april 3, 2008 · web viewtitle: assessing impact of algal species indigenous to oil sands...
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Title: Assessing impact of algal species indigenous to oil sands process-affected waters on mixtures of oil sands naphthenic acids
Authors:
Sarah E. Ruffella, Richard A. Franka, Adam P. Woodwortha, Anthony E. Bauera, Leslie M. Bragga, Lorna E. Deethb, Kirsten M.
Müllera, Andrea J. Farwella, D. George Dixona, Mark R. Servosa, and Brendan J. McConkeya*
aDepartment of Biology, University of Waterloo, Waterloo, ON, Canada N2L 3G1
bDepartment of Mathematics & Statistics, University of Guelph, Guelph, ON, Canada
N1G 2W1
*Corresponding Author: Brendan J. McConkey
Department of Biology
University of Waterloo
Waterloo, ON, Canada N2L 3G1
Phone: (519)888-4567 x37020
Fax: (519)746-0614
Email: [email protected]
SUPPORTING INFORMATION:
Table S1: Slopes of linear regressions of "all ion" and "marker ion" data at each test concentration (Lower, Upper 95% CI)
Stichococcus sp. 1 Scenedesmus sp. 1All ions Marker ions All ions Marker ions
No. of characterized ions 209 9 209 17
Control 11000* (5570, 16400) 75.3 (-18.9, 170) 4630 (2900, 6360) -106 (-409, 197)p-value 0.00489 0.0907 0.00176 0.385
10 mg L-1 1970 (-4000, 7940) -2160* (-3810, -509) 7600 (-3230, 18400) 1940 (-4750, 8630)p-value 0.412 0.0221 0.123 0.465
30 mg L-1 -1830 (-4660, 997) -1960* (-3290, -623) 8500 (343, 16700) 3700 (-6270, 13700)p-value 0.147 0.0152 0.0444 0.361
100 mg L-1-7160 (-20300, 6020)
-4080* (-7707.456, -456.544) 1800 (-5020, 8620) -1650 (-5450, 2160)
p-value 0.206 0.0353 0.505 0.296Oocystis sp. 1 Chlorella sp. 1
All ions Marker ions All ions Marker ionsNo. of
characterized ions 209 5 209 5Control 2600 (-1140, 6340) 11.6 (-59.7, 82.8) 2380 (1150, 3610) n/ap-value 0.126 0.676 0.00587 n/a
10 mg L-1 6040 (-16200, 28300) 350 (-2660, 3360) 956 (-4050, 5960) -15.3 (-705, 675)p-value 0.493 0.763 0.624 0.954
30 mg L-1 16100 (2170, 30000) 331 (-271, 933) -2010 (-7670, 3650) -127 (-930, 676)p-value 0.0326 0.201 0.380 0.684
100 mg L-1 -4140 (-19300, 11000) -1200 (-3830, 1400) 269 (-4130, 4670) -114 (-919, 691)p-value 0.490 0.275 0.873 0.714
Notes: " * " denotes slope significantly different from 0 (α = 0.05); " n/a " indicates insufficient marker ion data for regression analysis
Table S1 (cont.): Slopes of linear regressions of "all ion" and "marker ion" data at each test concentration (Lower, Upper 95% CI)Aphanocapsa sp. 1 Euglena sp. 1
All ions Marker ions All ions Marker ionsNo. of
characterized ions 209 6 209 6Control 5750 (1930, 9570) -9.08 (-43.3, 25.2) 6701* (3700, 9700) 0 (n/a)p-value 0.0139 0.503 0.00344 n/a
