august 29-september 1, 2010 in baltimore, md, usa http ...itp2010.okstate.edu/update8302010.pdf ·...

17th International Symposium and Exhibit on Electro- and Liquid Phase-separation

Techniques August 29-September 1, 2010 in Baltimore, MD, USA

ITP 2010 website: http://fp.okstate.edu/2010

Updated Scientific Program as of 8-30-2010 Prof. Ziad El Rassi Editor-In-Chief, Electrophoresis and Chair of ITP 2010 (ITP for the first time held in the USA) Janet Cunningham, Symposium/Exhibit Manager, Barr Enterprises Don’t miss the most recognized international meeting showcasing the latest developments in liquid phase separations including capillary electroseparations science and technology being held for the first time in the USA. Dynamic program of cutting-edge presentations by world renowned scientists. Topical issues important to discovery, development and production. Showcasing CE, microfluidics, CE-MS, sequencing, omics, detection, etc. The conference is a must to attend and will offer information that will help identify solutions to practical problems in microfluidics, DNA sequencing and separation, multidimensional separation approaches and hyphenation to detection methods including mass spectrometry coupling (e.g., CE-, CEC- and LC-MS), as well as the various omics areas including proteomics, glycomics, genomics, metabolomics, foodomics, etc., and other applications such as biotechnology, (bio)pharmaceutical analysis and bioanalysis, enantioseparations, environmental, food, forensic and clinical.

The list of world renowned presenters is impressive The scientific program is extremely strong

Bring your questions and discuss solutions with the experts

Basic Topics Novel approaches in separation, detection and sample preparation using capillary, chip-based electroseparation techniques including CZE, CIEF, CITP, MEKC, MEEKC, FEE, CEC, stationary phases and coatings, nanotechnology, microfluidics, dielectrophoresis, CE-MS, CEC-MS, etc.

Applications Various omics including proteomics, glycomics, genomics, metabolomics, foodomics, etc., and other applications such as biotechnology, pharmaceutical analysis, bioanalysis, forensic, clinical, enantiomers, small ion analysis, biomedical, environmental, agriculture.

One-Day Training Courses

Course 1: Capillary Electrophoresis for Biomolecules Learn how to develop methods and implement CE for the separation of biomolecules

and take away a thorough understanding of electrophoretic processes.

Course 2: CE-MS: Principles and Applications Discover practical applications of CE-MS in the fields of drug analysis, protein and peptide analysis,

proteomics and metabolomics, food analysis, polymer sciences and many more.

Please share with your colleagues – http://fp.okstate.edu/2010

17th International Symposium and Exhibit on Electro- and Liquid Phase-separations

Techniques August 29-September 1, 2010 in Baltimore, MD, USA

ITP 2010 website: http://fp.okstate.edu/2010

Welcome Message from the ITP 2010 Chair Prof. Ziad El Rassi Editor-In-Chief, Electrophoresis and Chair of ITP 2010 (ITP for the first time held in the USA) The 17th International Symposium on Electro- and Liquid Phase-separation Techniques (ITP 2010) represents a turning point in the ITP Symposium Series. In fact, ITP 2010 is a new edition held for the first time in the USA and has assembled an impressive scientific program and exhibition. This 17th edition has widened in scope to include not only electro-separations in various formats (e.g., slab gels, capillaries, microchips), but also other areas of liquid phase separations. The program offers 120 lectures and more than 80 posters from world renowned scientists including 8 plenary lectures and 15 keynote lectures distributed over a variety of parallel sessions during the 3 full days of the meeting. The program features 3 sessions on microfluidics including its developments in Asia-Pacific and the West, 5 different sessions on proteomics, glycoproteomics, biomarkers and other omics, 3 sessions on DNA separation, sequencing and recent genetic analysis technologies and a large assortment of sessions on fundamentals, enantioseparations, MS, affinity electrophoresis, CEC and EKC, multidimensional separations, bioanalysis, sample preparation and concentration, immunoassays, nanotechnology, instrumentation and devices, detection, (bio)pharmaceuticals, and many more. ITP 2010 edition has been set up in the spirit of a large meeting with (i) 8 plenary lectures featuring MS in capillary separations, proteomics, ionic liquid phases in capillary separations, lab-on-a-chip, electromigration of single protein and DNA molecules in nanopores, lectin-based structural glycomics, micro and nanofluidics of single molecules and single cells, and mass linked immunoselective analysis, and (ii) 15 keynote lectures on expanding the proteome coverage of organelles and cells; CE of enantiomers; CE-based immunoassays; acoustophoresis for microfluidic cell handling and sample processing; microfluidic pinwheel effect in bioanalysis; genetic analysis by CE arrays; microfabricated chemical instrumentation; aerosol analysis by microchip electrophoresis; single drop microextraction-CE-MS; monoliths; biomarker discovery; high-throughput chromatography and proteomics technology for therapeutic proteins; in vivo chemical sensing of neurotransmitters using high-speed chip electrophoresis; unraveling cell to cell signaling in the brain by CE-MS; polymer-based microchips for nucleic acids and proteins; and much more. ITP 2010 edition features cozy exhibition and vendor workshops populated by world leaders in instrumentation and products for electro- and liquid-phase separation techniques and microfluidics. In addition, the program offers two full day courses on CE-MS and CE of biomolecules taught by experts in their fields. Furthermore, having ITP 2010 taking place in Baltimore provides the participants with the opportunity to have fun in the vibrant city of Baltimore and to enjoy visiting very close culturally rich cities such as Washington DC, Philadelphia, New York and many more. Warm wishes, Ziad El Rassi ITP 2010 Symposium Chair

I T P 2 0 1 0 S y m p o s i u m S c i e n t i f i c P r o g r a m P a g e | 1

ITP 2010 Updated Scientific Program as of 8-30-2010 Most important conference in the world focusing on cutting-edge areas of scientific research related to the impact of electro- and liquid phase-separation techniques and microfluidics on fundamental and practical issues in science and technology in multiple disciplines. See many of the world’s leading authorities on liquid phase separations—the list is impressive! What’s New? An outstanding program packed with plenary, keynote, invited and contributed talks, parallel sessions, poster sessions, courses, vendor workshops and exhibition. Continue to submit abstracts for poster presentation through the web site. Don’t miss this must attend event being held for the first time in the USA. Abstracts are in electronic format only and have been emailed to the pre-registrants.

8 plenary lectures featuring MS in Capillary Separations, Proteomics, Ionic Liquid Phases in Capillary Separations, Lab-on-a-Chip, Electromigration of Single Protein and DNA Molecules in Nanopores, Lectin-Based Structural Glycomics, Micro and Nanofluidics of Single Molecules and Single Cells, and Mass Linked Immunoselective Analysis.

15 keynote lectures featuring expanding the proteome coverage of organelles and cells; CE of enantiomers; CE-based immunoassays; acoustophoresis for microfluidic cell handling and sample processing; microfluidic pinwheel effect in bioanalysis; genetic analysis by CE arrays; microfabricated chemical instrumentation; aerosol analysis by microchip electrophoresis; single drop microextraction-CE-MS; monoliths; biomarker discovery; high throughput chromatography and proteomics technology for therapeutic proteins; in vivo chemical sensing of neurotransmitters using high-speed chip electrophoresis; unraveling cell to cell signaling in the brain by CEMS; polymer-based microchips for nucleic acids and proteins; and much more.

5 sessions on Proteomics, Biomarkers, Glycoproteomics, and Other Omics.

3 sessions on Microfluidics including its developments in Asia-Pacific and the West.

3 sessions on DNA Separation, Sequencing and Genetic Analysis Technologies.

Sessions on Fundamentals, Enantioseparations, CE-MS, Affinity Electrophoresis, CEC and EKC, Multidimensional Separations, Sample Preparation and Concentration, Nanotechnology, Instrumentation and Devices, Detection, (Bio) Pharmaceuticals, Bioanalysis, Applications and many more.

See the PROGRAM AT-A-GLANCE


We thank the following organizations for their generous support of the program

Travel Award Sponsorship Recipients

SPONSORED BY CASSS

Donna Blackney, Drexel University, Philadelphia, PA, USA Lidia Foteeva, Russian Academy of Sciences, Moscow, RUSSIA

Jana Svobodova, Charles University, Prague, CZECH REPUBLIC

SPONSORED BY HPLC INC.

Ye Ai, Old Dominion University, Norfolk, VA, USA Dulan Gunasekara, University of Kansas, Lawrence, KS, USA

Aleksandra Prokhorova, Lomonosov Moscow State University, Moscow, RUSSIA Andrus Seiman, Tallinn University of Technology, Tallin, ESTONIA

Andrea Stanova, Comenius University, Bratislava, SLOVAKIA

MALLINCKRODT/BAKER Thank you to Mallinckrodt/Baker for providing general sponsorship which has

led to greater student involvement in the meeting.

I T P 2 0 1 0 S y m p o s i u m S c i e n t i f i c P r o g r a m P a g e | 3 Sign up Now for Training Courses on Sunday, August 29, 2010 Courses are open to meeting and non-meeting participants. Pre-registration is required. See details under the link to “Courses.” To register, please complete and return the Meeting Registration Form located under the link to "Registration". The ITP Symposium reserves the right, without notice, to modify the course material or schedules, as well as to amend the roster of course presenters. Certificates of completion recognizing attendance will be awarded. COURSE 1: CAPILLARY ELECTROPHORESIS FOR BIOMOLECULES (9:00AM - 5:00PM) Learn how to develop methods and implement CE for the separation of biomolecules and take away a thorough understanding of electrophoretic processes. Discussion of factors that influence the electrophoretic migration of molecules in fluid solution, how to optimize resolution, buffers and buffer additives. Commercial instrumentation. CZE of biomolecules: how to develop a well controlled separation. Capillary isoelectric focusing: how to select instruments, capillaries and ampholytes. Capillary sieving electrophoresis: polymer networks, method development strategies, size separations for SDS proteins. Injection and detection: when to use and how to optimize both electrokinetic and hydrodynamic injection, why corrected peak areas are required with CE. Stacking and trace enrichment. Practical case studies will be presented on heparin, erythropoietin, monoclonal antibodies and carbohydrates. COURSE 2: CE-MS: PRINCIPLES AND APPLICATIONS (9:00AM - 4:00PM) Discover practical applications of CE-MS in the fields of drug analysis, protein and peptide analysis, proteomics and metabolomics, food analysis, polymer sciences and many more. Fundamental and comprehensive information will be provided on various aspects of this hyphenated technique. Discuss how to get started in CE-MS. Learn about CE-MS instrumentation (interfaces, ionization modes and MS-instruments). Explore correct choice of electrolytes and sheath-liquids for CE-MS. Fundamental knowledge on CE instrumentation and operation principles is recommended.


ITP 2010 Scientific Program

17th International Symposium on Electro- and Liquid Phase-separation Techniques

(International Symposium on Capillary Electroseparation Techniques)

Symposium Chairman Ziad El Rassi, Oklahoma State University, Stillwater, OK, USA

Sunday, August 29, 2010 – Plenary Lectures, Exhibition, Reception Course 1: CAPILLARY ELECTROPHORESIS FOR BIOMOLECULES Time: 9:00 am - 5:00 pm Location: Tuscan Room, 3rd Floor Instructor: Robert Weinberger, Ph.D., CE Technologies, Inc., Chappaqua, NY, USA Course 2: CE-MS: PRINCIPLES AND APPLICATIONS Time: 9:00 am - 4:00 pm Location: Composite Room, 3rd Floor Instructors: Prof. Gerhardus (Ad) de Jong, University Utrecht, Utrecht, THE NETHERLANDS Prof. Christian W. Klampfl, Johannes Kepler University, Linz, AUSTRIA

Prof. Alejandro Cifuentes, National Research Council (CSIC), Madrid, SPAIN 4:30-7:00 pm SYMPOSIUM REGISTRATION (2nd Floor) 4:30-8:00 pm EXHIBITION OPEN

OPENING CEREMONY LOCATION: CORINTHIAN ROOM (2ND FLOOR)

5:25 pm Remarks by the Chair of ITP 2010. Ziad El Rassi, Department of Chemistry, Oklahoma State University, Stillwater, OK, USA

SESSION 1. OPENING PLENARY LECTURES LOCATION: CORINTHIAN ROOM (2ND FLOOR) Session Chair: Jeff Chapman, Beckman Coulter Inc., Brea, CA, USA The aim of this opening session is to set the stage with two important themes of the ITP meeting: Mass spectrometry and proteomics by two world-renowned scientists.

5:40 pm L-101 The Essential Role of Mass Spectrometry in Capillary Separation Techniques.

Barry L. Karger, Barnett Institute, Northeastern University, Boston, MA 02115, USA [PLENARY LECTURE]

6:20 pm L-102 The Proteome Buccaneers: How to Unearth Your Treasure Chest via Combinatorial

Peptide Ligand Libraries. Pier Giorgio Righetti, E. Boschetti, Politecnico di Milano, Department of Chemistry Materials and Chemical Engineering "Giulio Natta", Via Mancinelli 7, Milano 20133, ITALY [PLENARY LECTURE]

7:00-8:00 pm WELCOME RECEPTION IN THE EXHIBIT HALL

Sponsored in part by Beckman Coulter


Monday, August 30, 2010 – Plenary Lectures 7:30 am SYMPOSIUM REGISTRATION OPEN (2nd Floor) 9:00am-6:00pm EXPOSITION OPEN

SESSION 2. PLENARY LECTURES LOCATION: CORINTHIAN ROOM (2ND FLOOR) Session Chair: Barry Karger, Northeastern University, Boston, MA, USA This plenary lecture session will provide the participants with new frontiers in microfluidics and lab on a chip, namely single biomolecule and single cell analysis as well as biomedical study and diagnosis

8:00 am L-103 Micro- and Nanofluidics for Single Biomolecule and Single Cell Analysis.

