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KRS KKNI is under development

STATE UNIVERSITY OF MALANGDEPARTMENT OF BIOLOGY

SYLLABI

Course Name Biomolecular Analysis Techniques

For Animal Studies

Code

Lecturer Name Dra. Dwi Listyorini Aljabari, M.Si, D.Sc

Semester 2th. Y’ 2014/2015 Credit/Hours 2/4

Student to attend 15 students/offering Classroom Bio 316

Prerequisites Biochemistry, Cell Biology, Genetics I, Genetics II

Compulsory—Elective—Others: Compulsory for Biology Program

Common—Fundamental—Special—Others: Special

Course Objectives 1. To understand the basic works in molecular techniques.

2. To be able to perform certain basic techniques in molecular

works in a right way.

3. To be able to apply certain molecular techniques in a

proposed research project.

4. To be able to take a part in molecular laboratory jobs.

Course description Introduction; mind setting; mental setting; basic works in

molecular techniques; gene anatomy; gene expression and

regulation; gene manipulation; molecular cloning; Recombinant

DNA; molecular techniques including: special equipment/devises

sterilization, micro pipetting, microtransfer, micro centrifuging,

temperature handling, temperature-sensitive reagents handling,

harmful reagent handling, whole DNA isolation, enzymatic DNA

digest, electrophoresis, gene to vector insertion, bacterial

transformation, molecular identification, PCR, DNA sequencing,

RNA probe synthesis. Bioinformatics: Genetics analysis for

identification, Biosystematics, Phyllogenetics and Regulation.

Listyorini D. Aljabari, Mol.Tech. 2014. 1 of 4


Compulsory Textbooks

Brown, T. A. 2001. Genome. 2nd. Ed. NY: John Wiley & Sons, Inc.

Freifelder, D. 1985. Essential of Molecular Biology. Boston: Jones and Barlett Pub. Inc.

Listyorini, D., Winaris, N., & Kartikasari, N. 2009. Molecular Handbook: A Practical Approach. Unpublished.

Sambrook, J., Fritsch, E.F. & Maniatis, T........ Molecular Cloning: A Laboratory Manual. NY: Cold Spring Harbor laboratory Press.

Stern, C. In Situ Hybridization.

Watson, J.D., Tooze, J. & Kurtz, D.T. 1983. Recombinant DNA: A Short Course. NY: W.H. Freeman and Co.

Listyorini, D., Winaris, N., Kartikasari, N., Prananingrum, P., & Anggorowati, D. 2012. Teknik Analisis Biologi Molekular. Ed. 2. Unpublished.

Fatchiyah, dkk. 2012. Teknik Analisis Biologi Molekular

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Suggested Reading

Recent research articles in the field of Cell Biology, Genetics, Development, etc. published in the international journals.

Protocols from Biomolecular products.

Teaching Material/ Aids

LCD projector & Computer; Laboratory equipments.

Teaching Method

Theories for maximum 8 meeting

Lab works for maximum 8 meeting

Evaluation Method

Paper test

Lab work

Individual report on lab work

Research Proposal

Request to Student (controlled exercise, experimental investigation, project, report)

To study and resume certain research reports published in scientific international journals.

To attend 100% lab works (practice).

To present an individual report 24 hours after a lab work has been completed.

To submit a research proposal in the end on semester

Listyorini D. Aljabari,. Mol.Tech. 2014. 2 of 4

KRS KKNI is under developmentSCHEDULE

Week Topic Activities

1 Introduction: Theory on Basic Techniques in Molecular Works

Mind & Mental Setting; Basic Works in Molecular Techniques; Basic Techniques in Molecular Works

2 Washing & Sterilization & Good Pipetting Practice

Lab Work: Equipment Washing & Sterilization; Work Place Sterilization Pipetting, Micropipetting, Microtransfer, Micro Centrifuging

3 Theory on Gene Anatomy & Regulation

Discussion: Gene Anatomy; Gene Expression & Regulation

4 Simple DNA Isolation Lab Work: Simple DNA Isolation (optional)

5 Molecular Genetics Techniques for Taxonomic and Regulation Studies

Discussion: DNA Isolation; Library DNA; DNA storage and Management; Gene Transfection, CISH; WISH; FISH; IHC; Blottings; Tagging; Finger printing

6 Lab work: Plant Total DNA isolation

7 Yield Examination & Trouble Shooter

Discussion: DNA Quantitazion; Qualitative Yield Examination, Electrophoresis: Preparation, Reading and Presentation

8 Lab Work: DNA Quantization, Electrophoresis Check

9 Molecular Cloning & Recombinant DNA

Discussion: Recombinant DNA, Bacterial Transformation;

10 Lab Work: DNA Cutting, Electrophoresis Check, DNA Ligation into Plasmid, Insert Check, Bacterial Transformation

11 Gene Isolation/Amplification Discussion: Primer Designing Polymerize Chain Reaction (PCR)

12 Lab Work: PCR and Sequencing

13 Gene Manipulation and Genetic Analysis

Discussion: Gene Manipulation and Mutation, Bioinformatics, Gene Bank, Phyllogenetic for Taxonomy and Evolution



Study, DNA Barcode

14 Lab Work: Sequence Examination, Consensus Sequence, Open Reading Frame (ORF), Genes Alignment, Phyllogenetic Tree Reconstruction, Genetic Distance Calculation and Analysis

15 Theory and Lab Work exam Examination

16 Research Project Completion Final Discussion on Proposed Research Project


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