antioxidant responses during fusarium infection: the sampling and the first results gallé Ágnes
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Antioxidant responses during fusarium infection: the sampling and the first results
Gallé Ágnes
Fusarium head blight (FHB) is an important disease of wheat and has worldwide distribution, which results in yield loss and mycotoxin contamination of grain when the disease is caused by a mycotoxin-producing Fusarium species.
Fusarium graminearum and F. culmorum are the Fusarium species that most commonly cause FHB of wheat and both can produce trichothecene mycotoxins, predominantly deoxynivalenol (DON, also known as vomitoxin) (Parry et al. 1995).
The fungus produces trichothecene mycotoxins, such as deoxynivalenol (DON).
Effect: • livestock feed refusal• depression of the immune system• nausea and vomiting in humans (Hall and Van Sanford, 2003)
Resistance to FHB is very complex and is considered to be quantitatively governed by numerous resistance
genes located on different chromosomes.
At least 18 of the 21 wheat chromosomes have been reported to be associated with resistance.
The components of the defense reactions have been extensively investigated with both protein and
transcript profiling.
Some gene families which were induced during Fusarium infection (microarray): Saber et al 2007
wheat spikelets infected with F. graminearum
Some gene families which were induced during Fusarium infection (differential display RT-PCR):Ansari et al 2007
• Proteins with an antioxidant function: superoxide dismutase, dehydroascorbate reductase, and glutathione S-transferases (GSTs)
• A 14-3-3 protein homolog was also upregulated
• PR-2 protein (β-1, 3 glucanase) was upregulated in FHB-infected spikes
Expression of proteins with highest similarity to dehydroascorbate reductase and TaGSTF5 (a glutathione S-transferase) differed following FHB infection
in susceptible and resistant cultivars.
wheat spikelets infected with F. graminearum
Some protein families (examples) which were induced during Fusarium infection (2-dimensional displays of proteins): Zhou et al 2005
SOD, KAT, GR, POD and GST activity determination
GK Mini Manó/Nobeokabozu genetic mapping population
(Prof. Mesterházy Ákos )
Nobeokabozu: FHB resistant lineA recombinant line from China: ‘WSY’, Wangshubai/Sumai3/Yangangfanzhou
GK Mini ManóArthur 71 / Sava // Rusalka / NS 171.2 /3/ F 30.74Resistant line to e.g. leaf rust
Plant material
Gathering wheat ears and flag leaves 17. 06. 2010. Kecskéstelep,
Cereal Research Nonprofit Kht.GK Mini Manó/Nobeokabozu wheat population
(Prof. Mesterházy Ákos )
SOD activity
0
2
4
6
8
10
12
14
16
18
20
↓Mn resistent ↑ Mn resistent ↓ Mn sensitive ↑ Mn sensitive
SO
D a
ctiv
ity (
U m
g-1
tota
l pro
tein
)
KAT activity
05
10152025303540
↓Mn resistent ↑ Mn resistent ↓ Mn sensitive ↑ Mn sensitive
KA
T a
ctiv
ity (
U m
g-1
tota
l pr
otei
n)
GR activity
0100
200300
400500
600700
800900
1000
↓Mn resistent ↑ Mn resistent ↓ Mn sensitive ↑ Mn sensitive
GR
act
ivity
(U
mg
-1 to
tal p
rote
in)
POD activity
0,000
2,000
4,000
6,000
8,000
10,000
12,000
↓Mn resistent ↑ Mn resistent ↓ Mn sensitive ↑ Mn sensitivePO
D a
ctiv
ity (
U m
g-1
tota
l pro
tein
)
Guaiacol peroxidase activity (Class III peroxidase)
Thank you for your attention!