automated handheld cell counting - scepter technology - emd millipore
DESCRIPTION
The first and only handheld device to bring consistency in cell counting right to the culture hood is here: the Scepter automated cell counter.TRANSCRIPT
Automated Handheld Cell Counting
Scepter™ Technology
PINPOINT ACCURACY
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THE NEW, MORE ACCURATE WAY TO COUNT CELLS The first and only device to bring consistency in cell counting right to the culture hood is here: the Scepter handheld, automated cell counter.
While other automated counters consume bench space and
rely on object recognition software, manual focusing, and
clumsy loading chambers, the Scepter cell counter provides
true automation without the error that accompanies
vision-based systems.
With its microfabricated, precision-engineered sensor, the Scepter
cell counter does all the work and delivers accurate
and reliable cell counts in less than 20 seconds.
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+ -
The Gold Standard Counter: Smaller than Ever.The Scepter cell counter uses the Coulter principle of impedance-based particle detection to reliably and accurately count every cell in your sample.
Particles are detected by Ohm’s Law V=IR(V=voltage, I=current, and R=resistance)
• Precise volumes are drawn into the Scepter sensor
• As cells flow through the aperture in the sensor, resistance increases. This increase in resistance causes a subsequent increase in voltage.
• Voltage changes are recorded as spikes with each passing cell.
• Spikes of the same size are bucketed into a histogram and counted. This histogram gives you quantitative data on cell morphology that can be used to examine the
quality and health of your cell culture.
60%
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Perc
ent
CV
Scepter cell counter Vision-based counter Hemocytometer
= Average %CV
= Coulter Counter %CV
Figure 1. The average percent coefficient of variation (CV) for each counting method shown was calculated using cell concentration measurements at 50,000 cells/mL samples of 19 different cell lines. The Scepter cell counter is more precise than vision-based counting and hemocytometry, and approaches the precision of the Coulter Counter standard (red bars). Error bars represent standard deviation.
Accurate Counts Every TimeYou work too hard to lose data to erroneous cell counts. Be confident that your counts are right the first time.
Scepter counting gives you the accuracy of the Coulter Counter™ at a price you can afford.
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GAIN INSIGHT THROUGH POWERFUL COUNTING TECHNIqUES
• See your cultures with unprecedented detail
• Easy-to-read histograms and numerical statistics
• Data manipulation and storage on Scepter device or with software
• Track cell health as you monitor histograms from one preparation to the next
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ACCURACY THROUGH SENSOR TECHNOLOGY• Precisely counts consistent sample volumes
• Accurately counts every cell in your sample
• Cell discrimination at sub-micron and sub-picoliter resolution
INTRICACY SIMPLIFIED BY TRULY AUTOMATED DATA ACqUISITION• Pipette once for data in seconds
• Use on-site right at the hood
• Intuitive and easy to use with on screen instructions
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Scepter Technology: More Intuitive, More Informative, and More Precise
Cell Counter Format Counting Methods Comments
Hemocytometer Slide and microscope Manual, vision-based User bias and human error create irreproducible results
Brand I Benchtop Automated vision-based system Object recognition software-dependent, requires focusing and calibration, and can produce unreliable results
Scepter™ Cell Counter Handheld Impedance-based cell detection Counts every cell, every time in very precise volumes to ensure accurate results
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Mea
sure
d Cel
l Con
cent
rati
on (Cel
ls/m
L)
Theoretical Cell Concentration (Cells/mL)
Coulter counter
Scepter™ cytometer
Flow cytometer
Automated vision-based counterHemocytometer
Theoretical cell concentration (cells/mL)
Figure 2. COS7 cell counts using various systems. Data shown are average of four replicates. Error bars represent standard deviation. COS7 cells represent one out of 19 total cell lines tested. Cells were harvested, diluted in PBS, and counted using a Coulter Counter to determine the theoretical starting concentrations. The original cell concentration was divided by the fold dilution at each serial dilution step to determine theoretical concentrations (x axis).
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0 50000 100000 150000 200000 250000 300000 350000 400000 450000 500000
Mea
sure
d Cel
l Con
cent
rati
on (Cel
ls/m
L)
Theoretical Cell Concentration (Cells/mL)
Coulter counter
Scepter™ cytometer
Flow cytometer
Automated vision-based counterHemocytometer
Theoretical cell concentration (cells/mL)
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0 50000 100000 150000 200000 250000 300000 350000 400000 450000 500000
Mea
sure
d Cel
l Con
cent
rati
on (Cel
ls/m
L)
Theoretical Cell Concentration (Cells/mL)
Coulter counter
Scepter™ cytometer
Flow cytometer
Automated vision-based counterHemocytometer
Theoretical cell concentration (cells/mL)
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1.0E+05
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0 50000 100000 150000 200000 250000 300000 350000 400000 450000 500000
Mea
sure
d Cel
l Con
cent
rati
on (Cel
ls/m
L)
Theoretical Cell Concentration (Cells/mL)
Coulter counter
Scepter™ cytometer
Flow cytometer
Automated vision-based counterHemocytometer
Theoretical cell concentration (cells/mL)
There is no need to subjectively determine
cell counts using vision-based counting
methods. The Scepter cell counter detects
every cell, and displays the population as a
histogram of cell size distributions. From
the histogram, count all the cells or use
the gating function to count a chosen
subpopulation. By monitoring changes in
your histogram, you can gain insight into the
health and quality of your cell culture from
one experiment to the next.
