ap chapter 6 notes 2
TRANSCRIPT
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Chapter 6 notes
A Tour of the Cell
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Concept 6.1Light Microscope: visible light is passed
through a specimen and then through
glass lenses
- magnification
- resolving power
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Concept 6.1Electron Microscope: focuses beams of
electrons through the specimen or onto
its surface.- transmission electron microscope(TEM): used to study the internalultrastructure of cells
- Scanning electron microscope (SEM):used for detailed study of the surface
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Concept 6.1Cell Fractionation: take cells apart and
study the fcn. of organelles
- use of ultracentrifuges
-fig. 7.3
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Concept 6.2All cells have several basic features:
- have a plasma membrane
- cytsol: semifluid substance thatcontains organelles
- chromosomes/DNA
- ribosomes
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Concept 6.2Prokaryotic cell vs. Eukaryotic cell
- nucleiod vs. nucleus
- eukaryotes are 10X bigger
Larger organisms do not have larger cells,just more cells
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Concept 6.2 PiliNucleoid
RibosomesPlasma membrane
Cell wallCapsule
Flagella
Bacterialchromosome
(a) A typical rod-shapedbacterium (b) A thin section
through thebacteriumBaci l lus
coagulans
(TEM)
0.5
m
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Concept 6.2Internal membranes compartmentalize
the fcns. of a eukaryotic cell
- membranes consist of a double layerof phospholipids and other lipids;attached to the surface are proteins
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Concept 6.2 TEM of a plasmamembrane(a)
(b) Structure of the plasma membrane
Outside of cell
Inside of
cell 0.1 m
HydrophilicregionHydrophobic
regionHydrophilic
regionPhospholipid Proteins
Carbohydrate side chain
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Concept 6.2
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Concept 6.3The nucleus is a double membrane
- inside is lined by the nuclear lamina(protein filaments that help the nucleusmaintain shape)
- DNA is organized w/ proteins into
chromatin
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Concept 6.3As a cell prepares to divide, the chromatin
fibers coil up into chromosomes
Nucleolus: ribosomal RNA is synthesizedw/ proteins into ribosomal subunits
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Concept 6.3Nucleolus Nucleus
Rough
ER
Nuclear lamina(TEM)
Close-up ofnuclearenvelope
1 mRibosome
Porecomplex
Nuclearpore
Outer membraneInner membraneNuclear envelope:
Chromatin
Surface of
nuclearenvelope
Pore complexes(TEM)
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Concept 6.3Ribosomes: organelles that carry out
protein synthesis
- cells that have high rates of proteinsynthesis have several ribosomes
- free ribosomes:
- suspended in the cytosol- fcn. within the cell
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Concept 6.3- bound ribosomes:
- attached to the outside of the ER
or nuclear envelope
- protein insertion into membranes,packaging (lysosomes), or export
from cell (excretion)
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Concept 6.4Endomembrane system: made up of
many different membranes
Endoplasmic reticulum: manufacturesmembranes and performs many otherbiosynthetic functions
- accounts for more than half the totalmembrane in many eukaryotic cells
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Concept 6.4Smooth ER:
- surface lacks ribosomes
- synthesis of lipids, metabolism ofcarbohydrates, detoxification of drugsand poisons
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Concept 6.4Rough ER:
- specialized cells secrete proteins
produced by ribosomes attached torough ER
- ex. Secretion of insulin in pancreas
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Concept 6.4The Golgi apparatus finishes, sorts, and
ships cell products
- after leaving the ER, many transportvesicles travel to the Golgi
- consists of flattened sacs, called
cisternae
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Concept 6.4A Golgi stack has distinct polarity
- cisface: receiving side, gets material
from the ER
- transface: shipping side, gives riseto vesicles that will travel to other sites
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Concept 6.4
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Concept 6.4Lysosomes are digestive compartments
- membrane-bound sac of enzymes that
digest macromolecules
- different enzymes digest differentmolecules
Many protists use lysosomes to digestfood through phagocytosis
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Concept 6.4
Vacuoles have diverse fcns. in cellmaintenance
- food vacuole: formed by phagocytosis- contractile vacuole: pump excesswater out of a cell
- central vacuole: hold reserves oforganic compounds, disposal site forbyproducts, contains pigments
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Concept 6.4
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Concept 6.5
Mitochondria and chloroplasts areorganelles that convert energy that cells
can use for workMembrane proteins are not made by the
rough ER, but by free ribosomes
Organelles also contain DNA
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Concept 6.5
Mitochondria: site of cellular respiration
- found in most all eukaryotic cells;
number of mitochondria is correlatedwith the cells metabolic activity
- enclosed in a double membrane
- outer layer is smooth, innercontains infoldings called cristae(gives a large surface area)
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Concept 6.5
- mitochondrial matrix is enclosed inthe inner membrane
- contains many enzymes,mitochondrial DNA, and
ribosomes
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Concept 6.5
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Concept 6.5
Chloroplasts are specialized plantorganelles (contain chlorophyll)
- thylokoids: membranous system offlattened sacs (each stack is a grana)
- stroma: fluid outside the thylokoids
- contains chloroplast DNA andribosomes
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Concept 6.5