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BLASTM 2,OMP-WashU /29-Mar-20031 [decunix5.0a-ev6-1P32LF64 2003-03- 30216:44:251

Query- (1793 letters)

Sequences producing High-scoring Segment Pairs:

Smallest sum

'digti Probability Score P I N ) N

M ' '1 AE011700.1 Xanthomonas axonopodrs pv. c l t . . . 2383 5.3e-387 2 EM~PRO:&~;I~{^~' AEOll699.1 Xanthomonirs axonopodls pv. clt... 1898 6.8e-78 1

>EPq_PRO:AE011700 AE03.l'f00.1 Xanthamonas axanopwdis pv. citri str. 306, s e c t i o n 78 of 469 o f the complete genome.

Length - 9955

Plus Strand RSPs:

Score = 2383 ( 3 6 3 . 6 bits), Expect = 5 . 3 ~ - 2 8 1 , Sum P ( 2 1 = 5.3e-287 Identities = 589/704 ( 8 4 % ) , Positives = 589/704 ( 8 4 % ) , Strand - Plus / Plus

Query: 424 GTGCGCTCCCGATATCTTTGCACGCACCGTTGCAGGTCAATGf;CGGCFAGGTCGG 4 8 3 i l I l l l I l l l l l I l l t l t l l l t l l l l l l l l f I l l l l t l l 1 l l l t l t I l l l l f l l l l l l l

S b j c t : Z GTGCGC'fCCCGATATCTTTWACGCACCG~TGCAEGTCFBTECCGETAAGG 60

Query: 4 8 4 GCTTCCAGAGCGCTATCGAACGCTTTCGAAAGTGACTGTTGACACGATCTTTTAGAGAC 5 4 3 I1 l l l l l l l l l I l l l ~ l i l l l l f l f I t l t l ~ l l l l l l l I ~ l l l l l l i l f l l l l l l l l

S b j c t : 6 3 ACTATCAGAECKTATCGAACGCTTTCGARAGTGACTGTTGACACGATCTTTTAGAGACC 120

- I l l t l t l l 1 l l l i t l t l l l I l l l l l l l l I I 1 I 1 1 1 1 I 1 I l I 1 I ~ I I 1 l l I l i l l l t i 1 l

Sbjct: 121 ATAAGAATCAACTCCACTCrABTTGGTACTTTCCAGTCAGGGCCGTTGGTCAGCMTGGCA 180

Query: 604 TGCGTGCCCGTCCTEEGACGGACGCGGCATCEGCACCCACGEI;GGGECTTGCGGGG'PGC 663 l l l t l l l l l l l l l l l l l t l i l l l I I l l l f t l t l l I l l l I I I I t l l l l l l l l l l l l l l l l l

Sbjct: 181 TGCGTGCCCGTCCTGGGACFGACGCGGCATCGGCACCCACGGGGGGCTTGCEGGTG 240

- 4

I I I ~ I I I I I I I I I ~ ~ I I I I I I ~ I I I I I ~ I I I ~ I ~ I I I I I ~ I I ~ I I I I I I ~ I I I I ~ I I I I Sbjct : 2 4 1 CGCAEACAACAEATCECCGGCGGTTCCGGACATCCGGAACfEGTGACCTGTGCGCGCGTG 300

Query: 724 GTTGGGTTECAA-CGCCG(;GATCTGTCATCGCTGATCGATCGEARCGCCAGTCTT 7 8 2 l111111~11 I l l l l l l I l l l l l I l l l l l l f l l l l l l l l l l l I l l l I l l l l l l l l l l l

Sbjct: 301 ETTEGGTTGCCAGCGCCGEGATCTGTCATCECT~TCGATCEGAACECCAEC 360

Query: 783 CGTCCRCCCATTCCACTTCAGGATTTCTCATGTCGCCTCTCCCGCGTGCCTCECTGT 842 i i i l l l l i ~ I l l l l l l f l I l I l l l l l l l l t l ~ i l l l I l l l l l l l l l l l l I l t l l l t l l