Yoshinobu Baba, Department of Applied Chemistry, School of Engineering, First Institute for Innovative Nanobiodevice Research, Nagoya University, Chikusaku, Furocho, Nagoya 464-8603, JAPAN [PLENARY LECTURE]

8:30 am L-104 Lab on a Chip: A Key Platform for Biomedical Study and Diagnosis. Bingcheng Lin, Jianhua Qin, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Liaoning 116023, CHINA [PLENARY LECTURE]

9:00 am BREAK in Exhibit Hall

I T P 2 0 1 0 S y m p o s i u m S c i e n t i f i c P r o g r a m P a g e | 6 Monday, August 30, 2010 – Morning Parallel Sessions

SESSION 3A. PROTEOMICS 1 LOCATION: CORINTHIAN ROOM (2ND FLOOR) Session Chairs: Jun Hirabayashi, Research Center for Medical Glycoscience, Umezono, Tsukuba, JAPAN, and Iulia Lazar, Virginia Polytechnic Institute and State University, Blacksburg, VA, USA

9:30 am L-105 Expanding the Proteome Coverage of Organelles and Cells: Applications in Cardiac

Disease. Melanie White, Rebekah Gundry, Vidya Venkatraman, Jennifer E Van Eyk, Department of Medicine, Johns Hopkins University, Baltimore, MD, USA [KEYNOTE LECTURE]

10:00 am L-106 Catching the Brain Membrane Protein Train - A Novel Strategy for Enrichment and

Analysis. Ganna Shevchenko, Marcus O. D. Sjödin, David Malmström, Magnus Wetterhall, Jonas Bergquist, Department of Physical and Analytical Chemistry, Analytical Chemistry, Uppsala University, Uppsala, SWEDEN

10:25 am L-107 Quantitative NanoProteomics for Protein Complex (QNaoPX) Approach using Diverse

Affinity Chemical Probes. Shu-Hui Chen, Department of Chemistry, National Cheng Kung University, No.1, Ta-Hsueh Road, Tainan 701, TAIWAN

10:50 am L-108 Capillary Isotachophoresis-based Tissue Proteome Platform. Xueping Fang1, Tong

Guo1, Kejia Zhao1, Brian M. Balgley2, Weijie Wang2, Cheng S. Lee1, 1Department of Chemistry and Biochemistry, University of Maryland, College Park, MD 20742, USA; 2Calibrant Biosystems, 910 Clopper Road, Suite 220N, Gaithersburg, MD 20878, USA

11:15 am Pause

SESSION 3B. ENANTIOSEPARATIONS LOCATION: DORIC ROOM (4TH FLOOR) Session Chairs: Gyula Vigh, Texas A & M University, College Station, TX, USA, and Pavel Kubáň, Institute of Analytical Chemistry, Brno, CZECH REPUBLIC

9:30 am L-109 Capillary Electrophoresis Applied to Enantiomer Separation and Estimation of Helix

Inversion Barrier of Cationic Helicene-like Species. Vaclav Kasicka, D. Koval, P. Sazelova, L. Severa, L. Adriaenssens, F. Teply, Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, v.v.i., Flemingovo 2, 16610 Praha 6, CZECH REPUBLIC [KEYNOTE LECTURE]

10:00 am L-110 Mechanistic Aspects of Enantioseparations in Capillary Electrophoresis. Bezhan Chankvetadze, Institute of Physical and Analytical Chemistry and Molecular Recognition and Separation Science Laboratory, School of Exact and Natural Sciences, Tbilisi State University, Chavchavadze Ave 3, 0179 Tbilisi, GEORGIA

10:25 am L-111 Capillary Electrophoresis-Mass Spectrometry: Innovative Methodologies for Sensitive

Analysis of Chiral Compounds. Shahab A. Shamsi, Jun He, Department of Chemistry, Georgia State University, Atlanta, GA 30303, USA

10:50 am L-112 The Use of Derivatized Cyclofructans as Novel Chiral Selectors in CE and HPLC.

Zachary S. Breitbach, Ping Sun, Daniel Armstrong, The University of Texas at Arlington, Chemistry and Biochemistry Department, Arlington, TX 76019, USA

11:15 am Pause


SESSION 3C. AFFINITY ELECTROPHORESIS (session organized by Terry Phillips) LOCATION: COMPOSITE ROOM (3RD FLOOR) Session Chair: Terry Phillips, NIH, Bethesda, MD, USA The aim of this special session is to revive and illustrate the importance and unsurpassed capability of CE and microfluidics in measuring and assaying bio-molecular interactions

9:30 am L-113 Capillary Electrophoresis-Based Immunoassays: An Overview of Principles and

Quantitative Applications. David S. Hage, Annette C. Moser, Dept. Chemistry, University of Nebraska-Lincoln, Lincoln, NE, USA, and University of Nebraska-Kearney, Kearney, NE, USA [KEYNOTE LECTURE]

10:00 am L-114 Combining Affinity CE and Taylor Dispersion Analysis (Nanosizing) for Efficient Protein

Characterization. Jesper Østergaard, Henrik Jensen, Department of Pharmaceutics and Analytical Chemistry, Faculty of Pharmaceutical Sciences, University of Copenhagen, Copenhagen, DENMARK

10:25 am L-115 Microfluidic Western Blotting. Amy E. Herr, University of California, Berkeley, CA, USA 10:50 am L-116 ACE with Proteins: Precise Characterization of Complex Binding Behaviour.

Deia El-Hadya, Sascha Kühneb, Adhitasari Suratmanb, Michael Grafb, Nagwa El-Maalia, Hermann Wätzigb, aAssiut University, Faculty of Science, Chemistry Department, Assiut-71516, EGYPT; bInstitute of Pharmaceutical Chemistry, TU Braunschweig, Braunschweig, GERMANY

11:15 am Pause

SESSION 4A. MULTIDIMENSIONAL APPROACHES IN MEMORIAM OF THE LATE PROF. DUSAN KANIANSKY LOCATION: CORINTHIAN ROOM (2ND FLOOR) Session Chairs: Pier Giorgio Righetti, Politecnico di Milano, Milano, ITALY, and Salvatore Fanali, Institute of Chemical Methodologies, Monterotondo Scalo, ITALY

11:20 am L-117 A Column-switching Tool by Joining CZE with ITP while Benefitting for Suppressed

Laminar and Electroosmosis Flows. Dušan Kaniansky, Róbert Bodor, Marián Masár, Department of Analytical Chemistry, Faculty of Natural Sciences, Comenius University, Mlynska Dolina CH-2, SK-842 15 Bratislava, SLOVAKIA

11:45 am L-118 Potential of Two-Dimensional Electro-Fluid-Dynamic Devices for Continuous Chemical

Purification. Chang Liu1, Yong Lou2, E. Jane Maxwell1, Ning Fang2, David D. Y. Chen1, 1Department of Chemistry, University of British Columbia, Vancouver, BC, V6T 1Z1, CANADA; 2Ames Laboratory-U.S. Department of Energy, Department of Chemistry, Iowa State University, Ames, IA 50011, USA

12:10 pm L-119 Improving Detection Sensitivity of ITP-CE in Medical Diagnostic Applications.

L. Bousse1, W. W. P. Chang1, Ring-Ling Chien1, C. Li1, T. Kawabata2, S. Satomura2, H.G. Wada1, 1Mountain View R&D Center, Wako Pure Chemical Industries, Ltd., Mt. View, CA, USA; 2New Diagnostics Business and Technology Development Department, Wako Pure Chemical Industries, Ltd., Osaka, JAPAN

12:30 pm Pause – Lunch on own


SESSION 4B. VARIOUS OMICS LOCATION: DORIC ROOM (4TH FLOOR) Session Chairs: Thomas Laurell, Lund University, Lund, SWEDEN, and James Landers, University of Virginia, Charlottesville, VA, USA

11:20 am L-120 Recent Applications of Capillary Electromigration Methods in Foodomics. Carolina

Simo, Virginia Garcia-Cañas, Clara Ibañez, Miguel Herrero, Alejandro Cifuentes, IFI, CSIC, Juan de la Cierva 3, 28006 Madrid, SPAIN

11:45 am L-121 Separation Capillary as a Microreactor: A Proof of Principle in In vitro Metallomic

Studies by CE. Andrei R. Timerbaev1, L. S. Foteeva1, J. K. Abramski2, K. Pawlak2, M. Jarosz2, 1Vernadsky Institute of Geochemistry and Analytical Chemistry, Moscow, RUSSIA; 2Chair of Analytical Chemistry, Warsaw University of Technology, POLAND

12:10 pm L-122 Site-Specific Quantification of Phosphorylated Cardiac Troponin I by MS-based Multiple

Reaction Monitoring. Pingbo Zhang1, Viola Kooij2, Anne M. Murphy1, Jolanda Van der Velden2, Cris dos Remedios3, Jennifer E. Van Eyk1, 1Johns Hopkins University School of Medicine, Baltimore, MD, USA; 2Institute for Cardiovascular Research, VU University Medical Center, Amsterdam, THE NETHERLANDS; 3Institute for Biomedical Research, The University of Sydney, Sydney, AUSTRALIA (Replaces the withdrawn lecture by Paolo Iadarola that appears in the printed program)


SESSION 4C. APPLICATIONS AND METHODOLOGIES 1 LOCATION: COMPOSITE ROOM (3RD FLOOR) Sessions Chairs: Reinhard H. H. Neubert, Martin Luther University, Halle (Saale), GERMANY and Michael T. Bowser, University of Minnesota, Department of Chemistry, Minneapolis, MN, USA

11:25 am L-123 A Microemulsion Electrokinetic Chromatography (MEEC) Method for Analysis of

Microcystins in Water. Grace Birungi, Sam F. Y. Li, Department of Chemistry, National University of Singapore, 3 Science Drive 3, Singapore 117543, SINGAPORE

11:45 am L-124 Capillary Electrophoretic Methods for Food Toxins Generated from Natural Food

Ingredients. F. Bedia Erim, Istanbul Technical University, Department of Chemistry, Maslak, Istanbul 34469, TURKEY

12:10 pm L-125 Application of Sheathless CE-MS for the Analysis of Posttranslationally Modified

Peptides. Herbert Lindner, Klaus Faserl, Leopold Kremser, Bettina Sarg, Division of Clinical Biochemistry, Biocenter, Innsbruck Medical University, Innsbruck, AUSTRIA


I T P 2 0 1 0 S y m p o s i u m S c i e n t i f i c P r o g r a m P a g e | 9 Monday, August 30, 2010 – Afternoon Parallel Sessions

FREE VENDOR WORKSHOP – Must register to attend free workshop If you are planning to attend the Free Vendor Workshop, you should sign up now to ensure your place in the workshop with a complimentary lunch. Seating that includes a lunch is limited. Contact: [email protected]

12:45-1:45 pm Agilent Electrophoresis Lunch & Learn Workshop

LOCATION: TUSCAN ROOM (3RD FLOOR) 1:50-2:55 pm POSTER SESSION in Exhibit Hall (5th Floor)

SESSION 5A. BIOMARKERS LOCATION: CORINTHIAN ROOM (2ND FLOOR) Session Chairs: Jennifer E Van Eyk, Johns Hopkins University, Baltimore, MD, USA, and Philip Britz-McKibbin, McMaster University, Hamilton, ON, CANADA

3:00 pm L-126 Disease Biomarker Discovery: Opportunities and Pitfalls. Haleem J. Issaq, Josip

Blonder, Timothy D. Veenstra, Laboratory of Proteomics and Analytical Technologies, Advanced Technology Program, SAIC-Frederick, Inc., NCI-Frederick, P.O. Box B, Frederick, MD 21702, USA

3:20 pm L-127 Assessment of a Modular, Multi-task Device Connected to Capillary Electrophoresis

and Other Chromatographic Instruments for the Enrichment, Separation and Identification of Protein Biomarkers. Norberto A. Guzman, Princeton Biochemicals Inc., Princeton, NJ 08543, USA

3:40 pm L-128 Microfluidic Chip-Capillary Electrophoresis for Emergent Clinical Diagnosis and

Monitoring of Biomarkers and Metabolites. Ying Sing Fung, Department of Chemistry, Hong Kong University, Pokfulam Road, Hong Kong SAR, CHINA

4:00 pm Pause

SESSION 5B. SAMPLE PREPARATION AND ENRICHMENT LOCATION: DORIC ROOM (4TH FLOOR) Session Chairs: Oliver Trapp, Ruprecht-Karls-Universität Heidelberg, Heidelberg, GERMANY, and David Ross, National Institute of Standards and Technology, Gaithersburg, MD, USA

3:00 pm L-129 Electric Field Enhanced Transport Across Phase Boundaries and Membranes for

Sample Pretreatment in Bioanalysis. Pavel Kubáň1, Lenka Langová1, Oanh Doan Thi Kieu2, Petr Gebauer1, Petr Kubáň2, Petr Boček1, 1Institute of Analytical Chemistry of the Academy of Sciences of the Czech Republic, v.v.i. Veveří 97, Brno, CZ-60200, CZECH REPUBLIC; 2Department of Chemistry and Biochemistry, Mendel University, Zemědělská 1, Brno, CZ-61300, CZECH REPUBLIC

3:20 pm L-130 Capillary Isoelectric Trapping of Proteins and Peptides: Submicroliter-volume

Fractionation, Desalting, Enrichment and Digestion. Ken K.-C. Yeung, University of Western Ontario, Department of Chemistry, London, ON N6A 5B7, CANADA

3:40 pm L-131 Transient Isotachophoretic Stacking in Zone Electrophoresis – Benefits and Risks. Petr

Gebauer, Zdenka Mala, Petr Bocek, Ludmila Krivankova, Institute of Analytical Chemistry of the Academy of Sciences of the Czech Republic, v. v. i., Veveri 97, 602 00 Brno, CZECH REPUBLIC

4:00 pm Pause


SESSION 5C. METHODOLOGIES LOCATION: COMPOSITE ROOM (3RD FLOOR) Sessions Chairs: W. Mike Arnold, MacDiarmid Institute at Industrial Research Ltd, Lower Hutt, NEW ZEALAND, and Koji Otsuka, Kyoto University, Kyoto, JAPAN

3:00 pm L-132 Capillary Zone Electrophoresis using Electrokinetic Supercharging Preconcentration –

From Small Ions to DNA Fragments. Takeshi Hirokawa, Zhongqi Xu, Kentaro Nakamura, Kouji Kawahito, Mihoro Yamada, Department of Applied Chemistry, Graduate School of Engineering, Hiroshima University, 1-4-1 Kagamiyama, Higashi-hiroshima, JAPAN

3:20 pm L-133 Chip-based Receptor Affinity CE for Measuring Bioactive Inflammatory Cytokines in

Human Biopsies. Heather Kalish, Terry Phillips, Edward Wellner, Ultramicro Immunodiagnostics Section, Laboratory of Bioengineering and Physical Sciences, National Institute of Biomedical Imaging and Bioengineering, Bethesda, MD 20892, USA

3:40 pm L-134 New Approaches in Pharmaceutical Analysis by LC-ESI-IT-MS. Zilin Chen, Qinhua

Chen, Stefanie Weißig, Shangwen Luo, Institute of Pharmaceutical Analysis, College of Pharmacy, Wuhan University, Wuhan 430072, CHINA

4:00 pm Pause

SESSION 6A. AFFINITY AND INTERACTIVE ELECTROPHORESIS LOCATION: CORINTHIAN ROOM (2ND FLOOR) Session Chairs: David S. Hage, University of Nebraska-Lincoln, Lincoln, NE, USA, and Prashanta Dutta, Washington State University, Pullman, WA, USA

4:05 pm L-135 Partial Filling Affinity Capillary Electrophoresis for Lipoprotein Studies.