volume pL
Coun
t
0
300
Test3
3.124e5 / mLJun 11 20:45:13
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1 Axis= Diameter or Volume
2 Concentration
3 Mean Cell Volume
4 Mean Cell Diameter
volume pL
Coun
t
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300
Test3
3.124e5 / mLJun 11 20:45:13
24.40.11
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7
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0 50000 100000 150000 200000 250000 300000 350000 400000 450000 500000
Scep
ter
Cel
l Cou
nt (Cel
ls/m
L)
Theoretical Cell Concentration (Cells/mL)
K562 r2=0.9982
SF9 r2=0.998
U266 r2=0.9996
Mesenchymal SC r2=0.9938HeLa r2=0.999
Theoretical cell concentration (cells/mL)
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0 50000 100000 150000 200000 250000 300000 350000 400000 450000 500000
Scep
ter
Cel
l Cou
nt (Cel
ls/m
L)
Theoretical Cell Concentration (Cells/mL)
K562 r2=0.9982
SF9 r2=0.998
U266 r2=0.9996
Mesenchymal SC r2=0.9938HeLa r2=0.999
Theoretical cell concentration (cells/mL)
0.0E+00
1.0E+05
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0 50000 100000 150000 200000 250000 300000 350000 400000 450000 500000
Scep
ter
Cel
l Cou
nt (Cel
ls/m
L)
Theoretical Cell Concentration (Cells/mL)
K562 r2=0.9982
SF9 r2=0.998
U266 r2=0.9996
Mesenchymal SC r2=0.9938HeLa r2=0.999
Theoretical cell concentration (cells/mL)
0.0E+00
1.0E+05
2.0E+05
3.0E+05
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5.0E+05
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0 50000 100000 150000 200000 250000 300000 350000 400000 450000 500000
Scep
ter
Cel
l Cou
nt (Cel
ls/m
L)
Theoretical Cell Concentration (Cells/mL)
K562 r2=0.9982
SF9 r2=0.998
U266 r2=0.9996
Mesenchymal SC r2=0.9938HeLa r2=0.999
Theoretical cell concentration (cells/mL)
Figure 3. Scepter counting performs with high linearity (R2 > 0.993) across multiple, diverse cell lines, over a wide operating range. Shown here are cell concentration data for five representative lines out of 19 total cell lines tested.
Exhaustively tested for your peace of mind We tested over 20 cell lines – including adherent, suspension, differentiated and progenitor cells –
to validate the Scepter cell counter’s precision and operating range.
Tested Cell Lines
Cell Line NameMeasured Average
Size (µm)Adherent / Suspension Origin Source
HEK293 13 - 15 adherent Human kidney Invitrogen Cat. No. R705-07
CHO-K1 15 adherent Chinese hamster ovary ATCC Cat. No. CCl-61
HeLa 13 adherent Human cervical cancer Millipore Cat. No. 20-385
NIH3T3 15 adherent Mouse fibroblasts ATCC Cat. No. CRL-1658,
MCF7 15 - 17 adherent Human breast adenocarcinoma
ATCC Cat. No. HTB-22
Cos-7 15 adherent Monkey kidney cells Genlantis Cat. No. C505100
Jurkat 10 suspension T lymphocytes ATCC Cat. No. TIB-152
SF9 13 mix adh / susp Insect ovary (baculovirus expression)
ATCC Cat. No. CRL 1711
PC12 12 - 13 loosely adherent Rat adrenal gland ATCC Cat. No. 1721
HepG2 15 adherent Hepatocytes ATCC Cat. No. HB8065
K562 15 suspension Human bone marrow ATCC Cat. No. CCL243
U266 12 suspension B lymphocytes ATCC Cat. No. TIB-196
RNSC 11 - 13 adherent Rat neuronal stem cells Millipore Cat. No. scr021
Epithelia 14 - 15 adherent Epithelia CELLnTEC Cat. No. HPEK p.05
HUVEC 12 - 14 adherent Human endothelial Millipore/ISCO Cat. No. SCCE001
Mouse ESC 13 adherent Mouse embryonic stem cells
Millipore Cat. No. SCR012
Mesenchymal SC 15 - 16 adherent Human bone marrow mesenchymal SC
Millipore Cat. No. SCR108
3T3-L1 15 - 16 adherent Human pre-adipocytes ATCC Cat. No. CL-173
hybridoma 13 - 14 suspension Hybridoma (Irs1 pS522.17.5.2)
Children's Hospital Boston Cat. No. 05-1921
Table 1. The cell lines tested represent adherent cells, suspension cells, differentiated, pluripotent, normal and transformed cells, and cover the range of cell types used in most cell research laboratories.
Millipore, Advancing Life Science Together, and EmbryoMax are registered trademarks of Millipore Corporation. Scepter and the M mark are trademarks of Millipore Corporation. Excel is a registered trademark of Microsoft Corporation. Vi-CELL and Coulter Counter are registered trademarks of Beckman Coulter, Inc. Z2 is a trademark of Beckman Coulter, Inc. Countess is a trademark of Life Technologies, Inc.Lit. No. PB1264EN00 Printed in U.S.A. 01/10 BS-GEN-09-02652 © 2010 Millipore Corporation, Billerica, MA 01821 U.S.A. All rights reserved.
www.millipore.com
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SCEPTER CELL COUNTER AND ACCESSORIES
Description qty/Pk Catalogue No.
Scepter Handheld Automated Cell Counter 1 PHCC00000
Includes: Scepter Cell Counter 1
Scepter Software CD 1
O-Rings 2
Scepter Test Beads 1 PHCCBEADS
Scepter USB Cable 1 PHCCCABLE
Scepter Tips, 60 µm 50 PHCC60050
500 PHCC60500
Universal Power Adapter 1 PHCCPOWER
Scepter O-Ring Kit, contains two O-rings and one filter cover 1 kit PHCC0CLIP
ORDERING INFORMATION
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