bioba solution infographic #3 final linksmethods + training = increased productivity jump start your...
TRANSCRIPT
Save time and money with increased productivity
What Makes Large Molecule Sample Prep so Difficult?
Improve Reproducibility and Efficiency: Save Time and Money
Tedious manual steps for enrichment, digestion and clean-up can result in high variability
~15 manual steps
High Reproducibility~7% CVs
Bioanalysis studies havelarge sample numbers
100’s of samples
Every new biologic requires a specific sample prep strategy: sourcing reagents and researching protocols
Up to 2 months to develop
10 20 30 40 50 60
No. of Injections
Peak
Are
a R
atio
15
10
5
0
AB Sciex is doing business as SCIEX. © 2016 AB Sciex. For research use only. Not for use in diagnostic procedures. The trademarks mentioned herein are the property of the AB Sciex Pte. Ltd. or their respective owners. AB SCIEX™ is being used under license.
Beckman Coulter product and service marks mentioned herein are trademarks or registered trademarks of Beckman Coulter, Inc. in the United States and other countries.
SCIEX and Beckman-Coulter are Danaher Life Science companies.
RUO-MKT-07-3771-A | AAG-1580FLY03.16-A
Make the Move to Simplified Sample PrepBiologics Bioanalysis:
Reagents andBuffers
Beckman Coulter Biomek Automation
• Pre-built methods that map to your compound of interest
• Icon-based, guided set-up• Monitor remotely
Faster Method Development
DigestionEnrichment
Overcome Sample Prep Challenges with the BioBA Solution and Biomek Automation
1 click automation
All-in-One kit for 100 samples
BioBa Sample Prep Kit
Protocols
BioBASolution
CO
MP
LE
X
BI O
L O G I C S
I N T E G
RA
TI
ON
LC-MSSystems
andSoftware
NewMethods
SamplePreparation
and Automation
Learn more at sciex.com/biobasolution
Large Molecules Present Unique LC-MS Challenges
AB Sciex is doing business as SCIEX. © 2016 AB Sciex. For research use only. Not for use in diagnostic procedures. The trademarks mentioned herein are the property of the AB Sciex Pte. Ltd. or their respective owners. AB SCIEX™ is being used under license.
RUO-MKT-07-3848-A
Quantitation You Can Count OnBiologics Bioanalysis:
+3
+4
+2
The BioBA Solution LC-MS Systems and Software Drive Productivity
Automated Peptide MRM Method Optimization with DiscoveryQuantTM Software
Powerful Peptide Quantitation with MultiQuant™ Software
Significantly Reduce method development time
High Throughput infusion or on-column optimization
Signal
High quality peak picking for fast, accurate quantitation
QTRAP® 6500+ MS System with ExionLC™ System handles biologic quant challenges.
Ensure data security and compliance » Designed to support 21 CFR
part 11 compliance » LIMS compatible
Inte
nsity
Time
For high sensitivity applications, use the M3 microLC system.
Best-in-Class Sensitivity
Enhanced Selectivity with SelexION® DMS or MRM3 technology
5-6 Orders of Linear Dynamic Range
Data Analysis
Method Development
» Chromatography » Multiple Charge States » Declustering Potential » Collision Energy
Need Efficient MRM Optimization Low abundance
of target in samples
Need Maximal
Sensitivity
High interference and background from other proteins
Need Enhanced Selectivity
Large sample numbers and batches
Need Simple Software for
Complex Data
10 pg/mLglucagon
S/N-22.1
MRM
MRM withSelexION
BioBASolution
CO
MP
LE
X
BI O
L O G I C S
I N T E G
RA
TI
ON
SamplePreparation
and Automation
NewMethods
LC-MSSystems
and Software
Why Does it Take so Long to Implement Assays?
SCIEX iMethodTM Applications and Experts Get You Up and Running Fast
Methods + Training = Increased Productivity
Jump Start Your WorkflowsBiologics Bioanalysis:
BioBASolution
CO
MP
LE
X
BI O
L O G I C S
I N T E G
RA
TI
ON
LC-MSSystems
andSoftware
SamplePreparation
and Automation
NewMethods
and Training
Develop SamplePrep Protocol
Tested LC-MSSettings
Data Processing Procedures
Source Reagents
Time and Money spent
60
0 4.8
4.92
Time, min
5.0 5.2
Optimize LC-MS Methods
Train Scientists
+ + + =
AB Sciex is doing business as SCIEX. © 2016 AB Sciex. For research use only. Not for use in diagnostic procedures. The trademarks mentioned herein are the property of the AB Sciex Pte. Ltd. or their respective owners. AB SCIEX™ is being used under license.
RUO-MKT-07-3975-A
Ready-to-use, lab-proven iMethod Applications
» Adalimumab » Infliximab » Trastuzumab » Insulin glargine » Glucagon
Pre-built Methods Include:
2. BioBA Sample Prep Kit
4. LC-MS Setup, Running and Data Processing
5
5. Training Certification
3. Sample Prep StrategiesHands-on Lab Sessions
3
1. Large Molecule BasicsLecture
1
4
Reagents and Vendors Defined
160
0 4.8
4.92
Time, min
5.0 5.2
3
Sample Prep Steps in Detail
2 BioBa Sample Prep Kit
SCIEX Expert-Run BioBA Certification ProgramOn-site 3 day training course Includes:
+ + +=
Ensure Consistent Results
Portability Across Labs & Groups
Maximize Productivity
2 Month Project Development Time vs.2 Week iMethod Application Implementation
VS
Save Time and Money
00:00
2 4