1) Briefly describe the main mechanism by which carbohydrates are used as nutrient for microbial growth

2) Lineweaver - Burke plot OR (Double reciprocal plot)

5) Nitrogen Fixation Metabolism a) Write the equation for nitrogen fixation. N2 + 6 H+ + 6 e 2 NH3b) Write the name of one microorganism that works in symbiosis with plants to provide them nitrogen. Azotobacter [Facultative Bacillus Anaerobe Clostridia]c) Does this microorganism require oxygen to fix nitrogen? Yes d) Enzyme used in nitrogen fixation reaction Nitrogenase