bc 589 - olympus

12
M514 SET UP MANUAL ST07_01.DOC 3. Check the horizontal position of the mixing bar. 1) Check the mixing bar alignment in the cuvette. (bellow fig. A). 2) Check the mixing bar alignment in the wash pot. (bellow fig. B). * Check each position and if necessary, adjust according to the Adjustment manual. A : Cuvette position B : Wash pot position 7-5 Operation check 0200

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Beckam Coutlier - Manu manual - BC

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Page 1: BC 589 - Olympus

M514 SET UP MANUAL

ST07_01.DOC

3. Check the horizontal position of the mixing bar.

1) Check the mixing bar alignment in the cuvette. (bellow fig. A).

2) Check the mixing bar alignment in the wash pot. (bellow fig. B).

* Check each position and if necessary, adjust according to the Adjustment manual.

A : Cuvette position

B : Wash pot position

7-5Operation check 0200

Page 2: BC 589 - Olympus

M514 SET UP MANUAL

ST07_01.DOC

4. Check the dead volume for the sample probe and reagent probe.

l Dead volume at rack position

Type Dead volume

Hitachi cup 50µL or less

ACA cup 250µL or less

φ13 blood collection tube 200µL or less

φ15 blood collection tube 250µL or less

l Dead volume at the DC unit position

Type Dead volume

Cup for small quantity 30µL or less

Hitachi cup 50µL or less

Hitachi cup 50µL or less

Conical cup for STAT 50µL or less

ACA cup 250µL or less

φ13 blood collection tube 200µL or less

φ15 blood collection tube 250µL or less

l Dead volume at the reagent bottle

Type Dead volume

15 mL bottle 350 µL or less

30 mL bottle 1mL or less

60 mL bottle 1mL or less

* Check each position and if necessary, adjust according to the Adjustment manual.

Operation check7-6

0200

Page 3: BC 589 - Olympus

M514 SET UP MANUAL

ST07_01.DOC

n Air extraction

• After making all the position checks, as described on the previous page, recheck the probe and mixing bar

attachment. Carefully check for looseness in the connections of the sample and reagent probe connections

• Check the syringe connection (loose the syringe case or tubes).

1) The sample and reagent dispenser is moved up/down and the air is extracted using the ANL maintenance menu.

* If the air isn’t extracted, detach the syringe case in the dispenser and extract the air by swaying the syringe by

hand.

Air extraction position Menu

Plumbing for sample line Maintenance Ü ANL maintenance Ü S/ R dispenser syringe replacement

Plumbing for reagent line Maintenance Ü ANL maintenance Ü S/ R dispenser syringe replacement

2) The mix sequence is executed and the outer wash water of the mix wash pot is filled using the ANL maintenance

menu.

Air extraction position Menu

Plumbing for mix outer wash Maintenance Ü ANL maintenance Ü Mixing bar replacement

3) The wash nozzle sequence is executed and the wash water is filled using ANL maintenance.

Air extraction position Menu

Plumbing for wash nozzle line Maker maintenance Ü ANL maintenance Ü Replacing Wash Nozzle

Caution

The cuvette may be splashed by water from the nozzle the first time because of air in the tube. In the first

time, detach the nozzle portion necessarily and accept by the container.

Once the air has been extracted, replace the nozzle and check the wash condition by discharging the real

wash water into the cuvette.

4) The probe wash sequence is executed and the wash water is filled using ANL DIAG.

Air extraction position Menu

Plumbing for clot detection sensor Maker maintenance Ü ANL DIAG Ü Probe wash

If the water test (analysis test) is executed without the air having been completely removed the

data may not be reliable. Be sure to extract all the air.

n Processing the heater line in the JD portion

1) If the air has been extracted, turn off the power.

2) Execute the processing for the heater line of the JD portion described on page 6-1.

Operation check7-7

0200

Page 4: BC 589 - Olympus

M514 SET UP MANUAL

st08_01.doc

n Executing W1

1) If processing of the heater line of the JD portion has been completed, turn on the instrument again.

2) When the instrument starts, execute W1.

* For the W1 action, check the wash water discharge and aspiration. (It is easy to check if the cover of the GA

portion is detached.)

n Executing photocal

1) If W1 has been completed, execute photocal by closing the GA portion cover and the main body cover.

2) If photocal has been completed, check the cuvette error.

If the average value of the check range is 0.03, there is no cuvette error.

3) If a cuvette error has occurred, check the applicable cuvette and execute photocal again after cleaning or

replacing it.

4) Keep executing until there is no cuvette error.

8-1Test running

Page 5: BC 589 - Olympus

M514 SET UP MANUAL

st08_01.doc

n Water test

l If W1 and photocal have been completed, execute the water test as follows and check the photometry line

performance.

1) Enter the parameters as follows.

2) Prepare degassed water (if it is difficult to obtain, use DI water instead) with P8P (triton 100X) to 0.1%. (88

samples x 4 tests).

NAME 340nm 380nm 480nm 600nm

CH R1=2 R1=4 R1=6 R1=8

Amount for sample dispensing 2 2 2 2

Amount for sample dilution 0 0 0 0

Force- multiplier of

sample prior-diluent

1 1 1 1

Amount for R1 dispensing 50 50 50 50

Amount for R1 dilution 100 100 100 100

Amount for R2 dispensing 0 0 0 0

Amount for R2 dilution 0 0 0 0

Measurement wavelength (main) 340nm 380nm 480nm 600nm

Measurement wavelength (sub) 410nm 520nm 660nm 800nm

Analysis method END 1 END 1 END 1 END 1

Reaction slope + + + +

Measurement point 1 1st 1 1 1 1

Measurement point 1 Lst 27 27 27 27

CAL Type MB MB MB MB

Factor 10000 10000 10000 10000

* Others : Set the maximum value, etc.

* Name, CH, and the reagent bottle position : Set in position optionally.

3) Set the 88 sample racks in white cups and put DI water in each cup.

4) Put the degassed water with triton (made in item 2) into the reagent bottle, and place one in the refrigerator.

5) The 4th requisition of the 88 samples is registered in normal test requisition in the routine menu.

6) Start analysis.

7) The data must satisfy the standard below.

Standard The range of 340/410nm, 380/520nm : less than 15 for all channels.

The range of 480/660nm, 600/800nm : less than 10 for all channels.

n Test running

Be sure to perform a water test to check the conforming standard if the photometry and the dispenser system are running

correctly.

Test running8-2

Page 6: BC 589 - Olympus

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