biodevice technology, ltd. compact potentiostat...
TRANSCRIPT
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High Sensitive ImmunochromatographyPCT/JP2006/323617
DEP-Chip Series for Electrochemical Biosensors
Gold Linked Electrochemical Immuno Assay GLEIA, PCT/JP2007/56992)
R&D of Biosensors
Compact potentiostat BDTminiSTAT100
Compact electrochemical luminescence device BDTeCL-P100
BioDevice Technology, Ltd.2-13-106 Asahidai, Nomi, Ishikawa, 923-1211, JAPAN
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Products and Service ListItem Name
Printed Electrodes for Biosensors DEP-Chip seriesSP-N, SP-PEP-N, EP-P, EP-PPSR-N, SR-PER-N, ER-P, ER-PPTG-1, TG-3PCR-P01, PCR-P03
Compact Potentiostat BDTminiSTAT100BDTminiSTAT400 (4 channel)BDTminiSTATSR-6 (6 channel)BDTminiSTAT100-BTR(blue tooth connection)
Electrochemical Luminescence Device BDTeCL-P100Trial Manufacture of ImmunochromatographyManufacture of customized printed electrodes on assignmentContract research on biosensors
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Five measurement modes of CA, CV, LSV, DPV, SWV
Compact, Light weight and Easy to carry Power supplied with USB connection to
PC Easy to use with the proprietary software Optional software for Automatic peak
detection and determination
Compact Potentiostat BDTminiSTAT100
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Wireless Potentiostat BDTminiSTAT100-BTR
Five measurement modes of CA, CV, LSV, DPV, SWV
Compact, Light weight (120 g) and Easy to carry
Power supplied by two AAA size batteries
Easy to use with the proprietary software
Wireless connection to computer by Bluetooth
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Compact Multi-channel PotentiostatBDTminiSTAT400, BDTminiSTATSR-6
4
5 measurement modes of CV, LSV, CA, DPV, SWV Compact size: 130 x 100 x 40 (mm) 300 g The applied electromotive force: -2.0 +2.0 V, resolution: 2 mV The measurement range: 6 range1 nA 100 A)resolution:
0.1% Easily input measuring conditions (measurement mode, voltage
range, time and scan rate, etc.) On-line graphical representation of measurements Automatic safe-keeping of measured data (CSV file)
BDTminiSTATSR-6 Mail to [email protected]
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SystemBDTeCL-P100
Electrochemical Luminescence Device BDTeCL-P100
Compact ECL device
Simple and rapid measurement enabled by its dedicated software.
Highly sensitive and selective measurement enabled
BDTeCL-P100PC OS Windows Vista, 7, 8 (32 bit)
Size (mm)/weight (Kg) 115(L) x 125(W) x 185(H)/ 4
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SP-N, SP-P(carbon)
SR-N(gold)
Square working electrode
PCR-P01
EP-N(carbon)EP-PP(carbon, WE with ring and dam)ER-N(gold)
Round working electrode
One drop test
Adapted size for PCR tubemass production/disposal/low cost
Possible customized fabrication
Printed Electrodes DEP-Chip series
For PCRPCR-P01(carbon)PCR-P03(gold)
Large sizeTG-1 (carbon,WE with ring )
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Advantage of DEP-Chips *DEP-Chip series is a trade name of our original printedelectrodeswhich were developed for fabrication of various biosensors.