S b j c t : 361 CGTCCAGCCATTCCACTTCAGGATTTCTCATGTCGCCTCTCC€GCGTGCTCGCTGCGTT 420

Query: 8 4 3 T T T G T C C G C C T C C G T C G C T G T C C C T T G C - W T T G C C T T G C T T C G A 900 l i l l I f 11 I l f t l f l l l l l l l l l l I l l t l I I I l l l I l l l l I ~ l11llll1 1

Sbjct: 421 G T T G T T C G C C C T C G T C G C T G T C C C T T G C C R T T G C G C T G T C T C A 180

Query: 901 ACGC-CAGAC-CACGAC-CRTCGGCGGCGGAT-CGGEGCGAATCTCCCMCTCGATGCAC 956 I l i l t i l i l I I i l l I l l i l l l l t l t l I I I I 1 l 1 l l ! l l l l l I l I l l l l I

S b j c t : 481 ACGCGCAGACA~AGGACGCATCGGf:EGCGGC~~GCGGCECAGATI:TCCCAACTCGTGC-C 539

Query: 957 AIbEGTCACCAECTCCTACCRARhCAE-CCTGI(TC:ACCECGATGGACAACAAGCECGACAR 1015 1 I l l I ! i l I I t I I I1 l i I I I l l l i t I l1!111

Sbjct: 540 A---TCA--AGGTC--ACCAGCTCGTACCRGAAGAGCCTGATC-ACGGCCAT-G-GACBA 589

Query: 1016 CTTECGC-ATGACCGACGGCATCTCTCCCAGGACCATC-G-GCAGGTTTCCTGCCGW 1072 1 1 1 1 I ! / I $ 1 l l i I I I I I l l I I l l 1 1 I I 1 1 I !

Sb j c t : 5 9 0 ~IUTICGCGACGACGTGCG-CATGACCGACGGCATC-TCTGCGCAGGA- -CAT-CGECAAE 6 4 4

Query: 1073 CTCCGCCI;GGGCAACECATCACCCATCCGGTGT-GCARRT 1115 I 1 1 1 I i l 1 1 1 1 l I I I1 I ! I I1 I l l

Sbjct: 645 TTTC-CTECCGM-CATCGCCEAGECGATCCAGCGCATCC 686

Lampiran 1. Hasil analisis sekuen gen yang terlibat patogenisitas X. axortopodis pv. glycires (1 793 nt) rnenggunakan program 8lASTN 2,U

>Ef.iPRO:AE011699 AE011699.1 Xanthomonas axonopodis pv. citri str. 306, section 77 o f 469 of t h e com~lete aenone.

Length - 11,301 Plus Strand HSPs:

Score - 1898 ( 2 9 0 . 8 b i t s ) , Expect = 6.8e-78, P - 6.8e-78 Identities - 424 /460 (92%), Pos i t ives - 424/460 (92%) , Strand = Plus / Plus

- .. I l l l l I i 1 1 t l 1 l t l l I l l l l 1 I I1 I t I l l I I I I

S b j c t : 10846 AATTGCTEGGCTTCCCTEC-TACTCCGGAGCGCEGGTTTTTGCGCAACEAFGAAGGTCGA 10904

Guery: 87 TT-CAG--GAAACAGCTATGACTGGCGACGTGCCACAGCGCCCETCTTCTGCACCAT 143 1 1 l l l t l l l I 1 l 1 I 1 1 l I I l t l l l I l l l I I l l l l l l l l l l l l l t f l I l l l l l l l I

Sbjct : 10905 TTGCACTAGAAAC-GCTATGACTGGCGACGTGCCACAGCGCCCGTCTTCTGCACCATGT 10963

Ouerv: 1 4 4 C G T G C A T G C G T T G C G G C T G G T A T G T T C G T T G C G G C G A T T G C C 203 - .. l l l l l l l l l l l l 1 l l l l l l t l t l I l l l l l l i l l l l l l l I l l l f l t I l l l l l I I l l I l I l l