Marja-Liisa Riekkola, Patricia Stege, Ai-Jun Wang, Lucia D’Ulivo, Joanna Witos , Geraldine Cilpa, Kati Vainikka, Katariina Öörnia, Petri T. Kovanena, Laboratory of Analytical Chemistry, Department of Chemistry, University of Helsinki, P.O. Box 55, FIN-00014 Helsinki, FINLAND; aWihuri Research Institute, Kalliolinnantie 4, FIN-00140, Helsinki, FINLAND

4:30 pm L-136 Genome-inspired DNA Aptamers for Nuclear Proteins. Linda B. McGown, Tian Zhang,

Brian Lin, Rensselaer Polytechnic Institute, Dept. of Chemistry and Chemical Biology, 321 Cogswell Laboratory, Troy, NY 12180, USA

4:55 pm L-137 Analysis of Human Biofluids using Chip-based Immunoaffinity Capillary Electrophoresis.

Terry M. Phillips, Heather Kalish, Edward Wellner, Ultramicro Immunodiagnostics Section, Laboratory of Bioengineering & Physical Science, National Institute of Biomedical Imaging and Bioengineering, NIH, Bethesda, MD, USA

5:20 pm L-138 Measuring Molecular Interactions using Micro Free Flow Electrophoresis.

Michael T. Bowser, Ryan T. Turgeon, Bryan R. Fonslow, Nicholas W. Frost, University of Minnesota, Department of Chemistry, 207 Pleasant St. SE, Minneapolis, MN, 55455, USA

5:45 pm Pause


SESSION 6B. NANOPARTICLE CAPILLARY ELECTROPHORESIS LOCATION: DORIC ROOM (4TH FLOOR) Session Chairs: Jozef Marák, Comenius University, Bratislava, SLOVAKIA, and Takashi Kaneta, Kyushu University, Fukuoka, JAPAN

4:05 pm L-139 Nanoparticle-based CEC for Protein Separation. Christian Nilsson1, Rickard Blom1, Ian

Harwigsson2, Staffan Birnbaum3, Staffan Nilsson1, 1Pure and Applied Biochemistry, Lund University, Lund, SWEDEN; 2Camurus AB, Lund, SWEDEN; 3Biopharma Support QC, Manufacturing Development, Novo Nordisk A/S, DENMARK

4:30 pm L-140 Characterization of Inorganic Nanoparticles by Capillary Electrophoresis and Capillary

Isotachophoresis. Ute Pyell1, W. Bücking2, T. Nann2,3, 1Department of Chemistry, University of Marburg, Hans-Meerwein-Strasse, D-35032 Marburg, GERMANY; 2Freiburg Materials Research Centre (FMF), Albert-Ludwig University of Freiburg, Stefan-Meier-Strasse 21, D-79104 Freiburg, GERMANY; 3School of Chemical Sciences and Pharmacy, University of East Anglia (UEA), Norwich NR4 7TJ, UK

4:55 pm L-141 New Facts in Separation of Microorganisms using Electromigration Techniques.

Michał Szumski1, Ewelina Dziubakiewicz1, Ewa Kłodzińska1, Katarzyna Hrynkiewicz2, Jacek Szeliga3, Marek Jackowski3, Bogusław Buszewski1, 1Chair of Environmental Chemistry and Bioanalytics, Faculty of Chemistry, 2Department of Microbiology, Faculty of Biology and Earth Science, 3Department of Surgery, Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University, Gagarina 7, Toruń, POLAND

5:20 pm L-142 Characterization of Quantum Dots Conjugates With Antibodies by Capillary

Electrophoresis. Ivona Voracova, Marcela Liskova, Karel Kleparnik, Vera Hezinova, Jan Prikryl, Frantisek Foret, Institute of Analytical Chemistry of the AS CR, v.v.i., Veveri 97, 602 00 Brno, CZECH REPUBLIC

5:45 pm Pause


SESSION 6C. INSTRUMENTATION AND DEVICES LOCATION: COMPOSITE ROOM (3RD FLOOR) Session Chairs: Cornelius F. Ivory, Washington State University, Pullman, WA, USA, and Gyoo Yeol Jung, POSTECH, Gyeongbuk, KOREA

4:05 pm L-143 Rapid Detection and Quantitation of Botulinum Neurotoxin/A Presence and Enzymatic

Activity using a Portable Diagnostic Platform. Chung-Yan Koh1, Gregory J. Sommer1, Bal Ram Singh2, Anup K. Singh1, Anson V. Hatch1, 1Sandia National Laboratories, Biosystems Research Department, Livermore, CA, 94551, USA; 2University of Massachusetts Dartmouth, Chemistry & Biochemistry Department, North Dartmouth, MA, 02747, USA

4:30 pm L-144 Capillary Electrophoresis (CE) as a Promising Technology for Field and Point-of-care

Instruments. Mihkel Kaljurand, Merike Vaher, Faculty of Science, Tallinn University of Technology, Akadeemia tee 15, Tallinn, ESTONIA

4:55 pm L-145 Lab-on-a-chip Device for Continuous Particle and Cell Separation Based on Electrical

Properties via AC-Dielectrophoresis. Barbaros Cetin1, Dongqing Li2, 1Mechanical Engineering, Middle East Technical University-Northern Cyprus Campus Guzelyurt, TRNC Mersin 10, TURKEY; 2Department of Mechanical & Mechatronics Engineering University of Waterloo, Waterloo N2L 3G1, Ontario, CANADA

5:20 pm L-146 Integration of Microchips, Capillary Electrophoresis, and Electrochemical Detection with

Robotic Platforms to Perform Remote Chemical Analysis. Claudimir L. do Lago1, Carlos A. Neves1, Eric Tavares da Costa1, Guilherme Minoru Otta1, Marcelo Fagundes Barros1, Thiago Garcia1, Arturo A. Ayon2, Carlos D. Garcia3, 1Institute of Chemistry, University of Sao Paulo, Sao Paulo, BRAZIL; 2MEMS Research Laboratory, The University of Texas at San Antonio, San Antonio, TX, USA; 3Department of Chemistry, The University of Texas at San Antonio, San Antonio, TX, USA

5:45 pm Pause Tuesday, August 31, 2010 – Plenary Lectures 7:30 am SYMPOSIUM REGISTRATION OPEN (2nd Floor) 9:00am-6:00pm EXPOSITION OPEN

SESSION 7. PLENARY LECTURES LOCATION: CORINTHIAN ROOM (2ND FLOOR) Session Chair: Fred Regnier, Perfinity Biosystems, West Lafayette, IN, USA This plenary lecture session will provide the participants with the backgrounds for a better understanding of the electromigration of biomacromolecules in nanopores as well as for the efficient utilization of lectins in glycomics

8:00 am L-201 Electromigration of Single Protein and DNA Molecules in Nanopores.

Edward S. Yeung, Changbei Ma, Ames Laboratory, U.S.D.O.E., Iowa State University, Ames, IA 50011, USA [PLENARY LECTURE]

8:30 am L-202 Lectin-based Structural Glycomics: Development of Lectin Microarray and Its Diverse

Applications. Jun Hirabayashi, Research Center for Medical Glycoscience, National Institute of Advanced Industrial Science and Technology, Central-2, 1-1-1, Umezono, Tsukuba 305-8568, JAPAN [PLENARY LECTURE]


I T P 2 0 1 0 S y m p o s i u m S c i e n t i f i c P r o g r a m P a g e | 13 Tuesday, August 31, 2010 – Morning Parallel Sessions

SESSION 8A. MICROFLUIDICS 1 LOCATION: CORINTHIAN ROOM (2ND FLOOR) Session Chairs: Tae Seok Seo, KAIST, Daejeon, KOREA, and Victor M. Ugaz, Texas A&M University, College Station, TX, USA

9:30 am L-203 Acoustophoresis - A Generic Platform for Microfluidic Cell Handling and Sample

Processing, Thomas Laurell, Dept. Measurement Technology and Ind. El. Eng.; Division of Nanobiotechnology, Lund University, Lund, SWEDEN [KEYNOTE LECTURE]

10:00 am L-204 The Microfluidic ‘Pinwheel Effect’: Label-Free DNA Detection, Quantitation, Cell

Counting and More. J. Li1, D. Leslie1, D. Finkler1, S. Barker2, James P. Landers1,3,4, 1Depts. of Chemistry, 2Electrical Engineering, and 3Mechanical Engineering, University of Virginia, 4Dept. of Pathology, University of Virginia Health Sciences Center, Charlottesville, VA 22904, USA [KEYNOTE LECTURE]

10:25 am L-205 Microfluidic Glass Chips with Integrated Nanoelectrospray Emitters for Chip

Electrophoresis – MS Coupling. Detlev Belder, University of Leipzig, Faculty of Chemistry and Mineralogy, Institute of Analytical Chemistry, Linnéstr. 3, 04103 Leipzig, GERMANY

10:50 am L-206 Microscale Isoelectric Fractionation Membranes for Rapid Protein Purification and

Enrichment. Greg J. Sommer, Junyu Mai, Anup K. Singh, Anson V. Hatch, Sandia National Laboratories, Livermore, CA, USA

11:15 am Pause

SESSION 8B. BIOANALYSIS 1 LOCATION: DORIC ROOM (4TH FLOOR) Session Chairs: Gerard Rozing, Agilent Technologies, Waldbronn, GERMANY, and Djuro Josic, Brown University, Providence, RI, USA

9:30 am L-207 Finding the Genes that Carry Risk for Common Disease: Population Requirements and

Strategy for use of Parallel Denaturing Capillary Electrophoresis Arrays. William G. Thilly1, S. Morgenthaler2, P. O. Ekstrom3, I. W.Hunter1, 1MIT, Cambridge, MA, USA; 2Ecole Polytechnique Federale, Lausanne, SWITZERLAND; 3Radium Hospital, Oslo, NORWAY [KEYNOTE LECTURE]

10:00 am L-208 Micro and Nanotechnology in Bioanalysis. Frantisek Foret, Institute of Analytical Chemistry of the ASCR, v.v.i., Brno, CZECH REPUBLIC

10:25 am L-209 Separating DNA and Protein Molecules in Gel-free Solutions using Bare

Nanocapillaries. Shaorong Liu, Xiayan Wang Joann J. Lu, Department of Chemistry and Biochemistry, The University of Oklahoma, Norman, OK 73019, USA

10:50 am L-210 Analytical Assays Based on Capillary Electrophoresis for Biologically Interesting

Molecules Such as Taurine, Peptides and Heparine. Reinhard H. H. Neubert, Institute of Pharmacy, Martin Luther University, Wolfgang-Langenbeckstr. 4, D-06120 Halle (Saale), GERMANY

11:15 am Pause


SESSION 8C. MINIATURIZATION IN THE WEST – MICROFLUIDICS 2 (session organized by Franta Foret) LOCATION: COMPOSITE ROOM (3RD FLOOR) Session Chair: Frantisek Foret, Institute of Analytical Chemistry of the ASCR, Brno, CZECH REPUBLIC

This special session on miniaturization brings together authoritative western speakers to discuss new frontiers in the area of microfluidics

9:30 am L-211 Microfabricated Chemical Instrumentation: Systems for Acquiring Chemical and

Biochemical Information. J. Michael Ramsey, University of North Carolina, Chapel Hill, NC 27517-2316, USA [KEYNOTE LECTURE]

10:00 am L-212 Improved Optical Detection Setups for Microfluidic Devices. Thomas G. Jensen, Pelle Ohlsson,Jörg P. Kutter, Technical University of Denmark, Dept. of Micro and Nanotechnology, DK-2800 Lyngby, DENMARK

10:25 am L-213 Novel Assay and Portable Instrument for Label-Free Toxin Detection. M. Bercovici1,

G.V. Kaigala1, S.S. Bahga1, C.J. Backhouse2, Juan G. Santiago1, 1Stanford University, Stanford, CA 94305, USA; 2University of Alberta, Edmonton, T6G 2V4, CANADA

10:50 am L-214 Dielectrophoretic Manipulation of Particles Employing Low Frequency Alternating

Electric Fields in an Array of Insulators. Javier L. Baylon-Cardiel1, Nadia M. Jesús-Pérez1, Ana V. Chávez-Santoscoy1, Sergio O. Martínez-Chapa1, Blanca H. Lapizco-Encinas2, 1Biomems Research Group, Tecnológico de Monterrey, 64849 Monterrey NL, MEXICO; 2Microscale Bioseparations Laboratory, CINVESTAV-Monterrey, 66600 Apodaca NL, MEXICO

11:15 am Pause

SESSION 9A. (BIO)PHARMACEUTICALS LOCATION: CORINTHIAN ROOM (2ND FLOOR) Session Chairs: Bezhan Chankvetadze, Tbilisi State University, Tbilisi, GEORGIA, and Shahab Shamsi, Georgia State University, Atlanta, GA, USA

11:20 am L-215 New Approaches for Profiling of (Bio)pharmaceuticals by CE-MS.

Gerhardus J. de Jong, Paul Hommerson, Rob Haselberg, Govert W. Somsen, Department of Biomedical Analysis, Utrecht University, P.O. Box 80082, 3508 TB Utrecht, THE NETHERLANDS

11:50 am L-216 Multiplexed Capillary Electrophoresis with UV Detection to Support High Throughput

Pharmaceutical Research. Jolanta Plewa, Xiaoyi Gong, Margaret Figus, Dorothy Levorse, Lili Zhou, Christopher Welch, Merck & Co., Inc., Rahway, NJ 07065, USA

12:15 pm L-217 A Multisite Study of Assay Performance for a Microfluidic CE-SDS Assay System.

