artifacts - escmid

Center of Infectious Diseases Department of Parasitology

Artifacts <hand-out without pictures>

LUMC - c

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paras

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ESCMID Online Lecture Library

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Fecal elements

- Nice to examine - Very interesting material - Many things to see - Every stool sample is different

Feces

What can we see in a stool sample ? LUMC - c

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Parasites ?

Mite eggs Like hookworm eggs, only larger Hookworm egg 65 x 40 µm Trichostrongylus egg 100 x 75 µm Mite egg 140 x 90 µm In organic material (food - cheese, flour, dust)

LUMC - c

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Parasites ?

Which egg….. ??

140 x 80 µm

And more important: Infection or not ?

Fasciola hepatica

- Fasciola is common in sheep and cows

- Eating liver

- Ask new stool sample LU

MC - cou

rse pa

rasito

logy


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Artifacts

Intestinal protozoa ?

Yeasts and fungi

- Strange forms

- Different sizes

LU

MC - cou

rse pa

rasito

logy


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Artifacts

Fungal spores (Empty and different sizes) LU

MC - cou

rse pa

rasito

logy


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Artifacts

Cysts of protozoa ?

Spores of Mushrooms - Empty

- Lying in a row or package

- Cyst wall is hard and thick

LU

MC - cou

rse pa

rasito

logy


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Artifacts

Entamoeba histolytica ?

• Leukocytes, macrophages

• Epithelial cells

• Look for the typical Entamoeba nuclei

(karyosome and peripheral chromatin)

LUMC - c

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paras

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Artifacts

Pollen

Looks like worm eggs !

LUMC - c

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paras

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Artifacts

Various forms,

often with ridges, spines or pores

(usually between 15-50 µm) LUMC - c

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paras

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Artifacts

You will find them often in your slide :

Smooth edge, very thick and dark Air bubbles

LUMC - c

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paras

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Artifacts

Cestode egg ?

LUMC - c

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paras

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Parasites ?

Free living nematodes

- Adults and/or larvae

- Different species and sizes

- Strange tail or buccal cavity

- In soil, food (often plant nematodes)

Larva of which parasite ?

LUMC - c

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paras

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Artifacts

Plant hairs

- Thick smooth wall

- No internal structures (oesophagus, intestine etc.)

- Look like larvae

Again larvae ?

LUMC - c

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paras

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Artifacts

Packed cells from a drain Ascaris ?

LUMC - c

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paras

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© by author


Artifacts

Earthworm : - Hermaphrodite (Saddle) - No lips (Ascaris 3 lips) - Broader segments

www.welcomewildlife.com

salinella.bio.uottawa.ca

LUMC - c

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paras

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http://www.welcomewildlife.com/

https://www.google.nl/url?sa=i&rct=j&q=&esrc=s&source=images&cd=&cad=rja&uact=8&ved=0CAYQjB0&url=http://salinella.bio.uottawa.ca/bio2135/labs/defaultPrint.php?pofthep/2007/lab07_nemacrus/lab07_nemacrus.htm&ei=PI-9VLvtN4T0PJmJgZAB&bvm=bv.83829542,d.ZWU&psig=AFQjCNF6d_iRmcC7bL1ZSgRBHBlSmnc_mg&ust=1421795297572825


Artifacts

color earthworm more red / brown (dead earthworm : more yellow / white)

We receive more earthworms than Ascaris worms

LUMC - c

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paras

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© by author


Artifacts

- Baby 6 months old - Very sick - Vomiting - During the night - Parents came found a worm

Case - Crying baby

- They went to the general practitioner - It's definitely a worm - General practitioner called LUMC : how do

I have to treat this - LUMC : better bring the worm for

diagnosis LUMC - c

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paras

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© by author


Artifacts

Woman comes with a piece of kitchen paper with ........ parasites!

-Several weeks parasites in the stool

-With self made pictures of the parasites

Case 2

LUMC - c

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paras

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