pAsian-Australasian Association of Animal Production Societies

{> Scope of AAAP: AAAP is established to devote for the efficient animal production in theAsian-Australasian region through national, regional, international cooperation and academicconferences.

{> Brief History of AAAP: AAAP was founded in 1980 with 8 charter members representing 8countries-those are Australia, Indonesia, Japan, Korea, Malaysia, New Zealand, Philippines andThailand. Then, the society representing Taiwan joined AAAP in 1982 followed by Bangladesh in1987, Papua New Guinea in 1990, India and Vietnam in 1992, Mongolia, Nepal and Pakistan in1994, Iran in 2002, Sri Lanka and China in 2006 , thereafter currently 19 members.

{> Major Activities of AAAP: Biennial AAAP Animal Science Congress, Publications of theAsian-Australasian Journal of Animal Sciences and proceedings of the AAAP congress andsymposia and Acknowledgement awards for the contribution of AAAP scientists.

<> Organization of AAAP:· President: Recommended by the national society hosting the next biennial AAAP AnimalScience Congress and approved by Council meeting and serve 2 years.· Two Vice Presidents: One represents the present host society and the other represents nexthost society of the very next AAAP Animal Science Congress.· Secretary General: All managerial works for AAAP with 6 years term by approval by thecouncil· Council Members: AAAP president, vice presidents, secretary general and each presidentsor representative of each member society are members of the council. The council decidescongress venue and many important agenda of AAAP

<> Office of AAAP: Decided by the council to have the permanent office of AAAP in Korea.Currently # 909 Korea Sci &Tech Center Seoul] 35-703, Korea{> Official Journal of AAAP: Asian-Australasian Journal of Animal Sciences (AsianAustralas.1. Anim. Sci. ISSN 1011-2367. http://www.ajas.info) is published monthly{> Current 19 Member Societies of AAAP:ASAP(Australia), BAHA(Bangladesh), CAAV(China), IAAP(India), ISAS(Indonesia),lAAS(Iran), JSAS(Japan), KSAST(Korea), MSAP(Malaysia), MLSBA(Mongolia),NASA(Nepal), NZSAP(New Zealand), PAHA(Pakistan), PNGSA(Papua New Guinea),PSAS(Philippines), SLAAP(Sri Lanka), CSAS(Taiwan), AHAT(Thailand), AHAV(Vietnam).~ Previous Venues of AAAP Animal Science Congress and AAAP Presidents

I 1980 Malaysia S. Jalaludin II 1982 Philippines V. G. ArganosaIII 1985 Korea In Kyu Han IV 1987 New Zealand A. R. SykesV 1990 Taiwan T. P. Yeh VI 1992 Thailand C. ChantalakhanaVII 1994 Indonesia E. Soetirto VIII 1996 Japan T. MorichiIX 2000 Australia 1. Ternouth X 2002 India P. N. BhatXI 2004 Malaysia Z. A. Jelan XII 2006 Korea 1.K. PaikXIII 2008 Vietnam N.V. Thien XIV 2010 Taiwan i.c. HsiaXV 2012 Thailand CKittayachawengXVI 2014 Indonesia Yudi GuntaraXVII 2016 Japan SeiichiKoiznmi XVIII 2018 Malaysia Loh Teck Chwen

,* AAAP is the equal opportunity organization.•. Copyright®: AAAP

Welcome Message

The 17th Animal Science Congress of AAAPwill be held at Kyushu Sangyo University,

Fukuoka, Kyusyu Area in Japan, from 22 to 25 August 2016. The aim of this congress

is to provide a forum for the exchange of new information on animal sciences and

technology, with a focus on successful strategies for the sustainable promotion of

livestock considering the environment and welfare of livestock and human beings.

At the same time, the congress will provide a venue for people from both inside and

outside of the Asian Australasian region to make new contacts and renew friendships.

Japanese Society of Animal Science is organizing the 17th AAAP Congress and is

pleased to welcome everyone in this congress who is interested in animal science and

production.

The venue of the congress, Fukuoka City, where tradition meets modernity, with

delicious dishes and an excellent geographic location close to the Asian countries.