Sbjct: 10964 CGTGCATGCETTGCGGCTGGTATGTTCGTTGCGECGATTGGATGGCGTGTRGGCTCATC 11023

Query: 204 GTTGACA19CGTTGTCGTCGTGGCGCGGCAAGChGTCATCGCGGCACGGGTTTC 263 I l l f l l I l l l l l t l l l l f l l l l l I l l l l l l l l l l l l l I l l t l l l t i l t l l l i l l l t l t I i

Sbjct: 11024 G T P G A C A A C E T T G T C E T C G T G G C G C E G C A R G C l a . G T C A T C G C G G C A C T C T 11083

Query: 264 AAGTACTGCGATACGCKTTTGGAATACACATERGCACGCTGCGAACIEACE(;TGACGCAG 323 l l l f I I l I l l l l l l l l l l l l l l l l l l l l l i l I l t l l l l l l l l l I l l t l t l i l t l l l l l i

Sbjc t : 11084 ARGTACCGCGATACGCGCTTTGGAATACACATEAGCACGCTGCGAAGGACGGTGACGCAG 11143

Query: 324 CCAGCRGTGATTGCGACGTCGGGGCCGCGACGTEGACTGCCWGAATTGETCTEGT~CA 383 I t l t l t l l l I I l l l l t l l l l l l l l l l l l i l l I l l l l l l l l l I t l l l t l t l l l i l l l l

Sbjct: 11144 CCRGCAGTG-T-GCGACGTCGGGGCCGCGA.CGTCGACTGC:CCAGAATTGGTCTEGTCACh 11201

Query: 384 A G G h A T T G G T C A G T M C T G G T C A T G T G A C A A C G R T G C C C A E T G C G C T T T T 443 l l l l t l l l l l l l l t l I l l l t l l l l I l l l l l l l ! i l l l l I t t l I l l t l l I t l l l I I l l l I l

Sbjct: 12202 AGGAATTGGTCAGTGACTGGTCATGTGACFCACGATGCCCAGTGCGCTCCCGATATCTTTG 11261.

Query: 4 4 4 CACGCACCGTTGCAGGTCAATEGCGGCRAGGATCGGGTGC 483 l i l l l l I l I l I ~ l l l I l t i I l l l l I l I l l l l t l l l l t l i

Sbjct : 11262 CACGCACCGTTGCAGGTCAATGCGGTARGEATCGGT 11301

BLASTW 2.0MP-BashU 329-Mar-20033 fdecunix5.0a-ev6-IP3214F64 2003-03-30T16:4$:25]

Query- ( 5 0 0 letters)

Sequences producing High-scoring Segment Pairs:

Smallest Sum

Sigh Probabi l . i t y Score P(NJ W

EM-PRO: +C?-;-r1::I AE011700,I Xanthumonas axonopodis pv. clt. . . 1387 5.2e-75 2

>EM-PRO:Ab'm:i-'m' AE011700.L Xanthomonas axonopodis pv, citri s t x , 3 0 6 , section 7 S of 469 o f t h e complete genome.

Length = 9955

Score - 1387 ( 2 1 4 . 2 bits), Expect = 5.2e-75, Sum ~ ( 2 ) = 5.2e-75 Identities - 307 /323 t 9 5 8 ) , P o s i t i v e s - 307/323 ( 9 5 % ) , Strand = Plus / Plus

Query: 1289 G C C G G T G - C C T E T - G G G C A C G E T A A R - A T C A A - A C - A - C - C A G 1341 I l l l f i l I l l I I l l l l l l l l I l l i l I 1 l l 1 1 l11l l l l l I l l l l l l l l l l l

Sbjct: 885 GCCEGTGECCTATCGEECACGGTCAACATCAACACCACCaBACCCCTGGACTACCAGCAG 9 4 4

Ouerv: 2342 -CCbLAGCTGCTETTCTCAGCCAAFACGChGTACTCCEAGTTTGCCGG-GGTTCGCCFAC 1399 -, ., t l l l l l l l l l l l l l l l l ~ l t l l l l l i l l t l I l l l ~ l t l t l l t l l f 1 t l l l l t l t l t l l

