bài 3 (1)

7/23/2019 Bài 3 (1)

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BỘ MÔN: THỰC HÀNH XỬ LÝ SỐ LIỆU THỰC NGHIỆM

TRONG PHÒNG THÍ NGHIỆMBáo Cáo Phâ T!"h #$ L%&'

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6

7

8

9

10

Mẫu

2"ng ,u1t

W ':

: DYZ'E[eJKKEY7 \r [7J7 ƒ9 ='> " 9%n9 † ̂ h9 "_ =` ^há" Q%&J ƒ9 ='>† ^h9 "_ =` ^há" Q%& 9%n9 1] K hw9 % "_ =` ^há" Q%& 9%n9 e]2J 6a wx"h%&' k'8 n h> "hb 9%n9 K a Dhy Q% ;9 ;% ;o9 =8 „'>

GIỐNG L–• Nƒ9 ='> 1 4]77 " vY 1]77 K ]37 " vY 1]12

e 2]37 vY 1]12 2 2]7 Q vY 7]2

Phd d"

\ 9;o'D["?1]1]1]1]1]K]K]K]K]K]e]e]e]e]e]2]2]2]2]2A

\ 9;o'D[=Jf"o;?9;o'DA

\ 9%o9["?]4]3]3]]]17]4]]]]]2]e]3]]2]]2]3A

\ V[VJf;FE?9;o'D]9%o9A

\ "h?VA


7/23/2019 Bài 3 (1)


9%o9] 9;o'D

ThE fooj%9 oQÊ"= ;E F=ÊV f;oF V ?Do= [ eA:

9%o9] 9;o'D

\ =%=[F?9%o99;o'DA

\ oZ?=%=A

•=%= of (;%"E TQE

RE=Do=E: 9%o9


9;o'D e 2J 1J1e 13J1 eJKKEY7 ŠŠŠ

RE=%V'= 13 14JK7 1J74

YYY


\ ;E=[oZ?9%o99;o'DA

\ T'ÊHS#?;E=A



i%: oZ?fo;F' [ 9%o9 9;o'DA

9;o'D

7/23/2019 Bài 3 (1)


V%ff j; 'D; D Vˆ

KY1 1J3 Y7JK4373K eJ24373K 7J1711

eY1 YKJ2 Y2JK4373K Y7JKe7e 7J717Ke11

2Y1 YKJK Y2J74373K Y7JeKe7e 7J71eK

eYK Y2J7 YJ4373K YKJ1Ke7e 7J7777KK

2YK YeJ YJ34373K Y1JKe7e 7J77712

2Ye 7JK Y1J34373K KJ74373K 7J4477

Bài 13:

B89: N9 EF~F =` h y% hcF wx9 Z%F%"

9 7 7J7 7J1 7J1 7JK 7JK 7JehcFwx9 ZF

2J7K 43J2e J3 7J4 eJ3 JKe 3J212Jee 4J32 7JK KJ1 2J2K 4J1K 4J3KJ12 4Je1 4J2 1J3 2J 4J1 J4e

Q 2Je 4J1K333334

J7e334

1J42 2JeKeee

3J4eee

4J3334

V" KJ7e77

2

1J747311

1JeeK4

2

7J77e

7J41

2

1Je441

11

1J1377

12

0.020.040.060.080.0

100.0120.0

2ồng độ ch3 phẩm (%+45)

*6m 7$8ng +itamin 9

7/23/2019 Bài 3 (1)


B%' : N9 EF~F =` h y% hcF wx9 Z%F%"

W ':

DYZ'E[KJeŠ17Y1qr[7J7 hcF wx9 Z%F%C ?F9z9A "á" 9 "h Dh‚F "_ =`^há" Q%& a hcF wx9 (%F% C h%&' k'8 "hb 9 c 7J1@

N9 E~FE HcF wx9 Z%F% C 7 2Je v‰ KJ7e

7J7 4J1K v‰ 1J1 7J1 J7e Q v‰ 1Jee 7J1 1J42 Q]" v‰ 7J7 oJK 2JeK"]V v‰ 7J4K

7JK 3J4V v‰ 1Je 7Je 4J4V v‰ 1J13

Phd d":

