bioba solution infographic #3 final linksmethods + training = increased productivity jump start your...

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Save time and money with increased productivity What Makes Large Molecule Sample Prep so Difficult? Improve Reproducibility and Efficiency: Save Time and Money Tedious manual steps for enrichment, digestion and clean-up can result in high variability ~15 manual steps High Reproducibility ~7% CVs Bioanalysis studies have large sample numbers 100’s of samples Every new biologic requires a specific sample prep strategy: sourcing reagents and researching protocols Up to 2 months to develop 10 20 30 40 50 60 No. of Injections Peak Area Ratio 15 10 5 0 AB Sciex is doing business as SCIEX. © 2016 AB Sciex. For research use only. Not for use in diagnostic procedures. The trademarks mentioned herein are the property of the AB Sciex Pte. Ltd. or their respective owners. AB SCIEX™ is being used under license. Beckman Coulter product and service marks mentioned herein are trademarks or registered trademarks of Beckman Coulter, Inc. in the United States and other countries. SCIEX and Beckman-Coulter are Danaher Life Science companies. RUO-MKT-07-3771-A | AAG-1580FLY03.16-A Make the Move to Simplified Sample Prep Biologics Bioanalysis: Reagents and Buffers Beckman Coulter Biomek Automation Pre-built methods that map to your compound of interest Icon-based, guided set-up Monitor remotely Faster Method Development Digestion Enrichment Overcome Sample Prep Challenges with the BioBA Solution and Biomek Automation 1 click automation All-in-One kit for 100 samples BioBa Sample Prep Kit Protocols BioBA Solution C O M P L E X B I O L O G I C S I N T E G R A T I O N LC-MS Systems and Software New Methods Sample Preparation and Automation Learn more at sciex.com/biobasolution

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Page 1: BioBA Solution Infographic #3 Final LINKSMethods + Training = Increased Productivity Jump Start Your Workflows Biologics Bioanalysis: BioBA Solution C O M P L E X G B I O L O I C

Save time and money with increased productivity

What Makes Large Molecule Sample Prep so Difficult?

Improve Reproducibility and Efficiency: Save Time and Money

Tedious manual steps for enrichment, digestion and clean-up can result in high variability

~15 manual steps

High Reproducibility~7% CVs

Bioanalysis studies havelarge sample numbers

100’s of samples

Every new biologic requires a specific sample prep strategy: sourcing reagents and researching protocols

Up to 2 months to develop

10 20 30 40 50 60

No. of Injections

Peak

Are

a R

atio

15

10

5

0

AB Sciex is doing business as SCIEX. © 2016 AB Sciex. For research use only. Not for use in diagnostic procedures. The trademarks mentioned herein are the property of the AB Sciex Pte. Ltd. or their respective owners. AB SCIEX™ is being used under license.

Beckman Coulter product and service marks mentioned herein are trademarks or registered trademarks of Beckman Coulter, Inc. in the United States and other countries.

SCIEX and Beckman-Coulter are Danaher Life Science companies.

RUO-MKT-07-3771-A | AAG-1580FLY03.16-A

Make the Move to Simplified Sample PrepBiologics Bioanalysis:

Reagents andBuffers

Beckman Coulter Biomek Automation

• Pre-built methods that map to your compound of interest

• Icon-based, guided set-up• Monitor remotely

Faster Method Development

DigestionEnrichment

Overcome Sample Prep Challenges with the BioBA Solution and Biomek Automation

1 click automation

All-in-One kit for 100 samples

BioBa Sample Prep Kit

Protocols

BioBASolution

CO

MP

LE

X

BI O

L O G I C S

I N T E G

RA

TI

ON

LC-MSSystems

andSoftware

NewMethods

SamplePreparation

and Automation

Learn more at sciex.com/biobasolution

Page 2: BioBA Solution Infographic #3 Final LINKSMethods + Training = Increased Productivity Jump Start Your Workflows Biologics Bioanalysis: BioBA Solution C O M P L E X G B I O L O I C

Large Molecules Present Unique LC-MS Challenges

AB Sciex is doing business as SCIEX. © 2016 AB Sciex. For research use only. Not for use in diagnostic procedures. The trademarks mentioned herein are the property of the AB Sciex Pte. Ltd. or their respective owners. AB SCIEX™ is being used under license.

