous Gel

Funded by Mah

pI: vitaly kocherbitov

Development of Novel Multi-Functional

Salivary Substitutes for Dry Mouth

Syndrome Patients

Funded by the Swedish Laryng

Foundation, Camurus AB, Mah

pI: Liselott Lindh

Screening of Different Surface Coatings

on Titanium including Different API’s

for Enhanced Wound Healing


Foundation and Mah

Project partner: NIOM, Oslo

pI: Liselott Lindh

Surface Modification with Natural

as well as Artificial Active Substances

of Dental Materials with the Aim to

Promote and Keep Good Oral Health

for the Spare Part Human


Foundation and Mah

Project partner: NIOM, Oslo

pI: Liselott Lindh

The Composition of Salivary Films

Formed on Surfaces with Different

Surface Wettability Including

Identification of Pellicle Proteins


Foundation and Mah

pI: Liselott Lindh

Carbohydrate Polymer - Water

Interactions: Sorption, Porosity and

Rheology


Project partner: Magle AB, Akzo Nobel

Pulp and Performance Chemicals AB


Development of High-Resolution

Scanning QCM-D Method for Studies

of Hydration of Biofilms

Funded by BRCB


Hydration Effects in Condensed

Biopolymer Systems

Funded by SI


Properties of Semifluorinated Alkanes

Funded by Novaliq GmbH

Project partner: Novaliq GmbH

pIs: Johan Engblom, vitaly kocherbitov

Interfacial Behaviour and Activity of

Laccase and Bilirubin Oxidase on Bare

Gold Surfaces

Funded by Mah

pI: Sergey Shleev

Viscoelastic Bioelectrocatalytic

Materials

Funded by VR

pIs: Tautgirdas Ruzgas, Thomas Arnebrant

Devices for Non-Invasive Biomedical

Sensing, Monitoring or/and Delivery


Project Partners: Galenica AB,

Novosense, Speximo AB, Bioglan AB

pI: Tautgirdas Ruzgas

Flexible and Transparent Biofuel Cells

Funded by EU, Marie Curie

Project Partner: Obducat

Technologies AB

pI: Sergey Shleev

29


Annual Report 2013

micRobiAl biofilms

Oral Implants – Nanostructures for the

Promotion of Tissue Integration and

Prevention of Infections


Project partners: Noble Biocare AB,

Promimic AB

pIs: gunnel Svensäter, Ann wennerberg

Bacterial Acid Tolerance – a New

Target for Fluoridated Milk in Caries

Prevention

Funded by the Borrow Foundation, UK

pIs: gunnel Svensäter, Julia Davies

Effect of surface characteristics on

cellular adherence and activity

PhD project, National Graduate

Research School in Odontology, funded

by Vetenskapsrådet, Mah

pIs: Marjan Dorkhan, Julia Davies, gunnel Svensäter

Biomarkers for infection

control on the biofilm level

Funded by Mah

pIs: gunnel Svennsäter, Julia Davies, Claes wickström, Jessica Neilands

Mucins and microbial biofilms

– a symbiotic relationship for health

Funded by Mah

pIs: gunnel Svennsäter, Julia Davies,

Claes wickström

The effect of probiotic lactobacilli on

development of acid adaptation in

dental biofilms


pI: Jessica Neilands

Mechanisms underlying phenotypic

adaptation in Streptococci

Funded by Mah

pIs: gunnel Svennsäter, Julia Davies

Protease inhibitors for a balanced

proteolytic activity around teeth

Funded by Mah

pIs: Bertil kinnby, Jessica Neilands

4.

Restorable and Adaptable Surfaces for

Molecular Recognition, Biosensing

and Evanescent Wave Microscopy of

Human Cells

Funded by VR

pI: Börje Sellergren

Caffeine Scavenger

Funded by Biotage AB

Project partner: Biotage AB

pI: Börje Sellergren

30


Annual Report 2013

SCIENTIFIC INSTRUMENTATION AND EqUIpMENT

opticAl micRoscopy

Contact person: peter FalkmanA Nikon Optiphot Epi-Fluorescence

microscope equipped with polarizing

filters, phase contrast and a DS-U1

digital camera. A TransferMan NK2

micro manipulator allows manipulation

of samples, such as mounting of AFM

colloidal probes, and a Linkam Analysa-

LTS350 temperature controlled stage

allows study of temperature-induced

effects, e.g. lipid phase transitions.

1.

31


Annual Report 2013

Contact person: Johan Engblom Three alternative thermostated in vitro dif-

fusion equipments are available in house,

two set ups with flow through cells (15 +

7 cells) [R.L. Bronaugh & R.F. Stewart, J.

Pharm. Sci. 74 (1985) 64–67] and 10 static

Franz cells [Franz T, J. Invest. Dermatol.

64 (1975) 190-195]. Solute diffusion over

skin, oral mucosa, nasal mucosa, nails, plant

cuticle, as well as synthetic membranes,

has been studied and assayed spectropho-

tometrically (on-line) or by HPLC-UV.

Electrical impedance spectroscopy has

been appended to the Franz cells as a tool

helping to explain diffusion properties of

solutes through these membranes.

in vitRo diffusion eQuipment

swAxd

Contact persons: peter Falkman,

Johan EngblomSmall and Wide Angle X-Ray Scattering.

Kratky compact camera with line col-

limation (slit focus), equipped with two

linear MBraun detectors (small and wide

angle). Temperature controlled sample

stages (0–70°C or 25–300°C). Powder

(semi-solid/solid) or capillary (liquid)

sample holders, as well as flow-through

capillary sample holder.

dsc

Contact person: vitaly kocherbitovDifferential scanning calorimeter DSC1

from MettlerToledo equipped with an

intracooler TC100 and HSS8 sensor.

Temperature range: –90 to +550°C. The

calorimeter can be used to study solid,

soft and liquid substances. Typical sample

masses are 2–10 mg. The DSC can be run

in modulation regime to resolve reversing

and non-reversing heat effects.

5.

2.

3.

4.

ellipsometeR

Contact persons: peter Falkman,

Thomas ArnebrantAn automated Rudolph thin film ellipso-

meter (type 43603-200E, Rudolph

Research, USA) equipped with a xenon

arc lamp as a light source; an interference

filter with UV and infrared blocking (Melles

Griot, The Netherlands) for working at a

wavelength of 4429 Å. The experimental

setup is based on null ellipsometry in the

PCSA arrangement. The components of

the ellipsometer are controlled by means

of the Ellipso software that automatically

measures the ellipsometric angles Psi and

Delta allowing the calculation of the thick-

ness, refractive index, and adsorbed mass of

the growing films. To perform experiments

in liquid media a peristaltic pump (Ole Dich

Instruments) is used. The temperature is

controlled by means of a Julabo 5B water

bath. Data analysis is performed with the

Ellipsometry software by Plamen Petrov.

32


Annual Report 2013

Qcm-d

Contact person: Sebastian BjörklundThe Q-Sense E4 quartz crystal micro-

balance is equipped with the E4 measuring

chamber that allows simultaneously run-

ning a maximum of four experiments in the

four temperature controlled cells. Changes

in frequency and dissipation are registered

by means of the E-series electronic unit

which is also responsible for the tempera-

ture control. These changes are related to

the adsorbed wet mass and the viscoelastic

properties of the films adsorbed onto the

sensor. The QCM-D is also equipped with

an electrochemistry and an ellipsometry

module enabling the simultaneous meas-

urement of the electrochemical and optical

properties respectively of the adsorbed

films. A four channels IPC peristaltic pump

(Ismatec). Data analysis is performed with

the Q-Tools software (Q-Sense).

7.

6.

8.

e-sem

Contact person: peter Falkman, Zoltan BlumScanning Electron Microscope, SEM,

Zeiss EVO LS10. Apart from standard

modern SEM features, i.e. 5 nm point-

to-point resolution at high vacuum and

fully conducting samples, the EVO LS10

also offers environmental control (hence

“EVO”). By controlled water vapor intro-

duction, with or without the added ben-

efits of a Peltier Coolstage, fully hydrated

samples can be imaged with accurate

sample temperature control; owing to the

high output LaB6 filament and the beam

sleeve technology, the loss in resolution is

only tenfold. The instrument is equipped

with the appropriate detectors and also an

INCA EDX microanalysis system.

spectRoscopic ellipsometeR uvisel hoRibA

Contact person: Marité CardenasSpectroscopic ellipsometry is a simple

and fast optical and indirect technique

that offers the possibility to determine the

average thickness of the adsorbed layer.

Ellipsometry measures the ratio of two

values, which is the amplitude ratio (Ψ=rp/

rs) and the phase difference between light

waves (Δ=Δp-Δs). These two parameters

are defined from the difference in the reflec-

tion coefficients for p- and s-polarizations

and thus in the optical properties of a

material (extinction co efficient and refrac-

tive index). We have in house an UVISEL

HORIBA spectroscopic ellipsometer cover-

ing a wavelength range of 200 – 820 nm.

33


Annual Report 2013

dls/zetA sizing system

Contact person: peter FalkmanA combined Electrophoretic Light

Scattering/Dynamic Light Scattering Zeta

potential/particle sizing system with a 5

MW 632.8 nm HeNe laser, two insert-

able electrodes for high- or low voltage,

covering possible electrical field strengths

between 0.25–250 V/cm, and an adjust-

able temperature range of 4–100°C. The

instrument has further been fitted with a

Thermaltake Bigwater 760is liquid cool-

ing system, to prevent cuvette fogging at

high temperatures.

goniometeR

Contact person: peter FalkmanKruss Goniometer DSA100 Drop shape

analyzer system for measurements of static

and dynamic contact angles. The gonio-

meter is equipped with single dosing system

DS3200/3201. Temperature is controlled

in the range 5–90 °C using TC30 measur-

ing chamber. Humidity is controlled using

HC10 humidity chamber.

