basic and molecular genetics...2020/10/09 · genetic mapping lab, day 2, group i+ii (pick virgins)...

Schedule Basic and Molecular Genetics, 3MB035, 5MO104

Version: 2020-10-09

(2)

BASIC AND MOLECULAR GENETICS

Course code: 3MB035, 5MO104

Time: 2020-11-02 – 2020-01-17

Lecture halls:

Hörsal B, Building TBA; Berga, Building TBA; Betula, Building TBA; Hörsal 933, Building TBA.

Labs: Red+Green labs and annexes, Building 6L (floor -1)

On-line activities Zoom ID will be sent out later

Course requirements:

1. All lab work, student presentations and the ethics lecture /discussion are mandatory. Attendance to lectures and the calculation workshops is not mandatory but recommended. If you FEEL SICK, STAY HOME and inform the course leader as soon as possible. 2. Information will be in lectures and the text book.

Literature, Text book: Hartwell et al.: GENETICS, from genes to genomes. 5th and 6th edition

Examination: Written exam: TBA Re-exam: TBA

Course leader: Yuri Schwartz [email protected]

Course administrator: Lina Helgesson [email protected]

Assistants:

Alexander Glotov [email protected] Ulf Persson [email protected] Moa Lundkvist [email protected]

Lecturers

Jan Larsson (JL) Molecular Biology Anna Lena Chabes (AC) Medical Biochemistry and Biophysics Monica Holmberg (MH) Medical Biosciences Yuri Schwartz (YS) Molecular Biology Juan Barrasa (JB) Molecular Biology Francesca Aguilo (FA) Molecular Biology Anna Rosén (AR) Epidemiology and Global Health Kalle Grill (KG) Philosophy

mailto:[email protected]





Schedule Basic and Molecular Genetics, 3MB035, 5MO104

Version: 2020-10-09

(2)

EXPECTED LEARNING OUTCOMES OF THE COURSE After the course the student:

• Should understand basic genetics and have gained an understanding of how inheritance is governed by molecular mechanisms.

• Should be able to explain how inheritance is studied at the cellular and organismal level and how allele frequencies vary in populations depending on various factors.

• Should be able to examine, interpret, and statistically evaluate the results obtained.

• Should be familiar with the study, presentation and discussion of original scientific articles.

• Should have acquired the tools to reflect on ethical problems in the application of genetic knowledge.

Fall HT20 THIS IS PRELIMINARY SCHEDULE SUBJECT TO CHANGES

Date Time Activity Location

WEEK 45

09.00 – 09.45Roll call + Course Introduction

(YS)Zoom

10.00 – 12.00

Introduction of project works:

Genes and inherited syndromes

(YS) Zoom

13.00 – 17.00

Introduction and planning:

Genetic mapping Lab (Course

assistants) Zoom

Self study (Hartwell: Chapter 2, 3)

12.00 – 14.00 Principles of Heredity I (YS)

14:15– 16.15 Principles of Heredity II (YS)

Self study (Hartwell Chapter 4)

09.00 – 11.00Chromosome theory of

inheritance (YS)Berga

12.00 - 14.00Genetic mapping Lab, Day 1,

Group I (Learn fly pushing)


Group II (Learn fly pushing)

09.00 – 11.00 Probability and genetics (YS) Berga


Group III (Learn fly pushing)


Group IV (Learn fly pushing)



Group V (Learn fly pushing)


Group VI (Learn fly pushing)

13.00 – 15.00Linkage, recombination & gene

mapping I (JL)Hörsal B


WEEK 46

09.00 – 11.00Linkage, recombination & gene

mapping II (JL)Berga

12.00 – 16.00Mutation Mapping Lab (MML).

Introduction (assistants)Zoom


8.00 – 8.25Genetic mapping Lab, Day 2,

Group I+II, (remove old flies)


Group III+IV (remove old flies)

09.00 – 11.00 Gene expression (FA) Betula


Group I+II (pick virgins)


Group III+IV (pick virgins)


Hörsal B

Red lab

Mon 9/11

Tue 10/11

Red Lab

Red Lab

Thu 5/11 Red lab

Fri 6/11

Mon 2/11

wed 4/11

Red lab

Tue 3/11


Group I+II, (remove old flies)


Group III+IV (remove old flies)


Group I+II (pick virgins)Red Lab


Group III+IV (pick virgins)

14.30 – 16.30 Gene regulation I (YS) Betula



Group V+VI (remove old flies)

09.00 – 11.00 Gene regulation II (YS) Betula


Group V+VI (pick virgins)Red Lab



Group V+VI (remove old flies)Red Lab

9.00 – 11.00 Genetics of development I (YS) Betula


Group V+VI (pick virgins)Red Lab


WEEK 47

09.00 - 11.00 Genetics of development II (YS) Betula


Group I+II (set up P cross)


Group III+IV (set up P cross)


Group V+VI (set up P cross)


09.00 – 12.00Recombinant DNA techniques

(JB)Berga

13.00 – 15.00Calculation workshop A Groups I,

II , III (problems 1-14)

15.00 – 17.00Calculation workshop A Groups

IV, V, VI (problems 1-14)


09.00 – 11.00PCR and DNA sequencing

techniques (JB)Berga


Group I+II (transfer P cross)


Group III+IV (transfer P cross)


Group V+VI (transfer P cross)


