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Table 1 Primer sets designed for this assays

Assay target Primer name sequence (53) References1!rR"A genes #$5%A&A&TT T&AT''T&&'T'A&%3

R$5%TA'&&'TA''TT&TTA'&A'TT%3

iao et al *++,

Aerolysin gene A-A#

A-AR

5%&A&AA&&T&A''A''AA&AA'A%3

5%&&TA&&A&ATT&&'TT&TA&A&%3

This study

Haemolysin

gene

.em #

.em R

5%'A''&&TTT''&&A&AAATA%3

5%'''A&&'&TAT'''AAATAA%3

This study

Lipase gene /i0 #

/P R

5%'&A''TATA''T&TA&&'TT'T%3

5%T&'&&&A&&AATA&&ATT&A%3

This study

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Table *2iochemical characteristics of the isolates

Test items .4A3 .4A* .4A1

&ram staining

4otility

6idase

'atalase

&lucose

!ucrose/actose

Arabinose

4annitol

Aesculin

4ethyl red

-%P reaction

'itrate 7tili8ation

9sculine .ydrolysis

ndole Production

.*! Production

!atrch hydrolysis

Aeromonas media

%

:

:

%

%

::

:

:

%

:

%

:

%

:

%

:

:

%

:

:

%

%

::

:

:

:

:

:

%

%

:

:

:

:

%

:

:

%

%

::

:

:

:

:

:

%

%

:

:

:

:

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Table 3 -alidation of real%time P'R assays usingAeromonas veronii

Strain

rolysin H molysin Lipas

WHMA1 35.21 32.76 32.92

WHMA2 34.93 30.93 31.73

WHMA3 34.25 32.10 31.26

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Table ; !usce0tibility ofA.veroniito *+ one (mm)

!usce0tibility

Amo6ilin

#lorfenicol

/e