Afm

Contact person: Javier Sotres,

Thomas ArnebrantA Multimode SPM equipped with a

Nanoscope VIII control unit (Bruker AXS).

The SPM is also equipped with a PicoForce

Unit, a Heater Unit, and the ScanAsyst

and Peak Force Tapping softwares (Bruker

AXS). Furthermore, a digital oscilloscope

(Tektronix TDS 2022C) and an Nikon opti-

cal microscope are coupled to the system.

impedAnce meAsuRing eQuipment

Contact person: Tautgirdas RuzgasFranz cells (Ø=0.90 cm, V=6 ml, PermeGear,

Hellertown, PA, USA) equipped with four

electrodes and connected to a potentiostat

from Ivium Technologies (Eindhoven, The

Netherlands) are used to study impedance

characteristics of membranes, including

skin, leafs, etc. Two platinum wires serve

as working and counter electrodes and two

Ag/AgCl/3M KCl electrodes from World

Precision Instruments (Sarasota, FL, USA)

are used as sensing and reference electrodes.

The equipment allows impedance measure-

ments at frequencies up to 6 MHz.

11.

9.

10.

12.

34


Annual Report 2013

RheometeR

Contact person: vitaly kocherbitov,

peter FalkmanRheometer Bohlin CVO 100 Digital

equipped with Peltier Cylinder C25. The

rheometer system is suitable for research,

product development and quality control.

The rheometer is equipped with 20 mm

parallel plate, cone 4/40 and coaxial cyl-

inders measuring systems.

soRption cAloRimeteR

Contact person: vitaly kocherbitovA double-twin sorption calorimeter for

studies of hydration of powders and soft

materials. The channel diameter is 28 mm.

The calorimeter is equipped with two sorp-

tion cells, each consist of a sorption cham-

ber (on top) and a vaporization chamber

(bottom). The cambers are connected by

tubes with varying diameters to control the

vapor flow. The double twin calorimeter

is inserted into an isothermal TAM 2277

calorimeter from Thermometric equipped

with a nanoamplifier.

pARticle electRophoResis

Contact person: Tautgirdas RuzgasParticle micro-electrophoresis apparatus

Mark II (Rank Brothers, Cambridge,

UK) consists of flat electrophoretic cell

equipped with two platinum electrodes

and optical microscope. The apparatus can

be used to determine particle electropho-

retic mobility in solution. The mobility can

be used to assess particle zeta potential and

surface charge density. Apparatus allows

measurements with particles bigger than

0.3 µm in diameter.

lAngmuiR suRfAce bAlAnce

Contact person: Johan Engblom,

Marité CardenasKSV 5000 is a programmable Langmuir

and Langmuir-Blodgett instrument for

automated Langmuir film experiments and

for deposition of normal or alternating

multilayers onto solid substrates. Surface

pressure is measured using the Wilhelmy

plate method.

dRop volume AppARAtus

Contact person: Tautgirdas RuzgasDrop Volume Tensiometer TVT 2 consists

of step motor driven syringe including a

needle for droplet formation, setup for

optical droplet falling registration, and

software to operate hardware and calculate

surface tension of liquid. This apparatus is

used for determination of surface tension

of liquids.

14.

13.

15.

16.

17.

35


Annual Report 2013

inteRfeRometeR

Contact persons: Ryo Jimbo, Ann wennerbergWhite light microscopy, horizontal reso-

lution 0.3 µm, vertical 0.1 nm, equipped

with a CCD camera, operating also in

phaseshift mode. The main application is

topographical characterisation on the µm

and sub-µm level. AFM Park Instrument,

surface characterization in the nm level.

Real time PCR- Gene expression

novAsinA lAbmAsteR-Aw

Contact person: Johan EngblomAn instrument for water activity measure-

ments. Accuracy: 0.003 aw (0.3%RH),

range: 3 to 100%RH.

lc-ms

Contact person: Börje SellergrenZQ2000 MS system with 2795 LC and

2996 PDA. The Waters ZQ 2000 LCMS

System is based on the Micromass-ZQ 2000

Mass Selective Detector. This is a robust sin-

gle quadrupole mass spectrometer, offering

characteristic performance for laboratories

that require nominal mass resolution for

mass confirmation applications. This system

has a mass range (amu) up to 2000 daltons.

Easily qualify and quantify small molecules

in a flash with the highly sensitive system.

Both inlet probes are quickly and easily

interchangeable without breaking vacuum.

Flow rates allowed are 5–1000 µl/min (ESI)

and 200–2000 µl/min (APCI).

lc -uv/dAd

Contact person: Börje SellergrenTwo Waters LC-UV/DAD 2795/2996

(LCDAD) systems. This robust HPLC

apparatus allows flow-rates from 50ul/

min to 5ml/min for use with 2.1 mm ID

columns and larger. The autosampler

accepts standard 96/384 well plates with

programmable temperature control from

4 to 40°C.A heated column compartment

provides temperatures from 5 degrees

above ambient to 65°C. A range of LC

detectors, like a dual wavelength- or PDA

UV detector is optionally available.

fluoRchem e

Contact person: Anette gjörloff wingrenHigh-performance Western blot and gel

imaging. A self-contained digital dark-

room, compact design with state-of-the-art

CCD optical technology, an integrated

computer and simplified touch screen

control. The FluorChem E is built with

imaging speeds and a dynamic range that

surpass film for low femtogram detection

of proteins and DNA in chemiluminescent,

colorimetric and UV fluorescent gels and

blots.

20.

18.

19.

21.

22.

36


Annual Report 2013

biofilm foRmAtion, including AdheRence, detAchment, populAtion diveRsity And phenotypic shift

Contact person: gunnel SvensäterEpifluorescence microscope

Confocal laser scanning microscope

Flow cells with controlled continuous flow

Cell cultures

puRificAtion of mucins And pRoteins

Contact person: gunnel SvensäterFPLC

Preparative electrophores

Ultracentrifuge

pRoteomics, including RAdioActive lAbelling of pRoteins

Contact person: gunnel SvensäterMultiphor

Two-dimensional electrophoresis

Sonicator

identificAtion of pRoteins

Contact person: gunnel SvensäterImmunohistochemistry

Western blots

ELISA

electRochemicAl eQuipment

Contact person: Sergey Shleev6 potentiostats/galvanostats from Autolab,

BAS, and Ivium. Unique spectroelec-

trochemical system (home-made 10 L

spectroelectrochemical cell, spectrometer

HR 4000 CG-UV-NIR and light source

DH-2000 from Ocean Optics). Flow-

injection analysis

micRobiAl diAgnostics; species And stRAin levels

Contact person: gunnel SvensäterAutoclaves

Anaerobic chamber

Fluorostar

Incubators

Spectrophotometer

PCR

Centrifuges

24.

23.

25.

26.

27.

28.

37


Annual Report 2013

COLLABORATIVE pARTNERS OF THE CENTER

industRy collAboRAtoRs

ACO Hud Nordic AB

Acreo AB

Akzo Nobel Pulp and Performance

Chemicals AB

Akzo Nobel Surface Chemistry AB

Bioglan AB

Biotage AB

Camurus AB

CapSenze HB

Corigo AB

Dentsply

Dräger AG, Lübeck, DE

Enzymatica AB

Eviderm AB

Galenica AB

GT Septech, Oslo, NO

In vitro Plant-tech AB

Indienz AB

Magle AB

Medeon AB

Medicon Valley Alliance

Nares AB

Nobel Biocare AB

Novaliq GmbH, DE

Novosense AB

N-Zyme Biotech, Darmstadt, DE

Obducat Technologies AB

Orbital Systems AB

Promimic AB

SP AB

Speximo AB

Thermo Fischer, UK

Zelmic AB

1.

38


Annual Report 2013

AcAdemic collAboRAtoRs

collAboRAtions with otheR univeRsities And ReseARch institutions

in sweden

Professor Anders Rosén, Cell Biology, university of Linköping, Linköping

Professor Artur Schmidtchen, Lund university

Professor Chris Anderson, Dermatology, university of Linköping, Linköping

Professor em. Kåre Larsson, Camurus Lipid Research Foundation, Lund

Professor Emma Sparr, physical Chemistry 1, Lund university

Professor Fredrik Tiberg, Camurus AB, Lund

Professor Gunnar Dahlén, Dept of Oral Microbiology, university of gothenburg

Professor Ian Nicholls, Lineaus university, kalmar

Professor Jens Schouenborg, Neuronano Research Center, Medical Faculty, Lund university

Professor Knut Irgum, uME, umeå

Professor Kristian Reisbeck, Dept of Medical Microbiology, Lund university

Professor Lars Montelius, Solid State physics, Lund university

Professor Lo Gorton, Biochemistry, Lund university

Professor Marie Wahlgren, Food Technology, LTH, Lund university

Professor Mark Rutland, The Royal Institute of Technology and Sp AB, Stockholm

Professor Martin Andersson, Dept Applied Chemistry, Chalmers university of Technology

Professor Martin Malmsten, pharmacy, uppsala university

Professor Maud Langton, Swedish university of Agricultural Sciences, uppsala

Professor Olle Söderman, physical Chemistry, Lund university

Professor Pentti Tengvall, Dept Biomaterials, Sahlgrenska Academy, göteborg university

Professor Per Claesson, The Royal Institute of Technology and Sp, Stockholm

Professor Per Wollmer, Clinical Sciences, Lund university

Professor Staffan Nilsson, Lund university

Professor Thomas Laurell, Lund university

Professor Tomas Albrektsson, Dept Biomaterials, Sahlgrenska Academy, göteborg university

2.