Joseph Siemiatkoski, Biogen Idec, Cambridge, MA 02142, USA



SESSION 9B. DETECTION DEVICES AND APPROACHES LOCATION: DORIC ROOM (4TH FLOOR) Session Chairs: Ute Pyell, University of Marburg, Marburg, GERMANY, and Haleem Issaq, SAIC-Frederick, Inc., Frederick, MD, USA

11:20 am L-218 Aerosol Analysis by Microchip Electrophoresis. Scott D. Noblitt1, Susanne V. Hering3,

Jeffery L Collett, Jr.2, Charles S. Henry1, 1Departments of Chemistry and 2Atmospheric Science, Colorado State University, Fort Collins, CO, USA; 3Aerosol Dynamics, Inc., Berkeley, CA, USA [KEYNOTE LECTURE]

11:50 am L-219 Electrochemical Detection on Capillary Electrophoresis Microchips: Reliable Analytical

Platforms for Analyte Sensing in Real Domains. Alberto Escarpa, Department of Analytical Chemistry and Chemical Engineering, University of Alcalá, E-28871 Alcalá de Henares. Madrid, SPAIN

12:15 pm L-220 Combined Contactless Conductometric, Photometric, and Fluorimetric Single Point

Detector for Capillary Separation Methods. Markéta Ryvolová1,2, Mahbub Akhter3, Jan Preisler2, Pavel Krásenský2, František Foret4, Peter C. Hauser5, Pleun Maaskant3, Mirek Macka1,6, 1Irish Separation Science Cluster, National Centre for Sensor Research and School of Chemical Sciences, Dublin City University, Dublin 9, IRELAND; 2Department of Chemistry, Masaryk University, Brno, CZECH REPUBLIC; 3Tyndall National Institute, Cork, IRELAND; 4Institute of Analytical Chemistry, Academy of Science of the Czech Republic, Brno, CZECH REPUBLIC; 5Department of Chemistry, University of Basel, SWITZERLAND; 6Australian Centre for Research on Separation Science and School of Chemistry, University of Tasmania, Hobart, AUSTRALIA


SESSION 9C. YOUNG SCIENTISTS 1 (papers will be presented as both a poster and a lecture) LOCATION: COMPOSITE ROOM (3RD FLOOR) Session Chairs: Marja-Liisa Riekkola, University of Helsinki, Helsinki, FINLAND, and Linda McGown, Rensselaer Polytechnic Institute, Troy, NY, USA

11:20 am L-221 Microchip Free-flow Electrophoresis. Stefan Köhler, C. Weilbeer, D. Belder, University

of Leipzig, Faculty of Chemistry and Mineralogy, Institute of Analytical Chemistry, Linnéstr. 3, 04103 Leipzig, GERMANY

11:35 am L-222 Drop-based IEF for Protein Separation. Ana Egatz-Gomez1, Wolfgang Thormann2,

1Biomedical Engineering Department, Texas A&M University, College Station, TX, USA; 2Department of Clinical Pharmacology and Visceral Research, University of Bern, Bern, SWITZERLAND

11:50 am L-223 Regulation of DNA Translocation through a Nanopore by a Fluidic Field Effect

Transistor. Ye Ai, Shizhi Qian, Department of Aerospace Engineering, Old Dominion University, Norfolk, VA 23529, USA

12:05 pm L-224 Potential of Surface-Enhanced Raman Scattering Detector for Electrophoresis.

Jan Prikryl, Karel Klepárník, František Foret, Institute of Analytical Chemistry of the ASCR, v.v.i., Veveri 97, 602 00 Brno, CZECH REPUBLIC

12:20 pm L-225 A Method to Separate Dielectrophoresis and AC Electroosmosis. Jingyu Wang,

H. Daniel Ou-Yang, Lehigh University, 16 Memorial Drive E., Bethlehem, PA 18015, USA 12:35 pm Pause – Lunch on own

I T P 2 0 1 0 S y m p o s i u m S c i e n t i f i c P r o g r a m P a g e | 16 Tuesday, August 31, 2010 – Afternoon Parallel Sessions

FREE VENDOR WORKSHOP – Must register to attend free workshop If you are planning to attend the Free Vendor Workshop, you should sign up now to ensure your place in the workshop with a complimentary lunch. Seating that includes a lunch is limited. Contact: [email protected]

12:45-1:45 pm Developments in Capillary Electrophoresis at Beckman Coulter

LOCATION: TUSCAN ROOM (3RD FLOOR) 1:50-2:55 pm POSTER SESSION in Exhibit Hall (5th floor)

SESSION 10A. MINIATURIZATION IN ASIA PACIFIC – MICROFLUIDICS 3 (session organized by Bingcheng Lin) LOCATION: CORINTHIAN ROOM (2ND FLOOR) Session Chair: Bingcheng Lin, Dalian Institute of Chemical Physics, Liaoning, CHINA This special session on miniaturization will present authoritative speakers from Asia-Pacific to facilitate exchange of ideas with researchers in the west

3:00 pm L-226 High Performance Separation and Detection of Biogenic Compounds by Microchip

Electrophoresis Using On-line Sample Concentration Techniques. Takayuki Kawai, Kenji Sueyoshi, Fumihiko Kitagawa, Koji Otsuka, Department of Material Chemistry, Graduate School of Engineering, Kyoto University, Katsura, Nishikyo-ku, Kyoto 615-8510, JAPAN

3:25 pm L-227 Application of Optofluidic Sensors for Highly Sensitive Immunoassay. Jaebum Choo,

Department of Bionano Engineering, Hanyang University, KOREA 3:50 pm L-228 Integrated Microfluidic Systems for Fast Virus Detection. Gwo-Bin Lee, Department of

Engineering Science, National Cheng Kung University, Tainan, TAIWAN 4:10 pm L-229 Polyelectrolytic Gel Plugs on Chip and Their Applications. Taek Dong Chung,

Department of Chemistry, Seoul National University, Seoul 151-747, KOREA 4:30 pm L-230 Novel Microfabrication and Surface Modification Methods for Polymer Microfluidic

Chips. W. Mike Arnold, Andrea Bubendorfer, Ingrid Hoek, Tim Kemmitt, Roderick Stanley, Industrial Research Limited and the MacDiarmid Institute for Advanced Materials and Nanotechnology. Gracefield Research Centre, P.O. Box 31-310, Lower Hutt, NEW ZEALAND

4:50 pm Pause


SESSION 10B. CE-MS LOCATION: DORIC ROOM (4TH FLOOR) Session Chairs: William G. Thilly, MIT, Cambridge, MA, USA, and Edward Yeung, Iowa State University, Iowa, USA

3:00 pm L-231 Direct Coupling of Single Drop Microextraction with Capillary Electrophoresis-Mass

Spectrometry. Jihye Kim, Kihwan Choi, Doo Soo Chung, Department of Chemistry, Seoul National University, Seoul 151-747, KOREA [KEYNOTE LECTURE]

3:30 pm L-232 Two Interfaces for Efficient Coupling of CE and MS: Iontophoretic Fraction Collection

for CE-MALDI-MS and Sheathless Interfacing of CE and ESI-MS through a Porous Tip. Jean-Marc Busnel, Beckman Coulter, Discovery Solutions Business Center, 250 S. Kraemer Blvd., Brea, CA 92822-8000, USA

3:50 pm L-233 Coupling CE to MS: The Plug and Play Approach vs. Customized Interface Design.

Christian W. Klampfl, Institute of Analytical Chemistry, Johannes Kepler-University Linz, Altenbergerstr. 69, A-4040 Linz, AUSTRIA

4:10 pm L-234 The Role of MS Detection in Ligand Exchange Capillary Electrophoresis.

Vincenzo Cucinotta, A. M. Contino, S. Giuffrida, G. Maccarrone, M. Messina, University of Catania, Department of Chemical Sciences, viale A. Doria, 6 -95125 Catania, ITALY

4:30 pm L-235 Optimizing the Union between Electrophoretic Mobility and Electrospray Ionization.

Jeff Chapman, Beckman Coulter, Fullerton, CA, USA 4:50 pm Pause


SESSION 10C. APPLICATIONS AND METHODOLOGIES 2 LOCATION: COMPOSITE ROOM (3RD FLOOR) Session Chairs: Terry Phillips, NIH, Bethesda, MD, USA and Ziad El Rassi, Oklahoma State University, Stillwater, OK, USA

3:00 pm L-236 Electromigration Acid-Base Titration via Moving Neutralization Boundary

Electrophoresis. Qing Yang§,†, Cheng-Xi Cao§, Shan-Sheng Huang†, Liu-Yin Fan§, §Laboratory of Bioseparation and Analytical Biochemistry, School of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, CHINA; †School of Environment and Chemistry, Shanghai Normal University, Shanghai 200056, CHINA

3:25 pm L-237 Preparation of Monolithic Capillary Columns for Proteome Analysis. Hanfa Zou,

Mingliang Ye, Fangjun Wang, Minghuo Wu, Renan Wu, CAS Key Laboratory of Separation Science for Analytical Chemistry, National Chromatographic R&A Center Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 457 Zhongshan Road, Dalian 116023, CHINA

3:50 pm L-238 New Insights on Solubilization Loci in SDS Micelles from QSRR and Molecular

Dynamics Experiments. Carlos A. Fuzo1, Luís G. Dias1, João P.S. Farah2, Marina F.M. Tavares2, 1Department of Chemistry, FFCLRP, University of Sao Paulo, 14040-901 Ribeirao Preto, BRAZIL; 2Institute of Chemistry, University of Sao Paulo, P.O. Box 26077, 05513-970, Sao Paulo, BRAZIL

4:10 pm L-239 Hydroxyethylcellulose-graft-poly (2-(dimethylamino)ethyl methacrylate) as Physically

Adsorbed Coating for Protein Separation by CE. Fuhu Cao1, Zhaofeng Luo2, Shuhua Peng1, Dan Zhou1, Yanmei Wang1, 1CAS Key Laboratory of Soft Matter Chemistry, Department of Polymer Science and Engineering, University of Science and Technology of China, Hefei 230026, P. R. CHINA; 2School of Life Science, University of Science and Technology of China, Hefei 230027, P. R. CHINA

4:30 pm L-240 Analysis of Fibre Dyes by Capillary Electrophoresis – Quadrupole Time-of-Flight Mass

Spectrometry. Jason Lee1, Philip Doble1, Claude Roux1, Lucas Blanes1, Jane Hemmings2, 1Centre for Forensic Science, University of Technology, Sydney, NSW, AUSTRALIA; 2Forensic and Data Centre, Australian Federal Police, Canberra, ACT, AUSTRALIA

4:50 pm Pause


SESSION 11A. PROTEOMICS AND GLYCOPROTEOMICS LOCATION: CORINTHIAN ROOM (2ND FLOOR) Session Chairs: Jonas Bergquist, Uppsala University, Uppsala, SWEDEN and Ying Sing Fung, Hong Kong University, Hong Kong SAR, CHINA

4:55 pm L-241 Off- and On-Line Fast and Efficient Release of Glycoprotein N-Glycans Prior to MS and

LC-MSMS. Yehia Mechref, Yazen R. Jmeian, Department of Chemistry, Indiana University, Bloomington, IN 47405, USA

5:20 pm L-242 Proteomics Workflows and the Challenges to Separate and Identify Thousands of

Proteins. Aran Paulus, Bio-Rad Laboratories, 6000 James Watson Drive, Hercules, CA 94547, USA

5:45 pm L-243 Rapid Analysis of N-Glycans By Microchip-CE-LIF. M. Takahashi, R. Detloff, R. Singh,

Bahram Fathollahi, Caliper Life Sciences, Microfluidics R&D, 850 Marina Village Parkway, Alameda, CA 94501-1038, USA (presented by Joseph Siemiatkoski, Biogen Idec)

6:10 pm Pause 7:30 pm Conference Banquet Dinner

Ticket Required. Misplaced or lost tickets will not be replaced.

SESSION 11B. GENOTYPING AND DNA SEQUENCING AND SEPARATION LOCATION: DORIC ROOM (4TH FLOOR) Session Chairs: Kevin D. Dorfman, University of Minnesota, Minneapolis, MN, USA, and James W. Schneider, Carnegie Mellon University, Pittsburgh, PA, USA

4:55 pm L-244 Free-solution DNA Sequencing in Micro- and Nano-electrophoresis Systems Enabled

by Protein Polymer Drag-tags. Jennifer Coyne Albrecht, Annelise Barron, Stanford University, Department of Bioengineering and (by courtesy) Chemical Engineering, 318 Campus Drive, W300B James H. Clark Center, Stanford, CA 94304, USA

5:20 pm L-245 An Examination of PCR Inhibition in Forensic DNA Genotyping by Real Time PCR and

Capillary Electrophoresis. Bruce McCord, Robyn Thompson, Silvia Zoppis, Maribel Funes, Department of Chemistry, Florida International University, University Park, Miami, FL 33199, USA; Kerry Opel, Division of Science and Mathematics, Upper Iowa University, 605 Washington Street, P.O. Box 1857, Fayette, IA 52142, USA

5:45 pm L-246 A Novel Method for Analyzing Primer Heterodimers using Free-solution Capillary

Electrophoresis to Probe Dimerization Risk. Samantha M. Desmarais, Thomas K. Leitner, Steven M. Wolinsky, Annelise E. Barron, Department of Bioengineering, Stanford University, Stanford, CA, USA


Ticket Required. Misplaced or lost tickets will not be replaced.


SESSION 11C. CAPILLARY ELECTROCHROMATOGRAPHY AND ELECTROKINETIC CHROMATOGRAPHY LOCATION: COMPOSITE ROOM (3RD FLOOR) Session Chairs: Adam T. Woolley, Brigham Young University, Provo, UT, USA, and Joe Foley, Drexel University, Philadelphia, PA, USA

4:55 pm L-247 Novel Neutral Monolithic Columns for Capillary Electrochromatography of a Wide

Range of Species. Ziad El Rassi, S. Karenga, Department of Chemistry, Oklahoma State University, Stillwater, OK 74078, USA

5:20 pm L-248 Pressurized Planar Electrochromatography as a High Performance Separation Mode

and an Effective Tool for Two-dimensional Separations. Tadeusz H. Dzido, Adam Chomicki, Paweł Płocharz, Aneta Hałka, Piotr Ślązak, Anna Klimek-Turek, Department of Physical Chemistry, Medical University of Lublin, Staszica 6, 20081 Lublin, POLAND

5:45 pm L-249 Self-assembled Phospholipids: A Thermally Responsive Additive for Enhanced

Capillary Electrophoresis Separations. Lisa A. Holland, Stephanie Archer-Hartmann, Xingwei Wu, Ted Langan, C. Eugene Bennett Chemistry Department, West Virginia University, Morgantown, WV 26506, USA


Ticket Required. Misplaced or lost tickets will not be replaced. Wednesday, September 1, 2010 – Plenary Lecture 8:00 am SYMPOSIUM REGISTRATION OPEN (2nd Floor)

SESSION 12. PLENARY LECTURE LOCATION: CORINTHIAN ROOM (2ND FLOOR) Session Chair: Gerhardus J. de Jong, Utrecht University, Utrecht, THE NETHERLANDS This plenary lecture is a must to hear to discover the benefits of associating ionic liquids with mass spectrometry analysis

8:15 am L-301 Ionic Liquids in Separations and Mass Spectrometry, a New Frontier.

