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(Bio)nanosystems based on
quantum dots, plasmonic or
magnetic nanoparticles
Paulo J. G. Coutinho
Departamento/Centro de Física
Escola de Ciências
Universidade do Minho
4 Julho 2016
Ciência 2016
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Molecular engineering is continuously being enriched by the design
and exploration of molecular materials at the nanometer scale
Detectable properties
revolution in
OVERVIEW
become tunable by simply controlling its
shape and size
• materials science
• device technology
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NanoparticlesHigh electron density
originating plasmonic
effects (e.g. metal
particles: Au, Ag, …)
Efficient
photoluminescence
(semiconductor quantum
dots, e.g. CdSe or CdTe)
High magnetic moment
and superparamagnetism
(e.g. iron oxide or ferrites)
These enhanced
properties can even be
combined by the use of
hybrid nanostructures
Magnetic field
core-shell decorated
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Using non-covalent interactions
high affinity ligand-receptor plataforms or hydrophobic association
The conjugation of nanoparticles with biomolecules (bionanosystems)
improves the biocompatibility and adds to the unique properties of
inorganic nanoparticles the ability of biomolecules for highly specific
binding by molecular recognition.
Building on the particle surface
Nanoparticles Biocompatibilization and Biofunctionalization
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The general objective is exploring the potential of
custom designed (bio)nanosystems for
Main areas of application @ CFUM
Environmental
applications such as
photoremediation and
energy
photoconversion
Enhanced detection
schemes based on
plasmonic resonances
Biosensing
and
controlled drug delivery
Fundamental studies of
energy transfer in
assembled systems of
quantum dots
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Time dependent fluorescence changes as a result of the interaction of functionalized
QDs with 0.5 mM NADH:
(1) before addition of NADH; (2) to (6) after successive time intervals of 3 min.
Angew. Chem. Int. Ed.
47 (2008) 1-6
Quantum dot based biosensor
Sensing of NADH by Nile-blue-functionalized CdSe/ZnS QDs
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Photodegradation kinetics:
Degussa TiO2 at 340 nm () and 405 nm ();
CdSe/TiO2 core/shell nanoparticles at 340 nm
() and 405 nm ().
The lines represent first order exponential
kinetics.
CdSe
TiO2
A. Fontes-Garcia et al., Nanoscale Res. Lett. 6 (2011) 426
FLIM images of four representative
superstructures obtained from CdSe/ZnS
(a, b) and CdTe (c, d) QDs capped with
thioglycolic acid, deposited on glass coated
with polylysine. The color scale ranges from
low (blue) to high lifetime values (red).C. Bernardo et al., J. Phys. Chem. C 118 (2014) 4982.
More details in poster “Energy transfer via
exciton transport in quantum dot based self-
assembled superstructures”, by C. Bernardo
Quantum dots @ CFUM
Photodegradation Energy transport
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CdTe/BSA/Dye bionanoconjugate
B. Raju et al., Dyes Pigments 110 (2014) 203
Quantum dots
Bionanoconjugates @ CFUM Research area evolution @ PT
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Quantum Dots
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Quantum Dots+ bio, + conjugat*, + functional*
0.28%
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Biomagnetic separations of
DNA, proteins, etc. Targeted drug delivery in vivo
Magnetic nanoparticles
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Magnetic
nanoparticles
cluster
covered by a
lipid bilayer
Magnetic
nanoparticles
Aqueous
media
Magnetoliposomes @ CFUMMnFe2O4 MNPs
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A.R.O. Rodrigues et al.,
Mat. Chem. Phys. 148
(2014) 978.
A.R.O. Rodrigues et al.,
Phys. Chem. Chem. Phys.
17 (2015) 18011.
A.R.O. Rodrigues et al.,
RSC Advances 6 (2016)
17302.
Research area evolution @ PT
Magnetoliposomes @ CFUM
Fusion of magnetoliposomes of Ni/SiO2 NPs
with biological membrane models
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Magnetic Nanoparticles
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Magnetic Nanoparticles+ bio, + conjugat*, + functional*
0.42%
Magnetic Nanoparticles
More details in poster “Magnetic liposome-based nanocarriers
for dual cancer therapy”, by A. R. O. Rodrigues
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J. Am. Chem. Soc. 125 (2003) 1643–1654
(a) shell = 0 nm (c) shell = 4.6nm (e) shell =12.5nm
Gold nanoparticles with silica shell
(size of Au core is 13.2 nm)
Metallic Surface Plasmon Enhanced Fluorescence
Plasmonic nanoparticles
J. Phys. Chem. B 105 (2001) 3441-3452Detection of a specific DNA sequence
Anal. Bioanal. Chem. 394 (2009) 47-59
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J. Am. Chem. Soc. 129 (2007) 6378
Surface Enhanced Raman Scattering
Plasmonic nanoparticles
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The dendrite-type objects emerged from a
homogeneous and highly transparent
Ag:TiO2 nanocomposite, via the
mechanism of diffusion-limited-aggregation
(DLA) of Ag atoms, during heat-treatment
at 500 °C.
Plasmonic nanoparticles
Research area evolution @ PTAg fractals on top of TiO2 matrix
@ CFUM
J. Borges et al., Phys. Status Solidi RRL (2016)
(DOI: 10.1002/pssr.201600131)
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Plasmonic Nanoparticles
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Plasmonic Nanoparticles+ bio, + conjugat*, + functional*
0.36%
More details in poster “Nanoplasmonic thin films for
LSPR-(Bio)sensing applications”, by J. Borges
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Thanks for your
attention
Paulo J. G. Coutinho
Departamento/Centro de Física
Escola de Ciências
Universidade do Minho
4 Julho 2016
Ciência 2016