Disposable No contamination Easy to use Mass productive and low cost High quality CV < 5% Small amount of reagents and samples 1-2 L Various applications for electrochemical determination
with BDTminiSTATs and for electrochemical luminescence determination with BDTeCL-P100 Especially suitable for biosensors
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K.Kerman,Chem. Commun., 2007, 3829Detection of HIV-1 protease inhibition
Printed electrode in RT-PCR flow chip for Flu virus detection
Biosensor printed electrodes DEP-Chip series are evaluated worldwide
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Application examples of BDTminiSTAT100 and DEP-Chips
1. Total viable bacterial sensor2. DNA sensor3. Immunochemical sensor4. Enzyme sensor5. Heavy metal sensor6. Residual agricultural chemical
sensor
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Count of viable bacteria numbers
Respiratory activity consumption of oxygen
Determination of dissolved oxygen decrease by the electrochemical method
-7000
-6000
-5000
-4000
-3000
-2000
-1000
0
1000
-1400 -1200 -1000 -800 -600 -400 -200
( )
CVCyclic Voltammetry 50mV/secPrinted carbon electrode DEP-Chip SP-P
Potential mV vs. Ag/AgCl)
current(nA)
DEP-Chips: Low cost/Easy to useminiSTAT100: compact and light weight
The smallest potentiostat in the ld
2011/9/10
Reduction current of dissolved oxygen
Addition of oxygen scavenger
Total viable bacterial sensor
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Original principle of our DNA sensorJapanese patent No. 364257
100nm 100nm
A B
(Low concentration of DNAor Before amplification)
(High concentration of DNAor After amplification)
This method is suitable for detection of DNA amplification after PCR or LAMP.
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ProcedureRT-LAMP)Analyst 2011, DOI: 0.1039/c1an15638a
Primer mixture + Metylene Blue+ influenza virus + dried enzyme + buffer reagents(Loopamp ) total volume 50 L
Printed electrode was inserted in the micro tube and the tube was incubated at 63for 40 min.
During incubation, electrochemical measurements(SWV) were performed every 5 min.
An example of DNA sensor system
(A) Photograph of the screen-printed electrodes. a: screen-printed electrode, b: screen-printed electrode inserted into the 200 mL micro tube. (B)Illustration of the semi-real time monitoring system using the screen-printed electrode with a USB powered portable potentiostat. a: heat block, b:screen-printed electrode inserted into the micro tube, c: USB powered portable potentiostat, d: laptop computer.(C) Photographs of the USB powered
portable potentiostat and screen-printed electrode inserted into the micro tube with heating unit. a: USB powered portable potentiostat (BDTmini-STAT100, Bio device technology Co., Ltd., Japan).
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Example of detection of the food poisoning infectious organism gene
0
0.2
0.4
0.6
0.8
1
0.2 0.4 0.6 0.8
Oxid
ation c
urr
ent
[A
]
Potential [V]
No.1
No.2 Ipo
Ipa
decrease
Electrochemical determinationnegative