10 mg L-1 3260 (510, 6010) 131 (-401, 663) 4150* (1920, 6390) 67.9 (-373, 509)p-value 0.0302 0.533 0.00672 0.691
30 mg L-1 -1150 (-5370, 3070) -118 (-471, 236) 2440 (-177, 5060) -29.5 (-300, 241)p-value 0.492 0.407 0.0608 0.777
100 mg L-1 -2330 (-5430, 758) -107 (-598, 384) -182 (-3070, 2710) 38.7 (-192, 270)p-value 0.104 0.577 0.870 0.666
Anabaena sp. 1 Monoraphidium sp. 1All ions Marker ions All ions Marker ions
No. of characterized ions 209 3 209 7
Control 381 (-122, 883) n/a 3380 (3020, 3740) n/ap-value 0.103 n/a 0.0000130 n/a
10 mg L-1 -1210 (-4480, 2070) -362 (-1310, 585) 3094 (361.4, 5830) 312 (-405, 1030)p-value 0.364 0.349 0.0347 0.294
30 mg L-1 -231 (-2630, 2170) -20.7 (-195, 153) 3540 (-359, 7440) 1480 (141, 2820)p-value 0.803 0.758 0.0653 0.0374
100 mg L-1 2110 (-846, 5070) -62.1 (-505, 381) 1340 (382, 2300) 152 (-973, 1280)p-value 0.119 0.717 0.0179 0.727
Notes: " * " denotes slope significantly different from 0 (α = 0.05); " n/a " indicates insufficient marker ion data for regression analysis
Table S1 (cont.): Slopes of linear regressions of "all ion" and "marker ion" data at each test concentration (Lower, Upper 95% CI)Scenedesmus sp. 3 Monoraphidium sp. 2
All ions Marker ions All ions Marker ionsNo. of
characterized ions 209 21 209 22Control -864 (-2930, 1200) -44.7 (-171, 81.2) 2040 (752, 3330) 3.96 (-138, 146)p-value 0.310 0.381 0.0118 0.942
10 mg L-1 -740 (-4420, 2940) -175 (-1860, 1510) 2190 (-2020, 6390) -1070 (-2690, 550)p-value 0.607 0.787 0.222 0.141
30 mg L-1 -346 (-4610, 3920) -134 (-3260, 2990) 3840 (79.7, 7610) 91.5 (-2020, 2200)p-value 0.833 0.910 0.0471 0.910
100 mg L-1 1110 (-6330, 8540) -22.5 (-3280, 3240) 9020 (-7200, 25000) 4520 (-8300, 17300)p-value 0.700 0.986 0.197 0.383
Oocystis sp. 2 Unidentified sp. 1All ions Marker ions All ions Marker ions
No. of characterized ions 209 14 209 3
Control 159 (-2190, 2510) -47.1 (-260, 166) 3890 (2280, 5500) -16.2 (-104, 71.4)p-value 0.860 0.573 0.00255 0.635
10 mg L-1 -567 (-14000, 12900) -2040 (-10300, 6210) -127 (-11700, 11400) 368 (-367, 1100)p-value 0.912 0.530 0.977 0.237
30 mg L-1 1390 (-4650, 7420) -288 (-1970, 1390) -1200 (-10950, 8550) -40.4 (-1350, 1270)p-value 0.558 0.659 0.750 0.936
100 mg L-1 1640 (-6280, 9570) 1210 (-2150, 4570) -1120 (-16300, 14000) -325 (-1800, 1150)p-value 0.596 0.374 0.847 0.575
Notes: " * " denotes slope significantly different from 0 (α = 0.05); " n/a " indicates insufficient marker ion data for regression analysis
Table S1 (cont.): Slopes of linear regressions of "all ion" and "marker ion" data at each test concentration (Lower, Upper 95% CI)Tetraedron sp. 1 Ulothrix sp. 1
All ions Marker ions All ions Marker ionsNo. of
