article · aureus: a1, b1 and c1; e. coli: a2, b2 and c2) and after (s. aureus: a1’, b1’ and...

Polymer Chemistry ARTICLE This journal is © The Royal Society of Chemistry 20xx J. Name ., 2013, 00 , 1-3 | 1 Please do not adjust margins Please do not adjust margins Supporting Information Bio-inspired peptide decorated dendrimer for a robust antibacterial coating on hydroxyapatite Yaping Gou, a,b,# Xiao Yang a,# Libang He, b,# Xinyuan Xu, a Yanpeng Liu, a Yuebo Liu, b Yuan Gao, b Qin Huang, a Kunneng Liang, b Chunmei Ding, a Jiyao Li, b, * Changsheng Zhao a and Jianshu Li a, * a College of Polymer Science and Engineering, State Key Laboratory of Polymer Materials Engineering, Sichuan University, Chengdu, 610065, China. Fax: 86-28-85405402; Tel:86-28-85466755; E-mail: [email protected] (JS. Li) ; b State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, 610041, China. E-mail: [email protected] (JY. Li); # These authors contributed equally to this work. Electronic Supplementary Material (ESI) for Polymer Chemistry. This journal is © The Royal Society of Chemistry 2017

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Polymer Chemistry

ARTICLE

This journal is © The Royal Society of Chemistry 20xx J. Name., 2013, 00, 1-3 | 1

Please do not adjust margins

Supporting Information

Bio-inspired peptide decorated dendrimer for a robust antibacterial coating on hydroxyapatite

Yaping Gou,a,b,# Xiao Yang a,# Libang He,b,# Xinyuan Xu,a Yanpeng Liu,a Yuebo Liu,b Yuan Gao,b Qin Huang, a Kunneng Liang, b Chunmei Ding,a Jiyao Li,b,* Changsheng Zhaoa and Jianshu Lia,*

a College of Polymer Science and Engineering, State Key Laboratory of Polymer Materials Engineering, Sichuan University, Chengdu, 610065, China. Fax: 86-28-85405402; Tel:86-28-85466755; E-mail: [email protected] (JS. Li) ;

b State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, 610041, China. E-mail: [email protected] (JY. Li);

# These authors contributed equally to this work.

Electronic Supplementary Material (ESI) for Polymer Chemistry.This journal is © The Royal Society of Chemistry 2017

Page 2: ARTICLE · aureus: a1, b1 and c1; E. coli: a2, b2 and c2) and after (S. aureus: a1’, b1’ and c1’; E. coli: a2’, b2’ and c2’) being incubated in PBS for 4 weeks. Live bacteria

ARTICLE Journal Name

2 | J. Name., 2012, 00, 1-3 This journal is © The Royal Society of Chemistry 20xx

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Figure S1. Schematic illustration of the synthesis of bioinspired SSP-PAMAM-NH2 dendrimer.

Page 3: ARTICLE · aureus: a1, b1 and c1; E. coli: a2, b2 and c2) and after (S. aureus: a1’, b1’ and c1’; E. coli: a2’, b2’ and c2’) being incubated in PBS for 4 weeks. Live bacteria

Journal Name ARTICLE

This journal is © The Royal Society of Chemistry 20xx J. Name., 2013, 00, 1-3 | 3

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Figure S2. 1H NMR of acryloyl-PAMAM-NH2 (a) and SSP-PAMAM-NH2 (b).

Page 4: ARTICLE · aureus: a1, b1 and c1; E. coli: a2, b2 and c2) and after (S. aureus: a1’, b1’ and c1’; E. coli: a2’, b2’ and c2’) being incubated in PBS for 4 weeks. Live bacteria

ARTICLE Journal Name

4 | J. Name., 2012, 00, 1-3 This journal is © The Royal Society of Chemistry 20xx

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Figure S3. DLS data of (a) PAMAM-NH2 and (b) SSP-PAMAM-NH2 in solution.

Page 5: ARTICLE · aureus: a1, b1 and c1; E. coli: a2, b2 and c2) and after (S. aureus: a1’, b1’ and c1’; E. coli: a2’, b2’ and c2’) being incubated in PBS for 4 weeks. Live bacteria

Journal Name ARTICLE

This journal is © The Royal Society of Chemistry 20xx J. Name., 2013, 00, 1-3 | 5

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Figure S4. CLSM of HA disks coated with dendrimers, and the sample after PBS washing.

Page 6: ARTICLE · aureus: a1, b1 and c1; E. coli: a2, b2 and c2) and after (S. aureus: a1’, b1’ and c1’; E. coli: a2’, b2’ and c2’) being incubated in PBS for 4 weeks. Live bacteria

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6 | J. Name., 2012, 00, 1-3 This journal is © The Royal Society of Chemistry 20xx

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Figure S5. QCM-D results of the dendrimer adsorption behaviours on the surface of HA-coated quartz chips. Representative Δf curves of (a) PAMAM-NH2 and (b) SSP-PAMAM-NH2 at different concentrations.

Page 7: ARTICLE · aureus: a1, b1 and c1; E. coli: a2, b2 and c2) and after (S. aureus: a1’, b1’ and c1’; E. coli: a2’, b2’ and c2’) being incubated in PBS for 4 weeks. Live bacteria

Journal Name ARTICLE

This journal is © The Royal Society of Chemistry 20xx J. Name., 2013, 00, 1-3 | 7

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Figure S6. SEM image of the bare HA disk.

Page 8: ARTICLE · aureus: a1, b1 and c1; E. coli: a2, b2 and c2) and after (S. aureus: a1’, b1’ and c1’; E. coli: a2’, b2’ and c2’) being incubated in PBS for 4 weeks. Live bacteria

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8 | J. Name., 2012, 00, 1-3 This journal is © The Royal Society of Chemistry 20xx

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Figure S7. SEM images of typical biofilms of S. sanguinis (a1, b1 and c1) and F. nucleatum (a2, b2 and c2) on the surface of HA without treatment (a group, control), coated with PAMAM-NH2 (b group) and SSP-PAMAM-NH2 (c group).

Page 9: ARTICLE · aureus: a1, b1 and c1; E. coli: a2, b2 and c2) and after (S. aureus: a1’, b1’ and c1’; E. coli: a2’, b2’ and c2’) being incubated in PBS for 4 weeks. Live bacteria

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Figure S8. Colony-forming unit counts of biofilms formed on bare HA disk (control), PAMAM-NH2 coated HA, and SSP-PAMAM-NH2 coated HA. Data are presented as mean ± standard deviation.

Page 10: ARTICLE · aureus: a1, b1 and c1; E. coli: a2, b2 and c2) and after (S. aureus: a1’, b1’ and c1’; E. coli: a2’, b2’ and c2’) being incubated in PBS for 4 weeks. Live bacteria

ARTICLE Journal Name

10 | J. Name., 2012, 00, 1-3 This journal is © The Royal Society of Chemistry 20xx

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Figure S9. Live/dead bacteria staining of typical biofilms of S. aureus and E. coli on the surface of HA without treatment/control (a group), coated with PAMAM-NH2 (b group) and SSP-PAMAM-NH2 (c group), before (S. aureus: a1, b1 and c1; E. coli: a2, b2 and c2) and after (S. aureus: a1’, b1’ and c1’; E. coli: a2’, b2’ and c2’) being incubated in PBS for 4 weeks. Live bacteria dye green, dead bacteria dye red, adjacent live and dead bacteria are presented as yellow when they are merged.

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