analysis of tween based micro emulsion in the presence

8/8/2019 Analysis of Tween Based Micro Emulsion in the Presence

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` rifampicin has low solubility and bioavailability inboth fixed and single dose formulation

` factors- pH, absorption and metabolismcontributes to low bioavailability; hencetherapeutic activity affected


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` improves solubility and bioavailability of rifampicin

` protect against enzymatic hydrolysis

` enhances absorption due to surfactant inducepermeability and increase mobility

`

SMEDDS (self-microemulsifying drug deliverysystem) contains oil, & increases solubility,dissolution rate and bioavailability of drug


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` have different structures- (o/w, w/o)

` this helps in releasing the drug

` release of drug rapid for hydrophilic drug (o/w)

` diffusion difficult when in w/o trapped in water droplet

` dissolution studies- rifampicin controlled release isexpected from o/w emulsion droplet


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` MATERIALS

` Tween 80

` Rifampicin` Ethanol

` Oleic Acid

` Phosphate Buffer


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` A. Microemulsion Preparation

oleic acid and PB were mixed with Tween 80

ethanol was added

prepared in screw cap glass vials

observations


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` B. Drug incorporation inmicroemulsion


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` C. Microemulsion characterizationi) Optical Tr anspa re ncy

` homogeneity and optical isotropy

` examined by a cross polarizer and visual examination

` precipitation/phase separation


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ii) C e nt r ifugation

` 2 000rpm for 30 min


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iii) pH

` determined at room temperature.


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iv) S olubility S tudi e s

` solubility determinations

` filtration of material/dilution

` absorbance determined


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v) Oil/Buff er pa r tition co- e ffici e nt

` rifampicin dissolved in Oleic Acid

` centrifuged

`

content of rifampicin in aqueous layer assayed


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vi) Dissolution studi e s`

microemulsion containing Rifampicin filled inhard gelatin capsules.` introduced in 50ml PB.` stirred at constant speed of 2 50rpm.` 2 ml withdrawn at

0, 2 ,4,8,10,15, 2 0,30,40,60,80,100,130,160 minintervals.

` same amount of fresh PB added to maintaindissolution volume.

` filtered and drug [ ] determined.


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` study formulate microemulsion of oleicacid/phosphate buffer/tween 80/ethanol

` to investigate its potential as drug delivery system

for rifampicin` microemulsion subjected to several tests` results indicate

i) microemulsion remained

ii) ME-C optimum


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` solubility & partition studies- drug may reside atinterface of oil and aqueous phase

` paticle size analysis- w/o microemusion dropletchanges into o/w emlsion type

` drug enters the pallisade layer situated on the

inner side of droplet- resulting in controlled releaseof drug


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` slow release of rifampicin from formulation

` advantage- can also be used with high drug

concentrations


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` considering the physico- chemical andspectroscopic analysis of microemulsionformulation of rifampicin

` can be used as oral delivery system for rx of TB

` thus, microemulsion systems help in increasing

solubility of highly hydrophobic drug


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` perform different dissolution methods, eg.rotating basket

` patient compliance reduced microemulsiontaken orally & accurate dose cannot bedetermined

` further in vivo studies need to be done todetermine product acceptability

` change in variables- pH & degree of solubilization

` change in temperature- leads to drug optimum

analysis of tween based micro emulsion in the presence

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