characterized ions 209 21 209 12Control 2380 (586, 4180) 6.67 (-207, 220) 2390 (-1100, 5870) 84.9 (-54.3, 224)p-value 0.0212 0.935 0.130 0.166
10 mg L-1 117 (-21800, 22000) -794 (-10400, 8800) 1050 (-3660, 5770) -466 (-2200, 1270)p-value 0.989 0.830 0.569 0.497
30 mg L-1 7540 (-13200, 28300) 3480 (-8580, 15500) 1220 (-5710, 8150) -240 (-3630, 3160)p-value 0.370 0.468 0.651 0.854
100 mg L-1 2610 (-8460, 13700) 3160 (-7260, 13600) 640 (-9240, 10500) 490 (-1200, 2180)p-value 0.549 0.448 0.866 0.467
Scenedesmus sp. 4 Chlorella sp. 3All ions Marker ions All ions Marker ions
No. of characterized ions 209 15 209 19
Control 4800 (-1790, 11400) 25.1 (-339, 389) -2330 (-7440, 2790) -704 (-1840, 430)p-value 0.113 0.858 0.275 0.160
10 mg L-1 6130 (-2500, 14800) 1500 (-2700, 5700) -2980 (-10200, 4240) -4700 (-10400, 1020)p-value 0.120 0.376 0.316 0.0846
30 mg L-1 3110 (-4130, 10300) 252 (-2990, 3500) -9330 (-34100, 15400) -5990 (-19600, 7610)p-value 0.299 0.840 0.355 0.288
100 mg L-1 7990* (2220, 13800) -191 (-5470, 5090) 1730 (-8200, 11700) 193 (-6900, 7280)p-value 0.0184 0.925 0.654 0.944
Notes: " * " denotes slope significantly different from 0 (α = 0.05); " n/a " indicates insufficient marker ion data for regression analysis
Table S1 (cont.): Slopes of linear regressions of "all ion" and "marker ion" data at each test concentration (Lower, Upper 95% CI)Unidentified sp. 2
All ions Marker ionsNo. of
characterized ions 209 12Control -434 (-2100, 1240) 67.8 (-44.1, 180)p-value 0.510 0.168
10 mg L-1 1770 (-4650, 8190) -82.8 (-25240 2360)p-value 0.487 0.930
30 mg L-1 3660 (-5560, 12900) 1060 (-1960, 4080)p-value 0.332 0.385
100 mg L-1 1220 (-1820, 4250) 325 (-1870, 2520)p-value 0.328 0.703
Notes: " * " denotes slope significantly different from 0 (α = 0.05); " n/a " indicates insufficient marker ion data for regression analysis
Figure Captions:
Figure S1: Linearity of the concentration-response from 1 mg L-1 to 75 mg L-1 for a previously characterized naphthenic acid aliquot (Rogers et al., 2002). (A) total ion abundance; (B) marker ion abundance.
Figure S2: Summation of MS peak areas for “All ion” (solid circle) and “Marker ion” (open circle) approaches for 17 algal bioremediation candidate strains. The “All ion” approach characterized and summed the MS peak areas for NAs representing the 209 previously reported “classical NAs” (Lo et al., 2003). The “Marker ion” approach characterized and summed the MS peak areas for the masses identified as being present within the NA extract yet not having interference from biogenic acids released by the phytoplankton strains. Linear regressions were modeled for “All ion” and “Marker ion” approaches using R (R Development Core Team, 2008) with b1 representing the slope of the regression lines.
