Avon Longitudinal Study of Parents and Children (ALSPAC)

I4C Barcelona, Spain 2011

Update: Progress to date

Cohort summary Years of recruitment – 1990-2 in pregnancy

Number of live births at baseline 14062

Follow up status currently being contacted at

age 20

Acknowledgements

We are extremely grateful to all the families who took part in this study, the midwives for their help in recruiting them, and the whole ALSPAC team, which includes interviewers, computer and laboratory technicians, clerical workers, research scientists, volunteers, managers, receptionists and nurses. The UK Medical Research Council, the Wellcome Trust and the University of Bristol currently provide core support for ALSPAC.

For further details see the website :

www. Bristol.ac.uk/alspac

Please note that the numbers of samples

available was correct at 23.2.10.

Jean Golding and Colleagues, Centre for Child and Adolescent Health, School for Social and Community Medicine, University of Bristol, Bristol, UK

ENVIRONMENTALSAMPLES

COLLECTED( YES/NO)

TIMING OF SAMPLE COLLECTION(weeks gestation / months of age)

LOCATION OF SAMPLE COLLECTION(e.g. child’s bedroom)

CONDITIONS OF STORAGE MONTHS SINCE

STORED

TOTAL NUMBER OF SAMPLES

COLLECTED

NUMBER OF SAMPLES

AVAILABLE SEPT 2011

Prenatal First year of life

Indoor air sample Y (a) study homes monitored over a 12 month period

(b) -

(a) Approximately first 6 months

(b) 1st year

(a) Not stored but analysed for NO2, formaldehyde, total VOCs, undecane, bacteria,

(b) NO2 indoors and out

DNA (a) 150 homes, manysampling points per home(a) 1200 indoors, 500 of these

outdoors

DNA

Dwelling dust collection N

Soil sample N

Drinking water N

Hair sample from pets N

Geographic Information Systems (GIS): Describe type of data collected, source of data, other relevant information.

The study postcodes have been used for a variety of projects including the mapping of accidents and distance from overhead power lines.

Population sample

Update: Primary outcomes

Cases of childhood cancer up until the age of 14

have been linked.

BIOLOGICAL

SAMPLES

TIMING ( Indicate YES (Y) or NO (N) AND

weeks gestation/months of age)

CONDITIONS OF STORAGE

MONTHS SINCE

STORED

BRIEF DESCRIPTION OF

COLLECTION METHOD

TOTAL NUMBER OF

SAMPLES COLLECTED

NUMBER OF SAMPLES AVAILABLE

SEPT 2011

Prenatal (Mother)

At birth (Mother/Child)

First year of life(Child)

No of individuals for whom samples are available

Blood Y See Cord blood below

Collected but no samples left

(a) Prenatal: Whole blood EDTA; -20(b) Red cells EDTA; -70(c) Freeze dried plasma; room temperature(d) Plasma; -20/-70(e) Red cells hep; -70(f) White cells hep; -70

240 Collected by midwife during clinical examination and sent to ALSPAC laboratories

(a) 7494 (b) 1613(c) 1945 x 0.5ml; 339 x 1ml(d) 8103 x 1ml [up to 6 aliquots]; 524 x 0.5ml [up to 5

aliquots](e) 2488 (f)2629

Serum Y N Y 240 (a) As above stored at -20(b) Collected from what was left after AFP test (c) Collected from sample stored for rubella testing

(a) 7153 x 1ml [up to 3 aliquots](b) 4466 x 0.5-1ml [up to 3 aliquots](c) 5605 x 0.5 -1ml

Urine Y N N 240 Collected as a midstream specimen and sent to the ALSPAC laboratories where it was aliquoted and stored at -20; up to 5 aliquots per woman.

10939 mothers

Saliva N N N

Buccal swab N N N

Vaginal swab N N N

Cord blood - Y N (a) red cells EDTA at -70; (b) freeze dried plasma at room temp(c) plasma at -70; (d) red cells hep at -70; (e) serum at -20;

240 (a) 263(b) 144 x 1ml and 1898 at 0.5ml(c) 4976 x 1ml, 1594 x 0.5ml.(d) 1725 (e) 6665 x 1ml

Blood spots Y Y N Stored -20 240 (a) Maternal antenatal in heparin(b) Cord blood in heparin

(a) Up to 10 spots for 5687 women(b) Up to 5 spots per child for 9695 children.

Placenta - Y - (a) The whole placenta stored in formalin at room temperature;

(b) Umbilical cord stored at -20

240 (a) Collected by midwives at delivery and put straightinto formalin(a) Umbilical cord: immediately frozen but laterthawed and sliced into 4 aliquots, then stored at -20

(a) 8933(b) 6283

Meconium - N N

Hair sample N N Y Stored at room temp 5076 5076

Nail sample (Toes) N N Y Stored at room temp 4945 4945

Breast milk - N N

Milk teeth N N Y Stored at -20 4168 Up to 4 teeth per child 4168