application des isotopes stables en ecologie trophique et ... · application des isotopes stables...
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Application des isotopes stables en Ecologie
trophique et Ecotoxicologie
Gilles Lepoint, François Remy, Loïc Michel & Krishna Das
Laboratoire d’Océanologie
Université de Liège
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Stable isotopes in Trophic ecology and Ecotoxicology - Pessac -September 2014
Studies in the Oceanology Lab involving Stable isotopes (C,N, S):
•Delineation of marine trophic web
•Trophic ecology of aquatic animals (aquatic
invertebrates, fishes, birds, marines mammals)
•Relation between trophic ecology and ecotoxicology
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Stable isotopes in Trophic ecology and Ecotoxicology - Pessac -September 2014
Studies in the Oceanology Lab involving Stable isotopes (C,N, S):
•Delineation of marine trophic web
•Trophic ecology of aquatic animals (aquatic
invertebrates, fishes, birds, marines mammals)
•Relation between trophic ecology and ecotoxicology
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EA – IRMS
(Isoprime 100 – Vario MicroCube)
and
MS- GC- IRMS
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Stable isotopes in Trophic ecology and Ecotoxicology - Pessac -September 2014
"You are what you eat…plus a few per mille"
DeNiro & Epstein, 1978
MIXING Law:
"YOU ARE WHAT YOU EAT"
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Stable isotopes in Trophic ecology and Ecotoxicology - Pessac -September 2014
x« Plus few per mille »
Plus few per mille = trophic enrichment factor (TEF)
= trophic fractionation factor
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Stable isotopes in Trophic ecology and Ecotoxicology - Pessac -September 2014
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Stable isotopes in Trophic ecology and Ecotoxicology - Pessac -September 2014
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Our question:
Is it possible from isotopic
composition of an animal
to calculate the different
contribution of potential
food sources to its diet?
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Stable isotopes in Trophic ecology and
Ecotoxicology - Pessac - September 2014
...) ( cb aam cb fff
Mixing equation for n sources:
Complex mixing modelling
IsoSource (Philips et al., 2001) or SIAR (Parnell et al. 2010) or ….
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Stable isotopes in Trophic ecology and
Ecotoxicology - Pessac - September 2014
Case study 1: Trophic ecology of Gammarus aequicauda (amphipoda)
1 cmFrançois Remy ©
Gammarus aequicauda
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Seagrass meadow (Posidonia
oceanica)
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Exported
Dead Leaves
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Stable isotopes in Trophic ecology and Ecotoxicology - Pessac -September 2014
• More than 120 species
• Up to 6000 id.m-2
• Dominated by amphipods
• Prey for fishes
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Stable isotopes in Trophic ecology and Ecotoxicology - Pessac -September 2014
-35 -30 -25 -20 -15 -100
1
2
3
4
5
13
C: G.aequicauda: -16.4 08G.fucicola: -19.7 ± 0.5
d15
N: G.aequicauda: 3.0 ± 0.6G.fucicola: 2.2 ± 0.4
13
C (‰)
1
5N
(‰
)
Source: Lepoint et al. 2006
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Stable isotopes in Trophic ecology and Ecotoxicology -Pessac - September 2014
TEF All the same
Δ13C = + 1
Δ15N = + 3.4
SIAR Modelling
Litter Algae Epiphytes Amphipodes
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Stable isotopes in Trophic ecology and Ecotoxicology - Pessac -September 2014
-35 -30 -25 -20 -15 -100
1
2
3
4
5
13C: G.aequicauda: -16.4 08
G.fucicola: -19.7 0.5
15N: G.aequicauda: 3.0 0.6
G.fucicola: 2.2 0.4
13C (‰)
‰
Source Lepoint et al. 2006
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TEF Determination: Experimental design
3 different treatments :
• Freshwater amphipod powder
• Green algae powder
• Dead P. oceanica powder
• Different carbon and nitrogen isotopic compositions
• Different quality (C/N ratios)
• All potentially ingestible by G. aequicauda
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Amphipods Green algae Dead leaves
0,7 L
TEF Determination: Experimental design
• Controled conditions
• 96 ind / treatment (individual isotopic compositions
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δ13C = -24.86 + 8.62e-0,0591t
t1/2 = 11.72 days
TEF Calculations and C turnover
R² = 0,8019
P < 0.000001
Food source δ13C
Amphipod as food
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δ13C = -10.87 – 5.23e-0,0134t
t1/2 = 51.62 days
Far too long !
R² = 0,6923
P < 0.0001
Food source δ13C
Posidonia litter as
food
• High mortality
• Very slow
assimilation
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Food source δ13C
Green Algae as food:
Even worst (algae toxicity)
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No significant change of isotopic composition
No turnover rates calculation
Apparently…
Food source δ15N
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Trophic Enrichment Factors : TEFs or Δ
Treatment 1 typical of predator
Treatments 2 & 3 typical of primary detritic-feeder
1 2 3Amphipod treatment Algae treatment Litter treatment
Δ13
C (‰) 0.81 ± 0.39 / 1.19 ± 0.13
Δ15
N (‰) 2.91 ± 0.56 0.53 ± 0.44 0.96 ± 0.42
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Stable isotopes in Trophic ecology and Ecotoxicology - Pessac -September 2014
SIAR Modeling : the return
TEF: food source
specific
(experimentally
determined by
Michel for epiphytes
and by Remy for
litter, sciaphilous
algae and animal
diets)
Litter Algae Epiphytes Amphipodes
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Stable isotopes in Trophic ecology and Ecotoxicology - Pessac -September 2014
* Bulk IRMS does not discriminate different
component of epiphytic community
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Stable isotopes in Trophic ecology and Ecotoxicology - Pessac -September 2014
• Discriminate different component of
epiphytic community using GC –IRMS
Measurement of delta 13C on fatty acids
Next Elementar Seminar
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Stable isotopes in Trophic ecology and Ecotoxicology - Pessac -September 2014
CASE STUDY 2: TO ELUCIDATE CONTAMINATION PATHWAY OF AN ORGANOCHLORINE PESTICIDE
Organochlorine pollution in tropical rivers
(Guadeloupe): Role of ecological factors in
food web bioaccumulation
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Applications of Biomarkers in Aquatic food web studies - Liège -30 January 2013
•Heavy contamination by organochlorine
pesticides (Banana culture)
•What is the general structure of the trophic web?
•Is there a relation between trophic level and pollutant
contamination
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Figure 2: Example of crustacean species found the river
Pérou fauna (photos: Nicolas Marichal)
Figure 1: Pérou River sampling site (Guadeloupe)
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Source: Coat et al. (2009) Freshwater Biology 54, pp. 1028-1041 ,
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Source: Coat et al. (2011), Environmental Pollution 159: 1692-1701
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Stable isotopes in Trophic ecology and Ecotoxicology - Pessac -September 2014
CASE STUDY 3: Brominated and phosphorus flame retardants in White-tailed Eagle
Haliaeetus albicilla nestlings: Bioaccumulation and associations with
dietary proxies (δ13C, δ15N and δ34S)
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Stable isotopes in Trophic ecology and Ecotoxicology - Pessac -September 2014 source: Eulaers et al., 2014
C, N, S stable isotopes measurements in feathers
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Thank you for your attention