biochemistry
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Biochemistry. Lecture 12. Energy from Reduced Fuels is Used to Synthesize ATP in Animals. Carbohydrates, lipids, and amino acids are the main reduced fuels for the cell Electrons from reduced fuels used to reduce NAD + to NADH or FAD to FADH 2 . - PowerPoint PPT PresentationTRANSCRIPT
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Biochemistry
Lecture 12
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Energy from Reduced Fuels is Used to Synthesize ATP in
Animals• Carbohydrates, lipids, and amino acids are the
main reduced fuels for the cell
• Electrons from reduced fuels used to reduce NAD+ to NADH or FAD to FADH2.
• In oxidative phosphorylation, energy from NADH and FADH2 are used to make ATP
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• Proteins that mediate e- transport & oxidative phosphorylation are integrally bound to the inner membrane– Liver mitochondria few cristae( respiration)– Heart mito. many cristae ( respiration)
• TCA cycle enzymes & metabolites are located in the matrix.
24H+ + 24e- + 6O2
O
CH2OH
OHOH
OH
OH+ 6H2O 6CO2 + 24H+ + 24e-
12H2O
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Oxidation of NADH by O2 is Highly Exergonic
NADH NAD+ + H+ + 2e- E° = +0.32½ O2 + 2e + 2H+ H2O E° = +0.82
½ O2 + NADH + H+ H2O + NAD+ E° = +1.14 V
therefore:
∆G° = -nFE° = -2 x 23 kcal/mol/V x 1.14 V = -53 kcal/mol
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II
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II
FADH2FAD
Rotenone/ amytal
Antimycin aCN
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The Oxidation of NADH or FADH2 by O2 is Tightly Coupled to the Phosphorylation of ADP
2 NADH
1 O2
nADP + nPi
nATP
How many moles of ATP are synthesized from the reduction of 1 mole O2 ?
• Measure the amount of O2 consumed (reduced to 2H2O) for any given amount of ADP added.
• Experimental Conditions
- same as the inhibitor expt (no ADP initially, excess PO4)
- isolated mito’s in buffer containing excess phosphate
- addition of ADP + an electron donor starts electron transport
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“Artificial Respiration”: Experiments that led to Understanding the sequence of Electron Transport Proteins
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O2 Consumption as a function of ADP P’n
Conditions: Isolated mitochondria in buffer containing excess PO4. Reaction is initiated by addition of ADP and e- donor.
(90 micromoles)
18 umoles
30 umoles
45 umoles
ADP=90 umoles
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Interpretation of Results
a) -OH-butyrateConversion of 90 umol ADP (or PO4) ATP requires 18 umol O2 (36 umol O)P/O = 90/36 = 2.5
b) SuccinateConversion of 90 umol ADP (or PO4) ATP requires 30 umol O2 (60 umol O)P/O = 90/60 = 1.5
c) TMPD/Ascorbate P/O = 90/90 = 1
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II
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II
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Complex II
Q
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• Oxygen is a bi-radical• Can accept e-s only 1 at a time• ETC starts with e- pairs….
• O2 •
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II
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Complex IV (cytochrome oxidase)
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II
44 2
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Evidence that supports the chemiosmotic hypothesis:
1. e- transport correlates with generation of a proton gradient
2. An artificial pH gradient leads to ATP synthesis in intact mitochondria
3. Complex I,III, and IV are proton pumps4. A closed compartment is essential5. Proton carriers (across IMM) “uncouple”
oxidation from P’n.
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ATP Synthase
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Coupling H+ translocation through ATP Synthase with ATP synthesis
Free energy of H+ translocation “forces” an internal cam shaft to rotate, which changes the conformation of each subunit during one complete turn.
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Inhibitors of Oxidative Phosphorylation1. Inhibitors of Complexes I, III, & IV.2. Oligomycin – antibiotic which binds to ATP synthase and
blocks H+ translocation.3. Uncouplers:
a) Dinitrophenol (DNP).
OHO2N
O2NIMS MATRIX
OO2N
O2N
H+
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b) Ionophores
i) Valinomycin – carries charge but not H+’s.
- Dissipates electrical gradient. ii) Nigericin – carries protons but not charge.
- Dissipates chemical gradient. (due to H+)
M+
M+ = K+ >> Na+
M+
H+
M+ = K+ >> Na+
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c) Thermogenin – active component of brown fat.- acts as a H+ channel in the IMM of brown fat mitochon. P/O << 1.
Regulation of Thermogenin Conductance
H+H+H++
FFA’S+ glycerol
TAG
HSL +
Norepinephrine
**Uncoupling (and heat generation) occur only if plenty of FFA substrate is available. If not, ATP synthesis prevails.