\9;o'D["?7]7]7]7J7]7J7]7J7]7J1]7J1]7J1]7J1]7J1]7J1]7JK]7JK]7JK]7JK]7JK]7JK]7Je]7Je]7JeA

\ 9;o'D[=Jf"o;?9;o'DA\ZF["?2J7K]2Jee]KJ12]43J2e]4J32]4Je1]J3]7JK]4J2]7J4]KJ1]1J3]eJ3]2J2K]2J]JKe]4J1K] 4J1]3J21]4J3]J4eA

\ V[VJf;FE?9;o'D]ZFA

\ "h?VA


9;o'D] ZF

\ =%=qYF?ZF9;o'DA

7/23/2019 Bài 3 (1)


\ oZ?=%=A

•=%= of (;%"E TQE

RE=Do=E: ZF


9;o'D 3 12JK 32J72 242J43 KJeEY1 ŠŠŠ

RE=%V'= 12 KJ 1JK

YYY


\ ;E=[oZ?ZF9;o'DA

\ T'^EHS#?;E=A



i%: oZ?fo;F' [ ZF 9;o'DA

9;o'D

V%ff j; 'D; D Vˆ

7J7Y7 KJK33334 K2Je1 eKJ741 7J7777777

7J1Y7 eJK733334 eJ221 2KJ341 7J7777777

7J1Y7 21J177777 eJ122 2J34111 7J7777777

7JKY7 22J2eeeee 27J4eK1KK 2JK222 7J7777777

7JKY7 23JKeeeee 2eJ13K1KK 7J3222 7J7777777

7JeY7 24J4e33334 2eJ41 1J241 7J7777777

7/23/2019 Bài 3 (1)


7J1Y7J7 17J177777 4J122 12J34111 7J777771

7J1Y7J7 1eJ31eeeee JK1KK 14Je4222 7J7777771

7JKY7J7 13J133334 1KJ2e1 1J41 7J7777777

7JKY7J7 1J3K33334 12J31 KKJe41 7J7777777

7JeY7J7 1J2277777 1J342 KeJK7111 7J7777777

7J1Y7J1 KJ47eeeee Y1J7414 3J23222 7JK23ee

7JKY7J1 JK33334 1JK1 J7241 7J772733

7JKY7J1 4J4133334 eJ1 11J2441 7J7777K

7JeY7J1 Je77777 2J432 1KJK111 7J7777eK

7JKY7J1 KJeeeee Y1J14414 3Je2222 7JKK212

7JKY7J1 J71eeeee 1JKK1KK J44222 7J773e3eK

7JeY7J1 JK33334 KJ731 J41 7J77147K

7JKY7JK KJ2e77777 Y1Jee111K 3J1111 7Je73eK

7JeY7JK eJK2eeeee Y7J1414 4J77222 7J11e1713

7JeY7JK 7J1eeeee YKJ2414 2J4222 7J4e37

Bài 14:

WL:

DYZ'E [KJKEY13qr[7J7 "_ =` h y% hEo — 9h˜ hn9 ^E

Phd d":

\ 9wt%["?K2]12]e34]147]K47]2A

\ •[F;%„? 9wt%] ;oj[K]"o[e] Q;oj[T] V%FFE= [ %=?"?™9 1J Y e™]™9 7JY1™A] "?™V1™] ™VK™] ™Ve™AAA

\ "h%=kJE=?•A

7/23/2019 Bài 3 (1)


PE;=o“= Ch%Y=k';EV E=

V: •

XY=k';EV [ K2J3] Vf [ K] DYZ'E q KJKEY13

Bài 15:

WL:

DYZ'E [KJKEY13qr[7J7 "_ =` h y% hEo — 9h˜ hn9 ^E

Phd d":

Q%1[F;%„?"?14]1]147]K]]1K]14]e]e]]4]KA];oj[e]"o[2]Q;oj[T]V%FFE=[%=?"?™">' ;†" n™]™">' ;†" Z}™]™">' ;†" ™A]"?™D9 ™]™D9 Q™]™D9 "™]™n% "h9™AAA

