Assay Design Inputs 1

BioAssay Works, LLC10075 Tyler Place, Suite 18Ijamsville, MD 21754 USA

BioAssay Works, LLC requests your help. The information provided below helps us better understand your requirements for a rapid assay and serves as a starting point for our development proposal and key deliverables for any subsequent development project.

Customer Name: Project Name: Proj. Manager/Tel:, email: Date:[Customer Name]

[Project Name]

[Date]

1. The target(s) you wish to detect is/are:

Antibodies Antigens/biomarker

Comments:

a) Target Information

Name Size (kDa)Target 1Target 2Target 3

b) Antibodies/Analytes required for detection:

I have Antibodies/Analytes for detection

BioAssay Works to source

If you have antibodies/analytes, please share more information about your antibodies/analytes in the table below:

Name Host Species Poly/mAb Ig Class Qty.* (mg/ug)

Comments: *A concentration of >2 mg/mL is required for best results.

Assay Development Design

Inputs

Assay Design Inputs 2

2. Assay format:

a) Please select your preferences in the table below:

Cassette-based Dipstick No preference Qualitative (Y/N) Semi-quantitative

(reader) No preference

Sandwich Assay Competitive Assay No preferenceAdditional information:

b) Where will the assay be performed (laboratory, point-of-use, etc.)

Please describe:

3. Sample to be tested:

a) Sample matrix (serum, blood, urine, etc.)?

Please describe:

b) Sample handling, treatment, or collection expectations?

Please describe:

c) Volume of sample typically available for testing? ______________L

4. Assay Performance:

a) Please indicate your requirements for assay performance below. If you have no requirements at this point, please leave blank.

Parameter Optimal Requirement

Minimal Requirement

Rank1 =

Desirable2 =

Important3 = Crucial

Sensitivity/Limit of detectionSpecificity/cross-reactivityTime to read results (minutes)Stability

Assay Design Inputs 3

5. Other Requirements/Comments:

[Add your comments here.]