Prof. Mitsuhiro FURUSEPresident of 17th AAAP

r y y , '-.lW The 17th Asian-Australasian Association ofAnimal Production Societies Animal Science CongressAnimalScience

Congress

Committee Members

Mitsuhiro FURUSE

Seiichi KOIZUMI

KeiHANZAWA

Naomi KASHIWAZAKI

Masahiro SATOH

Koichi ANDO

Keitaro YAMANOUCHI

President

Chair of Committee and Finance

Chair of Fund Raising, Public Relations, and Registration

Chair of Accommodations & Tour

Chair of Program, Scientific Section and Publications

Chair of Venue, Social Culture & Protocol, and Exhibition

Secretary General

Naoshige ABEYoshikazu ADACHIRyozo AKUZAWANarito ASANUMAHisashi ASOTakashi BUNGOHiroshi noiuOsamu DOlTakafumi GOTOHTsutomu HASHIZUMESatoshi HIDAKAKohzy HIRAMATSUHiroyuki HIROOKAToshiyoshi ICHINOHEMasakazu IRIEYasuhiro KAWAMOTOTomoyuki KAWASHIMAKazuhiro KIKUCHIShinichi KOBAYASHIYasuo KOBAYASHITetsuo KUNIEDAHiroki MATSUITakashi MIYANOTetsuo MORITA

2

Takashi NAGAIKunihiko NAITOYoshitaka NAKANISHISueo NIIMURATakahiro NIKKIShotaro NISHIMURATakeyuki OZAWAHiroshi SASADAEimei SATOKazuhiro SHIMADAShigeru SHIOYAKunio SUGAHARAKoji SUGIURAMadoka SUTOHKenichi TAKEDAKumiko TAKEDARyuichi TATSUMIYoshinori TERAWAKIAtsushi TOYODAHiroko TSUKAMURAHitoshi USHUIMAAkira WATANABENobuhiko YAMAUCHITatsuyuki YOSHIDA

alphabetical order / titles omitted

~The 17th Asian-Australasian Association of Animal Production Societies Animal Science Congress ~--------------------------------------- Animal

C:':g~:s

Outline of the congress ,

Congress Name

The 17th Asian-Australasian Association of Animal Production Societies Animal Science Congress

Theme

Strive toward Progress on Sustainable Animal Production Contribute to Environment andWelfare for Human and Livestock

President

Mitsuhiro FURUSE (Professor, Animal & Marine Bioresource Sciences, Kyushu University)

Date

22-25 August. 2016

Venue

Kyushu Sangyo Universityhotel nikko fukuoka

Official Website

http://www.aaap2016.jp/

JAPANESE SOCIETY OFANIMAL SCIENCE (JSAS)

201, Nagatani Corporas, Ikenohata 2-9-4, Taito-ku, Tokyo 110-0008, JapanFAX:+81-(0)3-3828-7649 I E-mail: supportsjsas-orgjp

Secretariat for AAAP2016

clo Convention Linkage, Inc.2 Sanbancho, Chiyoda-ku, Tokyo 102-0075, JapanTEL:+81-(0)3-3263-8695 I E-mail: aaap2016@c-linkage.cojp

3

i~)Animal..Science..ongress

The 17th Asian-Australasian Association of Animal Production Societies Animal Science Congress

Acknowledgements

Supporting Organizations

Association of Japanese Agricultural Scientific SocietiesFukuoka City

Fukuoka PrefectureFukuoka Veterinary Medical Association

Hokkaido Society of Livestock and Grassland ScienceHokushinetsu Society of Animal Science

Japan Embryo Transfer SocietyJapan Ethological Society

Japan Poultry Science AssociationJapan Society for Immunology of ReproductionJapan Society of Reproductive Endocrinology

Japan Veterinary Medical AssociationThe Japanese Society of Animal Breeding and Genetics

Japanese Society for Applied Animal BehaviourJapanese Society of livestock management

The Japanese Society of Swine ScienceThe Japanese Society of Veterinary Science

Kansai Animal Science SocietyKanto Society of Animal Science

Kyushu Sangyo UniversityKyushu University.

