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BIOCHEMICAL TESTS

Rona Arianne B. Romanes

SIMSulfur ReductionIndole ProductionMotility

CITRATE UTILIZATIONto determine if an organism is capable of utilizing citrate as sole carbon source for metabolism with resulting alkalinity.Indicator: bromthymol blue

LIAused to determine whether a gram-negative rod decarboxylases or deaminases lysine and forms hydrogen sulfide. Indicator: bromcresol purple

TSIdetect microorganisms that can breakdown carbohydrates anaerobicallyIndicator: Phenol red and Ferrous sulfateIncubation: 33 - 37C for 18 to 24 hours

UREASEdetermine bacteria that has enzyme urease that can hydrolyze urea into: CO2, H2O and NH3Indicator: phenol redMedia: Stuarts or Christens media

BHI(BHIB) is a liquid medium rich in nutrients, suitable for the cultivation of several fastidious strains of bacteria, such as Streptococci, Meningococci and Pneumococci, fungi and yeasts.BHIB is recommended in Standard Methods for water testing and in antimicrobial susceptibility tests.

CATALASEcatalase mediates the breakdown of H2O2 into oxygen and water.

COAGULASEfor differentiation of S. aureus from coagulase-negative staphylococci.