antioxidant properties and cytotoxicity of crude polysaccharides from
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8/3/2019 Antioxidant Properties and Cytotoxicity of Crude Polysaccharides From
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Antioxidant properties and cytotoxicity of crudepolysaccharides from Lentinus polychrousLv.
Chatchai Thetsrimuanga, Saranyu Khammuanga, Khajeelak Chiablaemb,
Chantragan Srisomsapb, Rakrudee Sarnthimaa, ,
Purchase
a Proteins and Enzymes Technology Research Unit and Center of Excellence forInnovation in Chemistry, Department of Chemistry, Faculty of Science, MahasarakhamUniversity, Maha Sarakham 44150, Thailand
b Laboratory of Biochemistry, Chulabhorn Research Institute, Bangkok 10210, Thailand
Received 14 June 2010; revised 22 February 2011; Accepted 16 March 2011. Available online
22 March 2011.
Abstract
Crude polysaccharides of mature fresh and dried fruit bodies and dried mycelia of Lentinus
polychrousLv. were evaluated for their antioxidant properties and cancer cell line cytotoxicity.
The crude polysaccharides were in yields of 39.097.5 mg/g dry weight of sample. Trolox
equivalent values in scavenging abilities of those crude polysaccharides against both 2,2-azino-
bis-(3-ethylbenzthiazoline-6-sulphonic acid (ABTS +) radical and 1,1-diphenyl-2-picrylhydrazyl
(DPPH ) radicals were in the range of 53.4131 and 6.438.8 mol trolox/g dwt of extract,
respectively, whereas values of reducing power were in the range 27.654.9 mol trolox/g dwt
of extract. Scavenging ability and reducing power of these crude polysaccharide extracts were
in the descending order of mycelia > dried fruit bodies > fresh fruit bodies. In vitrocytotoxicity,
determined with the crude polysaccharides of fresh and dried fruit bodies of the fungus at a
concentration of 1 mg/ml, by the [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide]
(MTT) method, showed cytotoxic effects of about 38% and 45%, respectively, against the
human breast adenocarcinoma cell line (MCF-7). This mushroom might be a good source of
bioactive compounds for cancer prevention.
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8/3/2019 Antioxidant Properties and Cytotoxicity of Crude Polysaccharides From
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Highlights
We report antioxidant and anti-cancer of polysaccharides of Lentinus polychrousLv.
Antioxidant activity of mycelia > dried fruit bodies > fresh fruit bodies. Crude polysaccharides
fraction consist of proteins and phenol compounds. Fruit bodies fraction show fair cytotoxic
effect towards MCF-7, SK-Hep1, and A549. The mushroom might serve as a possible
protective agents source for human.
Keywords: Antioxidant; Cytotoxicity assay; Lentinus polychrousLv.; Polysaccharide;
Reducing power
Article OutlineFood ChemistryVolume 128, Issue 3, 1 October 2011, Pages 634-639
doi:10.1016/j.foodchem.2011.03.077|How to Cite or Link Using DOI Cited By in Scopus (0)
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Sulfation of the extracellular polysaccharide produced by theedible mushroom Pleurotus sajor-cajualters its antioxidant,anticoagulant and antiproliferative propertiesin vitro
Cinthia B.S. Tellesa, Diego A. Sabry
a, Jailma Almeida-Lima
a, Mariana S.S.P. Costa
a, Raniere F. Melo-Silveira
a,
Edvaldo S. Trindadeb, Guilherme L. Sassakic, Elisabeth Wisbeckd, Sandra A. Furland, Edda L. Leitea, Hugo
A.O. Rochaa, ,
Purchase
a Laboratrio de Biotecnologia de Polmeros Naturais-BIOPOL, Departamento deBioqumica, Universidade Federal do Rio Grande do Norte - UFRN, Natal, RN, Brazil
b Departamento de Biologia Celular, Universidade Federal do Paran UFPR, Curitiba,PR, Brazil
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8/3/2019 Antioxidant Properties and Cytotoxicity of Crude Polysaccharides From
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c Departamento de Bioqumica, Universidade Federal do Paran UFPR, Curitiba, PR,Brazil
d Universidade da Regio de JoinvilleUNIVILLE, Joinville, SC, Brazil
Received 25 October 2010; revised 21 February 2011; Accepted 22 February 2011. Available online 1 March 2011.
Abstract
An extracellular polysaccharide (PN) composed of mannose:3-O-methyl-galactose:galactose:glucose
(44.9:16.3:19.8:19) was obtained from Pleurotus sajor-caju. This polymer showed specific antioxidant activities such
as total antioxidant capacity, superoxide radical scavenging, reducing power and ferric chelating, bu t it did not
possess anticoagulant or antiproliferative activities. Through chemical modification of PN, the hydroxyl radical
scavenging and reducing power activities of PN increased. A derived sulfated polysaccharide of PN, with a DS of
0.17 and named PS, was obtained using a sulfuric acid/n-propanol method. The sulfation was confirmed by
electrophoresis, NMR and FT-IR. In addition, PS prolonged the time of coagulation in a concentration-dependent
manner in aPTT test. The antiproliferative effect against HeLa cells was time dependent; after 72 h, the inhibition rate
of PS (1.0 mg/mL) was approximately 60%. PS demonstrated promising antioxidant, antiproliferative and
anticoagulant properties and has been selected for further studies.
Keywords: Mannogalactoglucan; Sulfated polysaccharide; Biological activities; HeLa cells
Article Outline
Abstract- selectedArticleFigures/TablesReferences
Carbohydrate PolymersVolume 85, Issue 3, 1 June 2011, Pages 514-521
doi:10.1016/j.carbpol.2011.02.038|How to Cite or Link Using DOI Cited By in Scopus (0)
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