biochem 3: ph & proteins
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Bellringer
List an example of an acid or base you are familiar with in everyday life.
What do you know about the pH scale?
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AgendaCollection Time
Book Project & Macromolecule notesCandy Molecules lab
Quick Experiment: Milk and Vinegar
Guided Notes: pH & the Effect on ProteinCompare Acids and BasesReview the use of pH scaleDescribe how changes in pH affect proteins
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Properties of Acids
Sour taste
Corrosive to metals
Burns the Skin
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Examples of Acids
Acetic Acid in Vinegar
Citric Acid in Oranges and Lemons
Hydrochloric Acid dissolves food in your stomach
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Properties of BasesBitter Taste
Feel “Soapy”
Will irritate your skin
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Examples of Bases
Milk of Magnesia
Bleach
Many Soaps
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Acid/Base Chemistry
Acids release Hydrogen ions [H+] when mixed with water.
Bases absorb Hydrogen ions [H+] when mixed with water.
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Neutralizations
Since Acids produce [H+] and Bases absorb [H+], it is possible for acids and bases to cancel one another.
When acids and bases cancel out it is called Neutralizing
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pH Scale
The strength of Acids and Bases is measured using the pH scale
Scale goes from 0 to 14ACIDS = below 7NEUTRAL = exactly 7BASES = above 7
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pH and Strength
The further an acid or base is from 7, the stronger it is.
ExamplesLemon Juice (2.0) is a stronger acid than
Beer (4.0) because a pH of 2 is farther from 7.
Ammonia (11.0) is a stronger base than Baking Soda (8.3) because a pH of 11 is farther from 7.
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BrainPOP: Acid/Base
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Protein Folding
Proteins are long chains of amino acids.
These chains bend and fold into a certain shape that allows them to perform their job.
The shape of the protein is held together by bonds between amino acids on different parts of the chain.
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Protein Structure Video Clip
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How Acids Denature Proteins
Acids destroy proteins by “denaturing” them. This means that the protein gets unwrapped and the shape is destroyed.
Acids can unwind proteins because the H+ [Hydrogen ions] interfere with the bonds that hold the protein together.
For this reason, it is important for living things to maintain a consistent pH level in bodily fluid. For example, human blood must stay at a pH of 7.4
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H+
H+
H+
H+
H+
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Explain what caused the milk to curdle. . .
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Assignments
12 Review Questions
Using pH to identify liquids
Students should complete their Quarterly Assessment if not finished yesterday.