Sbjct : 945 ACCAAECTCrCTGTTCTCAGCCAAAACGCAGThCTCCGRGTTTGCCGGCGGTTCGCCAPICG 1004

Query: 1400 C C C ~ G G G - G T G G C T G A C C T A T A T C G A C C A G T T C B A G T T C G G T G G C G C C T 1458 l l l l l l l t f l t L l l l l l l l l t l i l l f l f I t l l l l l l l l l l l l l l l t l I l l l l l t t l l l

Sbjct: 1005 CCCAAGGGCGTG-CTGACCTATATCEACCAGTTCAAGTTCGGTGCCCTTGCT 1063

Query: 1459 GTTCTCTCBGCGCCGGCTATCAGBAGCTCAAGGATCGCGCCEATTATCCTGTGGATCGAC 1518 l i l i I l l l l l l l l l l l l l l f l I l l l l l l t l l ~ l l l l l l l I 1 l I 1 1 I 1 I t l t l l l l t l l

Sbjct: 1064 GTTC-CTCAGCGCCGGCTATCAEAAGCTCAAEGATCGCGCCEATTATC-TETGGATCGAC 1121

Query: 1519 CGTTGGTACACGCAG-C~CCGACGCCGGChCGTTf;TAThTCCCACGCCGTCCGCGCTAT 1577 l l l l l l l l l l l l l l l I l l t l l l l l l t l i l l l l l l I l l l l ~ l l t l t l l l l l l l I l l l I l l

Sbjct: 1122 C G T T G G T A C A C G C A G G C C A C C G A C G C C E G C A C G T T G T A T A 1181

Query: 1578 CECTCGATCEAGCGCGAGACCAC 1600 I l l i l I l l l l l i l l ~ l l l l I l l I

S b j c t : 1182 CGCTCGATCGAGCGCGAGACCAC 1204

Score = 538 (86.8 bits), Expect = 5.2e-75, Sum P ( 2 ) = 5.2e-75 Identities = 1 $ 2 / 2 4 2 (75&), Positives - 182/242 f 7 5 % ) , Strand - Plus / Plus

Query: 1100 GGTETGCAAATCCCCTCGATCCCCGEGGECGTCCCCATCACTTGCTTGCCCCCCG~ 1160 t l ~ l t l l l 1 1 I t 1 1 1 1 f l l l 1 1 1 I l l I t I 1 1 I l l I I l l1

Sbjct: 687 EGTETGCAGATTTCCACTATCAACGGGCGCGGTTCCACCRTTAGCGTGCGTGGCCTGG 7 4 6

Query: llhl CC-CA-TATCCCCCCCCATCACCGTCAGTGATCAGAAGTGATCARA-G-GCCGATT-C 3215 I1 l l I l l 1 l l 1 l i t 1 I l l 1 l i l I l l l r l l l l I l t ! l l l l I l l

Sbjct: '747 C C G C A G T A T T C G G C C A C - - C A C C A T C W B C G G C C A G - - G T 802

Query : 1216 CGATGE-TTCCGGTAAGA-ATTATC-A-CCAGAAGT-GCCGCCGGCATCGAGGTGATCAA 1270 l l I1 l l l t l l f 1 1 I1 / I I l l I I l l l l l l l l I l l l l l l l l l l l l l l l t l 1111

Sbjct: 803 CGATGGCTTCCGCTRCGACATCATCCAGCCAEAAGTAGCCGCCGGCATCGAEGTGATCAB 8 6 2

Query: 1271 -T-GCCGTCGG-GGACATGGA-GCCGGTE-CCTGT-GGECACGGTELAA-ATCAABCACAR 1323 I I I I I l l1 l l I l I l I l l I l l l l l l I l l I I l l l l l t l l I t I l l i i I I I I

S b j c t : 863 A T C G C C A T C G G C r ; I ; A C A T G G A C G C C ( ; G T E G C C T A T C 923.