39


Annual Report 2013

Professor Tommy Nylander, physical Chemistry, Lund university

Professor Viveka Alfredsson, physical Chemistry, Lund university

Assoc. Professor Malin Sjöö, Food Technology, Lund university

Assoc. Professor Marilyn Rayner, Food Technology, Lund university

Assoc. Professor Ola Bergendorff, Dermatology, Lund university

Assoc. Professor Eva Blomberg, The Royal Institute of Technology and Sp AB, Stockholm

Assoc.Professor Peter Siesjö, Department of Clinical Sciences, BMC, Lund university

Per Rabe, Specialist Clinic for Oral Health Care, Hallands Läns Landsting, Halmstad

Doctor Andreas Sonesson, Lund university

Doctor Anna Westerlund, Odontology, gothenburg university

Doctor Annika Krona, The Swedish Institute for Food and Biotechnology, gothenburg

Doctor Birgit Brandner, Sp kM AB, Stockholm

Doctor Christer Wingren, LTH, Lund university

Doctor Jenny Liao Persson, Center for Molecular pathology, Lund university

Doctor Karina Persson, Inst. of Odontology, umeå university

Doctor Maria Lövenklev, The Swedish Institute for Food and Biotechnology, gothenburg

Doctor Marie Skepö, Dept of Theoretical Chemistry, Lund university

Doctor Robert Corkery, The Royal Institute of Technology

Doctor Björn Svensson, ÖTL, Specialist docent, Clinic for Oral Health Care, Örebro Läns Landsting, Örebro

Doctor Sylvio Haas, MAX, Lund university

Doctor Tomás Plivelic, MAX lab, Lund university

Brånemark Clinic, gothenburg

40


Annual Report 2013

inteRnAtionAl collAboRAtions

Professor Anja Boisen and post-doc Robert Burger, DTu Nanotech, Copenhagen

Professor Ben J. Boyd, Monash Institute of pharmaceutical Sciences, Monash university, Australia

Professor Christopher Exley, keele university, uk

Professor David Beighton, guy’s kings and St Thomas’ Dental Institute, Joint Microbiology Research unit, London, uk

Professor Deirdre Devine, Leeds Dental Institute, uk

Professor Dennis Cvitkovitch, Dept of Microbiology, university of Toronto, Canada

Professor Dietmar Haltrich, universität für Bodenkultur wien, Austria

Professor Doctor Alexander Yaropolov, Institute of Biochemistry, Moscow, Russia

Professor ECI Veerman, Department of periodontology and Oral Biochemistry, Academic Centre for Dentistry Amsterdam (ACTA)

Professor Edmond Magner, university of Limerick, Ireland

Professor Gediminas Niaura, vilnius university, Lithuania

Professor Gordon Proctor, kings College, London, uk

Professor Janusz Pawlitzyn, university of waterloo, Canada

Professor Jeannine Brady, Dept of Oral Biology, university of Florida, gainesville, uSA

Professor Jens Wiltfang, university of Essen, germany

Professor Jon E. Dahl, NIOM, Oslo, Norway

Professor Jukka Meurman, Dept of Oral and Maxillofacial Diseases, Helsinki university Central Hospital, Helsinki, Finland

Professor Juozas Kulys, Inst. of Biochemistry, vilnius, Lithuania

Professor Kamal Mustafa, university of Bergen, Norway

Professor Katrin Markus, university of Bochum, germany

Professor Leon Rubesaet, uIO, Oslo, Norway

Professor Mark Herzberg, Department of Microbiology, university of Minnesota, Minneapolis, uSA

Professor Markus Wenk, National university of Singapore, Singapore

Professor Ole Jensen, SDu, Odense, Denmark

Professor Peter Cormack, university of Strathclyde, uk

41


Annual Report 2013

Professor Phil Bartlett, The university of Southampton, uk

Professor Phil Marsh, Health protection Agency, porton Down, uk

Professor Robert Baier, university of Buffalo, Industry/university Cooperative Research Center for Biosurfaces, Buffalo, uSA

Professor Takashe Sawase, Dept prosthodontics, university of Nagasaki, Japan

Professor Vladimir O. Popov, kurchatov NBIC Centre, Moscow, 123182, Russia

Professor Wolfgang Schuhmann, Ruhr-universität Bochum, germany

Assoc. Professor David Thornton, university of Manchester, uk

Assoc. Professor Marie Ranson, School of Biological Sciences, Scientific Director – Cancer, Illawarra Health and Medical Research Institute, university of wollongong, wollongong, Australia

Doctor Anne Meyer, university of Buffalo, Industry/university Cooperative Research Center for Biosurfaces, Buffalo, uSA

Doctor Antonio L. De Lacey, Consejo Superior de Investigaciones Científicas, Bioelectrocatalysis group, Spain

Doctor Donald Leech, National university of Ireland, galway, Ireland

Doctor Humberto Schwartz, Sao paolo, Brasilien

Doctor Jan-Tore Samuelsen, NIOM, Oslo, Norway

Doctor Jelena Kosoric, Centre for Oral growth and Development, queen Mary’s School of Medicine and Dentistry, university of London, uk

Doctor Miguel Alcalde, Consejo Superior de Investigaciones Científicas, Applied Biocatalysis group, Spain

Doctor Paulo G. Coelho, Department of Biomimetics, New york university, uSA

Doctor Sergei Lobov, Browitt Nanoparticle Lab, Dept of Applied Mathematics, Australian National university, Canberra, Australia

Doctor Yuusuke Kozai, Department of Radiology, kanagawa Dental university

Assoc. Professor Tim Tolker-Nielsen, university of Copenhagen, Denmark

PhD student Nayab Chaudhury, kings College, London, uk

National Research Centre kurchatov Institute, Moscow, Russia

42


Annual Report 2013

wORkSHOpS AND CONFERENCES ORgANISED BY THE CENTER

The Center launched the 9th Annual Workshop in October. The focus this year was ‘Biomarkers’ and the event attracted 90 participants from different universities as well as industry. Read more about the workshop in the article below.

Claes Wickström and Jessica Neiland

were responsible for organising the 8th

International Conference on Problem-

Based Learning in Dentistry, which was

held at the Faculty of Odontology in

May. The Faculty of Odontology has been

working with problem-based learning since

the early 1990s and this was the second

time that the Faculty has organised the

conference. The conference attracted 91

participants from 19 countries.

The Topical Science Cluster – a joint

venture between the Center and LPR@

LU – Pharmaceutical Research at Lund

University – organised three well-received

workshops during 2013.

• “A meeting between clinical research

and technology around our shared

interface: the skin”, held at the

Department of Dermatology and

Venereology, Skåne University

Hospital

• “Human Interfaces: Structure and

transport in skin and in lung alveoli”,

held at the Department of Physical

Chemistry, Lund University

• “From Molecule to Man!” held at

ASA Herrgård, Lammhult.

The three workshops attracted both

national and international speakers.

Ann Wennerberg and Gunnel Svensäter,

who are coordinators for the National

Graduate Research Schools in Odontology,

organised the summer conference

“Approaches to clinical research”, which

took place at Ystad Saltsjöbaden in June.

Börje Sellergren, who is coordinating

the EU FP7 Marie Curie Initial Training

Network PEPMIP – Robust affinity mate-

rials for applications in proteomics and

diagnostics, organised the business devel-

opment seminar “From idea to product”

together with Biotage in Lund as part of

training activities in the network. Börje

has also been involved in arranging the

following:

• The Graduate Student Symposium

at Belfast University, which attracted

44 participants and included lectures

on Molecular Imprinting Technology

delivered by graduate students.

• The workshop “Monolithic Materials

and Synthesis and Characterisation

of Porous Materials”, held at Umeå

University. The event attracted 36

participants.

• The 5th International HILIC Day,

held at Umeå University. The meeting

attracted 40 participants.

Also worth mentioning is Johan

Engblom’s participation in the Tv pro-

gramme “vetenskap” with Madeleine

westin on Tv4 play

http://www.tv4play.se/program/

vetenskap?video_id=226369.

43


Annual Report 2013

biomARKeRs – 9th AnnuAl woRKshop of bRcb

The annual workshop was held on

October 3–4 and addressed technical

aspects and applications of biomarkers.

Some 80 participants from academia and

industry took part.

“I’m extremely interested in research and

this is the third workshop in a row that I

have attended,” says Helena Ekdahl from

Labjoy AB.

The workshop, the ninth in succession,

focused on techniques for analysing

biomarkers.

The speakers included Dr Sarah Fredriks-

son, President of Genovis AB, who spoke

about nanostructures that can improve

medical image analysis, such as MRI. She

emphasised how important the design of a

nanostructure is to the end result. Professor

György Marko-Varga, head of the Division

of Clinical Protein Science and Imaging at

Lund University and the person respon-

sible for the development of biomarkers

in the Skåne Region, spoke about the

LUNDHEARTGENE project. This is a

large biobank with patient samples and

György spoke about how, based on this,

an analysis has been made of cardiovas-

cular markers in blood plasma. The aim

is to try to find new proteins as a goal in

pharmaceutical development.

Professor Börje Sellergren at BRCB at

Malmö University, spoke about molecular

imprinting. In simple terms, this involves

making imprints in plastic that can then

bind molecules, similar to the way anti-

bodies function. Associate Professor Asli

Silahtaroglu from Copenhagen University

spoke about microRNAs, which are short

RNA strands that control different gene

expressions. Asli spoke about being able

to detect specifically microRNAs in tissue

sections. Many microRNAs function as

diagnostic markers for different diseases,

such as cancer. Circulating microRNAs

are also a new potential generation of

biomarkers that can be used to discover

heart diseases.

One of the participants, Helena Ekdahl

from Labjoy AB, named Trippelhelix, the

new innovation model for collaboration

between academia, industry and the public

sector, and stated that it would be very

much a win-win situation if everyone is

linked together.

The workshop offered opportunities to

create new contacts. “This year’s work-

shop has been extremely interesting and

taking part has been a good way to keep

up to date on research,” Helena concludes.

1.