\ "h%=kJE=?Q%1A


V: Q%1

XY=k';EV [ 4J1e] Vf [ 3] DYZ'E q KJKEY13

Bài 16:

B89 : 9 E~FE Zc HcF wx9 DhEo

N9E~FE

7 7J7 7J1 7J1 7JK 7JK 7Je

HcFwx9 DhEo

K7Je K2J K4J2 KJ KJ e7J1 KJK1J KJ1 K4J KJK e7J e1J4 eKJ1KeJ3 K4JK KJe e7J3 eKJ2 e7J e7J3

TB K1J KJ3 K4J3333334

KJ2eeeeeee

e7J e7J43333334

e7J3333334

6LC 1J347eKe7

1J2144223

7J27K24

1J73K3433K

1Je2e3K27

7JeK3332

1J1Ke17K

7/23/2019 Bài 3 (1)


0.05.0

10.015.0

20.025.030.035.0

2ồng độ enzyme (%)

*6m 7$8ng phen'7

B%' : 8h hw9 " 9 E~FE ‡ h%&' ='> ;!"h

W ':

PYZ'E [ 1J73KJ17Y3q α =0.05 hcF wx9 DhEo "á" 9 E~FE "_ =` ^há" Q%&

N9 E~FE?@A H%&' ='>?@A7 K1J7 1J34

7J7 KJ37 Q 1J2K7J1 K4J7 Q" 7J27J1 KJ27" 1J747JK e7J7" 1Je7JK e7J44" 7Je7Je e7J4" 1J1K

Phd Ld"

9;o'D["?7]7]7]7J7]7J7]7J7]7J1]7J1]7J1]7J1]7J1]7J1]7JK]7JK]7JK]7JK]7JK]7JK]7Je]7Je]7JeA

\ 9;o \ Dh["?K7Je]K1J]KeJ3]K2J]KJ1]K4JK]K4J2]K4J]KJe]KJ]KJK]e7J3]KJ]e7J]eKJ2]e7J1]e1J4]e7J]KJ]eKJ1]e7J3A

7/23/2019 Bài 3 (1)


\ V[VJf;FE?9;o'D]DhA

\ "h?VA


9;o'D] Dh

\ =%=[F?Dh9;o'DA

\ oZ?=%=A

•=%= of (;%"E TQE

RE=Do=E: Dh


9;o'D 3 K11J711 eJ13 K2Jee 1J73KEY73 ŠŠŠ

RE=%V'= 12 1JK4 1J213

YYY


\ ;E=[oZ?Dh9;o'DA

\ T'^EHS#?;E=A



i%: oZ?fo;F' [ Dh 9;o'DA

9;o'D

7/23/2019 Bài 3 (1)


V%ff j; 'D; D Vˆ

7J7Y7 eJ7777777 7J2K143K 4J114K2 7J7K721

7J1Y7 3J73333334 KJ422K Je227 7J777ee

7J1Y7 4J3eeeeeee 2Je173 17J114 7J7777K43

7JKY7 J17777777 J4K143K 1KJ214K2 7J77777e

7JKY7 J3333334 J322K 1KJK227 7J777772K

7JeY7 J73333334 J422K 1KJe227 7J77777e4

7J1Y7J7 KJK3333334 Y1J71141 J227 7JK22e

7J1Y7J7 eJeeeeeee 7J173 4J1114 7J711e7

7JKY7J7 Je7777777 1JK143K J314K2 7J771K44e

7JKY7J7 J13333334 1J22K J2227 7J7713KK

7JeY7J7 JK3333334 1J22K J227 7J771e4

7J1Y7J1 1J3333334 Y1J41141 2J227 7J3443K17

7JKY7J1 eJ7eeeeeee Y7JK2272 3Je114 7J7e4

7JKY7J1 KJ7777777 Y7J214Ke 3JK14K2 7J174733

7JeY7J1 eJ77777777 Y7Je14Ke 3Je14K2 7J734

7JKY7J1 1J23333334 Y1J1141 2J4227 7J4eK

7JKY7J1 1Jeeeeeeee Y1J2272 2J3114 7J74K13e

7JeY7J1 1J2eeeeeee Y1J2272 2J4114 7J42KK3

7JKY7JK Y7J1eeeeeee YeJ21141 eJ1227 7JK

7JeY7JK Y7J7eeeeeee YeJe1141 eJK227 1J7777777

7JeY7JK 7J17777777 YeJK14Ke eJ214K2 7J

Bài 17:

