AtDi19. At1g56280

1 206aa

28 206aa

32 206aa

206aa1

34 206aa

8

7

1

1

206aa71

#

PB1 domain-containing protein. At3g26510

1 196aa

196aa1

#

3

152aa

Heat shock protein 1. At4g14830

1 152aa

1

#

12

152aa

Serine rich protein. At5g21940

1 264aa

1

#

2

248aa531

254aa561

258aa591

264aa631

264aa1071

Zn finger protein. At3g48360

1 364aa#

364aa2231

364aa2251

364aa2802

364aa2822

297aa

AtToc33. At1g02280

1 297aa

1

#

3

264aa11

NAD-dependent protein. At3g23820

1 460aa#

460aa3481

460aa3501

460aa4451

Alpha 1,2-mannosidase. At3g21160

1 572aa#

572aa4321

572aa4402

Figure S3

Summary of redundant prey clones recovered using AtCPK4 as bait. Dark line indicates full-length open reading frame. Length is indicated in amino acids (aa). Open lines indicate recovered prey clones and coordinates of open reading frame. Number (#) to the right of each line indicates the number of times each prey clone was recovered during screens.

Auxin transport protein (Pin7). At1g23080

1 619aa#

601aa3971

591aa3971

SEC5a. At1g76850

1 1090aa#

606aa3321

602aa3321

170aa

Endo/exonuclease. At5g43210

1 170aa

1

#

2

Unknown protein. At5g55660

1 579aa#

579aa2422

242aa

Ribosomal protein L7. At2g01250

1 242aa

1

#

1

134aa11

242aa101

Unknown protein. At3g42800

1 341aa#

205aa11

299aa1081

315aa1081

Figure S3, continued

297aa

Cytochrome c oxidase assembly protein. At1g02410

1 287aa

51

300aa

Chloroplast thylakoid formation 1. At2g20890

1 300aa

126

150aa

Glutaredoxin-like protein. At1g03850

1 150aa

1

609aa

Amino acid acetyltransferase. At2g22910

1 609aa

529

509aa

Beta-ketoacyl-CoA synthetase. At2g26640

1 509aa

414

236aa

Unknown protein . At2g36420

1 439aa

6

Unknown protein . At3g11960

1 1379aa

1379aa1242

530aa

Calcium-binding EF hand family protein. At3g20290

1 545aa

265

691aa

ABC transporter. At3g21090

1 691aa

529

198aa

Heat shock protein 2. At3g22530

1 198aa

31

411-417aa

Fructose-bisphosphatase precursor. At3g54050

1 417aa

371aa

PB1 domain-containing protein 2. At4g32070

1 811aa

103

450-461aa

Unknown protein . At5g03040

1 461aa

ATP-dependent Clp protease. At5g50920

1 929aa

920-929aa

244aa

60S ribosomal protein L7. At3g13580

1 244aa

1

642aa

RNA polymerase beta subunit-1 (rpoC1)

1 680aa

343

Figure S4

Summary of ‘single-hit’ prey clones recovered using AtCPK4 as bait. Dark line indicates full-length open reading frame.Length is indicated in amino acids (aa). Open lines indicate recovered prey clones and coordinates of open reading frame.

Figure S5

Summary of redundant prey clones recovered using AtCPK11 as bait. Dark line indicates full-length open reading frame.Length is indicated in amino acids (aa). Open lines indicate recovered prey clones and coordinates of open reading frame. Number (#) to the right of each line indicates the number of times each prey clone was recovered during screens.

H2A.Z. At1g52740

1 134aa

18 134aa

21 134aa

134aa42

41 134aa

134aa

134aa

20

22

Actin depolymerizing factor 3. At5g59880

139aa1

1 118aa

PB1 domain-containing protein. At3g26510

196aa1

120aa1

196aa1

192aa1

Unknown protein. At5g52550

360aa1

32 236aa

339aa33

256aa33

297aa1

AtToc33. At1g02280

211aa1

240aa1

126aa1

105aa1

Acyl-carrier protein. At1g65290

126aa1

Chloroplast thylakoid formation 1. At2g20890

1 300aa

178 300aa

162 300aa

198aa1

Heat shock protein 2. At3g22530

184aa39

39 173aa

Heat Shock Protein 1. At4g14830

152aa1

467aa1

Geranygeranyl reductase. At1g74470

467aa361

294 378aa

236 467aa

330aa

330aa208

Ubiquitin C-terminal hydrolase. At1g65650

206aa1

Di19. At1g56280

206aa28

206aa

1128aa

1

200aa

1

364aa

364aa84

1

280 364aa

Zn Finger protein. At3g48360

284 364aa

1

#1

1

1

11

1

2

2

#

1

1

11

1#

1

1

2

#

2

1

1

#2

1

1

#

3152aa1

#

11

1

#

3

#

21

#

11

#

11

1

#

11

#

1

1

Figure S6

168aa1

92 168aa

Zn Finger homeobox family protein. At1g14687

619aa1

587aa397aa

Auxin transport protein (Pin7). At1g23080

344aa1

329aa1

Myb transcription factor. At1g25550

1

HAC13 protein. At1g32750

609aa1

609aa529

Amino Acid acetyltransferase. At2g22910

264aa1

91aa20

Unknown Protein. At4g20950

981aa1

981aa918

C-terminal domain phosphatase-like 1. At4g21670

401aa1

352aa80

Calmodulin-binding protein. At5g07240

96aa1

96aa49

Hypoxia-responsive protein. At5g27760

187aa1

50aa1

60S ribosomal protein L18. At5g27850

487aa1

420aa302

6-Phosphogluconate dehydrogenase. At5g41670

1919aa

1422aa1149

Summary of ‘single-hit’ prey clones recovered using AtCPK11 as bait. Dark line indicates full-length open reading frame.Length is indicated in amino acids (aa). Open lines indicate recovered prey clones and coordinates of open reading frame.