44


Annual Report 2013

SEMINARS AT THE CENTER

Naghi Momeni, Medeon, Malmö, “Autism Spectrum Disorder (ASD)”

Nina Oestergaard Knudsen, LEO Pharma, “Lipid nanoparticles for treatment of skin diseases with barrier impairment”

Petri Gudmundsson, Mah, “Pedagogic model – constructive alignment”

Marius Dagys, Institute of Biochemistry, Vilnius “New oxidoreductases for development of biofuel cells”

Jonas Carlstedt, Mah, “Hydration of starch: sorption, glass transition and structure”

Börje Sellergren, Mah, “Molecularly imprinted polymers”

Hala Gari, Mah, “Characterisation of biological membranes by impedance spectroscopy”

Gesche Graf, Mah, “Development of a high-resolution scanning QCM-D technique”

Charlotta Turner, Lund University, “Department of Chemistry, Green Technology Group”

Patrick Seumo, University of Yaounde I, Yaounde, Cameroon, “Electrochemical quantifi-cation of protein concentration”

Ben J. Boyd, Monash University, Australia, “Controlling structural transformation in self assembled lipid liquid crystals for drug delivery”

Ausra Linkeviciute, Center for Physical Science and Technology “Food and drugs: different roles of nanotechnology”

Per Hyldgaard, Microtechnology and Nanoscience, MC2, Chalmers, “A parameter-free computational theory of biomolecular interactions”

Thomas Zemb, Institute for Separation Chemistry of Marcoule, France, “Surfactant-free microemulsions”

Peter Spégel, Unit of Molecular Metabolism, Lund University Diabetes Centre, “Metabolomics in medical research”

Gordon Proctor, Kings College, UK, “Comparison of saliva from dry mouth patients with saliva from healthy individuals”

Nayab Chaudhury, Kings College, UK “Comparison of saliva from dry mouth patients with saliva from healthy individuals”

Lars-Åke Johansson, Halmstad “On minimally invasive approaches to sinus lift procedures”

45


Annual Report 2013

Maria Pigg, Mah, “When should we save the tooth and when is an implant the better solution?”

Lory Melin-Svanborg and Nils Lövgren, Mah, “How shall we evaluate endodontically treated teeth at prosthetic therapy planning?”

Camilla Ahlgren, Mah, “Material reactions”

Rolf Liedholm, Mah, “Mucus membrane changes due to prosthetic treatments”

Barbro Swartz, Örebro University, ”Juvenile Prosthodontics”

Per Vult von Steyern, Mah, “Ceramics”

Gunilla Nordin Fredrikson, Faculty of Medicine, Lund University, “Immune biomarkers

Karin von Wachenfeldt, Truly Translational AB, “Design of clinical studies for biomarker evaluation”

Thuy Tran, Bioimagning Center, Lund University, “In Vivo Detection of Biomarkers using PET/SPECT/CT-imaging modalities”

Sarah Fredriksson, Genovis AB, “Using Nanostructures as Multimodal Contrast Agents in Medical Imaging”

György Marko-Varga, Clinical Protein Science & Imaging, Lund University, “Biomarker Diagnosis by Protein Sequence assay Develoments utilizing Mass Spectrometry”

Börje Sellergren, Faculty of Health and Society, Malmö University, “Molecularly imprinted polymers in biomarker analysis”

Ali Moazzami, Metabolomics, SLU, “Metabolomics a tool to identify the biomarkers of physiological status”

Malin Lindstedt, Immunotechnology, Lund University, “Signature biomarker analy-sis for prediction of skin sensitizers using a cell- based in vitro alternative to animal experimentation”

Asli Silahtaroglu, Faculty of Health Sciences, University of Copenhagen, “In situ detection of members of a microRNA family using novel probe chemistries”

Joyce Carlson, MD, Clinical Chemistry and Pharmacology, Lund University, “Biobanking for Biomarkers”

Simon Ekström, Electrical Measurements, Lund University, “Microfluidic sample handling for Biomarker Analysis”

Bo Åkerström, Faculty of Medicine, Lund University, “Prediction and diagnosis of preeclampsia”

Maria Falck Miniotis, Faculty of Health and Society, Mah, “Digital holographic micros-copy for non-invasive monitoring of cellular responses to cancer therapy”

46


Annual Report 2013

pHD THESES SUpERVISED BY CENTER MEMBERS

theses defended: Alma MašićLund university (Supervisors: per Ståhle,

Johan Helsing)

“Investigation of a biofilm reactor model

with suspended biomass”

Dissertation 2013-03-22

Marija Jankunec

vilnius university (Supervisor: Justas

Barauskas)

”Investigation of properties of lipid

liquid crystalline drug carriers and their

interactions with model cell membranes”


Sebastian Björklund

Lund university (Supervisors: Emma Sparr at

Lu, Johan Engblom Mah, krister Thuresson

at Camurus AB)

“Skin hydration – How water and osmolytes

influence biophysical properties of stratum

corneum”


Yana Znamenskaya

Malmö university (Supervisors: vitaly

kocherbitov, Johan Engblom)

“Effect of hydration on thermodynamic, rheological and structural properties of mucin” Dissertation 2013-09-12

theses in pRogRess: Anton Fagerström

Malmö university (Supervisors: Johan

Engblom, vitaly kocherbitov, karin

Bergström at Akzo Nobel Surface Chemistry

AB)

“Bioavailability of active ingredients used in

agriculture”

Start: 2009

Marjan Dorkhan

Malmö university (Supervisors: Julia Davies,

gunnel Svensäter)

“Activities of microbial biofilms on bioactive

implant surfaces”

Start: 2009

Magnus Falk

Malmö university (Supervisors: Sergey

Shleev, Tautgirdas Ruzgas)

“Direct electron transfer based biofuel cells”

Start: 2009

Tuerdi Maymaytilli

LTH (Supervisors: per Ståhle, Christina

Bjerkén)

“Influence of plastic deformation on the

formation and growth of embritteling metal

hydride’s”

Start: 2009

Liselott Ellmarker-Löfquist

Malmö university (Supervisors: Liselott

Lindh, Mats kronström,)

”A Clinical and subjective evaluation of

single implant treatments. A retroscpective

study.”

Start: 2009

Mariko Hayashi, Malmö University

(Supervisors: Liselott Lindh, Ann

wennerberg, Ryo Jimbo)

“Biological Responses to Amphiphilic Nano-

size Structures”

Start: 2010

47


Annual Report 2013

Peter Lamberg

Malmö university (Supervisors: Tautgirdas

Ruzgas, Thomas Arnebrant)

“Three-dimensional (3D) bioelectrochemical

systems assembled from nanoparticles”

Start: 2010

Cathrine Albér

Malmö university (Supervisors: Johan

Engblom, vitaly kocherbitov, Marie Lodén,

Robert Corkery)

“Humectants and their effects on skin barrier

function – a mechanistic approach”

Start: 2010

Zahra El-Schich

Malmö university (Supervisors: Anette

gjörloff wingren, Anna ketelsen, gunilla

Nordin Fredrikson)

“Microscopy and digital holography for

real-time applications and imaging: studying

the cellular and molecular mechanisms of

inflammatory cells and cancer cells”

Start: 2011

Celina Wierzbicka

Malmö university (Supervisor: Börje

Sellergren, Thomas Arnebrant):

“New approaches to molecularly imprinted

micro- and nanoparticles for posttrans-

lationally modified proteins and protein

fragments”

Start: 2012

Dmitrii Pankratov

Malmö university (Supervisors: Sergey

Shleev and Zoltan Blum)

“Function of bioelectronic devices in “extra

vivo” situations”

Start: 2012

Mark Galat

Malmö university (Supervisor: Börje

Sellergren, Thomas Arnebrant)

“Development and characterisation of

molecularly imprinted polymer receptors tar-

geting pathogenic and biomarker peptides

related to neurodegenerative diseases”

Start: 2013

Sing Yee Yeung

Malmö university (Supervisors: Börje

Sellergren, Thomas Arnebrant)

“Restorable and adaptable surfaces for

molecular recognition, biosensing and eva-

nescent wave microscopy of human cells”

Start: 2013

Pär Johansson

Malmö university (Supervisors: Ann

wennerberg, Ryo Jimbo)

“A unique surface modification to improve

bioactivity of a polymer, pEEk, for ortho-

paedic and dental applications”

Start: 2012

Silvia Galli

Malmö university (Supervisors: Ryo Jimbo,

Ann wennerberg)

“On the effect of magnesium incorporated

biomaterials for bone regeneration”

Start: 2012

Francesca Cecchinato

Malmö university (Supervisors: Ryo Jimbo,

Ann wennerberg)

“Characterisation of magnesium alloys for

oral & maxillofacial applications”

Start: 2012

Ramesh Chowdhary


wennerberg, Liselott Lind, Lennart Carlsson)

“On efficacy of implant thread design for

bone stimulation”

Start: 2009

48


Annual Report 2013

Jonas Anderud


wennerberg, Sten Isaksson, peter

Abrahamsson)

“guided bone regeneration using ceramic

spacemaintaining devices”

Start: 2011

Michael Braian

Malmö university (Supervisor: Ann

wennerberg)

“On the influence of misfit on implant

supraconstructions”

Start: 2008

Deyar Mahmood


wennerberg, per vult von Steyern)

“All ceramic bridges, choice of material used

for supporting tooth analogues, Connector

dimension and design, length of units and

different veneering techniques”

Start: 2009

Ricardo Trindade


wennerberg, Ryo Jimbo, Tomas Albrektsson)

“On Foreign Body Reactions Around Oral

Implants”

Start: 2013

Vida Krikstolaityte

vilnius university (Supervisors: Arunas

Ramanavicius at vilnius university,

Tautgirdas Ruzgas)

“Application of nanotechnology in design of

enzymatic fuel cells”

Start: 2009

Patrick Seumo

university of yaoundé I, yaounde,

Camoroon (Supervisors: Ngameni

Emmanuel at university of yaoundé I,

Nanseu Njiki Charles peguy at university of

yaoundé I, Tautgirdas Ruzgas)

“Application of nanoparticles in

electroanalysis”

Start: 2010

49


Annual Report 2013

LIST OF pUBLICATIONS AT THE CENTER FROM 2013

Pihl M, Arvidsson A, Skepö M, Nilsson M, Givskov M, Tolker-Nielsen T, Svensäter G, Davies JR. Biofilm formation by Staphylococcus epidermidis on peritoneal dialysis catheters and the effects of extracellular products from Pseudomonas aeruginosa. Pathogens and Disease 2013;67:192-198.