Daniel W. Armstrong,University of Texas at Arlington, Arlington, TX 76019, USA [PLENARY LECTURE]

8:45 am Pause

I T P 2 0 1 0 S y m p o s i u m S c i e n t i f i c P r o g r a m P a g e | 21 Wednesday, September 1, 2010 – Morning Parallel Sessions

SESSION 13A. FUNDAMENTALS AND METHODOLOGIES 1 LOCATION: CORINTHIAN ROOM (2ND FLOOR) Session Chairs: Vaclav Kasicka,, Institute of Organic Chemistry and Biochemistry, Praha, CZECH REPUBLIC, and Detlev Belder, University of Leipzig, Leipzig, GERMANY

8:50 am L-302 System Peaks in Electrophoresis: Why Do They Appear and How to Avoid Them?

Bohuslav Gas, Charles University in Prague, Faculty of Science, Albertov 2030, Prague 2, CZECH REPUBLIC

9:15 am L-303 Investigation of the Stereodynamics of Molecules and Catalyzed Reactions by CE.

Oliver Trapp, Organisch-Chemisches Institut, Ruprecht-Karls-Universität Heidelberg, Im Neuenheimer Feld 270, 69120 Heidelberg, GERMANY

9:40 am L-304 Gradient Elution Moving Boundary Electrophoresis: A Simple Technique for Analysis of

Complex Samples. David Ross*,†, Paul Flanigan, IV‡, Alyssa Henry§, Jason Kralj†, Catherine Lo†, Matthew Munson†, Eugenia Romantseva†,**, Jonathan Shackman‡, Elizabeth A. Strychalski†, †National Institute of Standards and Technology; ‡Department of Chemistry, Temple University; §Applied Research Associates, Inc. *Current affiliation: National Institute of Biomedical Imaging and Engineering, Gaithersburg, MD, USA

10:05 am L-305 Stability Studies of Cysteine Functionalized CdSe Nanoparticles via Capillary Electrophoresis. Jared S. Baker, David F. Watson, Luis A. Colón, Department of Chemistry, The State University of New York at Buffalo, Buffalo, NY, USA


SESSION 13B. BIOANALYSIS 2 LOCATION: DORIC ROOM (4TH FLOOR) Session Chairs: Yehia S. Mechref, Texas Tech University, Lubbock, TX, USA, and Alejandro Cifuentes, IFI, CSIC, Madrid, SPAIN

8:50 am L-306 Has Capillary Electrophoresis Matured into a Robust and Routine Bio-analytical

Measurement Method? Assessment and Examples. Gerard P. Rozing, Christian Wenz, Agilent Technologies, Waldbronn, GERMANY

9:15 am L-307 Recent Results in Forensic Analysis by Using Capillary Electrodriven Techniques.

Salvatore Fanali, Zeineb Aturki, Institute of Chemical Methodologies, Italian National Council of Research, Area della Ricerca di Roma I, Via Salaria Km 29,300 – 00015 Monterotondo Scalo, ITALY

9:40 am L-308 Analysis of Proteins by Capillary Sieving Electrophoresis with Postcolumn

Derivatization/Laser-Induced Fluorescence Detection. Takashi Kanetaa,b, Takehito Oguraa, Totaro Imasakaa,b, aDepartment of Applied Chemistry, Graduate School of Engineering, Kyushu University, Fukuoka, JAPAN; bDivision of Translational Research, Center for Future Chemistry, Kyushu University, Fukuoka, JAPAN

10:05 am L-309 Biomarker Quantitation through Affinity Extraction Coupled with Capillary

Electrophoresis in a Microfabricated Device. Adam T. Woolley, Weichun Yang, Pamela Nge, Ming Yu, Department of Chemistry and Biochemistry, Brigham Young University, Provo, UT, USA



SESSION 13C. GENETIC ANALYSIS TECHNOLOGIES (session organized by Annelise Barron) LOCATION: COMPOSITE ROOM (3RD FLOOR) Session Chairs: Bruce McCord, Florida International University, Miami, FL, USA and David Schwartz, University of Wisconsin, Madison, WI, USA This special session will discuss the technological advances that have broadened our understanding of genomes

8:50 am L-310 Capillary Electrophoretic Microdevice for High-Performance Short Tandem Repeat

Genotyping. Tae Seok Seo, Department of Chemical and Biomolecular Engineering (BK21 program) and Institute for the BioCentury, KAIST, Gwahangno, Yuseong-Gu, Daejeon 305-701, REPUBLIC OF KOREA

9:15 am L-311 Selecting Gel Pore Morphologies to Exploit Entropic Trapping for Enhanced Microchip

Electrophoresis Separations. Nan Shi, Victor M. Ugaz, Artie McFerrin, Department of Chemical Engineering, Texas A&M University, College Station, TX 77843, USA

9:40 am L-312 A Singular View of the Genome. David C. Schwartz, Laboratory for Molecular and

Computational Genomics, University of Wisconsin-Madison, 425 Henry Mall, Madison WI, USA 10:05 am L-313 Peak Broadening in Micellar DNA Electrophoresis: Effect of Diffusion, Polydispersity,

and Micelle Exchange Kinetics. Angela Holmen, Stephen Istivan, James W. Schneider; Carnegie Mellon University, Department of Chemical Engineering, 5000 Forbes Avenue, Pittsburgh, PA, 15213, USA (Replaces the withdrawn lecture by John Leamon that appears in the printed program)


SESSION 14A. SAMPLE PREPARATION AND CONCENTRATION LOCATION: CORINTHIAN ROOM (2ND FLOOR) Session Chairs: Ludmila Krivankova, Institute of Analytical Chemistry, Brno, CZECH REPUBLIC and Mihkel Kaljurand, Tallinn University of Technology, Tallinn, ESTONIA

10:50 am L-314 Development of an Amine-reactive, Hydrophilic Fluorophore having pH-independent

Fluorescence Properties and Mobilities in the 3 < pH < 10 Range. Gyula Vigh, Ming-Chien Li, Roy Estrada, Texas A&M University, College Station, TX, USA

11:15 am L-315 Off-line Coupled Mass Spectrometry with the Sample Pretreatment by the Column-

coupling Capillary Isotachophoresis. Jozef Marák, Andrea Stanová, S. Gajdoštínová, Dušan Kaniansky, Department of Analytical Chemistry, Faculty of Natural Sciences, Comenius University, Mlynská Dolina CH2, SK-842 15 Bratislava, SLOVAKIA

11:40 am L-316 Isotachophoresis on a Microchip with a 100x Reducing Union. Danny Bottenus,

Cornelius F. Ivory, Voiland School of Chemical Engineering and Bioengineering, Washington State University, Pullman, WA 99164, USA

12:05 pm L-317 Principles and Research Applications for Modular Approach to Designing Microscale

Systems from Proof-of-Concept to Product. Yolanda Fintschenko, LabSmith, Inc., 4659 Las Positas Road, Suite C, Livermore, CA 94551, USA



SESSION 14B. PROTEOMICS 2 / METABOLOMICS LOCATION: DORIC ROOM (4TH FLOOR) Session Chairs: Norberto A. Guzman, Princeton Biochemicals Inc., Princeton, NJ, USA, and Andrei R. Timerbaev, Vernadsky Institute of Geochemistry and Analytical Chemistry, Moscow, RUSSIA

10:50 am L-318 Biomarker Discovery: It’s Much More than a Mass Spec Problem.

Timothy D. Veenstra, Laboratory of Proteomics and Analytical Technologies, NCI-Frederick, Frederick, MD 21702, USA [KEYNOTE LECTURE]

11:20 am L-319 Proteomic Analysis of MCF7 Breast Cancer Cell Cycle. Milagros Perez, Iulia M. Lazar,

Virginia Bioinformatics Institute and Department of Biological Sciences, Virginia Polytechnic Institute and State University, Blacksburg, VA, USA

11:45 am L-320 Comprehensive Thiol Redox Status Determination using CE-MS for Metabolomics.

Lisa D'Agostino, Richard Lee, Philip Britz-McKibbin, Department of Chemistry and Chemical Biology, McMaster University, Hamilton, ON, L8S 4M1, CANADA

12:10 pm L-321 Les Maîtres de L’orge: How to Nail Down the Beer Proteome via Combinatorial Peptide

Ligand Libraries. Elisa Fasoli, Pier Giorgio Righetti, Politecnico di Milano, Department of Chemistry, Materials and Chemical Engineering "Giulio Natta", Via Mancinelli 7, Milano 20131, ITALY



SESSION 14C. YOUNG SCIENTISTS 2 (papers will be presented as both a poster and a short lecture) LOCATION: COMPOSITE ROOM (3RD FLOOR) Session Chairs: Alberto Escarpa, University of Alcalá, Alcalá de Henares. Madrid, SPAIN, and Lisa A. Holland, West Virginia University, Morgantown, WV, USA

10:50 am L-322 A Rapid Penta STR Screening Method by Microchip Capillary Electrophoresis.

Maurice J. Aboud1, Marcus Gassmann2, Bruce McCord1, 1Florida International University, Department of Chemistry and Biochemistry, University Park, Miami, FL 33199, USA; 2Agilent Technologies, Hewlett-Packard Strasse 8, 76337 Waldbronn, GERMANY

11:05 am L-323 Comparison of Single-chiral Selector and Multi-chiral Selector Enantioseparation

Systems. Jana Svobodová, Pavel Dubský, Eva Tesarová, Bohuslav Gaš, Department of Physical and Macromolecular Chemistry, Faculty of Science, Charles University in Prague, Hlavova 8, 128 43, Prague 2, CZECH REPUBLIC

11:20 am L-324 Enhancing the Coverage of the Urinary Metabolome using Sheathless CE-ToF-MS.

Rawi Ramautar, Andre Deelder, Oleg A. Mayboroda, Biomolecular Mass Spectrometry Unit, Department of Parasitology, Leiden University Medical Center, Leiden, THE NETHERLANDS

11:35 am L-325 Capillary Zone Electrophoresis-Based Methods for Determining Metal Ion and Chelating

Agent Speciation, and Their use in Environmental Chemistry. Nathan E. Boland, Alan T. Stone, Department of Geography and Environmental Engineering, The Johns Hopkins University, 313 Ames Hall, 3400 N. Charles Street, Baltimore, MD 21218, USA

11:50 am L-326 Isoelectric Trapping Pre-fractionation Coupled with LC-MS/MS for Quantitative

Proteomic Studies of Adipocyte Mitochondria. Stephanie M. Cologna1, Billy W. Newton2, William K. Russell1, Arul Jayaraman2, Gyula Vigh1, David H. Russell1, 1Departments of Chemistry and 2Chemical Engineering, Texas A&M University, College Station, TX 77842-3012, USA

12:05 pm L-327 Flow in a Planar Electro-Chromatograph. René Laskowski1, H.-J. Bart1, C. Hofmann2,

G. Menges2, B. Werner2, P. Löb2, V. Hessel2, H. Gruber-Wölfler3, P. Feenstra3, M.-G. Braunbruck3, J. Khinast3, 1TU Kaiserslautern, Thermische Verfahrenstechnik, 67653 Kaiserslautern, GERMANY; 2Institut für Mikrotechnik Mainz GmbH, Mainz, GERMANY; 3Institute for Process and Particle Engineering, Graz University of Technology, Graz, AUSTRIA

12:20 pm L-328 Nanoscale Electrokinetic Transport of DNA in Pressure-Driven Flow.

Denitsa Milanova†, Juan G. Santiago†, Annelise E. Barron‡, †Mechanical Engineering Department, Stanford University, Stanford, CA, USA; ‡Bioengineering Department, Stanford University, Stanford, CA 94304, USA



Wednesday, September 1, 2010 – Afternoon Parallel Sessions 1:50-2:55 pm POSTER SESSION in Exhibit Hall (5th Floor)

SESSION 15A. MULTIDISCIPLINARY KEYNOTE LECTURES LOCATION: CORINTHIAN ROOM (2ND FLOOR) Session Chairs: Jörg P. Kutter, Technical University of Denmark, Lyngby, DENMARK, and Christian W. Klampfl, Johannes Kepler-University Linz, Linz, AUSTRIA The aim of this multidisciplinary session is to provide the broadest view of what is happening currently in various interrelated areas of the life sciences in one single session that will feature experts in different interrelated fields.

3:00 pm L-329 Unraveling Cell to Cell Signaling in the Brain with Electrophoretic Separations and Mass

Spectrometry. Jonathan V. Sweedler, Department of Chemistry, University of Illinois, Urbana, IL, USA

3:25 pm L-330 Use of High-throughput Chromatography and Proteomics Technology for the

Optimization of Production and Quality Control of Therapeutic Proteins. Djuro Josic, COBRE Center for Cancer Research Development and Brown University, Providence, RI, USA

3:50 pm L-331 In vivo Chemical Sensing of Neurotransmitters using High-speed Chip Electrophoresis.

Robert T. Kennedy, University of Michigan, Department of Chemistry, 930 N. University, Ann Arbor, MI 48109-1055, USA

4:15 pm L-332 Polymer-based Microchips for the High Resolution Electrophoretic Separation of

Nucleic Acids and Proteins: Successes and Failures, Steven A. Soper, Center for BioModular Microsystems, Department of Chemistry, Department of Mechanical Engineering, Louisiana State University, Baton Rouge, LA 70803, USA

4:40 pm Pause

SESSION 15B. DNA SEPARATION AND SEQUENCING LOCATION: DORIC ROOM (4TH FLOOR) Session Chairs: David C. Schwartz, University of Wisconsin-Madison, Madison WI, USA, and Shaorong Liu, The University of Oklahoma, Norman, OK, USA

3:00 pm L-333 Micelle-ELFSE Methods for Rapid Electrophoretic Separations of DNA.