B89: "h> wx9 "â ;9 ;‡ e o% >

7/23/2019 Bài 3 (1)


Ch> wx9 "â ;9Ty9 =n

X>' Tn

Lo% >

• 27 147 K17

B 7 17 Ke7C 37 17 K17

Ty9 =n 17 77 37

"_ DYZ'E [ 7J7413\r[7J7 =n %&' ^h9 "_ =` y%

Phd d":

\ Q%14[F;%„?"?147]27]17]7]17]37A];oj[K]"o[e]Q;oj[TRUA

\ "h%=kJE=?Q%14A


V: Q%14

XY=k';EV [ 13J2] Vf [ K] DYZ'E q KJKEY13Bài 18:

7/23/2019 Bài 3 (1)


0 0.5 1 1.5 2 2.5 3 3.5

0

0.2

0.4

0.6

0.8

1

1.2

1.4

1.6

1.8

2

0.61 1

2ồng độ benzen (g47)

M:t độ đ' ;uang (<m)

W ':

"_ DYZ'E [ 7J7413\r[7J7 ^h9 "_ =` ̂ há" Q%&

Phd d":

\ "[ "?7JK]7J]1J7]1J]KJ7]KJ]eJ7A

\ •M[ "?7JK]7Je4]7J32]7Je]1JKK]1J7]1J7A

\ k[VJf;FE?"]•MA\ F?"•MA

C:

F?fo;F' [ " •MA

CoEff%"%E=:

?IE;"EDA •M

Y7J1e2 1J4e1

7/23/2019 Bài 3 (1)


Bài 19:

B89: C9 ='> =%‡' âF] hcF wx9 Z%F% "

CS 7 17 1 KK K3e e77

(TM C 7J 3Je 177J4 17eJ3 177J3 4J42J4 J 171JK 17KJ2 17KJ1 J21J2 4J J3 17eJ J JK

TB 7J 3J3 177J 17eJe 177J4 J2#" 7J 7Je 7JK 7J4 1Je1 7J4

0 150 188 225 263 300

0.00

20.00

40.00

60.00

80.00

100.00

120.00

9=ng ,u1t ,i>u ?m (@)

*6m 7$8ng +itamin 9

B% : šh hw9 " "9 ='> ‡ hcF wx9 Z%F% "

W ':

Y PYZ'E [ KJ4EY13 q α =0.05 šh hw9 " "9 ='> ‡ hcF wx9 Z%F% "

c "_ h y% J T "hb "9 ='> KK Z€ "9 ='> c "_ =` ^há" Q%& hâ Zc "_

9%á ;< ;'9 Q€h hcF wx9 Z%F% C { h>J

CÔNG SU*T SI+U ›M HÀM LœNG (IT•MIN C7 7Je v‰ 7J

17 3J4 Q v‰ 7Je

7/23/2019 Bài 3 (1)


1 177J " v‰ 7JK KK 17eJe V v‰ 7J7 K3e 177J4e" v‰ 1Je7 e77 J2e Q] " v‰ 7J4

Phd d"

9;o'D["?7]7]7]17]17]17]1]1]1]KK]KK]KK]K3e]K3e]K3e]e77]e77]e77A

\ZF"["?7J]2J4]1J2]3Je]J]4J]177J4]171JK]J3]17eJ3]17KJ2]17eJ]177J3]17KJ1]J]4J4]J2]JKA

\ 9;o'D[ =Jf"o;?9;o'DA

\ V[ VJf;FE?9;o'D]ZF"A

\ "h?VA


9;o'D] ZF"


9;o'D

\ =%=[F?ZF"9;o'DA

\ oZ?=%=A

•=%= of (;%"E TQE

7/23/2019 Bài 3 (1)


