Analysis of Coenzyme Q 10

By Taylor SineathBy Taylor Sineath

6-1-016-1-01

School of Pharmacy:

School of Pharmacy:School of

Pharmacy:

School of Pharmacy:

Purpose

To test the qualities of Coenzyme Q10 dietary To test the qualities of Coenzyme Q10 dietary supplements using analytical methods.supplements using analytical methods.

What is Coenzyme Q 10?

Naturally occurring compound found in Naturally occurring compound found in organisms. Used for a variety of indications organisms. Used for a variety of indications notably cardiovascular diseases. notably cardiovascular diseases.

Forms/Dosage

GNC/FoodLion both 10 mg capsulesGNC/FoodLion both 10 mg capsules GNC is in a capsule formGNC is in a capsule form FoodLion is in soft gel formFoodLion is in soft gel form

Testing Methods

Weight variationWeight variation DisintegrationDisintegration HPLC AssayHPLC Assay

Weight variation

Tests the variation in weight of capsulesTests the variation in weight of capsules Tests the deviation from the averageTests the deviation from the average Tests the net fill of soft gel (FoodLion)Tests the net fill of soft gel (FoodLion) Tests the weight of the capsules (GNC)Tests the weight of the capsules (GNC)

Disintegration

Tests the solubility in an environment Tests the solubility in an environment supposed to imitate that of the stomachsupposed to imitate that of the stomach

Used 6 capsules in sodium acetate buffer Used 6 capsules in sodium acetate buffer 3737ooC C ++/- 2/- 2ooC C The limit was 45min The limit was 45min

HPLC Assay

Determine concentration of analyte within a Determine concentration of analyte within a samplesample

Measures it by light absorptionMeasures it by light absorption Analyzed 3 capsules per lotAnalyzed 3 capsules per lot

HPLC Setup

Used a wavelength of 275nmUsed a wavelength of 275nm Flow Rate 1ml/minFlow Rate 1ml/min Column 150mm x 4.6mm Altima C18 3Column 150mm x 4.6mm Altima C18 3 Mobile Phase (25:75) Hexanes:MethanolMobile Phase (25:75) Hexanes:Methanol

Injection volume of 20 ulInjection volume of 20 ul

Sample Chromatogram

Sample Preparation – Soft gels

Cut open the shell using forceps and a Cut open the shell using forceps and a scalpel over a funnel in a 100ml volumetric scalpel over a funnel in a 100ml volumetric flask flask

Wash the funnel,forceps and the scalpel Wash the funnel,forceps and the scalpel into the 100ml flask with 20ml of hexanes into the 100ml flask with 20ml of hexanes

Sonicate for 5 min and diluted to volume Sonicate for 5 min and diluted to volume with methanolwith methanol

Then sonicate again for 5 minThen sonicate again for 5 min

Sample Prep. (cont.)

Pipetted 2ml of the sample into a 50ml Pipetted 2ml of the sample into a 50ml volumetric flask and diluted with methanolvolumetric flask and diluted with methanol

Filled a HPLC vial Filled a HPLC vial 11//22 to to 33//44 full full

Data from FoodLion Weight Variation

lot# 8HL701 9DNO572 9GN0749Ave (g) 0.1617 0.1600 0.1678%rsd 3.38 1.54 3.43

Data From GNC Weight Variation

Lot# 88306 82945 82040Ave(g) 0.2350 0.2344 0.2320% RSD 4.43 2.88 3.21

Disintegration of FoodLion Brand

Lot# 8HL701 9DNO572 9GNO749Min time 9min 50sec 5min 30sec 7min 20secMax time 10min 10sec 10min 05sec 9min 45sec

Disintegration of GNC Brand

lot# 88306 82945 82040Min time 2min 55sec 3min 45sec 2min 45secMax time 7min 00sec 8min 35sec 4min 25sec

Assay of FoodLion Soft gels

Lot #Lot # 9DN05729DN0572 8HL7018HL701 96N074996N0749

% found% found 106.2106.2 104.7104.7 85.085.0

% +/- % +/- 24.5824.58 6.36.3 14.514.5

mg/capmg/cap 10.610.6 10.510.5 8.58.5

mg/cap+/-mg/cap+/- 2.52.5 0.60.6 1.51.5

Assay of GNC capsules

Lot #Lot # 8294582945 8204082040 8830688306

% found% found 122.3122.3 52.052.0 107.2107.2

% +/- % +/- 10.510.5 58.958.9 4.34.3

mg/capmg/cap 12.212.2 5.25.2 10.710.7

mg/cap+/-mg/cap+/- 1.11.1 5.95.9 0.40.4

Conclusions

Weight variations within brands consistentWeight variations within brands consistent Disintegrations within brands consistentDisintegrations within brands consistent Assay would indicate variability within the lot and Assay would indicate variability within the lot and

from lot to lotfrom lot to lot Preliminary data suggests that FoodLion would be Preliminary data suggests that FoodLion would be

a better choicea better choice Costs less $10 vs. $17Costs less $10 vs. $17 100 capsules vs. 90 capsule in bottle100 capsules vs. 90 capsule in bottle Less variabilityLess variability