Query: 1224 AC 1325 1

S b j c t : 922 CC 923

Lampiran 2. Hasil analisis sekuen gen yang tedibat patogenisitas X. axonupadis pv. glycines (nt 1 i 00-1 600) menggunakan program BLASTN 2.0

AX011700 standard; DNA; PRO; 9955 BP AEOll"100; AE008923;

28-MAY-2002 ( R e l . 71, Created) 06-3uw-2002 ( R e l . 7 2 , t a f t updated, Versi.on 4 )

Xan-nas a x o n w e pv. citr4. la*. 306, secticrr~ 78 of 4 6 9 o f the complete genome.

Xanthomonas axonopodis pv. citri s tr . 306 Bacteria; Proteabacteria; Gamproteobacteria: Xaxlthomonadaceae; Xanthomonas: Xanthomonas axonopodia; Xanthomonas axonopodis pv. citri.

11 1 1-9955 PIEDLIME; ,--L. *

PUBMED; A:,A ;::; ,,':,"( . da Silva A.C.R., Ferro J . A . , Reinach F.C., Farah C . S . , Furlan L.R., Quaggio R . B . , Monteiro-Vitorello C.B., Van Sluys M.A., hlmeida Jr H . P . , ALves L.B.C., do Amasal A.M., Berto l in i Y.C., Camargo L.E.A., Camarotte G., Cannavan F., Cardozo J, , Chambergo F., Ciapina L. P., Cicsrel.li R . M . B. , Coutinho L . L . , Cursino-Santos J.R., El-Doxry El, , Faria J.B., Ferreise A . J . S . , Ferreira R.C,C., Fesro M. I .T. , Formighieri E.F., Franco M.C., Gregglo C.C., Gruber A . , Katsuyama A.M., Kishi L.T., Leite 3r R . P . , Lemos C.F.M., Lemos M.V.F., tocali E.C., Machado M . A . , Madeira A.M.B.N., Martinez-Rossi N . M . , Martins E.C., Meidanis J., Menck C . F . M . , Mlyaki C.Y., Moon D.H., Horeira L . M . , Movo M.T.M., Okura V . K . , Qliveira M.C., 0li"veixa V.R . , Peseira Jr B.A. , Rossi A,, Sena J.A.D., Si3.va C . , de Souza R.F., Spino la L . A . F . , T a k i t a M.A., Tamura R.E., Teixeira E.C., Tezza R. I .D., Trindade dos Santos M., TruEEi D., Tsai S.M., White F . F . , Setubal J . C . , Kitajima J . P . ; "Comparison of t h e genmes o f two Xanthomonas pathogens with d i f f e r i n g host specificities"; Nature 417 (68871 :459 -46J t2002 ) .

1 . ,9955 /db-xref-"raxon: . " /note="pathovar: citri" /organism="Xanthomonas exonopodis pv. c l t r l str. 306'' /stl;ain="306" . > , 390.. 3098 /cotfurl-s t a r t -1 / d b x r e f - " G O A : , . '* /db-xref-"SPTREMRT, : , ,- " /n~ta-~~deneified by sequence sim~lar~ty; putat ive; ORY located using Blastx/Gbimmer/Genemask" /transl-table-ll /gen&="1 TON* /product-"TanB-&psmd=smt rectpptarn

/protein-ld-"

Lampiran 3. Open Reading Frame (URF) gen iroM penyandi TonBdq?endenf receptor gada GenBank (nomor aksesi AEO11700)

