Analysis of bone ash

2015

Submitted by- Srishti Chauhan

Class xii b

Submitted to- Mrs. Kiran Pareek

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Acknowledgement

I sincerely thank my chem teacher Mrs. Kiran Pareek, for the help and guidance provided to me in completing the project in chemistry. I also thank the principal for permitting the use of the resources of the school for completing the project.

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Certificate

Name of princi

Name of grade teacher

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Aim

Analysis of bone ash

Index

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S.No. contents1 Intro2 Requirements3 Theme4 Chem. Eqn5 Procedure6 Observant table7 Conclusion8 Precaution9 bibliography

Intro

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Requirements

Rib Bone 2 PiecesBeaker 150 mlTest Tube 7 nosEvaporating Dish 1 noRing Stand 1 noBunsen Burner 1 noTest Tube Holder 2 nosFilter Paper -PH Paper -Dil. Nitric Acid 200 ml1% Ammonium Hydroxide 100 ml1% Silver Nitrate 25 ml1% Ammonium Chloride 50 mlAcetic Acid 100 ml1% Potassium Thiocyanate 25 mlDistilled Water As Reqd

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Theme (significance of pro)

Deals with analysis of bone ash Provides all basic ideas about concentration of various saltspresent Provides information about ion exchange reaction

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chem. Eqn

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Procedure

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Observation table

experiment observationA strip of bone was burnt in evaporating dish

Yellowish white precipitate was obtained

2 gms of bone ash was weighed To it dilute nitric acid was added

the ash sparingly dissolved

It was diluted with water the ash was completely dissolvedThe above solution was filtered and the residue (left on the filter paper) was discarded

The pH was made to 8.6Ammonium hydroxide was added to the filtrate (left on the beaker)

Whitish brown precipitate of Magnesium ammonium phosphate was obtained

The solution was made basic. The basicity was checked with the help of pH paperThe solution was filtered and the residue was isolatedThe filtrate was separated into two test tubesSilver nitrate was added to one of the test tubes

White precipitate of Silver chloride was obtained

To the other test tube ammonium chloride and ammonium carbonate was added simultaneously and boiled

White residue of calcium Carbonate was obtained

To the solution left, dilute HCL was added followed by Potassium thiocyanate

Red colour solution marking the presence of Iron was obtained

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Conclusion

Extrapolation from the above observationsConstituents of bone ash identified were:I. CalciumII. PhosphateIII. ChlorideIV. MagnesiumV. IronApart from this Calcium and Phosphate which is found maximum in bone was estimatedfrom the precipitate got. This was done by weighing the precipitate Weight of Calcium carbonate:- 1.7 g Weight of Mg (NH4) PO4 :- 1.1 g Weight of Ca in 2g of sample:- 0.68 g Weight of Phosphorous:- 0.24 g % of Ca:- 34% % of Phosphorous:- 12%

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Precaution

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Bibliography

Ncert chem book

www.icbse.com

Wikipedia

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