09.00 – 11.00 Mutations (YS) Berga

12.00 – 16.00 MML Group A (Day 1) Red lab + Green Lab


08.00 – 12.00 MML Group B (Day 1)

13.00 – 17.00 MML Group C (Day 1)

Red Lab

Thu 19/11

Fri 20/11 Red lab + Green Lab

Mon 16/11 Red Lab

Tue 17/11Zoom

Wed 18/11

Fri 13/11

Red Lab

Thu 12/11

Wed 11/11

WEEK 48

09.00 – 11.00 Genomes (JL) Berga


Group I+II (remove P parents)


Group III+IV (remove P parents)


Group V+VI (remove P parents)

14.30 – 15.30Life Science program meeting

(only Life Science students)TBA



09.00 – 17.00 MML Group B (Day 2) Red lab + Green Lab


Thu 26/11 09.00 – 17.00 MML Group C (Day 2) Red lab + Green Lab


15.00 – 17.00 Population genetics I (JL) Betula

WEEK 49

09.00 – 11.00 Population genetics II (JL) Hörsal B

12.00 – 17.00 MML Group B (Day 3) Red lab + Green Lab

Self study (Hartwell Chapter

11,22)

Genetic mapping Lab, Day 7,

Group IV (analyze F1,

Setup F1 cross)


Group III (analyze F1,

Setup F1 cross)


Group II (analyze F1,

Setup F1 cross)


Group I (analyze F1,

Setup F1 cross)

09.00 – 11.00 DNA replication and repair (AC) Berga

12.00 – 17.00 MML Group C (Day 3) Red lab + Green Lab


Group IV (transfer F1 cross)


Group III (transfer F1 cross)


Group II (transfer F1 cross)


Group I (transfer F1 cross)

12.00 – 17.00MML (time to repeat

experiments)Red lab + Red anex


09.00 – 11.00Localization of disease genes

(MH)Betula

12.00 – 17.00MML: Sequence analysis

workshop (all groups)Zoom

Fri 4/12

Fri 27/11

Mon 23/11Red lab

Tue 24/11

Wed 25/11

Mon 30/11

Red lab

15.00 – 17.00

Wed 2/12

Thu 3/12

Tue 1/12

09.00 – 11.00

Red Lab

11.00 – 13.00

13.00 – 15.00

WEEK 50

09.00 – 11.00Calculation workshop B Group I,

II, III (problems 15-29)

12.00 – 14.00Calculation workshop B Group IV,

V, VI (problems 15-29)


Group I (remove F1 parents)


Group II (remove F1 parents)


Group III (remove F1 parents)


Group IV (remove F1 parents)

09:00-12:00Ethics in Genetics (group 1, see

separate schedule) (AR, KG)Betula

13:00-16:00Ethics in Genetics (group 2, see

separate schedule) (AR, KG)Hörsal 933

09.00 – 11.00 Genome editing techniques (YS) Hörsal B

12.00 – 14.00Calculation workshop C Group I,


14.00 – 16.00Calculation workshop C Group IV,


Thu 10/12 09.00 – 17.00 MML (sequence analysis) Zoom

09.00 – 12.00 MML (sequence analysis) Zoom


experiments), Group I, IIRed lab + Red Anex

13.00 – 16.00 MML (sequence analysis) Zoom


experiments)Red lab + Red Anex

WEEK 51

Zoom

Red lab + Red Anex

13.00 – 15.00Calculation workshop D Group I,


15.00 – 17.00Calculation workshop D Group IV,



Group I (analyze F2)


Group II (analyze F2)


Group III (analyze F2)


Group IV (analyze F2)

09.00 – 11.00 MML (result defense)

12.00 – 14.00Calculation workshop E Group I,


14.00 – 16.00Calculation workshop E Group IV,




MML (sequence analysis)/(time

to repeat experiments)

Tue 15/12 Red Lab

Thu 17/12 Red Lab

Fri 11/12

Mon 14/12

09.00 – 12.00

Tue 8/12

Wed 9/12Zoom

Mon 7/12

Zoom

Wed 16/12 Zoom

Zoom

Red Anex







09.00 – 11.00 Self-assessment Quiz Betula

12.00 – 14.00Calculation workshop F Group I,


14.00 – 16.00Calculation workshop F Group IV,


WEEK 52









Thu 22/12 09.00 – 17.00 MML (result defense) Zoom

Wed 23/12 09.00 – 12.00Self study (Project work/Lab

report/preparation for exam)

WEEK 53

Mon 28/12 09.00 – 17.00Self study (Project work/Lab


Tue 29/12 09.00 – 17.00Self study (Project work/Lab


Wed 30/12 09.00 – 17.00Self study (Project work/Lab


WEEK 1

Mon 4/1 09.00 – 17.00Self study (Project work/Lab


Thu 7/1 09.00 – 17.00 MML (result defense) Zoom


project presentations

(see separate schedule)

WEEK 2







Wed 13/1 Lab reports (deadline)

09.00 – 11.00Questions & Answers session (YS,

JL)Betula

Preparation for exam/course

evaluation

Fri 15/1 TBA EXAM.

Tue 12/1 09.00 – 17.00 Betula

Thu 14/1

Mon 11/1 09.00 – 17.00 Hörsal B

Fri 8/1 09.00 – 17.00 Hörsal B

Mon 21/12 Red Lab

Thu 17/12 Red Lab

ZoomFri 18/12

basic and molecular genetics...2020/10/09 · genetic mapping lab, day 2, group i+ii (pick virgins)...

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