bio pacrim conference ogden.pdfvitamin solution (100 x) vitamin solution 0.005 0.005 ml common name...
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BIO PacRim conferenceContact: Kimberly Ogden
Department of Chemical and Environmental EngineeringUniversity of ArizonaTucson, Arizona USA
Project Goals
• Obtain long term algal cultivation data in outdoor pond systems
• Work with industrial, government, and academic partners to advance the algal biofuels, feed, and bio-products industry
• Optimize biomass and lipid content for production of biofuel using impaired waters
• Develop real time sensors and control strategies for efficient cultivation
• Improve and refine cultivation models, as well as system techno-economic models and life cycle assessments.
Testbed Facilities
ARID Raceway US Patent # 8,245,440
Ability to Cultivate GMO Algae in Climate Controlled Green
Houses
Testbed Facilities
Hot house with raceway for seasonal extension
Raceways and Solix System - Centrifuge harvester
Testbed Facilities
Multi-scale Testbed Facility with Harvesting Infrastructure
Cultivation of Chlorella sorokinana
2014
Media Optimization
Required volume (L) = 1
Chemical Common name g/L Total Grams Total Kilograms
Na2CO3 Soda Ash 0.02 0.02 0.00002
NaCl TruSoft 5 5 0.005
(NH2)2CO Urea 0.05 0.05 0.00005
MgSO4 * 7H2O Magnesium sulphate 0.012 0.012 0.000012
NH4H2PO4 MAP 0.01 0.01 0.00001
KCl Potash 0.129 0.129 0.000129
FeCl Iron Chloride 0.0035 0.0035
Di Sodium EDTA 0.00436 0.00436
Trace metals solution 1 1 0.001
Vitamin solution (100 X) Vitamin solution 0.005 0.005 ml
Common name g/L Vendor
Boric Acid 0.00286 VWR
Manganese Chloride 0.00181 VWR
Zinc Sulfate 0.000222 VWR
Copper Sulfate 0.000079 VWR
Cobalt Nitrate 0.0000494 VWR
Sodium Molybdate 0.000391 VWR
Common name g/L Vendor
Vitamin B12 0.135 VWR
Manganese Chloride 0.025 VWR
Zinc Sulfate 0.335 VWR
Trace metals solution
Vitamin solution
Pe-06 (or Pe-02_B)
Semi continuous Operation
0
0.5
1
1.5
2
2.5
3
3.51
0/1
5/1
4
10
/17
/14
10
/19
/14
10
/21
/14
10
/23
/14
10
/25
/14
10
/27
/14
10
/29
/14
10
/31
/14
11
/2/1
4
11
/4/1
4
11
/6/1
4
11
/8/1
4
11
/10
/14
11
/12
/14
11
/14
/14
11
/16
/14
11
/18
/14
11
/20
/14
11
/22
/14
11
/24
/14
OD
75
0
Continuous pH, Temp, DO
-10
-5
0
5
10
15
20
25
30
35
40
45
0
1
2
3
4
5
6
7
8
9
10
10/18/2014 10/23/2014 10/28/2014 11/2/2014 11/7/2014 11/12/2014 11/17/2014 11/22/2014 11/27/2014
pH
OD 750
DO
Temp C
CO2 input
0.00
1.00
2.00
3.00
4.00
5.00
6.00
7.00
8.00
9.00
10.00
10/24/2014 10/26/2014 10/28/2014 10/30/2014 11/1/2014 11/3/2014 11/5/2014
Cu
mm
ula
tive
CO
2 (
L)
pH
an
d O
D 7
50
OD 750
pH
CO2
Culture Diagnostics
• PCR diagnostics of invasive strains identified by 18S rRNA gene fragment sequencing
• Specific primers for algae and bacterial contaminants
• Flow Cam Systems for monitoring
• Real time OD monitor and flow cell –Invention disclosure and patent
• Contaminant Control to maintain Chlorella sp.
Molecular Based Culture Diagnostics
Testbed Facilities
Climate Controlled Cultivation Systems for Growth and Biomass
Optimization and Modeling
Characterizing Cold Weather Strains
Opportunities for Collaboration
• Production of algal biomass for a variety of applications – Fuel– Feed– High value Products
• Cultivation of genetically modified algae
• Design and implementation of integrated, controlled systems for cultivation, harvesting, and conversion.
• Optimization of algal productivity in impaired waters
• Culture diagnostics using molecular markers