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    Chapter 6 notes

    A Tour of the Cell

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    Concept 6.1Light Microscope: visible light is passed

    through a specimen and then through

    glass lenses

    - magnification

    - resolving power

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    Concept 6.1Electron Microscope: focuses beams of

    electrons through the specimen or onto

    its surface.- transmission electron microscope(TEM): used to study the internalultrastructure of cells

    - Scanning electron microscope (SEM):used for detailed study of the surface

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    Concept 6.1Cell Fractionation: take cells apart and

    study the fcn. of organelles

    - use of ultracentrifuges

    -fig. 7.3

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    Concept 6.2All cells have several basic features:

    - have a plasma membrane

    - cytsol: semifluid substance thatcontains organelles

    - chromosomes/DNA

    - ribosomes

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    Concept 6.2Prokaryotic cell vs. Eukaryotic cell

    - nucleiod vs. nucleus

    - eukaryotes are 10X bigger

    Larger organisms do not have larger cells,just more cells

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    Concept 6.2 PiliNucleoid

    RibosomesPlasma membrane

    Cell wallCapsule

    Flagella

    Bacterialchromosome

    (a) A typical rod-shapedbacterium (b) A thin section

    through thebacteriumBaci l lus

    coagulans

    (TEM)

    0.5

    m

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    Concept 6.2Internal membranes compartmentalize

    the fcns. of a eukaryotic cell

    - membranes consist of a double layerof phospholipids and other lipids;attached to the surface are proteins

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    Concept 6.2 TEM of a plasmamembrane(a)

    (b) Structure of the plasma membrane

    Outside of cell

    Inside of

    cell 0.1 m

    HydrophilicregionHydrophobic

    regionHydrophilic

    regionPhospholipid Proteins

    Carbohydrate side chain

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    Concept 6.2

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    Concept 6.3The nucleus is a double membrane

    - inside is lined by the nuclear lamina(protein filaments that help the nucleusmaintain shape)

    - DNA is organized w/ proteins into

    chromatin

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    Concept 6.3As a cell prepares to divide, the chromatin

    fibers coil up into chromosomes

    Nucleolus: ribosomal RNA is synthesizedw/ proteins into ribosomal subunits

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    Concept 6.3Nucleolus Nucleus

    Rough

    ER

    Nuclear lamina(TEM)

    Close-up ofnuclearenvelope

    1 mRibosome

    Porecomplex

    Nuclearpore

    Outer membraneInner membraneNuclear envelope:

    Chromatin

    Surface of

    nuclearenvelope

    Pore complexes(TEM)

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    Concept 6.3Ribosomes: organelles that carry out

    protein synthesis

    - cells that have high rates of proteinsynthesis have several ribosomes

    - free ribosomes:

    - suspended in the cytosol- fcn. within the cell

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    Concept 6.3- bound ribosomes:

    - attached to the outside of the ER

    or nuclear envelope

    - protein insertion into membranes,packaging (lysosomes), or export

    from cell (excretion)

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    Concept 6.4Endomembrane system: made up of

    many different membranes

    Endoplasmic reticulum: manufacturesmembranes and performs many otherbiosynthetic functions

    - accounts for more than half the totalmembrane in many eukaryotic cells

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    Concept 6.4Smooth ER:

    - surface lacks ribosomes

    - synthesis of lipids, metabolism ofcarbohydrates, detoxification of drugsand poisons

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    Concept 6.4Rough ER:

    - specialized cells secrete proteins

    produced by ribosomes attached torough ER

    - ex. Secretion of insulin in pancreas

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    Concept 6.4The Golgi apparatus finishes, sorts, and

    ships cell products

    - after leaving the ER, many transportvesicles travel to the Golgi

    - consists of flattened sacs, called

    cisternae

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    Concept 6.4A Golgi stack has distinct polarity

    - cisface: receiving side, gets material

    from the ER

    - transface: shipping side, gives riseto vesicles that will travel to other sites

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    Concept 6.4

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    Concept 6.4Lysosomes are digestive compartments

    - membrane-bound sac of enzymes that

    digest macromolecules

    - different enzymes digest differentmolecules

    Many protists use lysosomes to digestfood through phagocytosis

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    Concept 6.4

    Vacuoles have diverse fcns. in cellmaintenance

    - food vacuole: formed by phagocytosis- contractile vacuole: pump excesswater out of a cell

    - central vacuole: hold reserves oforganic compounds, disposal site forbyproducts, contains pigments

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    Concept 6.4

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    Concept 6.5

    Mitochondria and chloroplasts areorganelles that convert energy that cells

    can use for workMembrane proteins are not made by the

    rough ER, but by free ribosomes

    Organelles also contain DNA

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    Concept 6.5

    Mitochondria: site of cellular respiration

    - found in most all eukaryotic cells;

    number of mitochondria is correlatedwith the cells metabolic activity

    - enclosed in a double membrane

    - outer layer is smooth, innercontains infoldings called cristae(gives a large surface area)

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    Concept 6.5

    - mitochondrial matrix is enclosed inthe inner membrane

    - contains many enzymes,mitochondrial DNA, and

    ribosomes

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    Concept 6.5

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    Concept 6.5

    Chloroplasts are specialized plantorganelles (contain chlorophyll)

    - thylokoids: membranous system offlattened sacs (each stack is a grana)

    - stroma: fluid outside the thylokoids

    - contains chloroplast DNA andribosomes

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    Concept 6.5