SQ Scqucnce 9955 BP; I f 6 3 A; 3131 C; 3217 G : 1844 T; 0 other;

gtgcgcrccc: actatcagag ataagaatca tgcgtgcccg cgcagacaac gttgggttgc cgtccagcca w-togac

garatctttg cacgcaccgt tgcaggtcaa tggcggtaag gatcgggtgc cgctatcgaa cgctttcgaa agtgactgtt gacacgatct tttagagacc actccactga a r t g g t a c t t tccagtcagg gccgttggtc agcaatggca tcctgggacg qacgcggcat cggcacccac gggggggctt gcggggtgct agatcgccgg cggttccgga catccggaac tggtgacctg tgcgcgcgtq cagcgccggg atctgtcatc gctgatcgat cggaacgcca gtcgcgttgt t t ccac t - tca ggatttctca igtugcctct oacgagtgnc togctgqkt ctegtagatg tocctGgcca +tprrgcw* Bctgcttgcc tScgg-2-

M e ~ c gttgaaGqeg m a t t - OogkagqM acEttegutc gclgatgtgcg aqgegagaa ttoctgtako tgctaaggac cggtgcggtg taegqettog aatgttccc ggagctgkcc gcspccagaa gcacggtw camcttcctk gacggacaac trsspctorroa QQ-Wtgg t -wwa tOQCw9PCT cc5rWw-t acaSfwocg gtgctgttt(f cscogcapag wc-sgc skogag-csg acatttcttc ggegtatgag a w e tcgacacqa cataggcagc atgogtckgc g"gg-w ~ m t g c g a tapgaaamka caa&acgct*a cacogatacc tacctgbcta apa-gc cgcoagagao wage-tg tcPgtqgtagq o=w-o goga=*og agt*attacl*a c m t g a c c agcctgaatc tatgoircgaa (gscttgttgc tqcgotttgc ggacgc~aag gkoekggtgc g~ccgaittct ggscag-c &cagcgutcg uktagaagat ttqaccgge acalurtrrc*g g q g ~ ~ ~ c a a awatlrtgto gtcgaccagg gccam-=ww Cctgaaggca ttwagcw cc-ctg g a g w e t : m - c p g oggcgqctg accpkggcag gattctggaa -ca- arreqgo&cct tcmataqcat cgtttquccg gccgccttks aogg-cgt gctgtccagc rarrwaagoag Qagattgtgt gagtagtaw ggagstatot ~ogagatcm cqecact3~cc a a t g a t c a g m seaaggtaas wttw-t *w=ga- wtss8ag- swetggat gccwttga weCSg8- tk-t~ ~ C C ~ C C I I * B ~ ~ g m t t C w C ( * 8 WCgabC-

atttQllapt cagCB#kCct$ tcaqaaawa cEtqgaacgC gacagcgtsc tgggagagcg ogaactactc ggcaogagtS togetasaka atcgaagcga gtaagsscllg g-gcagcg acagcttctt ogegagag&a ggoaaaataa tganqqcaeg anagangatg gatgcama ttg'gctt&coa ggteaaqac slyrctpqtt tocrrgctggg qgcctpl~bt ~t-kacu g-sgguaga a q c g t a w gaa*taacc~ gccsgetQgaa gat$mawc gtamckggta gaagctsctw c e w a w csgtggqmca tqctttqatg ctgaggcagc gcgcttcggg

ID XX AC xx sv XX DT DT XX DE UE XX 0s OC: OC XX fW HP RX RA RA RA RA RA RA RA RA RA RA RA FLA PA m RT KY RL XX FH FH ZT FT FT FT FT FT FT FT FT FT FT FT F'I' FT FT FT FT E'T

AE021699 standard; DNA; PRO; 11301 BP.

28-MAY-2002 (Rel. 71, Created) 28-HAY-2002 ( R e l . 71, Last updated, Version 1)

Xanthomonas axonopodis pv. citri str . 306, section 77 of 470 of the complsre genome.

