bibc 100 handout 9 answers
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8/6/2019 BIBC 100 Handout 9 Answers
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8/6/2019 BIBC 100 Handout 9 Answers
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BIBC 100 Structural Biochemistry TA: Lydiesther MartinezDr. Montal [email protected] 11 Section: Mon 7pm
HUMAN GROWTHHORMONE (HGH)
y GHMotif: 4-helix bundle UUDDy Induces dimerization of its specific receptor (GHR) essential for
intracellular activationy Defects in signaling pathway can lead to disease. 2 extremes
o Increased growth gigantism Acromegaly big hands, feet, jaws
o Decreased growth drawrfismy Receptor looks like immunoglobulin,
o loops connecting domains are criticaly Hormone lodged between monomers at level ofloops
o Create cavity for hormoney Receptor exists as monomerin membraney Hormone binds to ONE monomer, triggers change to surface, to
dimerizey 31 side chains are forming interaction
PROTEIN STABILITY
o Name four things helpful in providing protein stability1. Disulfied bonds/bridges2. Dipole stability3. Filing cavity of HB core4. AA change ie gly proline
MUTAGENESIS
o Engineers experimented with several strategies to stabilize the T4 Lysozyme.Which strategy worked?Interpret their results from this graph.
o Mutant T4 lysozymes were engineeredo They had either 1, 2 or 3 possible disulfide bridgeso Tm measured for oxidized and reduced states of each mutanto Results: all mutants were more stable than the wild type in
oxidized form/with disulfide bridges.o The results were additive, so that the stability increased with
the number of disulfide bridge