Fransson H, Petersson K, Davies JR. Effects of bacterial products on the activity of odontoblast-like cells and their formation of type 1 collagen. Int Endod J 2013; doi: 10.1111/iej.12160. [Epub ahead of print].

Nylander Å, Svensäter G, Senadheera DB, Cvitkovitch DG, Davies JR, Persson K. Structural and functional analysis of the N-terminal domain of the Streptococcus gordonii adhesin Sgo0707”. PloS ONE 2013;8(5):e63768.

Dorkhan M, Svensäter G, Davies JR. Salivary pellicles on titanium and their effect on metabolic activity in Streptococcus oralis. BMC Oral Health 2013;13:32.

Wickström C, Chávez de Paz LE, Davies JR, Svensäter G. Surface-associated MUC5B mucins promote protease activity in Lactobacillus fermentum biofilms. BMC Oral Health 2013;13:43.

Pankratov, Dmitry; Zeifman, Yulia; Morozova, Olga; Shumakovich, Galina; Vasil’eva, Irina; Shleev, Sergey; Popov, Vladimir; Yaropolov, Alexander. A comparative study of biocathodes based on multiwall carbon nanotube buckypapers modified with three different multicopper oxidases. Wiley-VCH Verlag. 2013; Electroanalysis; 5 1143-1149. ISSN: 1040-0397 DOI: http://dx.doi.org/10.1002/elan.201200516

Jujic, Amra; Leosdottir, Margret; Östling, Gerd; Gudmundsson, Petri; Nilsson, Peter; Melander, Olle; Magnusson, Martin. A genetic variant of the atrial natriuretic peptide gene is associated with left ventricular hypertrophy in a non-diabetic population – the Malmo preventive project study. BioMed Central. 2013; BMC Medical Genetics; 64 1-6. ISSN: 1471-2350 DOI: http://dx.doi.org/10.1186/1471-2350-14-64

Ohlsson, Lars; Exley, Christopher; Darabi, Anna; Sandén, Emma; Siesjö, Peter; Eriksson, Håkan. Aluminium based adjuvants and their effects on mitochondria and lysosomes of phagocytosing cells. Elsevier. 2013; Journal of Inorganic Biochemistry; 128 229-236. ISSN: 1873-3344 DOI: http://dx.doi.org/10.1016/j.jinorgbio.2013.08.003

Kocherbitov, Vitaly. Application of scanning methods to distinguish between entropy and enthalpy driven phase transitions Elsevier. 2013; Current Opinion in Colloid & Interface Science; 6 510-516. ISSN: 1359-0294 DOI: http://dx.doi.org/10.1016/j.cocis.2013.11.003

Andoralov, Viktor; Falk, Magnus; Suyatin, Dmitry; Granmo, Marcus; Sotres, Javier; Ludwig, Roland; Popov, Vladimir; Schouenborg, Jens; Blum, Zoltan; Sergey, Shleev. Biofuel cell based on microscale nanostructured electrodes with inductive coupling to rat brain neurons. Nature Publishing Group. 2013; Scientific Reports; 3 art. 3270. ISSN: 2045-2322 DOI: http://dx.doi.org/10.1038/srep03270

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Annual Report 2013

Falk, Magnus; Narvaez Villarrubia, Claudia; Babanova, Sofia; Atanassov, Plamen; Shleev, Sergey. Biofuel cells for biomedical applications : colonizing the animal kingdom Wiley-VCH Verlag. 2013; ChemPhysChem; 10 2045-2058. ISSN: 1439-4235 DOI: http://dx.doi.org/10.1002/cphc.201300044

Mate, Diana; Gonzalez-Perez, David; Falk, Magnus; Kittl, Roman; Pita, Marcos; De Lacey, Antonio; Ludwig, Roland; Shleev, Sergey; Alcalde, Miguel. Blood tolerant laccase by directed evolution. Elsevier. 2013; Chemistry & Biology; 2 223-231. ISSN: 1074-5521 DOI: http://dx.doi.org/10.1016/j.chembiol.2013.01.001

Alm, Kersti; El-Schich, Zahra; Falck Miniotis, Maria; Gjörloff Wingren, Anette; Janicke, Birgit; Oredsson, Stina; Mihaylova, Emilia (ed). Cells and holograms: holograms and digital holographic microscopy as a tool to study the morphology of living cells. Intech. 2013; Holography : basic principles and contemporary applications 335-351. ISBN: 978-953-51-1117-7 DOI: http://dx.doi.org/10.5772/54505

Fagerström, Anton; Kocherbitov, Vitaly; Westbye, Peter; Bergström, Karin; Mamontova, Varvara; Engblom, Johan Characterization of a plant leaf cuticle model wax, phase behaviour ofmodel wax-water systems. Elsevier. 2013; Thermochimica Acta; 42-52. ISSN: 0040-6031 DOI: http://dx.doi.org/10.1016/j.tca.2013.08.025

Fagerström, Anton; Kocherbitov, Vitaly; Westbye, Peter; Bergström, Karin; Mamontova, Varvara; Engblom, Johan; Castelani, Priscila (ed); Stock, David (ed); Puente Moran, Diana (ed). Composition of plant leaf wax, phase behavior of major components and effects of hydration. ISAA 2013 – 10th International Symposium on Adjuvants for Agrochemicals, Foz-do-Iguaco, Brazil, 22-27/4 2013. ISAA Society. 2013; Proceedings of the 10th International Symposium on Adjuvants for Agrochemicals (ISAA 2013); 257-262. ISBN: 978-90-815702-0-6

Halhalli, Mahadeo; Sellergren, Börje. Cover and uncover: chiral switching exploiting templating and layer-by-layer grafting. Royal Society of Chemistry. 2013; Chemical Communications; 64 7111-7113. ISSN: 1359-7345 DOI: http://dx.doi.org/10.1039/c3cc41989d

Graf, Gesche; Kocherbitov, Vitaly. Determination of Sorption Isotherm and Rheological Properties of Lysozyme Using a High-Resolution Humidity Scanning QCM-D Technique American Chemical Society ACS. 2013; The Journal of Physical Chemistry B;34 10017–10026. ISSN: 1520-6106 DOI: http://dx.doi.org/10.1021/jp404138f

Salaj-Kosla, Urszula; Poller, Sascha; Schuhmann, Wolfgang; Shleev, Sergey; Magner, Edmond. Direct electron transfer of Trametes hirsuta laccase adsorbed at unmodified nanoporous gold electrodes. Elsevier. 2013; Bioelectrochemistry; 15-20. ISSN: 1567-5394 DOI: http://dx.doi.org/10.1016/j.bioelechem.2012.11.001

Znamenskaya, Yana. Effect of hydration on thermodynamic, rheological and structural properties of mucin. Malmö University, Faculty of Health and Society. 2013; 21. ISBN: 978-91-7104-514-0 (pdf) ISBN: 978-91-7104-513-3 (print) ISSN: 1653-5383

Fagerström, Anton; Kocherbitov, Vitaly; Ruzgas, Tautgirdas; Westbye, Peter; Bergström, Karin; Engblom, Johan. Effects of surfactants and thermodynamic activity of model active ingredient on transport over plant leaf cuticle. Elsevier. 2013; Colloids and Surfaces B: Biointerfaces; 572-579. ISSN: 0927-7765 DOI: http://dx.doi.org/10.1016/j.colsurfb.2012.11.011

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Annual Report 2013

Albèr, Cathrine; Brandner, Birgit; Björklund, Sebastian; Billsten, Peter; Corkery, Robert; Engblom, Johan. Effects of water gradients and use of urea on skin ultrastructure evaluated by confocal Raman microspectroscopy. Elsevier. 2013; BBA - Biomembranes; 11 2470-2478. ISSN: 0005-2736 DOI: http://dx.doi.org/10.1016/j.bbamem.2013.06.011

Ignatenko, O. V.; Sjölander, A.; Hushpulian, D. M.; Kazakov, S. V.; Ouporov, I. V.; Chubar, T. A.; Poloznikov, A. A.; Ruzgas, Tautgirdas; Tishkov, V. I.; Gorton, L.; Klyachko, N. L.; Gazaryan, I. G. Electrochemistry of chemically trapped dimeric and monomeric recombinant horseradish peroxidase. Science and Engineering Publishing Company. 2013; Advances in Biosensors and Bioelectronics; 3 25-34. ISSN: 2326-473X

Sotres, Javier; Arnebrant, Thomas. Experimental Investigations of Biological Lubrication at the Nanoscale : The Cases of Synovial Joints and the Oral Cavity. MDPI. 2013; Lubricants;4 102-131. DOI: http://dx.doi.org/10.3390/lubricants1040102

Andoralov, Viktor; Shleev, Sergey; Arnebrant, Thomas; Ruzgas, Tautgirdas. Flexible micro(bio)sensors for quantitative analysis of bioanalytes in a nanovolume of human lachrymal liquid. Springer. 2013; Anal Bioanal Chem; 11 3871-3879. ISSN: 1618-2642