positive With salmonellas
Without salmonellas
Graph4
-0.047-0.033
-0.022-0.015
-0.014-0.01
-0.011-0.008
-0.009-0.006
-0.007-0.005
-0.006-0.004
-0.005-0.002
-0.004-0.002
-0.003-0.002
-0.003-0.002
-0.003-0.001
-0.0020
-0.0020
-0.0010
-0.0010
-0.0010.001
00.001
00.001
00.001
00.001
00.002
00.002
00.002
00.002
0.0010.002
0.0010.003
0.0010.003
0.0010.002
0.0010.002
0.0010.003
0.0020.003
0.0010.003
0.0020.004
0.0020.003
0.0020.004
0.0020.004
0.0020.003
0.0020.004
0.0020.004
0.0020.004
0.0020.004
0.0020.004
0.0020.004
0.0020.004
0.0030.004
0.0020.004
0.0030.004
0.0020.005
0.0020.005
0.0030.005
0.0030.005
0.0030.005
0.0030.005
0.0040.005
0.0030.005
0.0030.004
0.0030.005
0.0030.005
0.0030.005
0.0040.005
0.0040.005
0.0040.005
0.0040.006
0.0030.005
0.0040.005
0.0040.006
0.0040.006
0.0040.005
0.0040.006
0.0040.006
0.0040.006
0.0040.006
0.0040.006
0.0040.006
0.0040.006
0.0050.007
0.0050.007
0.0050.007
0.0050.006
0.0050.007
0.0050.007
0.0050.007
0.0060.007
0.0060.007
0.0060.007
0.0060.008
0.0060.007
0.0060.007
0.0070.007
0.0070.007
0.0070.008
0.0070.008
0.0070.008
0.0080.008
0.0080.009
0.0080.008
0.0090.01
0.0090.01
0.010.009
0.0110.01
0.0120.009
0.0120.011
0.0130.011
0.0140.011
0.0160.011
0.0170.012
0.0190.012
0.0210.013
0.0240.013
0.0260.014
0.030.015
0.0340.017
0.0370.018
0.0420.019
0.0460.021
0.0520.023
0.0570.024
0.0620.028
0.0670.031
0.0730.035
0.0780.039
0.0830.043
0.0880.048
0.0910.053
0.0930.058
0.0960.065
0.0980.07
0.0990.076
0.1070.087
0.1070.091
0.1080.097
0.110.101
0.1130.105
0.1170.108
0.1230.11
0.1310.11
0.1460.112
0.1630.111
0.1850.111
0.2110.111
0.2370.114
0.2640.117
0.2880.121
0.3160.127
0.3440.134
0.3730.143
0.40.152
0.430.163
0.4620.176
0.4920.187
0.5220.198
0.5530.206
0.5840.209
0.6150.209
0.6420.207
0.6690.202
0.690.195
0.7060.19
0.7170.186
0.7150.18
0.7350.186
0.720.181
0.70.177
0.6750.175
0.6490.172
0.6240.169
0.6030.166
0.5830.166
0.5660.163
0.5480.16
0.5340.161
0.5220.158
0.510.158
0.4990.158
0.490.156
0.480.155
0.4710.156
0.4630.156
0.4560.156
0.4480.157
0.4410.157
0.4350.159
0.4280.16
0.4230.161
0.4170.163
0.4120.164
0.4070.166
0.4010.169
0.3980.17
0.3920.172
0.3890.175
0.3850.177
0.3910.186
0.3870.189
0.3830.193
0.3810.197
0.380.202
0.3770.208
0.3780.212
0.3770.218
0.3780.224
0.3790.231
0.380.239
0.3820.247
0.3850.257
0.3890.265
0.3930.276
0.3950.285
0.4020.297
0.4070.307
0.4120.32
0.4170.334
0.4220.348
0.4290.363
0.4390.378
0.4470.392
0.4540.41
0.4630.429
0.4710.446
0.4810.469
0.4920.488
0.5010.511
0.5120.534
0.520.555
0.4870.531
0.5020.555
0.5160.582
0.5290.608
0.5420.637
0.5570.665
0.5680.696
0.5840.726
0.60.76
0.6160.792
0.6310.828
0.6490.866
0.6650.902
0.6880.94
0.7060.98
0.7251.023
0.7491.071
0.7711.118
0.7911.167
0.8161.212
0.8391.266
0.8661.321
0.8921.328
0.921.328
0.951.328
0.981.328
No.1
No.2
Potential [V]
Oxidation current [A]
Sheet1
[V] No.1No.1No.3No.2
0-0.025-0.047-0.008-0.033
0.004-0.011-0.022-0.003-0.015
0.008-0.007-0.014-0.002-0.01
0.012-0.005-0.011-0.001-0.008
0.016-0.003-0.0090-0.006
0.02-0.003-0.007-0.001-0.005
0.024-0.002-0.0060-0.004