Figure S1
0 10 20 30 40 50 60 70 800
100000
200000
300000
400000
500000
600000
700000
800000
900000
1000000
f(x) = 11651.5445620159 x + 10390.6651030872R² = 0.999838161399935
A
Concentration, mg L-1
Tota
l Ion
Abu
ndan
ce
0 10 20 30 40 50 60 70 800
50000
100000
150000
200000f(x) = 2622.60259626923 x − 1186.00359986909R² = 0.992918197447135
B
Concentration, mg L-1
Mar
ker I
on A
bund
ance
Figure S2
Sample Day
0 2 4 6 8 10 12 14
MS
Pea
k A
rea
0
105
2.0x105
3.0x105
4.0x105
5.0x105
0 2 4 6 8 10 12 14
MS
Pea
k A
rea
0
105
2.0x105
3.0x105
4.0x105
0 2 4 6 8 10 12 14
MS
Pea
k A
rea
0
105
2.0x105
3.0x105
4.0x105
0 2 4 6 8 10 12 14
MS
Pea
k A
rea
0
5.0x104
105
1.5x105
2.0x105
2.5x105
100 mg L -1 NA
30 mg L -1 NA
10 mg L -1 NA
Control
b1 = -4081.5
b1 = -7156.1
b1 = -1955.4
b1 = -1831.8
b1 = -2158.2
b1 = 1967.2
b1 = 75.3
b1 = 10987.2
Stichococcus sp.1
Sample Day
0 2 4 6 8 10 12 14
MS
Pea
k A
rea
0
105
2.0x105
3.0x105
4.0x105
5.0x105
0 2 4 6 8 10 12 14
MS
Pea
k A
rea
0
105
2.0x105
3.0x105
4.0x105
5.0x105
0 2 4 6 8 10 12 14
MS
Pea
k A
rea
0
105
2.0x105
3.0x105
4.0x105
5.0x105
0 2 4 6 8 10 12 14
MS
Pea
k A
rea
0
2.0x104
4.0x104
6.0x104
8.0x104
105
1.2x105
1.4x105
100 mg L -1
30 mg L -1
10 mg L -1
Control
b1 = -
b1 =
b1 =
b1 =
b1 =
b1 =
b1 = -
b1 =
Scenedesmus sp.1
Sample Day
0 2 4 6 8 10 12 14
MS
Pea
k A
rea
0
105
2.0x105
3.0x105
4.0x105
5.0x105
6.0x105
0 2 4 6 8 10 12 14
MS
Pea
k A
rea
0
105
2.0x105
3.0x105
4.0x105
5.0x105
6.0x105
7.0x105
0 2 4 6 8 10 12 14
MS
Pea
k A
rea
0
105
2.0x105
3.0x105
4.0x105
5.0x105
0 2 4 6 8 10 12 14
MS
Pea
k A
rea
0
5.0x104
105
1.5x105
2.0x105
Control
Oocystis sp. 1
100 mg L -1 NA
30 mg L -1 NA
10 mg L -1 NA
b1 = -1197.5
b1 = -4136.1
b1 = 331.0
b1 = 16062.4
b1 = 350.0
b1 = 6042.5
b1 = 11.6
b1 = 2599.4
Sample Day
0 2 4 6 8 10 12 14
MS
Pea
k A
rea
0
5.0x104
105
1.5x105
0 2 4 6 8 10 12 14
MS
Pea
k A
rea
0
5.0x104
105
1.5x105
2.0x105
2.5x105
0 2 4 6 8 10 12 14
MS
Pea
k A
rea
0
5.0x104
105
1.5x105
2.0x105
0 2 4 6 8 10 12 14
MS
Pea
k A
rea
0
2.0x104
4.0x104
6.0x104
8.0x104
105
Control
Chlorella sp. 1
100 mg L -1 NA
30 mg L -1 NA
10 mg L -1 NA
b1 = -114.0
b1 = 269.4
b1 = -126.7
b1 = -2012.5
b1 = -15.3
b1 = 955.7
b1 = 0
b1 = 2378.7
Sample Day
0 2 4 6 8 10 12 14
MS
Pea
k A
rea
0
5.0x104
105
1.5x105
2.0x105
0 2 4 6 8 10 12 14
MS
Pea
k A
rea
0
5.0x104
105
1.5x105
2.0x105
0 2 4 6 8 10 12 14
MS
Pea
k A
rea
0
5.0x104
105
1.5x105
2.0x105
0 2 4 6 8 10 12 14
MS
Pea
k A
rea
0
2.0x104
4.0x104
6.0x104
8.0x104
105
1.2x105
1.4x105
1.6x105
Control
Aphanocapsa sp. 1
100 mg L -1 NA
30 mg L -1 NA
10 mg L -1 NA
b1 = -107.0
