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TRANSCRIPT
Supplementary material
Enzyme-catalyzed simultaneous hydrolysis-glycolysis reactions reveals
tunability on PET depolymerization products
Aline M. de Castroa*, Adriano Carnielb, Lys Sirelli c, Marcos L. Dias c, Sonia Maria Cabral de
Menezes d, Luiz Silvino Chinelatto Junior d, Hercílio de Angeli Honorato d
a Biotechnology Division, Research and Development Center (Cenpes), PETROBRAS,
Av. Horácio Macedo, 950, 21941-915. Rio de Janeiro, Brazil.
b Falcão Bauer. R. Aquinos, 111. Água Branca, São Paulo, 05036-070, Brazil.
c Institute of Macromolecules Professor Eloisa Mano (IMA), Federal University of Rio
de Janeiro (UFRJ). Av. Horácio Macedo, 2030, 21941-598. Rio de Janeiro, Brazil.
d Chemistry Division, Research and Development Center (Cenpes), PETROBRAS,
Av. Horácio Macedo, 950, 21941-915. Rio de Janeiro, Brazil.
* Corresponding author. E-mail: [email protected]
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Fig. 1S. DSC curves of PC-PET and oligomers O1, O2 and O3 (First heating run).
Fig. 2S. DSC curves of PC-PET and oligomers O1, O2 and O3 (Second heating run).
2
Fig. 3S. DSC curves of PC-PET and oligomers O1, O2 and O3 (Second cooling run).
Fig. 4S. TG curves of PC-PET and oligomers O1, O2 and O3.
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Fig. 5S. SEM images of (a) O1, (b) O2 and (c) O3 particles before the reactions. Scale bars
correspond to 20 µm.
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(c)
(b)(a)
Figure 6S. T2 relaxation curves obtained by CPMG sequence of oligomers O1, O2 and O3,
and PC-PET.
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Normalized intensity
Time (ms)
Fig. 7S. Time course of TPA (a), MHET (b) and BHET (c) concentrations during
simultaneous hydrolysis-glycolysis reactions of PC-PET catalyzed by HiC. Reactions were
carried out at 62 °C and 0.02 gprotein/gPET. Different added EG contents were investigated: 0%
(closed squares), 20% (closed circles), 40% (closed triangles), 60% (open squares), 80%
(open circles) and 100% (open triangles).
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0
10
20
30
40
50
60
70
80
90
100
0 2 4 6 8 10 12 14 16
TPA
Con
cent
ratio
n (m
M)
Reaction time (days)
A
0.00
0.05
0.10
0.15
0.20
0.25
0.30
0.35
0 2 4 6 8 10 12 14 16
BH
ET C
once
ntra
tion
(mM
)
Reaction time (days)
C
0
5
10
15
20
25
30
35
40
45
0 2 4 6 8 10 12 14 16
MH
ET C
once
ntra
tion
(mM
)
Reaction time (days)
B
0
10
20
30
40
50
60
70
80
90
100
0 2 4 6 8 10 12 14 16
TPA
Con
cent
ratio
n (m
M)
Reaction time (days)
A
0.00
0.05
0.10
0.15
0.20
0.25
0.30
0.35
0 2 4 6 8 10 12 14 16
BH
ET C
once
ntra
tion
(mM
)
Reaction time (days)
C
0
5
10
15
20
25
30
35
40
45
0 2 4 6 8 10 12 14 16
MH
ET C
once
ntra
tion
(mM
)
Reaction time (days)
Bba
c
0%
10%
20%
30%
40%
50%
60%
70%
80%
90%
100%
0 2 4 6 8 10 12 14 16
ED
Reaction time (days)
Fig. 8S. Esterification degree (ED) of the reaction products mixture during simultaneous
hydrolysis-glycolysis reactions. Different added EG contents were investigated: 0% (closed
squares), 20% (closed circles), 40% (closed triangles), 60% (open squares), 80% (open
circles) and 100% (open triangles).
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Fig. 9S. DSC curves of PC-PET and residual PET samples depolymerized with added EG
percentages from 0 to 100% (First heating run).
Fig. 10S. DSC curves of PC-PET and residual PET samples depolymerized with added EG
percentages from 0 to 100% (Second heating run).
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Fig. 11S. DSC curves of PC-PET and residual PET samples depolymerized with added EG
percentages from 0 to 100% (Second cooling run).
Fig. 12S. TG curves of PC-PET and residual PET samples depolymerized with EG
percentages from 0 to 100%.
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