Wan, Wei; Biyakal, Mustafa; Wagner, Ricarda; Sellergren, Börje; Rurack, Knut. Fluorescent sensory microparticles that “light-up” consisting of a silica core and a molecularly imprinted polymer (MIP) shell. Wiley-VCH Verlag. 2013; Angewandte Chemie International Edition; 27 7023-7027. ISSN: 1433-7851 DOI: http://dx.doi.org/10.1002/anie.201300322

Björklund, Sebastian; Engblom, Johan; Thuresson, Krister; Sparr, Emma. Glycerol and urea can be used to increase skin permeability in reduced hydration conditions. Elsevier. 2013; European Journal of Pharmaceutical Sciences; 50 638-645. ISSN: 0928-0987 DOI: http://dx.doi.org/10.1016/j.ejps.2013.04.022

Pankratov, Dmitry; Falkman, Peter; Blum, Zoltan; Shleev, Sergey. Hybrid electric power device for simultaneous generation and storage of electric energy. Royal Society of Chemistry. 2013; Energy and Environmental Science; e-pub ahead of print. DOI: http://dx.doi.org/10.1039/C3EE43413C

Kocherbitov, Vitaly; Latynis, Jekaterina; Misiunas, Audrius; Barauskas, Justas; Nuaura, Gediminas. Hydration of Lysozyme Studied by Raman Spectroscopy American Chemical Society. 2013; The Journal of Physical Chemistry B; 17 4981–4992. ISSN: 1520-6106 DOI: http://dx.doi.org/10.1021/jp4017954

Svensson, Olof; Sotres, Javier; Barrantes, Alejandro; Piñeiro, Ángel (ed); Ruso, Juan M. (ed). Investigating protein interactions at solid surfaces : in situ, non-labeling techniques. Wiley. 2013; Proteins in solution and at interfaces : methods and applications in biotechnology and materials science 113-138. ISBN: 9780470952511

Tassidis, Helena; Brokken, Leon JS; Jirström, Karin; Bjartell, Anders; Ulmert, David; Härkönen, Pirkko; Gjörloff Wingren, Anette. Low expression of SHP-2 is associated with less favorable prostate cancer outcomes. Springer. 2013; Tumor Biology; 2 637-642. ISSN: 1010-4283 DOI: http://dx.doi.org/10.1007/s13277-012-0590-1

Falk, Magnus; Andoralov, Viktor; Silow, Maria; Toscano, Miguel; Shleev, Sergey Miniature biofuel cell as a potential power source for glucose- sensing contact lenses. American Chemical Society. 2013; Analytical Chemistry; 13 6342-6348. ISSN: 0003-2700 DOI: http://dx.doi.org/10.1021/ac4006793

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Annual Report 2013

Bergenzaun, Lill; Öhlin, Hans; Gudmundsson, Petri; Willenheimer, Ronnie; Chew, Michelle. Mitral annular plane systolic excursion (MAPSE) in shock: a valuable echocardiographic parameter in intensive care patients: Cardiovascular Ultrasound. BioMed Central Ltd. 2013; Cardiovascular Ultrasound; 16 1-8. ISSN: 1476-7120 DOI: http://dx.doi.org/10.1186/1476-7120-11-16

Wadsäter, Maria; Barauskas, Justas; Nylander, Tommy; Tiberg, Fredrik Nonlamellar lipid liquid crystalline model surfaces for biofunctional studies. Royal Society of Chemistry. 2013; Soft Matter; 37 8815-8819. ISSN: 1744-683X DOI: http://dx.doi.org/10.1039/c3sm51385h

Shao, Minling; Zafar, Muhammad Nadeem; Falk, Magnus; Ludwig, Roland; Sygmund, Christoph; Peterbauer, Clemens; Guschin, Dmitrii; MacAodha, Domhnall; O’Conghaile, Peter; Leech, Donal; Toscano, Miguel; Shleev, Sergey; Schuhmann, Wolfgang; Gorton, Lo. Optimization of a membraneless glucose/oxygen enzymatic fuel cell based on a bioanode with high coulombic efficiency and current density. Wiley-VCH Verlag. 2013; ChemPhysChem; 10 2260-2269. ISSN: 1439-4235 DOI: http://dx.doi.org/10.1002/cphc.201300046

Pita, Marcos; Gutierrez-Sanchez, Cristina; Toscano, Miguel; Shleev, Sergey; De Lacey, Antonio. Oxygen biosensor based on bilirubin oxidase immobilized on a nanostructured gold electrode. Elsevier. 2013; Bioelectrochemistry; 94 69-74. ISSN: 1567-5394 DOI: http://dx.doi.org/10.1016/j.bioelechem.2013.07.001

Seghatoleslami, Sepideh; Ohlsson, Lars; Kristina, Hamberg; Carlsson, Peter; Ericson, Dan; Ljunggren, Lennart Quantitative detection of Streptococcus mutans from saliva using FTATM elute cards and real-time polymerase chain reaction. Scientific Research Publishing. 2013; American Journal of Molecular Biology; 3 148-152. ISSN: 2161-6620 DOI: http://dx.doi.org/[10.4236/ajmb.2013.33019

Ericson, Dan; Hamberg, Kristina; Bratthall, Gunilla; Sinkiewicz-Enggren, Gabriela; Ljunggren, Lennart. Salivary IgA response to probiotic bacteria and mutans streptococci after the use of chewing gum containing Lactobacillus reuteri. Wiley Blackwell. 2013; Pathogens and Disease; 3 82-87. ISSN: 2049-632X DOI: http://dx.doi.org/10.1111/2049-632X.12048

Panrkatov, Dmitry; Blum, Zoltan; Suyatin, Dmitry; Popov, Vladimir; Shleev, Sergey. Self-charging electrochemical biocapacitor. John Wiley & Sons. 2014; ChemElectroChem; 2, 343-346. ISSN: 2196-0216 DOI: http://dx.doi.org/10.1002/celc.201300142

Björklund, Sebastian; Ruzgas, Tautgirdas; Nowacka, Agnieszka; Dahi, Ihab; Topgaard, Daniel; Sparr, Emma; Engblom, Johan. Skin membrane electrical impedance properties under the influence of a varying water gradient. Elsevier. 2013; Biophysical Journal; 12 2639-2650. ISSN: 0006-3495 DOI: http://dx.doi.org/10.1016/j.bpj.2013.05.008

Mate, Diana; Garcia-Ruiz, Eva; Shubin, Vladimir; Falk, Magnus; Shleev, Sergey; Ballesteros, Antonio; Alcalde, Miguel. Switching from blue to yellow: altering the spectral properties of a high redox potential laccase by directed evolution. Informa Healthcare. 2013; Biocatalysis and Biotransformation; 1 8-21. ISSN: 1024-2422 DOI: http://dx.doi.org/10.3109/10242422.2012.749463

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Annual Report 2013

Wagner, Ricarda; Wan, Wei; Biyakal, Mustafa; Benito Pena, Elena; Moreno-Bondi, Maria; Lazraq, Issam; Sellergren, Börje; Rurack, Knut. Synthesis, spectroscopic, and analyte-responsive behavior of a polymerizable naphthalimide-based arboxylate probe and molecularly imprinted polymers prepared thereof. American Chemical Society. 2013; Journal of Organic Chemistry; 4 1377-1389. ISSN: 0022-3263 DOI: http://dx.doi.org/10.1021/jo3019522

Olsson, Gustaf; Karlsson, Björn; Schillinger, Eric; Sellergren, Börje; Nicholls, Ian. Theoretical studies of 17-beta-estradiol-imprinted prepolymerization mixtures : insights concerning the roles of cross-linking and functional monomers in template complexation and polymerization. American Chemical Society. 2013; Industrial and Engineering Chemistry Research; 39 13965-13970. ISSN: 0888-5885 DOI: http://dx.doi.org/10.1021/ie401115f

Znamenskaya, Yana; Sotres, Javier; Gavryushov, Sergei; Engblom, Johan; Arnebrant, Thomas; Kocherbitov, Vitaly. Water Sorption and Glass Transition of Pig Gastric Mucin Studied by QCM-D. American Chemical Society ACS. 2013; Journal of Physical Chemistry B; 8 2554–2563. ISSN: 1520-6106 DOI: http://dx.doi.org/10.1021/jp311968b

Sotres, J., Barrantes, A., Lindh, L., Arnebrant, T. Strategies for a Direct Characterization of Phosphoproteins on Hydroxyapatite Surfaces. Caries Research, 48: 98-110, 2013.

Barrantes, A., Arnebrant, T., Lindh, L. Characteristics of saliva films adsorbed onto dental materials studied by QCM-D. Colloids and Surfaces A: Physicochemical and Engineering Aspects, 2013 DOI: http://dx.doi.org/10.1016/j.colsurfa.2013.05.054

Cecchinato F, Xue Y, Karlsson J, He W, Wennerberg A, Mustafa K, Andersson M, Jimbo R. In vitro evaluation of human fetal osteoblast response to magnesium loaded mesoporous TiO2 coating. J Biomed Mater Res A. 2013 Dec 12. doi: 10.1002/jbm.a.35062. [Epub ahead of print]

Ostman PO, Wennerberg A, Ekestubbe A, Albrektsson T. Immediate Occlusal Loading of NanoTite™ Tapered Implants: A Prospective 1-Year Clinical and Radiographic Study. Clin Implant Dent Relat Res. 2013 Dec;15 (6):809-18. doi: 10.1111/j.1708-8208.2011.00437.x. Epub 2012 Jan 17.

Jimbo R, Anchieta R, Baldassarri M, Granato R, Marin C, Teixeira HS, Tovar N,Vandeweghe S, Janal MN, Coelho PG Histomorphometry and bone mechanical property evolution around different implant systems at early healing stages: an experimental study in dogs. Implant Dent. 2013 Dec; 22(6): 596-603. doi: 10.1097/ID.0b013e31829f1f4b.