0.028-0.002-0.0050.001-0.002
0.032-0.002-0.0040.001-0.002
0.036-0.001-0.0030.001-0.002
0.040-0.0030.001-0.002
0.0440-0.0030.001-0.001
0.0480-0.0020.0010
0.0520-0.0020.0020
0.0560-0.0010.0020
0.060.001-0.0010.0020
0.0640-0.0010.0020.001
0.0680.00100.0020.001
0.0720.00100.0030.001
0.0760.00100.0030.001
0.080.00100.0030.001
0.0840.00100.0030.002
0.0880.00200.0030.002
0.0920.00200.0030.002
0.0960.00200.0040.002
0.10.0020.0010.0040.002
0.1040.0020.0010.0040.003
0.1080.0020.0010.0050.003
0.1120.0020.0010.0050.002
0.1160.0020.0010.0050.002
0.120.0020.0010.0050.003
0.1240.0020.0020.0050.003
0.1280.0020.0010.0050.003
0.1320.0030.0020.0060.004
0.1360.0030.0020.0060.003
0.140.0030.0020.0050.004
0.1440.0030.0020.0060.004
0.1480.0030.0020.0050.003
0.1520.0030.0020.0060.004
0.1560.0030.0020.0060.004
0.160.0030.0020.0060.004
0.1640.0030.0020.0060.004
0.1680.0030.0020.0060.004
0.1720.0030.0020.0060.004
0.1760.0030.0020.0070.004
0.180.0030.0030.0070.004
0.1840.0030.0020.0070.004
0.1880.0040.0030.0070.004
0.1920.0030.0020.0070.005
0.1960.0040.0020.0070.005
0.20.0030.0030.0070.005
0.2040.0040.0030.0070.005
0.2080.0040.0030.0070.005
0.2120.0040.0030.0060.005
0.2160.0040.0040.0070.005
0.220.0040.0030.0070.005
0.2240.0040.0030.0080.004
0.2280.0040.0030.0070.005
0.2320.0040.0030.0080.005
0.2360.0040.0030.0080.005
0.240.0040.0040.0080.005
0.2440.0040.0040.0070.005
0.2480.0040.0040.0070.005
0.2520.0040.0040.0070.006
0.2560.0040.0030.0080.005
0.260.0050.0040.0080.005
0.2640.0050.0040.0080.006
0.2680.0050.0040.0090.006
0.2720.0050.0040.0080.005
0.2760.0050.0040.0090.006
0.280.0050.0040.0090.006
0.2840.0050.0040.0090.006
0.2880.0050.0040.0090.006
0.2920.0050.0040.0110.006
0.2960.0050.0040.0090.006
0.30.0060.0040.0090.006
0.3040.0050.0050.0090.007
0.3080.0060.0050.0090.007
0.3120.0050.0050.0090.007
0.3160.0060.0050.0090.006
0.320.0060.0050.010.007
0.3240.0060.0050.0090.007
0.3280.0070.0050.0090.007
0.3320.0060.0060.0090.007
0.3360.0070.0060.0080.007
0.340.0070.0060.010.007
0.3440.0070.0060.0090.008
0.3480.0080.0060.010.007
0.3520.0070.0060.010.007
0.3560.0070.0070.010.007
0.360.0080.0070.010.007
0.3640.0080.0070.010.008
0.3680.0080.0070.010.008
0.3720.0080.0070.010.008
0.3760.0080.0080.0110.008
0.380.0080.0080.0110.009
0.3840.0080.0080.0110.008
0.3880.010.0090.0110.01
0.3920.0090.0090.0110.01
0.3960.010.010.0110.009
0.40.010.0110.0110.01
0.4040.0110.0120.0120.009
0.4080.010.0120.0120.011
0.4120.0110.0130.0120.011
0.4160.0110.0140.0130.011
0.420.0120.0160.0130.011
0.4240.0120.0170.0140.012
0.4280.0120.0190.0130.012
0.4320.0140.0210.0150.013
0.4360.0130.0240.0140.013
0.440.0140.0260.0150.014
0.4440.0170.030.0170.015
0.4480.0160.0340.0170.017
0.4520.0180.0370.0180.018
0.4560.0190.0420.020.019
0.460.0220.0460.0220.021
0.4640.0230.0520.0220.023
0.4680.0260.0570.0250.024
0.4720.0290.0620.0270.028
0.4760.0310.0670.0290.031
0.480.0350.0730.0330.035
0.4840.0390.0780.0350.039
0.4880.0420.0830.0380.043
0.4920.0470.0880.0430.048
0.4960.0510.0910.0440.053
0.50.0550.0930.0490.058
0.5040.0590.0960.0520.065
0.5080.0620.0980.0540.07
