Anatomy & Physiology

BIO 205Microbiology with LabDenver School of Nursing General Education ClassesLecture / Laboratory :Monday 10:00 am 2:24pmLecture:Tuesday 4:30pm 6:30pmInstructor: Lisa Johansen, PhD Microbiology

When you see the word microbiologyWhat do you think of??MicrobiologyWhat is your connection to microbiology??Work?Home? Health?MicrobiologyChapter 1The Science of MicrobiologyThe Scope of MicrobiologySix subgroupsBacteriaArchaeaAlgaeFungi ProtozoaVirusesHelminths **Brief History of Microbiology1674Leeuwenhoek: sees microorganisms1796Jenner: vaccine for smallpox1847Semmelweiss: cause of childbed fever1859Pasteur: disproves spontaneous gen.1865Lister: introduces antiseptic technique1876Koch: pure culture on agar1892Iwanowski: discovers viruses1894Ehrlich: selective toxicity1929Fleming: discovers penicillin1977Woese: classifies archaeaAnimalculesMade his own microscopesAntony van LeeuwenhoekLooked at everything he couldWhite matter from his teeth

Edward Jenner and ImmunityObservation:Dairymaids who had mild cowpox infections were protected from smallpoxHypothesisCowpox infection provides protection against smallpoxExperimentInoculated boy with cowpox fluid and later challenged with smallpox fluidResultBoy did not get smallpox

Childbed FeverWash your hands!Ignaz SemmelweisMedical students were bring disease from the morgue to the womens clinic

Spontaneous GenerationLife is formed from inanimate objectsFruit flies!!!

Spontaneous GenerationLouis PasteurUsed swan-neck flaskBoiled brothOpen to the airNo growth unless broth was washed into the curved neck

Aseptic techniqueAgainst infection via phenolJoseph ListerHow good is the mouthwash though?

Germ Theory of DiseaseKochs PostulatesMicrobes present in samples of diseased animalGrow organism in pure cultureInject healthy animal with cultured cellsAnimal develops same disease

Viruses are discoveredSmaller than bacteria - filterableDmitri Iwanowski and Martinus Beijerinick Tobacco mosaic virus

Selective ToxicityChemotherapy Paul EhrlichMagic Bullet Theory

Penicillin The birth of antibioticsAlexander FlemingBad lab techniques made him famous

ArchaeaNot just bacteria anymoreCarl WoeseExtremophiles

Microbiology TodayDiagnosticsTreatmentsGenomicsEpidemiology Emerging diseasesBioremediationEnvironment micro / microbial ecologyGreen fuelsBioterrorismBioengineering Agricultural microbiologyIndustrial microbiology

Chapter 10meet the microbes!

Six subgroupsBacteriaArchaeaAlgaeFungi ProtozoaVirusesHelminths **

Classification systems and namesKingdom

Writing names properlybinomial nomenclaturegenus species

Escherichia coli or Escherichia coli

E. coli or E. colibacteria = binomial nomenclature plusgenus species strains

E. coli K12E. coli ML30E. coli 0157:H7

How we classify - methods - old

How we classify - Dichotomous key - an oldie but goodie

How we classify - methods - newmolecular biology / genetics

Molecular biology and identification

The Prokaryotes - Ch. 11Archaea BacteriaProkaryotes: HomeworkChose 5 bacteria (total) from different 5 different phyla (Ch. 11) and describe:

habitat - where is it normally found? shape (morphology - what does it look like under the microscope) pathogenesis (does it cause disease? if so how?) three interesting facts (not covered above)think medical or environmental importanceunique features include a picture of the organism

This must be a PowerPoint presentation.This is part of your weekly presentation grade.Due 1/14/13 @ beginning of class - on a thumb drive or email to me.

Eukaryotes and Helminths and Arthropod vectorsChapter 12

A few eukaryotes to know about:Fungimacroscopicmicroscopic

Eukaryotic cells - Fungiheterotrophic

saprobe

Eukaryotic cells - Fungiyeast colonies

mycelium

spores

A few eukaryotes to know about:Fungi

Fungal diseases : thermal dimorphoism

Mycoses = fungal infectionsringworm / tineaathletes foot / tinea

thrush

Cryptococcus

AspergillusGood Fungi

Good Fungi - antibiotic producers

A few eukaryotes to know about:Algae

A few eukaryotes to know about:Lichens

A few eukaryotes to know about:Protozoa

ParameciumAmoeba

GiardiaA few eukaryotes to know about:Protozoa: trypanosome

A few eukaryotes to know about:Protozoa: Toxoplasma gondii

A few eukaryotes to know about:Protozoa: Plasmodium

A few eukaryotes to know about:Slime molds

http://www.youtube.com/watch?v=bkVhLJLG7ug A few eukaryotes to know about:Helminths

Intestinal HelminthsEukaryotes - Helminths

Flukes

Tapeworms

Roundworms

YOU and Helminths diseases

Parasitic Helminths

A few eukaryotes to know about:Arthropod vectors

YOU and Arthropod vectors / diseasesChapter 13 - Viruses !!!!!!!!!!!

Viruses- naked vs. enveloped

Viruses - such cool shapes

Virus life cycleViruses - entry

Viruses - exit

Viruses - types of genomes

Virus classificationhttp://www.virology.ws/2009/08/07/how-viruses-are-classified/Viruses - reproduction

Viruses - reproduction

YOU and viral diseasesPoliovirus

YOU and viral diseasesInfluenza

Influenza why you have to get a immunization each year

YOU and viral diseasesHIV Human Immunodeficiency Virus

Retroviruses - reverse transcription

Retroviruses - genome integration

YOU and viral diseasesMononucleosis Epstein Barr Virus

YOU and viral diseasesHerpes Virus

YOU and viral diseases: Cancer

http://cancer.about.com/od/cancercauses/a/Viruses-And-Cancer.htmPhage / bacteriophage lytic vs. lysogenic cycle

http://biology.about.com/od/virology/ss/Bacteriophage.htmPhage / bacteriophage lytic vs. lysogenic cycle

Viruses and plants

http://www.microbiologybytes.com/virology/Plant.htmlhttp://www.sciencedaily.com/releases/2011/08/110810093833.htmhttp://en.wikipedia.org/wiki/Plant_virus http://www.ipm.iastate.edu/ipm/hortnews/2007/4-11/sick.html

What if you are already sick: Antivirals

For next week:

Your bacterial presentations - chapter 11

Multiple choice quiz chapters1 - History10 - classification12 - eukaryotes1 3 - viruses

Read chapters 2 - 3 - 4

Read Lab exercises 5, 6, 7