Naito Y, Terukina T, Galli S, Kozai Y, Vandeweghe S, Tagami T, Ozeki T, Ichikawa T, Coelho PG, Jimbo R. The effect of simvastatin-loaded polymeric microspheres in a critical size bone defect in the rabbit calvaria. Int J Pharm. 2013 Dec 1;461(1-2):157-162. doi: 10.1016/j.ijpharm.2013.11.046. [Epub ahead of print]

Jimbo R, Giro G, Marin C, Granato R, Suzuki M, Tovar N, Lilin T, Janal M, Coelho PG. Simplified drilling technique does not decrease dental implant osseointegration: a preliminary report. J Periodontol. 2013 Nov; 84(11): 1599-605. doi: 10.1902/jop.2012.120565. Epub 2012 Dec 5.

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Annual Report 2013

Yeniyol S, Jimbo R, Marin C, Tovar N, Janal MN, Coelho PG The effect of drilling speed on early bone healing to oral implants. Oral Surg Oral Med Oral Pathol Oral Radiol. 2013 Nov; 116(5): 550-5. doi: 10.1016/j.oooo.2013.07.001. Epub 2013 Sep 20.

Altmann B, Kohal RJ, Steinberg T, Tomakidi P, Bächle-Haas M, Wennerberg A, Att W. Distinct cell functions of osteoblasts on UV-functionalized titanium- and zirconia-based implant materials are modulated by surface topography. Tissue Eng Part C Methods. 2013 Nov; 19(11): 850-63. doi: 10.1089/ten.TEC.2012.0695. Epub 2013 Jun 4.

Galli S, Jimbo R, Andersson M, Bryington M, Albrektsson T Surface characterization and clinical review of two commercially available implants. Implant Dent. 2013 Oct; 22(5): 507-18. doi: 10.1097/ID.0b013e318294308f.

Vandeweghe S, Coelho PG, Vanhove C, Wennerberg A, Jimbo R. Utilizing micro-computed tomography to evaluate bone structure surrounding dental implants: a comparison with histomorphometry J Biomed Mater Res B Appl Biomater. 2013 Oct; 101(7): 1259-66. doi: 10.1002/jbm.b.32938. Epub 2013 May 10.

Coelho PG, Teixeira HS, Marin C, Witek L, Tovar N, Janal MN, Jimbo R. The in vivo effect of P-15 coating on early osseointegration. J Biomed Mater Res B Appl Biomater. 2013 Sep 16. doi: 10.1002/jbm.b.33020. [Epub ahead of print]

Albrektsson T, Dahlin C, Jemt T, Sennerby L, Turri A, Wennerberg A . Is Marginal Bone Loss around Oral Implants the Result of a Provoked Foreign Body Reaction? Clin Implant Dent Relat Res. 2013 Sep 4. doi: 10.1111/cid.12142. [Epub ahead ofprint]

Vandeweghe S, Nicolopoulos C, Thevissen E, Jimbo R, Wennerberg A, De Bruyn H. Immediate loading of screw-retained all-ceramic crowns in immediate versus delayed single implant placement. Int J Prosthodont. 2013 Sep-Oct; 26(5):458-64. doi: 10.11607/ijp.3075.

Chowdhary R, Halldin A, Jimbo R, Wennerberg A. Influence of Micro Threads Alteration on Osseointegration and Primary Stability of Implants: An FEA and In Vivo Analysis in Rabbits. Clin Implant Dent Relat Res. 2013 Aug 27. doi: 10.1111/cid.12143. [Epub ahead of print]

Tovar N, Jimbo R, Gangolli R, Perez L, Manne L, Yoo D, Lorenzoni F, Witek L,Coelho PG. Evaluation of bone response to various anorganic bovine bone xenografts: an experimental calvaria defect study Int J Oral Maxillofac Surg. 2013 Aug 12. pii: S0901-5027(13)00298-1. doi: 10.1016/j.ijom.2013.07.005. [Epub ahead of print]

De Wilde EA, Jimbo R, Wennerberg A, Naito Y, Coucke P, Bryington MS, Vandeweghe S, De Bruyn H. The Soft Tissue Immunologic Response to Hydroxyapatite-Coated Transmucosal Implant Surfaces: A Study in Humans. Clin Implant Dent Relat Res. 2013 Aug 5. doi: 10.1111/cid.12128. [Epub ahead of print]

Hirakawa Y, Jimbo R, Shibata Y, Watanabe I, Wennerberg A, Sawase T. Accelerated bone formation on photo-induced hydrophilic titanium implants: anexperimental study in the dog mandible. Clin Oral Implants Res. 2013 Aug; 24 Suppl A100:139-44. doi: 10.1111/j.1600-0501.2011.02401.x. Epub 2012 Jan 17.

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Vandeweghe S, Leconte C, Ono D, Coelho PG, Jimbo R. Comparison of histological and three-dimensional characteristics of porous titanium granules and deproteinized bovine particulate grafts used for sinus floor augmentation in humans: a pilot study. Implant Dent. 2013 Aug; 22(4):339-43. doi: 10.1097/ID.0b013e3182938d03.

Yoo D, Tovar N, Jimbo R, Marin C, Anchieta RB, Machado LS, Montclare J, Guastaldi FP, Janal MN, Coelho PG Increased osseointegration effect of bone morphogenetic protein 2 on dental implants: An in vivo study J Biomed Mater Res A. 2013 Jul 12. doi: 10.1002/jbm.a.34862.[Epub ahead of print]

Chrcanovic BR, Martins MD, Wennerberg A. Immediate Placement of Implants into Infected Sites: A Systematic Review. Clin Implant Dent Relat Res. 2013 Jul 2. doi: 10.1111/cid.12098. [Epub ahead of print]

Jimbo R, Halldin A, Janda M, Wennerberg A, Vandeweghe S. Vertical fracture and marginal bone loss of internal-connection implants: a finite element analysis. Int J Oral Maxillofac Implants. 2013 Jul-Aug; 28(4):e171-6. doi:10.11607/jomi.3052.

Wennerberg A, Jimbo R, Stübinger S, Obrecht M, Dard M, Berner S. Nanostructures and hydrophilicity influence osseointegration: a biomechanical study in the rabbit tibia. Clin Oral Implants Res. 2013 Jun 19. doi: 10.1111/clr.12213. [Epub ahead of print]

Chowdhary R, Jimbo R, Thomsen CS, Carlsson L, Wennerberg A. The osseointegration stimulatory effect of macrogeometry-modified implants: a study in the rabbit. Clin Oral Implants Res. 2013 Jun 18. doi: 10.1111/clr.12212. [Epub ahead of print]

Jimbo R, Tovar N, Yoo DY, Janal MN, Anchieta RB, Coelho PG. The effect of different surgical drilling procedures on full laser-etched microgrooves surface-treated implants: an experimental study in sheep. Clin Oral Implants Res. 2013 Jun 17. doi: 10.1111/clr.12216. [Epub ahead ofprint]

Moura CC, Machado JR, Silva MV, Rodrigues DB, Zanetta-Barbosa D, Jimbo R, TovarN, Coelho PG. Evaluation of human polymorphonuclear behavior on textured titanium and calcium-phosphate coated surfaces.Biomed Mater. 2013 Jun; 8(3): 035010. doi: 10.1088/1748-6041/8/3/035010. Epub 2013 Apr 19.

He W, Andersson M, de Souza PP, de Souza Costa CA, Muñoz EM, Schwartz-Filho HO, Hayashi M, Hemdal A, Fredel A, Wennerberg A, Jimbo R. Osteogenesis-inducing calcium phosphate nanoparticle precursors applied to titanium surfaces. Biomed Mater. 2013 Jun; 8(3):035007. doi: 10.1088/1748-6041/8/3/035007. Epub 2013 Apr 5.

Moura CG, Souza MA, Kohal RJ, Dechichi P, Zanetta-Barbosa D, Jimbo R, Teixeira CC, Teixeira HS, Tovar N, Coelho PG. Evaluation of osteogenic cell culture and osteogenic/peripheral blood mononuclear human cell co-culture on modified titanium surfaces. Biomed Mater. 2013 Jun; 8(3):035002. doi: 10.1088/1748-6041/8/3/035002. Epub 2013 Mar 26.

Bryington MS, Hayashi M, Kozai Y, Vandeweghe S, Andersson M, Wennerberg A, Jimbo R The influence of nano hydroxyapatite coating on osseointegration after extendedhealing periods. Dent Mater. 2013 May; 29(5): 514-20. doi: 10.1016/j.dental.2013.02.004. Epub 2013 Mar 13.

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Bougas K, Jimbo R, Vandeweghe S, Hayashi M, Bryington M, Kozai Y, Schwartz-Filho HO, Tovar N, Adolfsson E, Ono D, Coelho PG, Wennerberg A. Bone apposition to laminin-1 coated implants: histologic and 3D evaluation. Int J Oral Maxillofac Surg. 2013 May; 42(5):677-82. doi: 10.1016/j.ijom.2012.11.008. Epub 2012 Dec 8.

Takeshita K, Funaki K, Jimbo R, Takahashi T.Nasopalatine duct cyst developed in association with dental implant treatment: A case report and histopathological observation. J Oral Maxillofac Pathol. 2013 May; 17(2): 319. doi: 10.4103/0973-029X.119761.

Wennerberg A, Bougas K, Jimbo R, Albrektsson T. Implant coatings: new modalities for increased osseointegration. Am J Dent. 2013 Apr; 26 (2): 105-12.

Meirelles L, Uzumaki ET, Lima JH, Muller CA, Albrektsson T, Wennerberg A, Lambert CS. A novel technique for tailored surface modification of dental implants – a step wise approach based on plasma immersion ion implantation. Clin Oral Implants Res. 2013 Apr; 24 (4): 461-7. doi: 10.1111/j.1600-0501.2011.02352.x. Epub 2011 Dec 15.