b1 = -2334.0
b1 = -117.8
b1 = -1148.7
b1 = 130.8
b1 = 3259.7
b1 = -9.1
b1 = 5746.9
Sample Day
0 2 4 6 8 10 12 14
MS
Pea
k A
rea
0
5.0x104
105
1.5x105
2.0x105
0 2 4 6 8 10 12 14
MS
Pea
k A
rea
0
5.0x104
105
1.5x105
2.0x105
0 2 4 6 8 10 12 14
MS
Pea
k A
rea
0
2.0x104
4.0x104
6.0x104
8.0x104
105
1.2x105
1.4x105
1.6x105
1.8x105
0 2 4 6 8 10 12 14
MS
Pea
k A
rea
0
2.0x104
4.0x104
6.0x104
8.0x104
105
1.2x105
1.4x105
1.6x105
Control
Euglena sp. 1
100 mg L -1 NA
30 mg L -1 NA
10 mg L -1 NA
b1 = 38.7
b1 = -181.8
b1 = -29.5
b1 = 2442.9
b1 = 67.9
b1 = 4154.2
b1 = 0
b1 = 6701.0
Sample Day
0 2 4 6 8 10 12 14
MS
Pea
k A
rea
0
2.0x104
4.0x104
6.0x104
0 2 4 6 8 10 12 14
MS
Pea
k A
rea
0
104
2.0x104
3.0x104
4.0x104
5.0x104
6.0x104
0 2 4 6 8 10 12 14
MS
Pea
k A
rea
0
2.0x104
4.0x104
6.0x104
8.0x104
0 2 4 6 8 10 12 14
MS
Pea
k A
rea
0
5.0x103
104
1.5x104
2.0x104
2.5x104
Control
Anabaena sp. 1
100 mg L -1 NA
30 mg L -1 NA
10 mg L -1 NA
b1 = -62.1
b1 = 2110.1
b1 = -20.7
b1 = -230.9
b1 = -361.5
b1 = -1207.2
b1 = 0
b1 = 380.5
Sample Day
0 2 4 6 8 10 12 14
MS
Pea
k A
rea
0
2.0x104
4.0x104
6.0x104
8.0x104
105
1.2x105
1.4x105
0 2 4 6 8 10 12 14
MS
Pea
k A
rea
0
2.0x104
4.0x104
6.0x104
8.0x104
105
1.2x105
1.4x105
0 2 4 6 8 10 12 14
MS
Pea
k A
rea
0
2.0x104
4.0x104
6.0x104
8.0x104
105
1.2x105
1.4x105
0 2 4 6 8 10 12 14
MS
Pea
k A
rea
0
2.0x104
4.0x104
6.0x104
8.0x104
105
Control
Monoraphidium sp. 1
100 mg L -1 NA
30 mg L -1 NA
10 mg L -1 NA
b1 = 151.6
b1 =1343.2
b1 = 1481.9
b1 = 3541.4
b1 = 311.9
b1 = 3093.5
b1 = 0
b1 = 3376.7
Sample Day
0 2 4 6 8 10 12 14
MS
Pea
k A
rea
0
105
2.0x105
3.0x105
4.0x105
5.0x105
0 2 4 6 8 10 12 14
MS
Pea
k A
rea
0
105
2.0x105
3.0x105
4.0x105
0.0 2.0 4.0 6.0 8.0 10.0 12.0 14.0
MS
Pea
k A
rea
0
105
2.0x105
3.0x105
4.0x105
0 2 4 6 8 10 12 14
MS
Pea
k A
rea
0
2.0x104
4.0x104
6.0x104
8.0x104
105
1.2x105 Control
Scenedesmus sp. 3
100 mg L -1
30 mg L -1
10 mg L -1
b1 = -
b1 =
b1 = -
b1 = -
b1 = -
b1 = -
b1 = -
b1 = -
Sample Day
0 2 4 6 8 10 12 14
MS
Pea
k A
rea
0
105
2.0x105
3.0x105
4.0x105
5.0x105
6.0x105
0 2 4 6 8 10 12 14
MS
Pea
k A
rea
0
105
2.0x105
3.0x105
4.0x105
0 2 4 6 8 10 12 14
MS
Pea
k A
rea
0
105
2.0x105
3.0x105
0 2 4 6 8 10 12 14
MS
Pea
k A
rea
0
2.0x104
4.0x104
6.0x104
8.0x104
105
Control
Monoraphidium sp. 2
100 mg L -1
30 mg L -1
10 mg L -1
b1 =
b1 =
b1 =
b1 =
b1 = -
b1 =
b1 =
b1 =
Sample Day
0 2 4 6 8 10 12 14
MS
Pea
k A
rea
0
105
2.0x105
3.0x105
4.0x105
0 2 4 6 8 10 12 14
MS
Pea
k A
rea
0
105
2.0x105
3.0x105
4.0x105
0 2 4 6 8 10 12 14
MS
Pea
k A
rea
0
105
2.0x105
3.0x105
0 2 4 6 8 10 12 14
MS
Pea
k A
rea
0
2.0x104
4.0x104
6.0x104
8.0x104Control
Oocystis sp. 2
100 mg L -1 NA
30 mg L -1 NA
10 mg L -1 NA
b1 = 1209.6