RE=Do=E: ZF"


9;o'D 3141J 1Ke2Je 123JK KJ4EY13 ŠŠŠ

RE=%V'= 1K 17J7 7Je

YYY


\ ;E=[oZ?ZF"9;o'DA

\ T'^EHS#?;E=A



i%: oZ?fo;F' [ ZF" 9;o'DA

9;o'D

V%ff j; 'D; D Vˆ

17Y7 23J7eeeeee 2eJee144 2JeK42 7J7777777

1Y7 2J333334 24J234K17 KJ2337Kee 7J7777777

KKY7 KJ4333334 7JK34K17 JK337Kee 7J7777777

K3eY7 7JK777777 24J4772e KJ32133 7J7777777

e77Y7 24J777777 2J2772e 7Je2133 7J7777777

1Y17 eJeeeeee 1J2ee144 3J2eK42 7J77K72e

KKY17 3J4eeeeee 2JKee144 JKeK42 7J77771KK

K3eY17 2J1333334 1J334K17 3J3337Kee 7J771K27

e77Y17 1J333334 Y7J3eK427 2Je337Kee 7J1221

7/23/2019 Bài 3 (1)


KKY1 KJ777777 7Je772e JK2133 7J7Ke11

K3eY1 7JKeeeeee YKJK337Ke KJ4eK42 7J24

e77Y1 YKJ7333334 Y2J337Ke 7J2eK42 7J1K222

K3eYKK YKJ333334 YJ7337Ke Y7J734K171 7J72K32e

e77YKK Y2J333334 Y4Je337Ke YKJe34K171 7J777e7

e77YK3e YKJe777777 Y2J4214 7J12133 7J747e1

Bài 20:

B89: Phwt9 DháD ƒ9 h%&' ='> h' h% V<"h "h%

E~FE S_9 =%‡'âF

W hxD ~FE Zc =_9=%‡' âF

7J34 3J1e J124JKe 4eJ 4J11J2K 41JK4 J3

TB 7J11 41J2K 4J116LC 1J34 KJe3 1JK4

0.010.020.030.040.050.060.070.080.090.0

100.0

dAch chi3t (%)B

6 h<: hiệu suất thu hồi enzyme(1YQy ='9 E~FE…KYS_9 =%‡' âF…eYW hxD ~FE

Zc =_9 =%‡' âFA

7/23/2019 Bài 3 (1)


W ':

PYZ'E [7J7771eee q α =0.05 "á" Dhwt9 DháD h' h% V<"h "h% "_ =` h y%J 6a

h%&' k'8 "o h> Zc h%&' ='> h' h% V<"h "h% "o h> c =s Vd9 Dhwt9 DháD ^

hxD =_9 =%‡' âF Zc =s Vd9 E~FEJ

Phwt9 DháD H%&' ='> h' h% V<"h "h%

By ='9 E~FE 7J11 Q vY 1J34

S_9 =%‡' âF 41J2K vY KJe3

W hxD 4J11 " vY 1JK4

Phd d":

\ 9;o'D["?1]1]1]K]K]K]e]e]eA

\ 9;o'D[=Jf"o;?9;o'DA

\ h=["?7J34]4JKe]1J2K]3J1e]4eJ]41JK4]J12]4J1]J3A

\ V[VJf;FE?9;o'D]h=A

\ "h?VA


9;o'D] h=

\ =%=?h=9;o'DA

;;o;: "o'V o f%V f'"%o ™=%=™

\ =%=[F?h=9;o'DA

\ oZ?=%=A

•=%= of (;%"E TQE

7/23/2019 Bài 3 (1)


RE=Do=E: h=


9;o'D K e47J 1J24 J4K 7J7771eee ŠŠŠ

RE=%V'= 3 1J4 eJeK

YYY


\ ;E=[oZ?h=9;o'DA

\ T'^EHS#?;E=A



i%: oZ?fo;F' [ h= 9;o'DA

9;o'D

V%ff j; 'D; D Vˆ

KY1 YJ37777 Y1eJK377K Y2J111 7J77K47e4

eY1 4J773334 KJ2e3 11J4423 7J77473

eYK 1J33334 11J1K3 K7JK3423 7J77717

Bài 21:

B89 : hcF wx9 NOe Y "_ ;o9 K Fm' w{"

STT Ố9 7 Ố9 1 Ố9 K Ố9 e Ố9 2 Mm' 1 Mm' 1 N9 "h'‚

7 K 2 3 7 7

•F 7 7Je3 7J4 1J14 1J3 1J7Ke 1J34

7/23/2019 Bài 3 (1)


W ':

Y Mok'9;'9Q€h"Fm'w{":

´ y=1.023+1.657

2=1.34

?•FA

Y N9%;"_Fm';o9w{": x= ´ y

0.196∗ H ệ số phaloãng

¿ 1.34

0.196∗10=68.4 ?DDFA

0 2 4 6 8

0 . 0

0 . 5

1 . 0

1 . 5

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7/23/2019 Bài 3 (1)


„["?7]1]K]e]2A

\ o9Vo[„ŠK7z17

\ Vok'9["?7]7Je3]7J4]1J14]1J3A

\ VK1[VJf;FE?Vok'9]o9VoA

\ Do?Vok'9o9Vo]D"h[13]„Q[™N9%; ?FA™]Q[™Mok'9?•FA™]='Q[™Mn%k'h&9%$9%;ZcFok'9™A

\ ;E9[F?Vok'9o9VoA

\ Q%E?;E9A

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RE=%V'=:

1 K e 2

Y7J77KK 7J771 7J771 Y7J777K Y7J771K

CoEff%"%E=:

=%FE SVJ ;;o; Z'E P;?\žžA

?IE;"EDA 7J77KK777 7J7713777 1Je4 7JK3e

o9Vo 7J137777 7J777eK33 377J1K 1J7KEY7 ŠŠŠ

YYY


7/23/2019 Bài 3 (1)


RE=%V' =V;V E;;o;: 7J77K733 o e VE9;EE= of f;EEVoF

M'%DE RY=k';EV: 1] •Vˆ'=EV RY=k';EV: 1

iY=%=%": eJ371Ev7 o 1 V e #i] DYZ'E: 1J7KEY7

\ E9EV?]7J]"?™[7J13„ŸR=k';E[1™AA

Bài 22:

W ':

p-v!ue " 0#02886 $0#05 ha h%& "_ =` ^há" h' Zl =n wx9 hc9 "h!h Dh‚F ;w{" Zc=' ^h% h y% "9 9h&

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PE;=o“= Ch%Y=k';EV E= j%h /E=“ "o%'% "o;;E"%o

V: FV

XY=k';EV [ 2J444] Vf [ 1] DYZ'E [ 7J7K3

Bi 23:

B89: M" h!"h " 9wp% %‡' V9 " e =8 Dh‚F ;c

T;c ;' Fá T;c V T;c ̂ hyk'

N9wp%1 3JK 3J3 3J2 N9wp%K 3JK 3Je 3J2 N9wp%e 3J 3J4 3J

7/23/2019 Bài 3 (1)


N9wp%2 3JK 3J3 3 N9wp% 3J2 3J4 3J3 N9wp%3 3J 4 3J2 N9wp%4 3J 3J 3J N9wp% 3JK 3J 3J1 N9wp% J 3J2 3Je N9wp%17 3JK 3J 3JTB 3JK4 3J33 3J26LC 7JKK 7JKe 7JKe

T +: m- T ;ứ+ T <h= >:+

0

1

2

3

4

56

7

8

9

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.i-m

6 h<: ha h%& F" ‡' h!"h n% Z{% "á" o% ;c

W ':

PYZ'E[7J77K1K q α =0.05 M" h!"h " 9wp% %‡' V9 c "_ =` h y%JS8

Dh‚F ;c V wx" 9wp% %‡' V9 ‡' h!"h h>J

%&'i tà )*+ , y.u th/+h

T;c ;' Fá 3JKQ vY 7JK2

T;c V 3J47" vY 7JK1

T;c ̂hy k' 3J27 vY 7JK3

7/23/2019 Bài 3 (1)


Phd d":

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bài 3 (1)

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