James W. Schneider, Stephen Istivan, Angela Holmen, Max Fahrenkopf, Department of Chemical Engineering, Carnegie Mellon University, Pittsburgh PA 15213-3890, USA

3:25 pm L-334 DNA Electrophoresis in Microfabricated Post Arrays. Kevin D. Dorfman, Jia Ou,

Jaeseol Cho, Daniel W. Olson, Samuel J. Carpenter, Department of Chemical Engineering and Materials Science, University of Minnesota, Minneapolis, MN, USA

3:50 pm L-335 Sensitive and Multiplex Genetic Analysis System using CE-based SSCP Combined with

MLPA. Gyoo Yeol Jung1,2, Gi Won Shin2, Hee Sung Hwang2, Boram Chung2, 1Department of Chemical Engineering and 2School of Interdisciplinary Bioscience and Bioengineering, POSTECH, Hyoja-dong, Nam-gu, Pohang, Gyeongbuk 790-784, KOREA

4:15 pm L-336 High-fidelity Fraction Collection of Single-strand DNA Fragments Differing in Size by

Only One Base on a Microchip. Kai Sun1, Zheyu Li1, Misato Sunayama2, Ryoko Araki2, Kosei Ueno1, Masumi Abe2, Hiroaki Misawa1, 1Research Institute for Electronic Science, Hokkaido University, Kita-21 Nishi-10, Kita-ku, Sapporo 001-0021, JAPAN; 2National Institute of Radiological Sciences, Anagawa 4-9-1, Inage-ku, Chiba 263-8555, JAPAN

4:40 pm Pause

I T P 2 0 1 0 S y m p o s i u m S c i e n t i f i c P r o g r a m P a g e | 26 Wednesday, September 1, 2010 – Afternoon Parallel Sessions

SESSION 15C. FUNDAMENTALS AND METHODOLOGIES 2 LOCATION: COMPOSITE ROOM (3RD FLOOR) Session Chairs: Bohuslav Gas, Charles University in Prague, Prague 2, CZECH REPUBLIC, and Jesper Østergaard, University of Copenhagen, Copenhagen, DENMARK

3:00 pm L-337 Elastic Matching Method for Determination of Quasi Steady State in pH Dependent ITP.

Jaesool Shim1, Migyung Cho2, Prashanta Dutta3, 1School of Mechanical Engineering, Yeungnam University, Gyeongsan 712-749, KOREA; 2Dept. of Media Engineering, Tongmyong University, Nam-gu, Busan 608-711, KOREA; 3School of Mechanical and Materials Engineering, Washington State University, Pullman, WA 99164-2920, USA

3:25 pm L-338 Resolution Equation for Electrokinetic Chromatography Based on Retention Factors

and Mobilities. Joe P. Foley, Drexel University, Department of Chemistry, 3141 Chestnut St., Philadelphia, PA 19104, USA

3:50 pm L-339 Microfluidic Technologies for Large-Scale Proteomic Investigations – A Perspective on

Benefits and Challenges. Jarod L.Kabulski1, Iulia M. Lazar1,2, 1Virginia Bioinformatics Institute and 2Department of Biological Sciences, Virginia Polytechnic Institute and State University, Blacksburg, VA, USA

4:15 pm L-340 Separation of Short DNA in Nanoslit Arrays. Elizabeth A. Strychalski1*, Henry W. Lau2,

Lynden A. Archer2, 1Department of Physics, Cornell University, Ithaca NY, USA; 2School of Chemical and Biomolecular Engineering, Cornell University, Ithaca NY, USA; *Current Affiliation: National Institute of Standards and Technology, Gaithersburg, MD, USA

4:40 pm Pause

SESSION 16. CLOSING PLENARY LECTURE LOCATION: CORINTHIAN ROOM (2ND FLOOR) Session Chair: Steven A. Soper, Louisiana State University, Baton Rouge, LA 70803, USA This closing plenary lecture discusses new frontiers in immunoselective analysis. Don’t miss it!!

4:55-5:25 pm L-341 Mass Linked Immunoselective Analysis. Fred Regnier, Kevin Meyer, Nicholas Herold,

Perfinity Biosystems, 3000 Kent Avenue, West Lafayette, IN 47906, USA [PLENARY LECTURE]

5:25 pm CLOSING REMARKS

LOCATION: CORINTHIAN ROOM (2ND FLOOR)

Closing Remarks by the Chair of ITP 2010. Ziad El Rassi, Department of Chemistry, Oklahoma State University, Stillwater, OK, USA

Invitation by the Chair of ITP 2011. Bezhan Chankvetadze, Institute of Physical and Analytical Chemistry and Molecular Recognition and Separation Science Laboratory, School of Exact and Natural Sciences, Tbilisi State University, Tbilisi, GEORGIA

5:50 pm Meeting Adjourn


Poster Session Instructions • Each poster board size is 4 feet wide (121cm) by 4 feet high (121cm). When preparing the poster, be sure

to subtract a 1-inch (2.5cm) border to leave room for the poster to properly fit on the poster board. • Be sure to bring the supplies you will need to mount your poster, i.e., push pins, tape, scissors, etc. Do not

use Velcro, and do not write on or mark the poster boards. • All poster presentations should be put up on Monday, August 30, between 7:30AM and 1:00PM. • Do not remove your poster until after the poster session ends on Wednesday afternoon. Remove between

3:00PM and 6:00PM (be sure to remove the reprint envelope where people leave business cards to request reprints of your abstract). Anything remaining after 6:00PM will be discarded).

• All posters will remain up for viewing all day Monday and Tuesday and Wednesday.

Monday, August 30, 2010 (1:50-2:55 pm) Each poster board size is 4 feet wide (121cm) by 4 feet high (121cm)

Be sure to bring the supplies you will need to mount your poster, i.e., push pins, tape, scissors, etc. Do not use Velcro, and do not write on or mark the poster boards

Poster Session 1. Sample Preparation, Concentration and Detection P-101 Sensitivity Enhancement for Capillary Zone Electrophoresis using a Novel Hybrid Sample

Injection Mode. N. Kaewchoay1, Keiichi Fukushi1, Y. Yakushiji1, T. Hirokawa2, 1Graduate School of Maritime Sciences, Kobe University, 5-1-1 Fukaeminami-machi, Higashinada-ku,Kobe, JAPAN; 2Department of Applied Chemistry, Graduate School of Engineering, Hiroshima University, 1-4-1 Kagamiyama, Higashi-hiroshima, JAPAN

P-102 Potential of Surface-enhanced Raman Scattering Detector for Capillary Electrophoresis. Jan Prikryl,

Karel Klepárník, František Foret, Institute of Analytical Chemistry of the ASCR, v.v.i., Veveri 97, 602 00 Brno, CZECH REPUBLIC

P-103 Combination of Preparative Capillary Isotachophoresis and Mass Spectrometry in Analysis of High

Molecular Weight Compounds Present in Complex Matrices. Andrea Stanová, Jozef Marák, Monika Kondeková, Dušan Kaniansky, Department of Analytical Chemistry, Faculty of Natural Sciences, Comenius University, Mlynská Dolina CH-2, SK-842 15 Bratislava, SLOVAK REPUBLIC

P-104 Numerical Computation and Experiments on Millions Fold Stacking of Peptides by a Derivative Moving

Reaction Boundary Initially Created by Formic Buffer and Strong Alkali in Electrophoresis. Jie Jina,§, Shan Lia,b,§, Wei Yana,§, Cheng-Xi Caoa, Wei Zhanga, Liu-Yin Fana, aLaboratory of Analytical Biochemistry & Bio-separation, Key Laboratory of Microbiology of Educational Ministry, School of Life Science & Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, CHINA; bSchool of Biological Science & Engineering, South China University of Technology, Guangzhou 510006, CHINA

P-105 PDA/LIF-CE as an Indispensible Analytical Tool in the Development of a Hydrophilic Amine-reactive

Fluorophor. Ming-Chien Li, Roy Estrada, Gyula Vigh, Texas A&M University, College Station, TX, USA

P-106 A Fully Automated Capillary Electrophoresis Equipment for the Column-Coupling Technology as Joining Zone Electrophoresis with Isotachophoresis. Miroslava Halašiová, Róbert Bodor, Dušan Kaniansky Department of Analytical Chemistry, Faculty of Natural Sciences, Comenius University, Mlynská dolina, 842 15 Bratislava, SLOVAKIA

P-107 Sensitivity Enhancement for the Analysis of Amino Acids and Peptides by In-line Single Drop

Microextraction. Yang-ki Park, Kihwan Choi, Jihye Kim, Doo Soo Chung, Department of Chemistry, Seoul National University, Seoul 151-747, KOREA

I T P 2 0 1 0 S y m p o s i u m S c i e n t i f i c P r o g r a m P a g e | 28 P-108 Selective Sweeping of Overloaded Zn(II) and Transient Isotachophoresis of Trace Metals in

Highly Saline Samples. Kihwan Choi, Jihye Kim, Doo Soo Chung, School of Chemistry, Seoul National University, Seoul 151-747, KOREA

P-109 Analysis of Fibre Dyes by Capillary Electrophoresis – Quadrupole Time-of-Flight Mass Spectrometry.

Jason Lee1, Philip Doble1, Claude Roux1, Lucas Blanes1, Jane Hemmings2, 1Centre for Forensic Science, University of Technology, Sydney, NSW, AUSTRALIA, 2Forensic and Data Centre, Australian Federal Police, Canberra, ACT, AUSTRALIA

P-110 Separation and Detection of Reactive Nitrogen Species from RAW 264.7 Cells using Microchip

Electrophoresis with Amperometric and Laser Induced Fluorescence Detection. Dulan B. Gunasekara1,2, Matthew K. Hulvey1, Derek T. Jensen1, Emilie R. Mainz1, Giuseppe Caruso1, Susan M. Lunte1, 2, 3, Christopher T. Culbertson4, 1Ralph N. Adams Institute for Bioanalytical Chemistry, 2Department of Chemistry, 3Department of Pharmaceutical Chemistry, University of Kansas, Lawrence, KS 66047, USA; 4Department of Chemistry, Kansas State University, Manhattan, KS 66506, USA

P-111 In-Capillary Nanoreactor Based on Transverse Diffusion of Laminar Flow Profiles for Cytochrome P450

2C9 Isoform Reaction with Diclofenac. Roman Řemínek, Aleš Mádr, Zdeněk Glatz, Department of Biochemistry, Faculty of Science, Masaryk University, Kamenice 5, 625 00 Brno, CZECH REPUBLIC

Monday, August 30, 2010 (1:50-2:55 pm) Each poster board size is 4 feet wide (121cm) by 4 feet high (121cm)


Poster Session 2. Food and Beverage P-201 Evaluation of the Free Radical Scavenging Capability of Wheat Extracts by Capillary Electrophoresis.

Kati Helmja, M. Vaher, M. Kaljurand, Department of Chemistry, Tallinn University of Technology, Akadeemia tee 15, Tallinn 12618, ESTONIA

P-202 Electrophoretic Analysis of Toxic Heavy Metals in Water Resources with Contactless Conductivity

Detection. Sam F. Y. Li, H. F. Lau, N. M. Quek, Department of Chemistry, National University of Singapore, Kent Ridge 119260, SINGAPORE

P-203 Column-coupling Electrophoresis Chip Technology Operating Sequentially in Determining both the

Inorganic Cations and Anions in Water. Marián Masár, Pavol Kruk, Milan Luc, Dušan Kaniansky, Department of Analytical Chemistry, Faculty of Natural Sciences, Comenius University, Mlynská Dolina CH-2, SK-84215 Bratislava, SLOVAKIA

P-204 Determination of GHB (Good Night Cinderella) by Chromatography and Capillary Electrophoresis:

Review of Methods of Analysis of GHB and Its Analogues. Elizabete Campos de Lima, Diogo Lima da Silva, Universidade Federal do ABC, Centro de Ciencias Naturais e Humas, R. Sta. Adelia, 166, Bl. B sala 1040/Lab.204, Santo André 09210-170, BRAZIL

P-205 Determination of Acrylamide in Food by Capillary Electrophoresis with Quantum Dot-mediated LIF

Detection. Qidan Chen, Ying Sing Fung, Department of Chemistry, the University of Hong Kong, Pokfulam Road, Hong Kong SAR, CHINA

P-206 Determination of Phytic Acid and Inositolphosphates in Barley. František Kvasnička, Jana Čopíková,

Rudolf Ševčík, Jana Krátká, Andrej Syntytsya and Michal Voldřich, Institute of Chemical Technology, Technická 3, Prague 6, CZECH REPUBLIC

P-207 Analysis of Free Amino Acids in Honey. Vaclav Stanek, Hana Weiserova, Petr Cesla, Jan Fischer,

Pavel Jandera, Department of Analytical Chemistry, Faculty of Chemical Technology, University of Pardubice, Studentska 573, CZ-53210 Pardubice, CZECH REPUBLIC

I T P 2 0 1 0 S y m p o s i u m S c i e n t i f i c P r o g r a m P a g e | 29 P-208 New Capillary Electrophoresis Method for Enrichment Study of Virgin Olive Oil with Antioxidants

Phenolic Acid Came from Natural Herbs. J. J. Berzas Nevado, V. Rodriguez Robledo, C. Sánchez-Carnerero Callado, Department of Analytical Chemistry and Food Technology, University of Castilla-La Mancha, Avenida Camilo José Cela 10, 13071, Ciudad Real, SPAIN

P-209 A Rapid and Sensitive Genetic Identification Method for Detecting Beer-Spoilage Bacteria and Wild

Yeast. Yong Wu, Manami Saha, Jean-Marc Busnel, BeeNa Lee, Lily Nan, Handy Yowanto, Jeff Chapman, Discovery Solutions Business Center, Beckman Coulter Inc., Brea, CA, USA

Tuesday, August 31, 2010 (1:50-2:55 pm) Each poster board size is 4 feet wide (121cm) by 4 feet high (121cm)


Poster Session 3. Bioanalysis P-301 Quantification of Branched-chain Amino Acids of Ascites by Capillary Electrophoresis with Light-emitted

Diode-induced Fluorescence Detection. Po-Ling Chang1, Tai-Chia Chiu2, Tsang-En Wang2, Kuang-Chun Hu2, Yu-Hsien Tsai3, Cho-Chun Hu3, Ming-Jong Bair2, Huan-Tsung Chang4,1Department of Chemistry, Tunghai University, Taichung, TAIWAN; 2Division of Gastroenterology, Department of Internal Medicine, Mackay Memorial Hospital, Taipei, TAIWAN; 3Department of Applied Science, National Taitung University, Taitung, TAIWAN; 4Department of Chemistry, National Taiwan University, Taipei, TAIWAN

P-302 Rapid Detection of Biogenic Amines from Human Intracutaneous Microdialysate Samples on Microchip Withdrawn Electrophoresis Platforms. E. Guihena, D. Brennanb, M. Ludwigc, Detlev Belderc, W. L. Hod, B.