SCIENCECOUNCIL OF JAPANSociety of Beef Cattle Science

The Society for Reproduction and DevelopmentTohoku Animal Science and Technology Society

TOKAI SOCIETYOF ANIMAL PRODUCTIONWarm Regional Society of Animal Science, Japan

Foundations

Fukuoka PrefectureFukuoka City

The Ito FoundationJAPAN SOCIETYFORTHE PROMOTION OF SCIENCE

l~liJf ~KAKENHI

Grant-in-Aid for Publication oi Scientitic Research Results (JP76HP0305)

4

~The 17th Asian-Australasian Association of Animal Production Societies Animal Science Congress .•••••----------------------------------- Animal

ScienceCOngress

Sponsors ,

Silver Sponsor

National Federation of Agricultural Cooperative Associations (ZEN-NOH)

~ZEN-NOH

Bronze Sponsor

The National Federation of Dairy Co-operative AssociationsNH Foods Ltd.

NIPPON ZENYAKU KOGYO CO., LTD.Sumitomo Chemical Co., Ltd.

YPTECHCO., LTD.

~~~~!:'~~~:.~.!.,!:!,...-..Nipponham ZENOAQ+ 5UMiTOMO CHEMICAL Y:: YPTECH CO., LTD.

Exhibitors / Advertisements / Contributions

Reprolife CorporationFaculty of engineering Department of applied chemistry and biochemistry Kyushu Sangyo University

Meiji Seika Pharma Co., Ltd.

Sermas co., ltd.

Kyushukyodosyokuniku Co. Ltd.

Japan Racing AssociationAlltech Japan

WILEYAllin One, Inc.

Evonik Japan Co., Ltd.Morinaga Milk Industry Co., Ltd.

Den-en Shuzo Co.,Ltd.Hombo Shuzo Co., Ltd.KIRISHIMA SHUZO Co.,Ltd.Komasa Jyozo Co.,Ltd

Mizuhogiku Shu-zo Co.Taikai Shuzo Co., Ltd.Unkai Shuzo CO.,Ltd.YACHIYODENSYUZOU CO. LTD.

5

~The 17th Asian-Australasian Association of Animal Production Societies Animal Science Congress ...,.-----------------------------------------=-- Animal

ScienceCongre ••

Listof collaborators

Fukuoka Prefecture

Osamu IDE

Kazuhiko IMAMURA

Kyushu University

Vishwajit S.CHOWDHURY

Fuminori KAWABATA

Kazuki ISHIBASHI

Yoshihiro ISOZAKI

Motoyuki MAEDA

Hiroyuki MATSUO

Yuiko MORINAGA

Mako NAKAMURA

Yutaka NAKANO

Wataru MIZUNOYA

Masataka SHIMO]O

Tomoki SOH

Hideyuki TAKAHASHI

Shinobu YASUO

Masahiro NAGANO

Seiji NAGASUE

Kazuhisa NAKAMURA

Sumiko NATSUAKI Kyushu Sangyo University

Koichi ANDOHiroaki RYUOU

Seitarou SHIKITA

Hitomi SHIRAKA WA Ministry of Agriculture, Forestry and Fisheries

Keiji FUSHIMITomitarou TAKATA

Katsuyuki YAMASHITA

Fukuoka Veterinary Medical Association

Kazuto HIRAYAMA

Sei KURASHIGE

Isao KURAUCHI

Haruo KUSABA

Takashi NOHARA

University of Miyazaki

Seiji IEIRI

Yasuyuki ISHII

Genki ISHIGAKI

Kagoshima University

Yoshitaka NAKANISHI

Ichiro OSHIMA

Tetsuo MORITA

Tomonori NAKANISHI

Toshihiro TAKAHASHI

Manabu TOBISA

Tadaaki TOKUNAGA

Yasuhiro TSUZUKI

Koji TAKAYAMA University of the Ryukyus

Yoshimi IMURA

alphabetical order / titles omitted

27

~The 17th Asian-Australasian Association of Animal Production Societies Animal Science Congress ~--------------------------------------------=-- Animol

ScienceCongress

Oral Session33: Animal Nutrition (Ruminants) (3)Thursday, 25 August 11 :00-13:00 Room N302

Chair: Pin Chanjula Prince of Songkla Unversity

0-33-1 In vitro digestibility of fermented rice straw supplemented with cassava tuber and leavesusing ruminal fluid of Bali cattleCuk Tri Noviandi, Zazin Mukmila, Ristianto Utomo, Subur Priyono Sasmito Budhi, Ali Agus,Andriyani Astuti

Faculty of Animal Science, Universitas Gadjah Mada, Indonesia

0-33-2 Rumen contents from slaughter house as alternative feed for replacing forage in ruminantdietsRistianto Utorno', Lies Mira Yusiati', Cuk Tri Noviandi', Aryoqi', lsnandar'

1 Faculty of Animal Science, Universitas Gadjah Mada, Indonesia, 21ndonesian Beef Cattle Research Station, Indonesia,3 National Animal Husbandry Training Center, Indonesia

0-33-3 Influences of Parts of Frond Ensilage and Pelleting Methods on Chemical Physical andDegradability of Pellet Ensiled Oil Palm FrondOngarge Insung

Rajamangala University of Technology Srivijaya