0.5120.0660.0990.0570.076
0.5160.0740.1070.0650.087
0.520.0770.1070.0670.091
0.5240.0790.1080.0680.097
0.5280.0810.110.0680.101
0.5320.0820.1130.0690.105
0.5360.0840.1170.070.108
0.540.0870.1230.0710.11
0.5440.0880.1310.0710.11
0.5480.0920.1460.0710.112
0.5520.0970.1630.0710.111
0.5560.1050.1850.0720.111
0.560.1190.2110.0740.111
0.5640.1330.2370.0750.114
0.5680.1530.2640.0770.117
0.5720.1740.2880.0810.121
0.5760.1990.3160.0860.127
0.580.2260.3440.0920.134
0.5840.2520.3730.0980.143
0.5880.2820.40.1060.152
0.5920.3120.430.1150.163
0.5960.3420.4620.1210.176
0.60.3720.4920.130.187
0.6040.4020.5220.1340.198
0.6080.4340.5530.140.206
0.6120.4660.5840.1420.209
0.6160.4980.6150.1440.209
0.620.5320.6420.1440.207
0.6240.5640.6690.1440.202
0.6280.5940.690.1430.195
0.6320.6220.7060.1410.19
0.6360.650.7170.140.186
0.640.6720.7150.1380.18
0.6440.720.7350.1430.186
0.6480.7370.720.1410.181
0.6520.7470.70.140.177
0.6560.7540.6750.1390.175
0.660.750.6490.1390.172
0.6640.7420.6240.1380.169
0.6680.7280.6030.1380.166
0.6720.7080.5830.1370.166
0.6760.6870.5660.1360.163
0.680.6610.5480.1360.16
0.6840.6360.5340.1360.161
0.6880.6120.5220.1360.158
0.6920.5910.510.1330.158
0.6960.570.4990.1350.158
0.70.550.490.1350.156
0.7040.5350.480.1330.155
0.7080.5230.4710.1320.156
0.7120.5010.4630.1330.156
0.7160.4850.4560.1320.156
0.720.4730.4480.1340.157
0.7240.4620.4410.1340.157
0.7280.4520.4350.1350.159
0.7320.4420.4280.1340.16
0.7360.4340.4230.1350.161
0.740.4260.4170.1360.163
0.7440.4170.4120.1370.164
0.7480.410.4070.1390.166
0.7520.4040.4010.1380.169
0.7560.3980.3980.140.17
0.760.3910.3920.1410.172
0.7640.3840.3890.1440.175
0.7680.3770.3850.1440.177
0.7720.3840.3910.1490.186
0.7760.3770.3870.1510.189
0.780.3720.3830.1540.193
0.7840.3690.3810.1550.197
0.7880.3640.380.1580.202
0.7920.360.3770.1610.208
0.7960.3560.3780.1640.212
0.80.3530.3770.1670.218
0.8040.3520.3780.170.224
0.8080.350.3790.1740.231
0.8120.3490.380.1780.239
0.8160.3480.3820.1830.247
0.820.3480.3850.1880.257
0.8240.3480.3890.1920.265
0.8280.350.3930.1970.276
0.8320.3470.3950.2030.285
0.8360.3510.4020.2080.297
0.840.3520.4070.2160.307
0.8440.3540.4120.2210.32
0.8480.3560.4170.2290.334
0.8520.360.4220.2360.348
0.8560.3620.4290.2450.363
0.860.3670.4390.2530.378
0.8640.3720.4470.2610.392
0.8680.3790.4540.270.41
0.8720.3820.4630.280.429
0.8760.3880.4710.2910.446
0.880.3910.4810.3010.469
0.8840.3980.4920.3130.488
0.8880.4020.5010.3240.511
0.8920.4060.5120.3360.534
0.8960.4130.520.3470.555
0.90.3840.4870.3260.531
0.9040.3970.5020.3410.555
0.9080.4050.5160.3540.582
0.9120.4130.5290.370.608
0.9160.4220.5420.3830.637
0.920.4320.5570.3990.665
0.9240.4410.5680.4140.696
0.9280.4520.5840.4330.726
0.9320.4610.60.4510.76
0.9360.4730.6160.4690.792
0.940.4830.6310.4890.828
0.9440.4940.6490.5110.866
0.9480.5070.6650.5310.902
0.9520.5210.6880.5530.94
0.9560.5320.7060.5750.98
0.960.5470.7250.6011.023
0.9640.5630.7490.6271.071
0.9680.5780.7710.6541.118
0.9720.5930.7910.6811.167
0.9760.6090.8160.7091.212
0.980.6290.8390.741.266
0.9840.6430.8660.7721.321
0.9880.660.8920.8051.328
0.9920.6810.920.8441.328