Ramsayd, M. L. O’Connelle, W. T. O’Connora, aGraduate Entry Medical School and Materials and Surface Science Institute, Faculty of Education and Health Sciences, University of Limerick, IRELAND; bTyndall National Institute, Lee Maltings, University College Cork, IRELAND; cLeipzig University, Leipzig, GERMANY; dDermatology Unit, Mid Western Regional Hospital, Limerick, IRELAND; eDept. of Physics, University of Limerick, IRELAND

P-303 Comprehensive Study on the Bio-safety of Single-walled Carbon Nanotubes. Ruibin Li1, Ren’an Wu1,

Fanjun Wang1, Hongwei Liu1, Hanfa Zou1, Ling Yang2, 1National Chromatographic R&A Center, Key laboratory of Separation Sciences for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, CHINA; 2Laboratory of Pharmaceutical Resource Discovery, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, CHINA

P-304 MEKC Profiles of Human HDL Phospholipids. Chin-Pong Chong, Mine-Yine Liu, Department of

Chemistry, National Changhua University of Education, Changhua 50058, TAIWAN

P-305 Enhancing the Coverage of the Urinary Metabolome using Sheathless CE-ToF-MS. Rawi Ramautar, Andre Deelder, Oleg A. Mayboroda, Biomolecular Mass Spectrometry Unit, Department of Parasitology, Leiden University Medical Center, Leiden, THE NETHERLANDS

P-306 A New RP-RPLC Approach for Large-scale Phosphorylation Analysis of Human Liver. Chunxia Song,

Guanghui Han, Xinning Jiang, Zhiyuan Yu, Fangjun Wang, Rui Chen, Mingliang Ye, Hanfa Zou, National Chromatographic R&A Center, Dalian Institute of Chemical Physics, The Chinese Academy of Sciences, Dalian 116023, P. R. CHINA

P-307 The Monitoring of Glycerol in the Microdialyzate of Adipose Tissues by CE with Contactless

Conductivity Detection. Petr Tuma, Eva Samcová, Klára Málková, Institute of Biochemistry, Cell and Molecular Biology; Third Faculty of Medicine, Charles University, Ruská 87, 100 00 Prague 10, CZECH REPUBLIC

P-308 Optimization of Background Electrolyte Conditions for Determination Neutral Carbohydrates in Different

Natural Sources by Capillary Electrophoresis. Merike Vaher, Jana Kazarjan, Department of Chemistry, Tallinn University of Technology, Akadeemia tee 15, 12618 Tallinn, ESTONIA

I T P 2 0 1 0 S y m p o s i u m S c i e n t i f i c P r o g r a m P a g e | 30 P-309 A Rapid Penta STR Screening Method by Microchip Capillary Electrophoresis. Maurice J. Aboud1,

Marcus Gassmann2, Bruce McCord1, 1Florida International University, Department of Chemistry and Biochemistry, University Park, Miami, FL 33199, USA; 2Agilent Technologies, Hewlett-Packard Strasse 8, 76337 Waldbronn, GERMANY

P-310 Isoelectric Trapping Pre-fractionation Coupled with LC-MS/MS for Quantitative Proteomic Studies of

Adipocyte Mitochondria. Stephanie M. Cologna1, Billy W. Newton2, William K. Russell1, Arul Jayaraman2, Gyula Vigh1, David H. Russell1, 1Departments of Chemistry and 2Chemical Engineering, Texas A&M University, College Station, TX 77842-3012, USA

P-311 Reduction of Protein Concentration Range Differences Followed by Multicolumn Fractionation Prior to 2-

DE LC-MS/MS Profiling of Serum Proteins. Subhashini Selvaraju, Ziad El Rassi, Department of Chemistry, Oklahoma State University, Stillwater, OK 74078-3071, USA

P-312 Comparability Study of Beckman PA800 Plus vs. Agilent 7100 CE for Running Capillary Sodium

Dodecyl Sulfate Electrophoresis. Junge Zhang, Simei Shan, Chaomei Lin, Analytical Development – Malvern, Pharmaceutical Development and Manufacturing Sciences, Johnson and Johnson Pharmaceutical Research and Development, Malvern, PA, USA

P-313 Multiplex Gene Expression Analysis Identifies and Characterizes Induced Pluripotent Stem Cells

Derived from Human Somatic Cells. Kathryn Sciabica, Akitsu Hotta, Knut Woltjen, Handy Yowanto, Jeff Chapman, Beckman Coulter Inc., P.O. Box 8000, Brea, CA, USA

P-314 Quantitative Shot-Gun Proteomics for Revealing Gender Differences in Enzyme Contents for Rat Liver

Microsome. Huang-Jen Huang, Shu-Hui Chen, Department of Chemistry, National Cheng Kung University, Tanan, TAIWAN

P-315 Separation of Fucosylated and Non-fucosylated Carbohydrated Associated with Monoclonal Antibodies

using Capillary Electrophoresis. Sushma Rampal, Mark Lies, Hans Dewald, Beckman Coulter, Brea, CA, USA

P-316 A Novel Strategy for the Simultaneous Detection of Alternatively Spliced Transcripts: A Study of MEF2C

Exon Covariation. Kathryn Sciabica, Tod Gulick, Handy Yowanto, Jeff Chapman, Benne Parten, Beckman Coulter Inc., P.O. Box 8000, Brea, CA, USA

P-317 Application of Sheathless CE-MS for the Analysis of Phospho-Peptides. Bettina Sarg1, L. Kremser1, A.

Sobieszek2, H. Lindner1, 1Division of Clinical Biochemistry, Biocenter, Innsbruck Medical University, AUSTRIA; 2Institute for Biomedical Aging Research, Life Science Center, Austrian Academy of Sciences, AUSTRIA

P-318 Analytical Techniques for Investigating the Metabolism and Blood Brain Barrier Transport of the

Neuropeptide Dynorphin A 1-17. Giuseppe Caruso1,2, 3, Courtney D. Kuhnline1,3, Jane V. Aldrich4, Terry M. Philips5, and Susan M. Lunte1,3,6, 1The University of Kansas, Department of Pharmaceutical Chemistry, Lawrence, KS, USA; 2The University of Catania, Department of Chemical Science, section of Biochemistry and Molecular Biology, ITALY; 3The Ralph N. Adams Institute for Bioanalytical Chemistry, Lawrence, KS, USA; 4The University of Kansas, Department of Medicinal Chemistry, Lawrence, KS, USA; 5The National Institute of Health, NIBIB, USA; 6The University of Kansas, Department of Chemistry, Lawrence, KS, USA

P-319 Simple and Rapid CE-UV Method for the Assessment of Trail Pheromone Compounds of Leaf-cutting

Ants Venom Glands. Amanda Araujo Leitão1; Paulo Cesar Muniz de Lacerda Miranda1; Ana Valéria Colnaghi Simionato1,2, Institute of Chemistry – University of Campinas, Campinas – São Paulo – BRAZIL; 2National Institute of Science and Technology in Bioanalytics


Tuesday, August 31, 2010 (1:50-2:55 pm) Each poster board size is 4 feet wide (121cm) by 4 feet high (121cm)


Poster Session 4. Forensic, Pharmaceutical and Clinical Analysis P-401 Direct MEKC Analysis of Metallodrugs: May Micelle Focusing Be a Successful Approach? Lidia S.

Foteeva1, A.R. Timerbaev1, T. Hirokawa2, 1Vernadsky Institute of Geochemistry and Analytical Chemistry, Moscow, RUSSIA; 2Department of Applied Analytical Chemistry, Hiroshima University, Higashi-hiroshima, JAPAN

P-402 Rapid Monitoring of Arrays of Amino Acids in Clinical Samples Using Capillary Electrophoresis with

Contactless Conductivity Detection. Eva Samcová, Petr Tuma, Institute of Biochemistry, Cell and Molecular Biology; Third Faculty of Medicine, Charles University, Ruská 87, 100 00 Prague 10, CZECH REPUBLIC

P-403 Assessment of Chiral Purity of Acyclic Nucleoside Phosphonates-based Anti-AIDS Drugs by Capillary

Electrophoresis. Petra Sazelova, Veronika Solinova, Vaclav Kasicka, Antonin Holy, Institute of Organic Chemistry and Biochemistry ASCR, v.v.i., Flemingovo 2, 16610 Prague 6, CZECH REPUBLIC

P-404 Extraction of Lapachol from Tabebuia avellanedae with Supercritical Carbon Dioxide followed by

Capillary Electrophoresis with UV Detection. José Alberto Fracassi da Silva, Paulo de Tarso Vieira e Rosa, Fernanda Pereira Barbosa, Alexandre Zatkovskis Carvalho, Aline Ferreira Mora, Institute of Chemistry, State University of Campinas, Campinas, SP, BRAZIL

P-405 Determination of Sodium Alginate by Capillary Zone Electrophoresis with Capacitively Coupled

Contactless Conductivity Detection. José Alberto Fracassi da Silva, Alexandre Zatkovskis Carvalho, Letícia Souza Leite Vieira, Institute of Chemistry, State University of Campinas, Campinas, SP, BRAZIL

P-406 Analytical Instrument Qualification in Capillary Electrophoresis Using Infrared Thermometry. Claudia

Cianciulli, Hermann Wätzig, Institute of Pharmaceutical Chemistry, TU Braunschweig, Beethovenstr. 55, 38106 Braunschweig, GERMANY

P-407 Study of Degradation Processes and Characterization of Omeprazole and Its Main Degradation

Products by Capillary Electrophoresis using Diode-array and Electrospray Ionization-Mass Spectrometry Detectors. J. J. Berzas Nevado, G. Castañeda Peñalvo, R. M. Rodríguez Dorado, V. Rodriguez Robledo, Department of Analytical Chemistry and Food Technology, University of Castilla-La Mancha, Avenida Camilo José Cela 10, 13071, Ciudad Real, SPAIN

P-408 Analysis of Amphetamine Analogues using the Portable Bioanalyzer 2100 Lab-on-a-Chip. Aimee Lloyd,

Lucas Blanes, Alison Beavis, Philip Doble, Claude Roux, Centre for Forensic Science, University of Technology, Sydney (UTS), Broadway, NSW, AUSTRALIA


Wednesday, September 1, 2010 (1:50-2:55 pm) Each poster board size is 4 feet wide (121cm) by 4 feet high (121cm)


Poster Session 5. Capillary Electrochromatography (CEC) and Electrokinetic Chromatography (EKC)

P-501 The use of Novel Ionic liquid-in-water Microemulsion without the Addition of Organic Solvents in a

Capillary Electrophoretic System. Jun Cao, Haibin Qu, Yiyu Cheng, Pharmaceutical Informatics Institute, Zhejiang University, 388 Yuhangtang Road, Hangzhou 310058, CHINA

P-502 Flow in a Planar Electro-Chromatograph. René Laskowski1, H.-J. Bart1, C. Hofmann2, G. Menges2, B.

Werner2, P. Löb2, V. Hessel2, H. Gruber-Wölfler3, P. Feenstra3, M.-G. Braunbruck3, J. Khinast3, 1TU Kaiserslautern, Thermische Verfahrenstechnik, 67653 Kaiserslautern, GERMANY; 2Institut für Mikrotechnik Mainz GmbH, Mainz, GERMANY; 3Institute for Process and Particle Engineering, Graz University of Technology, Graz, AUSTRIA

P-503 Continuous Full Filling Capillary Electrochromatography - Evaluation of a New Separation Technique

using Nanoparticles and Electrospray Mass Spectrometry. David Malmströma, Jakob Axéna, Jonas Bergquista, Peter Vibergb, Peter Spégelb,c, aDepartment of Physical and Analytical Chemistry, Analytical Chemistry, Uppsala University, Box 599, SE-751 24 Uppsala, SWEDEN; bNanosep AB, Lund, SWEDEN; cMolecular Metabolism, Lund University Diabetes Centre, Lund University, Malmö University Hospital, Clinical Research Center, 91:11:061, SE-205 02 Malmö, SWEDEN

P-504 Study of Different Membrane Models by Capillary Electrochromatography. Kert Martma, Xochitl

Martinez Ramirez, Kaia-Liisa Habicht, Ruth Shimmo#, Institute of Mathematics and Natural Sciences, Tallinn University, Narva mnt 25, 10120 Tallinn, ESTONIA, # formerly Kuldvee

P-505 Enantioseparation of Anionic Compounds using Eremomycin as Chiral Selector by Different Variants of

Capillary Electrophoresis. Aleksandra F. Prokhorova, M.V. Lebedeva, E.N. Shapovalova, S.M. Staroverov*, O.A. Shpigun, Lomonosov Moscow State University, Chemistry Department, Moscow, RUSSIA; *JSC BioChemMack S&T, Moscow, RUSSIA

P-506 Comparison of Single-chiral Selector and Multi-chiral Selector Enantioseparation Systems. Jana

Svobodová, Pavel Dubský, Eva Tesarová, Bohuslav Gaš, Department of Physical and Macromolecular Chemistry, Faculty of Science, Charles University in Prague, Hlavova 8, 128 43, Prague 2, CZECH REPUBLIC

P-507 Extraction of Xenoestrogens from Water and Tissue using Molecularly Imprinted Polymers. Renata

Gadzała-Kopciuch, Michał Szumski, Bogusław Buszewski, Chair of Environmental Chemistry and Bioanalytics, Faculty of Chemistry, Nicolaus Copernicus University, 7 Gagarin St, 87-100 Toruń, POLAND

P-508 Hydroxylated, Neutral Octadecyl Acrylate Monolith With Fast Electroosmotic Flow and its Applications to

the Separation of Various Solutes Including Peptides and Proteins in the Absence of Electrostatic Interactions. Samuel Karenga, Ziad El Rassi, Oklahoma State University, Department of Chemistry, Stillwater, OK 74078, USA

P-509 Neutral Naphthyl Methacrylate-based Monolithic Columns for RP-CEC via Hydrophobic and π

Interactions. Samuel Karenga, Ziad El Rassi, Oklahoma State University, Department of Chemistry, Stillwater, OK 74078, USA

P-510 Mixed Ligand Monolithic Columns for Reversed-Phase Capillary Electrochromatography via

Hydrophobic and π-Interactions. Samuel Karenga, Ziad El Rassi, Oklahoma State University, Department of Chemistry, Stillwater, OK 74078, USA

I T P 2 0 1 0 S y m p o s i u m S c i e n t i f i c P r o g r a m P a g e | 33 P-511 Controlling Retention, Selectivity and Magnitude of EOF by Segmented Monolithic Columns Consisting

of Octadecyl and Naphthyl Monolithic Segments. Applications to RP-CEC of Both Neutral and Charged Solutes. Samuel Karenga, Ziad El Rassi, Oklahoma State University, Department of Chemistry, Stillwater, OK 74078, USA

P-512 Naphthyl Methacrylate-phenylene Diacrylate Based Monolith (NPM) for RP-CEC of Both Neutral and

Charged Solutes via Hydrophobic and π-interactions. Samuel Karenga, Ziad El Rassi, Oklahoma State University, Department of Chemistry, Stillwater, OK 74078, USA