0-33-4 Voluntary feed intake, rumen fermentation and microbial protein synthesis of beef cattlefed fermented cassava starch residueRuangyote Pilajun 1

1 Department of Animal Science, Faculty of Agriculture, Ubon Ratchathani University, Thailand,2Department of Animal Science, Faculty of Agriculture, Khon Kaen University, Thailand

0-33-5 The influence of sowing date and trellising on the flowering of some promising herbaceouslegumes for eastern IndonesiaJacob Nulik'", Evert Hosanq", Debora Kana Haul, Yanto l.iunokas/, Yendri Aby'. Yakobus Urarr',Kendrick Cox'

1 Department of Agriculture and Fisheries (Queensland),2Departemen Pertanian Balai Pengkajian Teknologi Pertanian-NTI (BPTP-NTI)

0-33-6 BIOCONVERSION OF COCOA POD HUSK THROUGH FERMENTATION WITH MOL RUMENTCONTENT AS RUMINANT FEEDNurhaita Nurhaita, Definiati Neli, Suliasih Suliasih

Muhammadiyah University of Bengkulu

0-33-7 Nutrients Quality of Fermented Complete Feed Based on By-Product of Sago (Metroxylon sp.)and Cassava (Mannihot esculenta Cranz)Masitah Siti, Utomo Ristianto, Noviandi Cuk Tri

Faculty of Animal Science Gadjah Mada University

0-33-8 Improving Rice Straw Quality by Treated with Monosodium Glutamate by-Product (MSGB)for Ruminant DietsPhongthorn Kongmun, Sittisak Kongsin, Nuttareakha Polsiri, Somkiert Prasanpanich,Kanokporn Poungpong, Choawit Rakangthong, Chaiyapoom Bunchasak, Wiriya Loongyai,Theerawit Poeikhampa

Department of Animal Science, Faculty of Agriculture, Kasetsart University, Thailand

71

The 17th Asian-Australasian Association of Animal Production Societies Animal Science Congress

712

In vitro digestibility of fermented rice straw supplemented with cassava tuber and leaves using ruminal fluid of Bali cattle

Cuk Tri Noviandi, Zazin Mukmila, Ristianto Utomo, Subur Priyono Sasmito Budhi, Ali Agus, Andriyani Astuti

Faculty of Animal Science, Universitas Gadjah Mada, Indonesia

INTRODUCTIONDigestibility of high fiber feed depends on cellulolytic microbes. In order to maximize the degradation of fibrous feed, the growth of ruminal microbes is needed to be improved. One effort to increase the microbe population in the rumen is by manipulating the adequacy of nutrients for their growth.Microbes in the rumen require energy and protein for their growth and development. A synchronized protein-to-energy ratio indicates optimal fermentation efficiency. Cattle fed only fermented rice straw may be able to provide energy from structural carbohydrates of the rice straw. However, since fermented rice straw also contains rapidly degraded N derived from urea that used in fermentation process, feedstuffs that contain rapidly degraded carbohydrates (non-structural carbohydrates) such as cassava tuber can be used. On the other hand, to synchronize the slowly degraded carbohydrate of rice straw, slow degraded protein source feedstuffs such as cassava leaves can be added. With a good balance of energy and protein availability in the rumen, an increase of microbial population as well as increasing volatile fatty acids (VFA) production in the rumen can be expected.Cassava as a local food commodity in Indonesia is abundant throughout the year. Cassava tuber is known as a good carbohydrate source that is degraded rapidly, while the leaves contain quite high crude protein (CP) which is ranged 20 - 36% (Askar, 1996). The rapidly degraded carbohydrates in cassava tuber and slowly degraded CP in cassava leaves are a good combination as additional feed for cattle fed fermented rice straw. Based on these, a research that focused on improving the in vitro digestibility of fermented rice straw basal feed by supplemented with cassava tubers and leaves was established.