0.9960.7030.950.8751.328
10.7250.980.9171.328
Sheet1
No.1
No.2
[V]
[A]
Sheet2
Sheet3
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Application of DNA sensor
1. Pathogenic microbeSalmonella, E. coli O-157, MRSA(nosocomial infection,Periodontal disease bacteria, Anthrax
2. Infectious virusInfluenza, Hepatitis virus B
3. FoodDetermination of species of meatpork, beef, chicken,genetically modified food GMO corn, GMO soybean etc.
4. SNPsdrug resistant gene, Alzheimers disease-associated gene
-
1.2V, 40secin 0.1N HCl
DPV*from 1V to 0V
Gold-Linked Electrochemical Immuno-Assay(GLEIA, PCT/JP2007/56992, JP5187759)
Gold nanoparticle redox signal enhancement for detection of antigensAfter the recognition reaction between the surface-immobilized primary antibody and hCG, the captured antigen was sandwiched with a secondary antibody that was labeled with gold-nanoparticles. Then the amount ofgold nanoparticles was determined by its redox signal.
The primary antibody wasImmobilized directly on the workingelectrode, and a sandwich-typeimmuno-reaction was performed
Reference
A high potential was applied In HCl for the oxidation of gold nanoparticles
DPV* : differential pulse voltammetry
The voltammetric measurement was performed.
Direct fabrication of catalytic metal nanoparticles onto the surface of a screen-printed carbon electrode, Electrochemistry Communications 8, p1375-1380 (2006).
Gold nanoparticle-based redox signal enhancement for sensitive detection of human chorionic gonadotropin hormone,Koutarou Idegami, Miyuki Chikae, Kagan Kerman, Naoki Nagatani, Teruko Yuhi, Taturo Endo, and Eiichi Tamiya, Electroanalysis 20, p14-21 (2008)
-
Comparative table of GLEIA and ELISA, CLEIAGLEIA ELISA, CLEIA
Labeling Gold nanoparticle Enzyme
Detection method
Electrochemical measurementreduction current of gold particle
Optical measurementcoloring or fluorescence by enzyme
activity
Measurement time
Within 60 min(within 15 min by combination with micro
flow channels)
2 3 hours
threshold pg/mL ~ ng/mL pg/mL ~ ng/mL
Sample volume
1 L A few tens of L
cost Less than 800JPY/test 8001600JPY/testdevice inexpensivecompact expensivelarge sizedpurpose Medical/foods/environmental
For point of care testing at bed-side, clinic,manufacturing floor)
Medical/foods/environmentalFor facilities for medical examination,
Laboratories, stationary/batch processing
Application examined
hCG, insulin in human serum, IgA, hemoglobin, albumin in human urine, DNA detection after PCR with FITC and biotin
labeled primer
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Application ofGold-Linked Electrochemical Immuno-Assay I
Sample vlume:1 1.5LLower detection limit: 62.5 pg/mL
Human Chorionic Gonadotropin Hormone standard curves constructed with buffer () and with serum ()
Cur
rent
(A)
0 62.5 100 10000.0
0.2
0.4
0.6
0.8
1.0
1.2
1.4
1.6
1.8
2.0
hCG concn. (pg/mL)
control1 R2=0.9996
serum R2=0.9971
Cur
rent
(A)
Potential (V)
0pg/ml
62.5pg/ml
125pg/ml
250pg/ml
500pg/ml
1000pg/m
Typical differential pulse voltammograms of GLEIA.
