bio 230 - microbiology - spring 2013 study guide 13simon/bio230/class/mic_class13_13.pdfbio 230 -...
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Bio 230 - Microbiology - Spring 2013
Study Guide 13
http://www.answersingenesis.org/docs2004/images/xylitol_comic.gif
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How DNA Sequencing Works
http://seqcore.brcf.med.umich.edu/doc/educ/dnapr/sequencing.html
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http://seqcore.brcf.med.umich.edu/doc/educ/dnapr/sequencing.html
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http://seqcore.brcf.med.umich.edu/doc/educ/dnapr/sequencing.html
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Part of a radioactively labelled sequencing gel
http://en.wikipedia.org/wiki/DNA_sequencing
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Reaction with all four of the dideoxy nucleotides (A, G, C and T)
present, and with different fluorescent colors on each.
http://seqcore.brcf.med.umich.edu/doc/educ/dnapr/sequencing.html
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One reaction can get the sequence of a fragment of DNA as long
as 700 or so nucleotides
http://seqcore.brcf.med.umich.edu/doc/educ/dnapr/sequencing.html
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DNA sequence (455 nucleotides of the lysU gene of E. coli) which
was generated by an automated sequencing device.
http://users.rcn.com/jkimball.ma.ultranet/BiologyPages/D/DNA_sequence.gif
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Overview of the 454 sequencing technology
The development and impact of 454
sequencing Jonathan M Rothberg & John H Leamon
Nature Biotechnology 26, 1117 - 1124 (2008)
Published online: 9 October 2008
doi:10.1038/nbt1485
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454 Sequencing
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454 Sequencing
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Shotgun Sequencing: Assembly of Random Sequence Fragments
http://seqcore.brcf.med.umich.edu/doc/educ/dnapr/sequencing.html
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http://cancergenome.nih.gov/media/whitehead_center_image.jpg
Large-scale Sequencing Center
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http://wishart.biology.ualberta.ca/basys/cgi/submit.pl
Automated, in-depth annotation of bacterial genomic
(chromosomal and plasmid) sequences
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Expanded view of the E. coli K-12 genome showing gene location
and COG functional categories
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Genome Resources for Bio 230
http://www.geneseo.edu/~simon/bio230/genome_resources.html
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Two-colour microarrays, the probes are
oligonucleotides, cDNA or small
fragments of PCR products
corresponding to mRNAs. This type of
array is typically hybridized with cDNA
from two samples to be compared (e.g.
patient and control) that are labeled
with two different fluorophores (e.g.
Rhodamine (red) and Fluorescein
(green)).
http://en.wikipedia.org/wiki/DNA_microarray
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http://en.wikipedia.org/wiki/DNA_microarray
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PCR
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RNA Transcription Levels Can be Validated with qPCR
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Figure 5. Distribution of cell cycle-regulated genes within functional
categories. The number of genes in each category is in parentheses.
Genes were assigned to a functional category based on the classification
scheme defined by Clusters of Orthologous Genes (COGs)
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The End