Anchieta RB, Baldassarri M, Guastaldi F, Tovar N, Janal MN, Gottlow J, Dard M, Jimbo R, Coelho PG. Mechanical Property Assessment of Bone Healing around a Titanium-Zirconium Alloy Dental Implant. Clin Implant Dent Relat Res. 2013 Mar 25. doi: 10.1111/cid.12061. [Epub ahead of print]

Chowdhary R, Jimbo R, Thomsen C, Carlsson L, Wennerberg A.Biomechanical evaluation of macro and micro designed screw-type implants: an insertion torque and removal torque study in rabbits Clin Oral Implants Res. 2013 Mar; 24 (3): 342-6. doi: 10.1111/j.1600-

Rosa MB, Albrektsson T, Francischone CE, Filho HO, Wennerberg A. Micrometric characterization of the implant surfaces from the five largest companies in Brazil, the second largest worldwide implant market. Int J Oral Maxillofac Implants. 2013 Mar-Apr; 28 (2): 358-65. doi: 10.11607/jomi.2791.

Hayashi M, Jimbo R, Xue Y, Mustafa K, Andersson M, Wennerberg A. Photocatalytically induced hydrophilicity influences bone remodelling at longer healing periods: a rabbit study. Clin Oral Implants Res. 2013 Feb 26. doi: 10.1111/clr.12138. [Epub ahead of print]

Odatsu T, Jimbo R, Wennerberg A, Watanabe I, Sawase T Effect of polishing and finishing procedures on the surface integrity of restorative ceramics. Am J Dent. 2013 Feb; 26 (1): 51-5.

Wennerberg A, Svanborg LM, Berner S, Andersson M. Spontaneously formed nanostructures on titanium surfaces. Clin Oral Implants Res. 2013 Feb; 24 (2): 203-9. doi: 10.1111/j.1600-0501.2012.02429.x. Epub 2012 Feb 15.

Valverde GB, Jimbo R, Teixeira HS, Bonfante EA, Janal MN, Coelho PG. Evaluation of surface roughness as a function of multiple blasting processing variables. Clin Oral Implants Res. 2013 Feb; 24 (2): 238-42. doi: 10.1111/j.1600-0501.2011.02392.x. Epub 2011 Dec 20.

Chowdhary R, Halldin A, Jimbo R, Wennerberg A.Evaluation of stress pattern generated through various thread designs of dental implants loaded in a condition of immediately after placement and on osseointegration--an FEA study. Implant Dent. 2013 Feb; 22 (1): 91-6. doi: 10.1097/ID.0b013e31827daf55.

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Barkarmo S, Wennerberg A, Hoffman M, Kjellin P, Breding K, Handa P, Stenport V. Nano-hydroxyapatite-coated PEEK implants: a pilot study in rabbit bone. J Biomed Mater Res A. 2013 Feb; 101(2): 465-71. doi: 10.1002/jbm.a.34358. Epub 2012 Aug 3.

Bougas K, Jimbo R, Vandeweghe S, Tovar N, Baldassarri M, Alenezi A, Janal M, Coelho PG, Wennerberg In Vivo Evaluation of a Novel Implant Coating Agent: Laminin-1. A Clin Implant Dent Relat Res. 2013 Jan 11. doi: 10.1111/cid.12037. [Epub ahead of print]

Browaeys H, Vandeweghe S, Johansson CB, Jimbo R, Deschepper E, De Bruyn H. The histological evaluation of osseointegration of surface enhanced microimplants immediately loaded in conjunction with sinuslifting in humans. Clin Oral Implants Res. 2013 Jan; 24 (1): 36-44. doi:10.1111/j.1600-0501.2011.02398.x. Epub 2012 Jan 6.

Giro G, Tovar N, Marin C, Bonfante EA, Jimbo R, Suzuki M, Janal MN, Coelho PG.The effect of simplifying dental implant drilling sequence on osseointegration: an experimental study in dogs. Int J Biomater. 2013;2013:230310. doi: 10.1155/2013/230310. Epub 2013 Jan 30.

Guastaldi FP, Yoo D, Marin C, Jimbo R, Tovar N, Zanetta-Barbosa D, Coelho PG. Plasma treatment maintains surface energy of the implant surface and enhances osseointegration. Int J Biomater. 2013;2013:354125. doi: 10.1155/2013/354125. Epub 2013 Jan 10.

Marin C, Jimbo R, Lorenzoni FC, Witek L, Teixeira H, Bonfante E, Gil J, Granato R, Tovar N, Coelho PG. Bone-Forming Capabilities of a Newly Developed NanoHA Composite Alloplast Infused with Collagen: A Pilot Study in the Sheep Mandible. Int J Dent. 2013;2013:296391. doi: 10.1155/2013/296391. Epub 2013 Oct 27.

Alenezi A, Naito Y, Andersson M, Chrcanovic BR, Wennerberg A, Jimbo R. Characteristics of 2 Different Commercially Available Implants with or without Nanotopography. Int J Dent. 2013;2013:769768. doi: 10.1155/2013/769768. Epub 2013 Oct 2.

Bonfante EA, Granato R, Marin C, Jimbo R, Giro G, Suzuki M, Coelho PG.Biomechanical testing of microblasted, acid-etched/microblasted, anodized, and discrete crystalline deposition surfaces: an experimental study in beagle dogs.Int J Oral Maxillofac Implants. 2013 Jan-Feb; 28(1):136-42. doi: 10.11607/jomi.2331.

Malekzadeh B, Tengvall P, Ohrnell LO, Wennerberg A, Westerlund A. Effects of locally administered insulin on bone formation in non-diabetic rats. J Biomed Mater Res A. 2013 Jan; 101(1):132-7. doi: 10.1002/jbm.a.34313. Epub 2012 Jul 24.

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CONTRIBUTIONS TO AND pARTICIpATION IN CONFERENCES AND wORkSHOpS

Ann Wennerberg. “Biomechanical, histomorphometrical and gene expression analyses used to evaluate the osseointegration process” IADR, Seattle, USA March 20-23 2013. Invited lecture

Ann Wennerberg. “Designing for life: Today and in the future” Nobel Biocare Global Symposium 2013. June 20–22 New York USA. Invited lecture

Ann Wennerberg. “Dental Implant surfaces: possibilities and threats” IN 2013 Latin American Osseointegration Congress. Sap Paulo Brazil September 25th to 28th 2013. Invited lecture

Ann Wennerberg. “Why and when do implant fail. Future challenges in prosthodontics rehabilitation of oral function” CORE October 23–25, Beijing, China 2013. Invited lecture

Ann Wennerberg. “Reasons for failure of oral implants” George Zarb clinical research lecture. Deans lecture series. Toronto University, Toronto, Canada November 25, 2013. Invited lecture

Ann Wennerberg. “On macro-, micro-, and nanoaspects of implant surfaces and their importance for osseointegration” 5th International Conference on Mechanics of Biomaterials and Tissues. Sitges, Spain 8–12 December 2013. Invited lecture

Bertil Kinnby. “PAI-2 inhibits bacterial proteases” XIV International Workshop on Molecular and Cellular Biology of Plasminogen Activation, Notre Dame, IN, USA. Håkan Eriksson. “Aluminium adjuvant-induced mitochondrial alterations, Tenth Keele Meeting on Aluminium”, Winchester, UK.

Börje Sellergren. “Molecularly imprinted polymers for environmental technologies”. 11th international conference on Materials Chemistry. Warwick, UK, July 2013. Keynote speaker

Börje Sellergren. “Molecularly imprinted polymers in biomarker analysis” Biomarker workshop, HS, October 2013.

Böje Sellergren. “Designing MIPs for food safety applications: endocrine disruptors, antibi-otics, dioxins “. Sino German conference on Food Safety, Beijng, September, 2013.

Gunnel Svensäter. “Infection control on the biofilm level” University of Minneapolis, MN, USA. Invited speaker

Johan Engblom. “A Water Gradient can be used to Regulate Biophysical Properties of Skin and Mucosa”. The Biennial Australian Colloidal and Interface Symposium, Australia 3–7/2 2013.

oRAl pResentAtions 2013

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Annual Report 2013

Johan Engblom. “A Water Gradient can be used to Regulate Biophysical Properties of Skin and Mucosa”. Applied Mathematics , ANU, Canberra Australia 18/2 2013

Albèr C. Prospects in controlled uptake, delivery and release Symposium SP, Stockholm 23/10 2013.

Liselott Lindh, Alejandro Barrantes, Thomas Arnebrant. “Characteristics of saliva films adsorbed onto different dental materials” IADR, Seattle

Sergey Shleev. Invited Lecture entitled: “On the possibility of uphill intramolecular electron transfer in multicopper oxidases” at the mini-symposium on Bioelectrochemical systems (BES), February 01, 2013, Lund, Sweden.

Sergey Shleev. Invited Lecture entitled: “Recent advances in biofuel cells and biobatteries” at the 10th International Electrochemistry Days, September 05, 2013, Konya, Turkey.

Sergey Shleev. Invited Lecture entitled: “Teardrop analysis” at the World Diabetes Day, November 09, 2013, Malmö, Sweden.

Sergey Shleev. Tutorial Lecture entitled: “Recent advances in biofuel cells” at the 6th International Workshop SMCBS ”Surface Modification for Chemical and Biochemical Sensing”, November 11, 2013, Lochow, Poland

Vitaly Kocherbitov. “Application of Humidity-Scanning QCM-D Method for Surfactant Phase Studies” ECIS2013, Sofia, Bulgaria.

Vitaly Kocherbitov. “Hydration-induced phase and glass transitions in soft matter films revealed by QCM-D” COST workshop The balance of interactions in soft-matter systems, Nicosia, Cyprus. Invited speaker

contAct infoRmAtionContact Information


Malmö University

Faculty of Health and Society

205 06 Malmö

Sweden

Center director: Doctor Anna Holmberg

Tel: +46 40 665 79 35

e-mail: [email protected]

www.mah.se/biofilms

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