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Glucose sensor with Glucose Dehydrogenase using DEP-Chips (Chrono-amperometry)
Glucose 100 mg/dL
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DEP-Chip SP-N SPKI13Dilution with 0.1N
Pb conc (ppb) Peak Height (A)0 0.15010 0.385100 0.624
Heavy metal determinationDPV)
Pb
Cd
DEP-Chip SR-N SRJR10Dilution with 0.1N
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Detection of residual agricultural chemicals (RAC) using inhibition of Acetylcholine esterase activityElectrochemical method
Acetylcholine esterase (AChE) mediates the following reaction.Agricultural chemicals inhibit the activity of AChE and inhibit formation of thiocholine (TCh)
CH3)3 N+CH2CH2SCOCH3 + H2O CH3)3 N+CH2CH2SH + CH3COOHATCh TCh
Principle of reactionAChE
CH3)3 N+CH2CH2SCOCH3 + H2OATCh
AChE RAC
AChE converts ATCh to TCh. After 10 min reaction, the current became larger by the increased TCh. When the activity of AChEwas inhibited by RAC, TChwas not formed and the increase of the current was also inhibited.without RAC with RAC (chloropyrifos 5 ppm)
Blue line: 2 min after ACTh addition, Red line: 10 min after ACTh addition
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Article list using DEP-ChipsDirect fabrication of catalytic metal nanoparticles onto the surface of a screen-printed carbon electrode.M. Chikae, K. Idegami, K. Kerman, N. Nagatani, M. Ishikawa, Y. Takamura, E. TamiyaElectrochemistry Communications, 8, 1375, 2006
An electrochemical on-field sensor system for the detection of compost maturityM. Chikae, K. Kerman, N. Nagatani, Y. Takamura, E. TamiyaAnalytica Chimica Acta, 581, 364, 2006
Label-free electrical sensing of small-molecule inhibition on tyrosine phosphorylationK. Kerman, M. Vestergaard, E. TamiyaAnal. Chem., 79, 6881, 2007
Electrochemical DNA biosensor using a disposable electrochemical printed (DEP) chip for the detection of SNPs from unpurified PCR ampliconsM. U. Ahmed, K. Idegami, M. Chikae, K. Kerman, P. Chaumpluk, S. Yamamura, E. TamiyaAnalyst, 132, 431, 2007
Gold Nanoparticle-Based Redox Signal Enhancement for Sensitive Detection of Human Chorionic Gonadotropin HormoneK. Idegami, M. Chikae, K. Kerman, N. Nagatani, T. Yuhi, T. Enco, E. TamiyaElectroanalysis, 20, 14, 2008
Electrochemical Biosensors for Medical and Food ApplicationsM. U. Ahmed, M. M. Hossain, E. TamiyaElectroanalysis, 20, 616, 2008
Level-free Electrochemical Detection for Food Allergen using Screen Printed Carbon ElectrodeMasato SAITO, Masaaki KITSUNAI, Mihnaz Uddin AHMED, Shigeru SUGIYAMA, and Eiichi TAMIYA, Electrochemistry, 76(8), 606-609, 2008
Electrochemical genosensor for the rapid detection of GMO using loop-mediated isothermal amplificationM. U. Agnedm M.Saito, M. M. Hossain, S. R.Rao, S. Furui, A. Hino, Y. Takamura, M. Takagi, E.TamiyaAnalyst, 134, 966, 2009
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Article list using DEP-Chips IIRapid, Sensitive, and Label-Free Impedimetric Detection of a Single-Nucleotide Polymorphism Correlated to Kidney DiseaseAlessandra Bonanni, Martin Pumera, and Yuji MiyaharaAnal. Chem. 2010, 82, 37723779
