bioaugmentation 101: purpose, concepts and mechanisms · fats oils grease • 40-60% of wwtp...

BIOAUGMENTATION 101: Purpose, Concepts and

Mechanisms

Ask Yourself:

Working for, or around, the

biology of a systemcan generate high costs and reduce

efficiency.

Am I working for my biology?

OR

Is my biology

working for me?

Is Biology Performance Costing $$$?

• 40-60% of WWTP operating costs related to sludge management

• Vacuum trucks to clean out lift stations, greasetraps, sewers, etc.

SOLIDS

• Fines for H2S • $2K/ 10 min in

state of MN

• Tourism losses at vineyards and resorts

ODORS

• Capital equipment malfunctions and inefficiencies

• Collection and disposal costs

FATS, OILS, GREASE

Scientifically Selected

Bio:

Augment:

Relating to life

Make greater by adding to it

Definition:

“The process of adding scientifically selected organisms into a microbial community in order to enhance that community.”

What if we could add to our biology to improve it’s function?

Bioaugmentation

Scientifically Selected

Selection Tools

Strain Enzyme Screening

Library Strain Comparisons

1500 Substrate Assays Genome Data

Microbial Community

GrowthSubstrate Digestion

Unwanted By-ProductsProblem Causing Organisms

Incr

ease

Decrease

“Enhance That Community”

Organic Solids

Odor

FOG

• System capacity limitations

• Toxic upset recovery• Difficult to degrade

material • Foam• Nutrient removal

What Issues Does Bioaugmentation Solve?

• Promotes surrounding population’s growth (more workers = more work)• Survival across broad range of environments & substrates (spore former)

Population Growth and Stability

• General: Amylase, protease, xylanase, cellulase, glucosidase, lipase, esterase, urease• Specialty: Ligninase, laccase, arabinoxylanase, mannosidase, phytase,

monooxygenases, galactosidase, and pectatelyase

Enzymes Production

• Ammonia assimilation, sulfur utilization, phosphate assimilation, aromatic hydrocarbon utilization

• Genes involved with arsenic & cyanide

Biochemical Processes

• Aerobic, anoxic and anaerobic growthMetabolism Methods

Broad Mechanisms

WHAT MAKES YOUR BUGS SO SPECIAL?

Let’s Focus on the BIG 3!

• Capital equipment malfunctions and inefficiencies

• Collection and disposal costs

SOLIDS ODORSFATS OILS

GREASE

• 40-60% of WWTP operating costs related to sludge management

• Vacuum trucks to clean out lift stations, grease traps, sewers, etc.

• Fines for H2S • $2K/ 10 min in

state of MN!!

• Tourism losses at winery and vineyards during “crush season”

Sludge/ bio-solids

Living Cells Non-living mass

• Dead cell material

• Biopolymers

• Bacteria • Protozoa • Metazoan• Fungi &

yeast

Biomass Insoluble particulate & FOG

Organic Inorganic

Solids - Components of sludge

Potential Food Source

Real Science. Trusted Process. Proven Success.11

Bioaugmentation and sludge

How does bioaugmentation remove sludge?

Bacteria

Micronutrients

+

The Product

Produces enzymes and attacks sludge as a source of CNP

Sludge

Enzymes produced by Bacillus are like the tools (knife and fork) that convert longer chain compounds into more bite size smaller chain substrates.

Cofactors for digestionThese can be a “missing link” in metabolic processes

Sludge is exists in varying aerobic, anoxic and anaerobic conditions so it is good to have a bacteria that can do all three

+ (if available)

Facultative Anaerobe

Real Science. Trusted Process. Proven Success.

• WAS is typically 0.5-1.5% solids (~99% water)• Potential for water removal is related to floc size

and consistency

Solids - sludge dewatering

Belt press can produce 9-25% solids

Poor Dewatering Good Dewatering


Picture reducing FOG cap. How FOGlayer develops on top and how product can reduce problem.

Layer develops after natural flora digests only protein.

Rendering Waste FOG

Treated

Fats, Oils and Grease (FOG’s)

6 Bacillus strains in Ultra product

• V81: 75 genes for lipid and fatty acid metabolism

• 408: 77 genes• Lipases, esterases, and enzymes

involved in long chain fatty acid digestion

• Biosurfactant production• Helps overcome

hydrophobic/hydrophilic interface


Mode Of Action

HYDROCARBONUSE

MICRONUTRIENTS BIOSURFACTANTS

• C:N:P liberation • Enzymes: Oxygenase

• Essential cofactors to hydrocarbon digestion

• Same strains that produce biosurfactants for FOG digestion, also degraded petroleum hydrocarbons

• Makes sense FOG is also a hydrocarbon


• H2S is formed by sulfate reducing bacteria (SRBs)– Use SO4 as an electron acceptor instead of O2

– Live in biofilms and feed on FOGs and VFAs • Dominate in uncontrolled anaerobic conditions

• Other odors you smell are actually volatile fatty acids (VFAs)– Acid forming bacteria sometimes require anaerobic environments and

tend to live in biofilms

15

Odor: VFA and H2S

Real Science. Trusted Process. Proven Success.16

Biofilm Cross-Section


• Bacillus strains in our products have >20 year history of reducing VFA and H2S in animal lagoons

• Now that we can sequence the genomes we see the many genes involved with VFA and sulfur utilization– The sulfur utilization wasn’t surprising, the fact that sulfur

utilization genes outnumber many others genes was

17

Odor: VFA and H2S


• Sulfur utilization– Less sulfur available for conversion to H2S

• Removal of biofilms and sludge housing SRBs and AFBs

• VFA and FOG utilization pathways – Limits VFA addition to odors as well as food source to SRBs

18

Modes of Action


Modes of Action Cont’

19

H2S

SO4

VFA

VFA

VFAS

Real Science. Trusted Process. Proven Success.5200 W. Ashland Way, Franklin, WI mdgbio.com1-888-WE-FERMENT


Thank YouQuestions?

bioaugmentation 101: purpose, concepts and mechanisms · fats oils grease • 40-60% of wwtp...

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