RUO-MKT-07-3848-A

Quantitation You Can Count OnBiologics Bioanalysis:

+3

+4

+2

The BioBA Solution LC-MS Systems and Software Drive Productivity

Automated Peptide MRM Method Optimization with DiscoveryQuantTM Software

Powerful Peptide Quantitation with MultiQuant™ Software

Significantly Reduce method development time

High Throughput infusion or on-column optimization

Signal

High quality peak picking for fast, accurate quantitation

QTRAP® 6500+ MS System with ExionLC™ System handles biologic quant challenges.

Ensure data security and compliance » Designed to support 21 CFR

part 11 compliance » LIMS compatible

Inte

nsity

Time

For high sensitivity applications, use the M3 microLC system.

Best-in-Class Sensitivity

Enhanced Selectivity with SelexION® DMS or MRM3 technology

5-6 Orders of Linear Dynamic Range

Data Analysis

Method Development

» Chromatography » Multiple Charge States » Declustering Potential » Collision Energy

Need Efficient MRM Optimization Low abundance

of target in samples

Need Maximal

Sensitivity

High interference and background from other proteins

Need Enhanced Selectivity

Large sample numbers and batches

Need Simple Software for

Complex Data

10 pg/mLglucagon

S/N-22.1

MRM

MRM withSelexION

BioBASolution

CO

MP

LE

X

BI O

L O G I C S

I N T E G

RA

TI

ON

SamplePreparation

and Automation

NewMethods

LC-MSSystems

and Software

Page 3: BioBA Solution Infographic #3 Final LINKSMethods + Training = Increased Productivity Jump Start Your Workflows Biologics Bioanalysis: BioBA Solution C O M P L E X G B I O L O I C

Why Does it Take so Long to Implement Assays?

SCIEX iMethodTM Applications and Experts Get You Up and Running Fast

Methods + Training = Increased Productivity

Jump Start Your WorkflowsBiologics Bioanalysis:

BioBASolution

CO

MP

LE

X

BI O

L O G I C S

I N T E G

RA

TI

ON

LC-MSSystems

andSoftware

SamplePreparation

and Automation

NewMethods

and Training

Develop SamplePrep Protocol

Tested LC-MSSettings

Data Processing Procedures

Source Reagents

Time and Money spent

60

0 4.8

4.92

Time, min

5.0 5.2

Optimize LC-MS Methods

Train Scientists

+ + + =

AB Sciex is doing business as SCIEX. © 2016 AB Sciex. For research use only. Not for use in diagnostic procedures. The trademarks mentioned herein are the property of the AB Sciex Pte. Ltd. or their respective owners. AB SCIEX™ is being used under license.

RUO-MKT-07-3975-A

Ready-to-use, lab-proven iMethod Applications

» Adalimumab » Infliximab » Trastuzumab » Insulin glargine » Glucagon

Pre-built Methods Include:

2. BioBA Sample Prep Kit

4. LC-MS Setup, Running and Data Processing

5

5. Training Certification

3. Sample Prep StrategiesHands-on Lab Sessions

3

1. Large Molecule BasicsLecture

1

4

Reagents and Vendors Defined

160

0 4.8

4.92

Time, min

5.0 5.2

3

Sample Prep Steps in Detail

2 BioBa Sample Prep Kit

SCIEX Expert-Run BioBA Certification ProgramOn-site 3 day training course Includes:

+ + +=

Ensure Consistent Results

Portability Across Labs & Groups

Maximize Productivity

2 Month Project Development Time vs.2 Week iMethod Application Implementation

VS

Save Time and Money

00:00

2 4