Meat species identification based on the loop mediated isothermal amplification and electrochemical DNA sensorM. U. Ahmed, Q. Hasan, M. M. Hossain, M. Saito, E. TamiyaFood Control, 21, 599, 2010
Highly Sensitive Method for Electrochemical Detection of Silver Nanoparticle Labels in Metalloimmunoassay with Preoxidation/Reduction Signal Enhancement, Miyuki CHIKAE, Koutarou IDEGAMI, Naoki NAGATANI, Eiichi TAMIYA and Yuzuru TAKAMURA, Electrochemistry, vol.78 No.9 SEP. 2010, P.748-753
Rapid detection for primary screening of influenza A virus: microfluidic RT-PCR chip and electrochemical DNA sensorK. Yamanaka, M. Saito, K. Kondoh, M. M. Hossain, R. Koketsu, T. Sasaki, N. Nagatani, K. Kkuta, E. TamiyaAnalyst, 136, 2064, 2011
Semi-real time electrochemical monitoring for influenza virus RNA by reverse transcription loop-mediated isothermal amplification using a USB powered portable potentiostatN. Nagatani, K. Yamanaka, M. Saito, R. Koketsu, T. Sasaki, K. Ikuta, T. Miyahara, E. TamiyaAnalyst, 136, 5143, 2011
Semi-quantitative detection of gene expression using bisbenzimide dyeP. Kittimongkolsuk, T. Tencomnao2, R. SantiyanontGenet. Mol. Res. 2011
Development of Label-Free Impedimetric Hcg-Immunosensor Using Screen-Printed ElectrodeTruong TN Lien, Nguyen Xuan Viet, Miyuki Chikae, Yoshiaki Ukita and Yuzuru TakamuraJ Biosens Bioelectron, 2:3, 2011
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Electrochemical detection of specific DNA and respiratory activity of Escherichia coliK. Yamanaka, T. Ikeuchi, M. Saito, N. Nagatani, E. TamiyaElectrochimica Acta, 82, 132, 2012
A thiophene-containing compound as a matrix for matrix-assisted laser desorption/ionization mass spectrometry and the electrical conductivity of matrix crystalsA.Yasuda,a T.Ishimaru,a S.Nishihara, M.Sakai, H.Kawasaki,R.Arakawa and Y.Shigeria,Eur. J. Mass Spectrom. 19, 2013
Gold-linked electrochemical immunoassay on single-walled carbon nanotube for highly sensitive detection of human chorionic gonadotropin hormoneNguyen Xuan Viet, Miyuki Chikae, Yoshiaki Ukita, Kenzo Maehashi, Kazuhiko Matsumoto, Eiichi Tamiya, Pham Hung, Yuzuru Takamura, Biosensors and Bioelectronics, 42, 592-597, 2013
Quantitative detection for Porphyromonas gingivalis in tooth pocket and saliva by portable electrochemical DNA sensor linked with PCR,K.Yamanaka, S.Sekine, T.Uenoyama, M.Wada, T.Ikeuchi, M.Saito, Y.Yamaguchi, E.Tamiya,Electroanalysis, 2686-2692 2014
, , 44,126-134(2015), Electrochemistry, 83(1) , 24-29 (2015), 65(10) 40-50 (2014), 51(1) 2-10 (2014)
Article list using DEP-Chips III
1Products and Service ListCompact Potentiostat BDTminiSTAT100 4Compact Multi-channel PotentiostatBDTminiSTAT400, BDTminiSTATSR-6 6 7Advantage of DEP-Chips 9Application examples of BDTminiSTAT100 and DEP-Chips 11 12An example of DNA sensor system 14 15Gold-Linked Electrochemical Immuno-Assay (GLEIA, PCT/JP2007/56992, JP5187759) 17Application ofGold-Linked Electrochemical Immuno-Assay IGlucose sensor with Glucose Dehydrogenase using DEP-Chips (Chrono-amperometry) 20 21Article list using DEP-ChipsArticle list using DEP-Chips II 24