MATERIALS AND METHODSThis research was conducted at the Laboratory of Feed Technology, Department of Nutrition and Feed Science, Faculty of Animal Science, Universitas Gadjah Mada, Yogyakarta, Indonesia. The experimental design used in the study was a 2×2 factorial experimental design, with 2 levels of cassava tuber (without or with 5% cassava tuber) and 2 levels of cassava leaves (without or with 5% cassava leaves). Fresh cassava (Manihot utilisima) tuber and leaves were collected from Gunungkidul area, while fresh rice straw was gathered from Sleman area, Yogyakarta. Fresh cassava tuber and leaves were dried under direct sunlight, and then ground using hammer mill with 1 mm screen. The probiotic used for rice straw fermentation was Starbio® at a dose of 0.3% of dry matter (DM) of rice straw with probiotic-to- urea ratio was 1: 2 (1 g probiotic + 2 g urea/kg DM). After mixed with probiotic and urea mixture, rice straw was stored in the room temperature for 3 weeks.The chemical compositions of samples were analyzed by proximate analysis according to (AOAC, 2005). The chemical compositions of fermented rice straw, cassava tuber and leaves are presented in Table 1. The in vitro digestibility analysis was carried out using two-stage in vitro method (Tilley and Terry, 1963). The data were analyzed by one-way analysis of variance (ANOVA) using SPSS ver. 22 (IBM, USA). Comparisons of means for treatments were done by contrast test with Duncan’s new multiple range tests (Gomez and Gomez, 1984) when the effects of treatments (P ≤ 0.05) were detected.

RESULTS AND DISCUSSSIONThe results of supplementing cassava leaves and tuber in fermented rice straw on in vitro digestibility of DM, organic matter (OM), and CP are presented in Table 2.Dry matter and OM digestibilities (DMD and OMD) of fermented rice straw were increased (P <0.05) due to cassava tubers supplementation, while no significant effect was detected on CP digestibility (Table 2). Cassava contains non-structural (62.5%) and structural carbohydrates, which includes 2.69% cellulose, 0.36% hemicellulose, and 0.02% lignin (Arnata, 2009). Therefore, by adding cassava tuber in the diet would increase the soluble carbohydrates availability in feed. Since soluble carbohydrates is rapidly fermented in rumen, the volatile

O-33-1

The 17th Asian-Australasian Association of Animal Production Societies Animal Science Congress

713

fatty acids (VFA) and adenosine triphosphate (ATP) production from this fermentation may increase, thus more energy is available for microbial growth which lead to greater microbes population in the rumen. Furthermore, the increasing soluble carbohydrates from cassava tuber matched with rapidly degraded N of urea contained in the fermented rice straw, thus the rumen microbes population might be increased significantly. With greater microbe population in the rumen, feed can be degraded more efficient which ended with greater DMD and OMD.Compared with controls, the digestibilities of DM, OM, and CP of fermented rice straw were increased (P <0.05) due to cassava leaves supplementation (Table 2). The digestibility improvement of fermented rice straw due to cassava leaves supplementation were caused by increasing amount of protein available in the feed. In this study, CP content of dried cassava leaves was 30.6%, which was greater than the other reports (Inthapanya et al., 2012 Kiyothong and Wanapat, 2003). Furthermore, the cassava leaves supplementation (as slow degraded protein source) also matched with structural carbohydrates of fermented rice straw, which also leads to increasing population of rumen microbes and then resulted in greater in vitro digestibility of the diet.A positive interactions on DM, OM, and CP digestibilities due to cassava tuber and leaves supplementation were noticed (P<0.05 Table 2) with the best effects was shown in the T1× L1 (34.0, 45.2, and 34.5%, respectively). These results was achieved due to the soluble carbohydrates of cassava tuber matched with the urea from fermented rice straw while the slowly degraded protein of cassava leaves matched with the structural carbohydrate of fermented rice straw. These combinations created a good balance between energy and protein availability in the rumen for microorganisms growth. Previous researcher reported that adding rapidly degraded carbohydrates and protein feedstuffs improved growth of rumen microbes (Kurniawati, 2007).