b1 = 1643.7
b1 = -287.9
b1 = 1385.5
b1 = -2042.7
b1 = -567.3
b1 = -47.1
b1 = 159.4
Sample Day
0 2 4 6 8 10 12 14
MS
Pea
k A
rea
0
2.0x105
4.0x105
6.0x105
8.0x105
0 2 4 6 8 10 12 14
MS
Pea
k A
rea
0
2.0x105
4.0x105
6.0x105
0 2 4 6 8 10 12 14
MS
Pea
k A
rea
0
105
2.0x105
3.0x105
4.0x105
5.0x105
6.0x105
0 2 4 6 8 10 12 14
MS
Pea
k A
rea
0
2.0x104
4.0x104
6.0x104
8.0x104
105
1.2x105
1.4x105
1.6x105
Control
Unidentified sp. 1
100 mg L -1 NA
30 mg L -1 NA
10 mg L -1 NA
b1 = -324.8
b1 = -1120.6
b1 = -40.4
b1 = -1198.7
b1 = 367.5
b1 = -127.1
b1 = -16.2
b1 = 3891.0
Sample Day
0 2 4 6 8 10 12 14
MS
Pea
k A
rea
0
105
2.0x105
3.0x105
4.0x105
0 2 4 6 8 10 12 14
MS
Pea
k A
rea
0
105
2.0x105
3.0x105
4.0x105
0 2 4 6 8 10 12 14
MS
Pea
k A
rea
0
105
2.0x105
3.0x105
4.0x105
0 2 4 6 8 10 12 14
MS
Pea
k A
rea
0
2.0x104
4.0x104
6.0x104
8.0x104
105 Control
Tetraedron sp. 1
100 mg L -1 NA
30 mg L -1 NA
10 mg L -1 NA
b1 = 3158.9
b1 = 2604.8
b1 = 3478.5
b1 = 7537.2
b1 = -793.9
b1 = 117.9
b1 = 6.7
b1 = 2380.7
Sample Day
0 2 4 6 8 10 12 14
MS
Pea
k A
rea
0
105
2.0x105
3.0x105
4.0x105
5.0x105
0 2 4 6 8 10 12 14
MS
Pea
k A
rea
0
105
2.0x105
3.0x105
4.0x105
0 2 4 6 8 10 12 14
MS
Pea
k A
rea
0
105
2.0x105
3.0x105
4.0x105
0 2 4 6 8 10 12 14
MS
Pea
k A
rea
0
2.0x104
4.0x104
6.0x104
8.0x104
105
1.2x105
1.4x105
Control
Ulothrix sp. 1
100 mg L -1 NA
30 mg L -1 NA
10 mg L -1 NA
b1 = 489.5
b1 = 640.4
b1 = -239.5
b1 = 1219.1
b1 = -466.2
b1 = 1053.1
b1 = 84.9
b1 = 2387.8
Sample Day
0 2 4 6 8 10 12 14
MS
Pea
k A
rea
0
2.0x105
4.0x105
6.0x105
8.0x105
0 2 4 6 8 10 12 14
MS
Pea
k A
rea
0
2.0x105
4.0x105
6.0x105
0 2 4 6 8 10 12 14
MS
Pea
k A
rea
0
2.0x105
4.0x105
6.0x105
0 2 4 6 8 10 12 14
MS
Pea
k A
rea
0
5.0x104
105
1.5x105
2.0x105 Control
Scenedesmus sp. 4
100 mg L -1
30 mg L -1
10 mg L -1
b1 = -
b1 =
b1 =
b1 =
b1 =
b1 =
b1 =
b1 =
Sample Day
0 2 4 6 8 10 12 14
MS
Pea
k A
rea
0
2.0x105
4.0x105
6.0x105
8.0x105
106
1.2x106
1.4x106
1.6x106
0 2 4 6 8 10 12 14
MS
Pea
k A
rea
0
2.0x105
4.0x105
6.0x105
0 2 4 6 8 10 12 14
MS
Pea
k A
rea
0
2.0x105
4.0x105
6.0x105
0 2 4 6 8 10 12 14
MS
Pea
k A
rea
0
5.0x104
105
1.5x105
2.0x105 Control
Chlorella sp. 3
100 mg L -1 NA
30 mg L -1 NA
10 mg L -1 NA
b1 = 192.5
b1 = 1730.7
b1 = -5993.8
b1 = -9329.6
b1 = -4697.3
b1 = -2978.5
b1 = -704.2
b1 = -2327.8
Sample Day
0 2 4 6 8 10 12 14
MS
Pea
k A
rea
0
5.0x104
105
1.5x105
2.0x105
0 2 4 6 8 10 12 14
MS
Pea
k A
rea
0
5.0x104
105
1.5x105
2.0x105
2.5x105
0 2 4 6 8 10 12 14
MS
Pea
k A
rea
0
5.0x104
105
1.5x105
2.0x105
0 2 4 6 8 10 12 14
MS
Pea
k A
rea
0
2.0x104
4.0x104
6.0x104
8.0x104
Control
Unidentified sp. 2
100 mg L -1
30 mg L -1
10 mg L -1
b1 = -
b1 =
b1 =
b1 =
b1 = -
b1 =
b1 =
b1 = -