P-513 Novel Organic Polymer Monolith with Polar Ligands for Normal Phase Capillary

Electrochromatography/Chromatography of Polar Species and for Immuno-capturing of Specific Serum Proteins. Dilani Gunasena, Ziad El Rassi, Department of Chemistry, Oklahoma State University, Stillwater, OK 74078-3071, USA

P-514 Methacrylate-based Monoliths at Varying Alkyl Chain Length for CEC of Proteins and Peptides–

Preparation, Chromatographic Characterization and Performance. Chanida Puangpila, Ziad El Rassi, Department of Chemistry, Oklahoma State University, Stillwater, OK 74078, USA

Wednesday, September 1, 2010 (1:50-2:55 pm) Each poster board size is 4 feet wide (121cm) by 4 feet high (121cm)


Poster Session 6. Fundamentals and Methodologies

P-601 Regulation of DNA Translocation through a Nanopore by a Fluidic Field Effect Transistor. Ye Ai, Shizhi Qian, Department of Aerospace Engineering, Old Dominion University, Norfolk, VA 23529, USA

P-602 Microchip Free-flow Electrophoresis. Stefan Köhler, C. Weilbeer, D. Belder, University of Leipzig,

Faculty of Chemistry and Mineralogy, Institute of Analytical Chemistry, Linnéstr. 3, 04103 Leipzig, GERMANY POSTER TO BE PRESENTED DURING THE POSTER SESSION ON MONDAY

P-603 Evaluation of the Precision of Dual-opposite-injection Capillary Electrophoresis. Donna M. Blackney,

Joe P. Foley, Department of Chemistry, 3141 Chestnut Street, Philadelphia, PA 19104, USA

P-604 Enhancing Resolutions of the Analytes in Capillary Zone Electrophoresis as Preceded by Capillary Isotachophoresis with Discrete Spacers. Róbert Bodor, Vladimíra Jánošová, Dušan Kaniansky, Department of Analytical Chemistry, Faculty of Natural Sciences, Comenius University, Mlynská Dolina, 842 15 Bratislava, Slovakia

P-605 Capillary Zone Electrophoresis-Based Methods for Determining Metal Ion and Chelating Agent

Speciation, and Their use in Environmental Chemistry. Nathan E. Boland, Alan T. Stone, Department of Geography and Environmental Engineering, The Johns Hopkins University, 313 Ames Hall, 3400 N. Charles Street, Baltimore, MD 21218, USA

P-606 Electropherogram Processing Algorithms for Automatic Baseline Correction, Signal Alignment with the

Aid of Thermal Marks and Peak Identification for Portable Capillary Electrophoresis Instruments. Andrus Seiman, Merike Vaher, Mihkel Kaljurand, Department of Chemistry, Tallinn University of Technology, Akadeemia tee 15, 12618 Tallinn, ESTONIA

P-607 Proper Capillary Electrophoresis/Frontal Analysis (CE/FA) Assay for the Determination of Binding

Constants. Karine Vuignier, Jean-Luc Veuthey, Pierre-Alain Carrupt, Sophie Martel, Julie Schappler, School of Pharmaceutical Sciences, University of Geneva, University of Lausanne, 30, Quai Ernest-Ansermet, CH-1211 Geneva 4, SWITZERLAND

I T P 2 0 1 0 S y m p o s i u m S c i e n t i f i c P r o g r a m P a g e | 34 P-608 A Method to Separate Dielectrophoresis and AC Electroosmosis. Jingyu Wang, H. Daniel Ou-Yang,

Lehigh University, 16 Memorial Drive E., Bethlehem PA 18015, USA

P-609 Drop-based IEF for Protein Separation. Ana Egatz-Gomez1, Wolfgang Thormann2, 1Biomedical Engineering Department, Texas A&M University, College Station, TX, USA; 2Department of Clinical Pharmacology and Visceral Research, University of Bern, Bern, SWITZERLAND

P-610 Nanoscale Electrokinetic Transport of DNA in Pressure-Driven Flow. Denitsa Milanova†, Juan G.

Santiago†, Annelise E. Barron‡, †Mechanical Engineering Department, Stanford University, Stanford, CA, USA; ‡Bioengineering Department, Stanford University, Stanford, CA 94304, USA

P-611 DNA-Modified Sieving Polymers for Metagenomic Profiling in Capillary Electrophoresis. Yingying Dong,

Linda B. McGown, Department of Chemistry and Chemical Biology, Rensselaer Polytechnic Institute, Troy, NY, USA

P-612 Theoretical Models of Separation Selectivity for Charged Compounds in Micellar Electrokinetic

Chromatography. Chanida Puangpila, Amorn Petsom, Thumnoon Nhujak, Department of Chemistry, Chulalongkorn University, Bangkok 10330, THAILAND

P-613 Multi-patterning of Biological Samples on Glass Substrate and Inside the Fused Microchannel by Using

Photochemical Reaction. Kihoon Jang, Yan Xu, Yo Tanaka, Takehiko Kitamori, The University of Tokyo, Tokyo, JAPAN

P-614 Development of Antibody Patterning Method in Extended-Nano Channels. Hisashi Shimizu, Kazuma

Mawatari, Takehiko Kitamori, School of Engineering, The University of Tokyo, Department of Applied Chemistry, School of Engineering, Tokyo,JAPAN

P-615 Development of Pressure-Driven Liquid Chromatography in Extended-Nanochannels; Automated

Injection and Normal Phase Mode. Ryo Ishibashi, Kazuma Mawatari, Takehiko Kitamori, Department of Applied Chemistry, School of Engineering, The University of Tokyo, Tokyo,JAPAN

P-616 Evaluation of Permanent Capillary Coatings for CIEF. Dušan Koval1 ,Hervé Cottet2, Václav Kašička1,

1Ústav Organické Chemie a Biochemie AV ČR, v.v.i., Flemingovo nám. 2., 166 10 Prague 6, CZECH REPUBLIC; 2Institut des Biomolécules Max Mousseron (UMR 5247 CNRS - Université de Montpellier 1 - Université de Montpellier 2), Place Eugène Bataillon CC 017, 34095 Montpellier Cedex 5, FRANCE

P-617 Stability Studies of Cysteine Functionalized CdSe Nanoparticles via Capillary Electrophoresis. Jared S.

Baker, David F. Watson, Luis A. Colón, Department of Chemistry, The State University of New York at Buffalo, Buffalo, NY, USA


List of Exhibitors AGILENT TECHNOLOGIES 2850 Centerville Road Wilmington, DE 19808, USA 800-227-9770 www.agilent.com/chem Agilent Technologies, a leading supplier of life science and chemical analysis instrumentation and services, provides solutions to pharmaceutical, biopharmaceutical, environmental, foods & flavors, and hydrocarbon industries, making possible the identification, quantification and analysis of atomic, molecular, physical and biological applications. Our comprehensive portfolio of instrumentation and solutions include Capillary Electrophoresis (CE), OffGel Electrophoresis (OGE) and CE-Mass Spec for the faster and more sensitive analysis of complex chemical and pharma samples. BECKMAN COULTER 250 S. Kraemer Blvd., Brea, CA 92821, USA 800-742-2345 www.CELeader.com Beckman Coulter provides solutions for the separation, identification, quantitation and characterization of many analytes, including ions, nucleic acids, basic drugs, enantiomers, proteins and carbohydrates. Our development initiative encompasses hardware, software and chemistry, with a long list of advancements including our NEW PA 800 plus Pharmaceutical Analysis System, sheathless CE-MS, and GeXP Genetic Analysis System for highly sensitive multiplex gene expression. CALIPER LIFE SCIENCES 68 Elm Street, Hopkinton, MA 01748, USA 508-435-9500 www.caliperls.com “LabChip GX and GXII Microfluidic Systems®. Caliper’s LabChip GX and GXII provide high throughput bioanalysis of nucleic acids and proteins. Caliper’s proprietary microfluidic sipper chips enable sampling directly from either 96- or 384-well microplates. For processes from research through product QC. Key protein applications: Titre, expression optimization, purity, glycan screening. GxP Security Software® provides support for validated environments. “ EDAQ INC. 2205 Executive Circle, Colorado Springs, CO 80906, USA 719-576-7000 www.edaq.com eDAQ supplies analysis systems for CE and ICE applications. Capacitively-Coupled Contactless Conductivity Detection (C4D) systems are available that act as a CE controller/data recorder, or a simple analog detector that integrates with existing CE, IC, HPLC, systems. The detectors work with 150µ, 365µ CE tubing as well as 1/32, 1/16, 1/8 inch OD tubing or self made chips with the micro-fluidic platform. Also, newly released is the Microchip Capillary Electrophoresis System (MCE) which includes data analysis, control, sequencer, and microchips with on chip C4D electrodes.


List of Exhibitors LABSMITH, INC. 4659 Las Positas, Suite C, Livermore, CA 94550, USA 925-292-5161 www.labsmith.com LabSmith, Inc. develops and builds laboratory tools that further the art of research. LabSmith products control the often exasperating aspects of experimentation, including timing, synchronization, high voltage and current sourcing, fluid routing and event capture. We craft rugged, innovative, and affordable solutions to everyday lab chores, so that you can focus on science. Scientists use LabSmith equipment for everything from on-chip electrochromatography, sample preparation to imaging. WILEY-BLACKWELL PUBLISHING Wiley VCH Verlag GmbH Bochstrasse 12 D-69469 Weinheim, GERMANY www.wiley.com Wiley-Blackwell is the world's leading society publisher, publishing more than 1400 scholarly peer-reviewed journals including a vast selection of cell and molecular biology publications and a prestigious books collection, written and edited by the best scholars and instructors. Visit our exhibit to browse our extensive range of books and journals and collect a sample copy of our flagship separations journal ELECTROPHORESIS.

We thank the following organizations for their generous support of the program

• Protein characterization

• Small molecule analysis

• Genetic analysis

• Capillary electrophoresis-mass spectrometry (CE-MS)

Taking Capillary Electrophoresis to New Heights

Don’t miss Tuesday’s lunch workshop at 12:45 p.m.

“Developments in Capillary Electrophoresis at Beckman Coulter” in which we will cover our latest advances.

Light lunch will be served. No RSVP required.

For Laboratory Use Only. Not for use in diagnostic procedures. © Copyright 2010 Beckman Coulter, Inc. Beckman Coulter and the stylized logo are registered trademarks of Beckman Coulter, Inc. CE-MS is currently in development. B2010-11492

Beckman Coulter is determined to enhance your CE experience by developing key technologies and providing you solutions to help optimize your workflow and efficiency. From the highly sensitive multiplex gene expression capabilities of the GeXP to the high-resolution applications of the NEW PA 800 plus Pharmaceutical Analysis System, Beckman Coulter unlocks the power of best-in-class performance. Select from existing panels for biomarker validation and pathogen detection, or request customized multiplexes for cancer research and toxicity studies. Choose validated methods for cIEF, CE-SDS or glycan analysis, or develop custom separations using the powerful 32 KaratTM software. As the leader in CE technology, we provide solutions for the separation, identification, quantitation and characterization of many analytes, including ions, nucleic acids, basic drugs, enantiomers, proteins and carbohydrates. Our development initiative encompasses hardware, software and chemistry, with a long list of advancements including our NEW PA 800 plus Pharmaceutical Analysis System, sheathless CE-MS, and GeXP Genetic Analysis System.

Toll free: 888 965 6086email: [email protected]: www.edaq.com

eDAQ Inc.2205 Executive CircleColorado SpringsCO 80906-4137

Contactless conductivity detection

High voltage sequencer

Micronit™ electrophoresis chips

Powerchrom data acquisition

High voltage sequencer for

microchips

Contacless conductivity detector

system for CE and microchips

Micronit chip contactless conductivity cell Conductivity

detection headstage for CE separations

If not your missing out on:

FREE monthly Industry leading print magazine providing news and peer reviewed technical articles

FREE twice monthly digital magazine – The Column delivered to a total readership of over 52,000 across Europe, Asia and the Middle East

FREE Weekly e-Alerts delivered directly to the inboxes of over 20,000 industry professionals each week

FREE access to global website containing original content, archived articles, podcasts, webcast and online directories

•

•

•

•

Are you one of the 26,000 readers who subscribes FREE*

to LCGC Europe magazine?

SUBSCRIBE NOW at:www.chromatographyonline.com

*Free to qualifying readers

Editor-in-Chief

Ziad El Rassi, Stillwater, OK, USA

Senior Deputy Editor

Petr Bocek, Brno, Czech Republic

Deputy Editors

František Foret, Brno, Czech Republic

Bohuslav Gaš, Prague, Czech Republic

Jong Hoon Hahn, Pohang, South Korea

Jörg P. Kutter, Lyngby, Denmark

Bingcheng Lin, Dalian, China

Takashi Manabe, Matsuyama, Japan

Terry M. Phillips, Bethesda, MD, USA

Andreas M. Rizzi, Wien, Austria

Nancy C. Stellwagen, Iowa City, IA, USA

Victor M. Ugaz, College Station, TX, USA

Founding Editor

Bertold J. Radola, Munich, Germany

ELECTROPHORESIS is the leading journal for new analytical and preparative electrophoretic methods and innovative applications. Continuously striving to provide readers and authors with the best platform for communicationFeatures include:

Fast Track for rapid publication of papers describing important/breakthrough research results

“Emphasis on …” sections featuring a series of review and research papers that belong to a specifi c topic

1540

6

ELECTROPHORESIS

www.electrophoresis-journal.com

To read a free sample copy or to set e-mail alert visit

Editor-in-Chief

Ziad El Rassi,

Senior Deputy Editor

Petr Bocek,

Deputy Editors

František Foret,

Bohuslav Gaš,

Jong Hoon Hahn,

Jörg P. Kutter,

Bingcheng Lin,

Takashi Manabe,

Terry M. Phillips,

Andreas M. Rizzi,

Nancy C. Stellwagen,

Victor M. Ugaz,

Founding Editor

Bertold J. Radola,

ELECTROPHORESIS is the leading journal for new

ELECTROPHORESISImpact Factor:

3.509 *

(*Thomson Reuters Citation Report 2008)

Special Issues in 2010 CE and CEC Reviews

Fundamentals

CE-MS

CEC and EKC

Bioanalysis

Food Analysis

Microcolumn Separation Methods for Metabolomics

CE and CEC Innovations

Instrumentation in Chip and CE

Miniaturization in Asia Pacifi c

Miniaturization 2010

Volume 31. 24 Issues in 2010ISSN Print: 0173-0835ISSN Online: 1522-2683

august 29-september 1, 2010 in baltimore, md, usa http ...itp2010.okstate.edu/update8302010.pdf ·...

Documents