application of triploidy to an emergent...
TRANSCRIPT
Application of Triploidy to an Emergent Oyster
Culture Industry on Florida’s West Coast:
Results of Health Assessment
Nick Brandimarte and Dr. Susan
Laramore
Aquatic Animal Health Lab
Florida Atlantic University HBOI
Ft. Pierce, FL
Application of Triploidy to an Emergent Oyster
Culture Industry on Florida’s West Coast
OBJECTIVE:
1) Assess health of diploid
(2N) and triploid (3N) oysters
- site
- gear
females
Funded by:2015-2018
3N
2N
Methods
• Trial 1: Plant July/August 2016 / Harvest March/April 2017
• Trial 2: Plant March/April 2017 / Harvest October/November 2017
VARIABLES MEASURED:
Variables Reporting• Shell Metrics
– Shell height
– Shell length
– Shell width
• Weight Metrics – Total weight
– Meat weight (wet)
– Shell weight
• Prevalence of Pests and Parasites– Boring Sponge (Cliona sp.)
– Mudblisters (P. websteri)
– Cestodes and trematodes
– Dermo (P. marinus)
• Physiological Condition
• Digestive Tubule Atrophy
3% 3%
10%
0%
27%
0%
32%
0%
13%
7%
0% 0%0%
10%
20%
30%
40%
Trial 1: Mudblisters (P. websteri)
Bottom Cage Bottom CageFloating Bag Adjustable LL Floating Bag Floating Bag
30%
66%
60%
40%
73%
41%
83%
80%
17%
0%
7%
0%0%
10%
20%
30%
40%
50%
60%
70%
80%
90%
100%
Bottom Cage Bottom CageFloating Bag Adjustable LL Floating Bag Floating Bag
Trial 2: Mudblisters (P. websteri)
Trial 1: Boring sponge (Cliona spp.)
30%
66%
0% 0% 0% 0%3%
15%
0%
10%
0% 0%0%
10%
20%
30%
40%
50%
60%
70%
Bottom Cage Bottom CageFloating Bag Adjustable LL Floating Bag Floating Bag
Trial 2: Boring sponge (Cliona spp.)
Bottom Cage Bottom CageFloating Bag Adjustable LL Floating Bag Floating Bag
0% 0% 0% 0%
10%
0%
47%43%
100% 100%
63%67%
0%
10%
20%
30%
40%
50%
60%
70%
80%
90%
100%
Trial 1: Prevalence of Dermo (P. marinus)
59%
41%
0% 0%
13%
40%
59%
41%
3%0% 0% 0%
0%
10%
20%
30%
40%
50%
60%
70%
Bottom Cage Bottom CageFloating Bag Adjustable LL Floating Bag Floating Bag
Trial 2: Prevalence of Dermo (P. marinus)
Bottom Cage Bottom CageFloating Bag Adjustable LL Floating Bag Floating Bag
0% 0%
17%
10% 10%
23%
60%
27%
50%
48%
0%
3% 3% 3%
0%
10%
20%
30%
40%
50%
60%
70%
Trial 1:Physiological Condition
Bottom Cage Bottom CageFloating Bag Adjustable LL Floating Bag Floating Bag
1.6
1.3
2.4
2.6
2.12
2.12.2
2.1
2.32.2
2.4
0
1
2
3
3
2
1
Trial 2:Physiological Condition
Bottom Cage Bottom CageFloating Bag Adjustable LL Floating Bag Floating Bag
1.9 1.92
2.4
2
2.2
1.9
2.2
2
2.3
2.1
2.3
0
1
2
3
3
2
1
Trial 1: Presence of food in the Gut and Sex Ratios
• A larger percentage of guts were full for oysters reared
using adjustable longline and floating bag gear
• Diploids and triploids had similar percentages food
present in the gut across sites
Presence of food in the gut
Sex ratios of diploids and triploids reared using on
and off bottom culture methods; red = females,
blue = males, grey = undifferentiated
Sex Ratios
A B
Conclusions
• Benefits derived from using triploids may be seen in the form of:
- improved digestive system health
- improved physiological condition for fall harvest
- increased size
- decreased prevalence and severity of mudworms
Take Home
- culture methods
- site to site variability (salinity
and temperature)
• Fall plant may
- reduce prevalence of mudblisters
- improve physiological condition
• The degree to which farmers can expect to benefit from using triploids oysters
is likely dependent on: 2N 3N
Acknowledgements
• All participating oyster growers
• Funding: NOAA National Sea Grant
• Graduate Committee
- Dr. John Scarpa, Dr. John Baldwin
and Dr. Paul Wills
• Support during sampling and processing
- Leslie Sturmer, Carter and Alfred
• Funding sources
- National Oceanic and Atmospheric
Administration and the Louisiana Sea Grant