avin tamsir, jeffrey j. tabor & christopher a. voight
TRANSCRIPT
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Robust multicellular computing using genetically encoded NOR
gates and chemical ‘wires’Avin Tamsir, Jeffrey J. Tabor & Christopher A.
Voight
Presented by Colin Reisterer
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LogicGates
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Cells as computational devices◦ Input-Output◦ Interpreting
environmental cues◦ Logic decisions: rest or
replicate? Multicellular approach
◦ Compartmentalize simple logic gates into different colonies
◦ Colonies can be arranged on plate in defined pattern to achieve circuit.
Multicellular computing
diffusion time∝ distance2
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Simple single cell logic gates
PBAD: Activated by Ara
PTet: Activated by aTc
NOR gate: CI-repressor gene in OR gate, YFP under control of CI-repressible promoter
Using promoters as input gives modularity
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Four other combinations of promoters were tested
Incorporated PLas promoter◦ Responds to 3OC12-
HSL input
Demonstrated modularity of logic gates
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Implemented XOR gate from 3 NOR gates and buffer gate
Multicellular XOR Gate
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Construction of 16 2-input gates No circuits
required genetic manipulation
Results analyzed using flow cytometry
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Advantages:◦ Wide dynamic
range◦ Low variability
between test Disadvantages
◦ Time interval necessary between colony spotting
◦ Applicability?
Discussion of Results
“Reducing the interval from 12 to 6 hours produces an equivalent XOR behaviour. However, when all four XOR colonies were spotted at the same time (0 hours), the quality of the logic function is reduced significantly.”
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Thanks!
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Supplementary Info