CONCLUSIONSupplementing cassava tubers and leaves on fermented rice straw in this study showed positive effects on the in vitro digestibility of fermented rice straw with the best combination was at 5% cassava tuber and 5% cassava leaves.

KEYWORD：In vitro digestibility, Fermented rice straw, Cassava tuber, Cassava leaves, Supplementation

Table 1. Chemical compositions of fermented rice straw, cassava tuber and leaves (%)

Feedstuffs Dry matter Organic matter

Crude protein Crude fiber Ether

extract Fermented rice straw 92.9 79.7 8.20 32.4 1.70 Cassava tuber 86.1 97.0 3.11 2.24 0.63 Cassava leaves 87.0 93.4 30.6 24.2 4.87

The 17th Asian-Australasian Association of Animal Production Societies Animal Science Congress

714

REFERENCEAOAC. 2005. Official Methods of Analysis. 18th ed. AOAC International, Gaithersburg, MD.Arnata, I. W. 2009. Teknologi bioproses pembuatan bioetanol dari ubi kayu (Manihot utilisima) menggunakan

kultur campuran Trichoderma viride, Aspergillus niger dan Saccharomyces cerevisiae, Institut Pertanian Bogor, Bogor.

Askar, S. 1996. Daun singkong dan pemanfaatannya terutama sebagai pakan tambahan. Wartazoa 5: 21-25.Gomez, K. A., and A. A. Gomez. 1984. Statistical Procedures for Agricultural Research. 2nd ed. John Wiley & Sons,

New York.Inthapanya, S., T. R. Preston, D. N. Khang, and R. A. Leng. 2012. Effect of method of processing of cassava leaves

on protein solubility and methane production in an in vitro incubation using cassava root as source of energy. Livest. Res. Rural Dev. 24.

Kiyothong, K., and M. Wanapat. 2003. Cassava hay and Stylo 184 hay to replace concentrates in diets for lactating dairy cows. Livest. Res. Rural Dev. 15.

Kurniawati, A. 2007. Teknik produksi gas in vitro untuk evaluasi pakan ternak: Volume produksi gas dan kecernaan bahan pakan. Jurnal Ilmiah Aplikasi Isotop dan Radiasi 3: 40-49.

Tilley, J. M. A., and R. A. Terry. 1963. A two-stage technique for the in vitro digestion of forage crops. Grass Forage Sci. 18: 104-111.

Table 2. In vitro digestibility of fermented rice straw supplemented with cassava leaves and tuber

Leaves (% as fed)2 Tuber (% as fed)1 Means T0 T1

Dry matter digestibility (%) L0 22.8p 27.4q 25.1x

L1 30.5r 34.0s 32.3y

Means 26.7a 30.7b

Organic matter digestibility (%) L0 36.0p 41.1q 38.6x

L1 44.1qr 45.2r 44.7y

Means 40.1a 43.2b

Crude protein digestibility (%)

L0 25.5p 26.5p 26.0x

L1 33.1q 34.5q 33.8y

Means 29.3 30.5 a,b Means in the same row within same group with different superscripts differ at P<0.05.

x,y Means in the same column within same group with different superscripts differ at P<0.05. p,q,r,s Means in the same row and column within same group with different superscripts differ at P<0.05. 1T0= fermented rice straw without cassava tuber; T1= fermented rice straw with 5% cassava tuber. 2L0= fermented rice straw without cassava leaves; L1= fermented rice straw with 5% cassava leaves.

C E RT I F I CAT E O F PRESENTAT ION

This is to certify that

Cuk Tri NoviandiZaz\n MukmilaRistianto Utomo

Subur Priyono Sasmito BudhiAti Agus

Andriyani Astuti

made an oral presentation on the following paper at

the rTth Asia-Australasian Association of

Animal Production Society Animal Science Congress

held in Fukuoka, fapan from zz to z5 August zo16

In vitro digestibility of fermented rice stra\ r supplemented

with cassava tuber and leaves using ruminal fluid of Bali

cattle(o-r-r)

0/fuMitsuhiro Furuse, Ph.D.

President of the rTth Ar{.,{P Animal Science Congress