Xantbomonas axonopcdis pv. citxi str. 306 Bacteria; Protsobacteria; gamma subdivision; Xanthomonas group; Xanthomonas; Xanthomonas axonopodis; Xanthomonas axonopodis pv. citri.

t l l 1-11301 PUBMED; c;3J41:1.1,' . da S i i v a A . C ,R., Ferrn J.A., Reinach F.C., Farah C . S . , Fur lan L.B., Quaggio R . B . , Wvnteiro-Vitorello C . B . , Van S l u y s M.R., Almeida Jr N . F . , Alves L . M . C . , do Amaral A.M., Bertolini M.C., Camargo L.E.A., C a W r O t t e G . , Cannavan F., Cardozo J., Chambergo F., Ciapina L , P , , Cicarelli. R . M . B . , Coutinhu L.L,, Cursino-Sactos S.R., El-Dorry H . , Faria J . B . , Ferreira A . J . S . , Ferreira R.C.C., Fesro M . I . T . , Formighi.eri E.P., Fxanco M.C. , Greggio C . C . , Gruber A., Katsuyama A.M., Kishi L.T., Leite 3r R. P., Lemos E.G.M., Lemos M.V. F., btocali E.C., Machado M.A., Madeira A . M . B . N . , Hartinez-Rossi N . M . , Martins E . C . , Meidanis J., Menck C.I.W., Miyaki C . Y . , Moon D . H . , Mol-eira L.M., Navo M.T.M., Okusa V . R . , Ol ive ira M . C . , Oliveira V . A . , Pereira 3r H.A. , Rossi A . , Sena J. A. D., Silva C . , de Sauza R. F., Spinola L .A, F., T a k ~ . ta M.A., Tamura A.E. , Teixeira E , C . , Tezza P . . f . D . , Tricdade do5 Santos M., T r u f f i . O., Tsai S.M. , White F . F . , Setubal J . C . , Kitaji.ma 3.P.; "Campafisor~ of the genomes o f t w o Xanthomonas pathogens with differing host specificities"; Nature 417 (69871 :459-46312002) .

1. .I1301 /db-xref="taxon: : :: .; - -: " /n~ta-"~athovar:~-c~tri" /oryarlism-"Xanthomonas axonopodis pv, citsi str . 306" /strai.n="306" 10194..18913 /codon,,-s t ar t-1 /note-"identified by sequence similarity; putati.ve; ORF located u s i n g R l . a s t x / G I . imer/Gensrrrark" /transl-table-11 /gene~ '+xc~N*' /product-"type I1 secretion system p r o t e i n h"' / p ro te in id=". ::, , . - * / t ~~~~~~~~.~~="EFTWRWAI.,ILVSMAEVAI,IAPVPLRLVI,PREGLPFSVLDVEGPTW ACiTLRQVQW()G3:VfGDVAVRP~WPLtKGERRVQLQSTQLQ~,~f~LV~~;A(IREMKWAQGef~ LVRQPGGVAII 1 DIaAVQL#VI)LLFDATCrCVQAHGZVSTT~~I,ASGAGALPGLPPLRX~SG12 PACVDATAVLTLLPUTALPAWQAK~QLQIJWPDGRHIIL~'SRVDPA~~UAVLFVFEQT.~LGE' RATPERGELRNDEGRLR"

Larnpiran.4. OpenReadingF=ram~(ORF)genxcsNpenyandifypeIIsecrefion system protein N pada GenBenk (namor aksesi) AEUI f 699

XX SQ Sequence 11301 BP; 1801 A; 3562 C ; 3954 G; I984 T; 0 other;

gcacgcgagc agqgccaact ccgcgtacgc tgcgggtttg cggaatcggg accgtgacac gcatgcgctg ggccttgatc ttggtgtcga tggcgggcgt cgcgctgctg gcattrgfcc cgttgcgtct g#tgc:tgccg cgcgaggggc tgccgttctc ggtqctggat gtggagggcc cgatctgggc cggcacgttg cggcaggtgc aatqgcaaqg catcgtgrtc ggcgatgtgg cggtgcggcc gcaggtctgg ccgctgttgc gtggcgaacg tcgggtgcag ttgcagtcca cacagttgca actgctgttg gtggacggcg cgcaacgcgg catgcgcrat gcacagggcc aactcctggt gcgccagccg qgtggcgtgg cactgatcga tctggcggtg cagctgcagc aggtcgatct gctattcgnt gccaccggct gcgtgcaggc gcacgggcgt gtcagcctgc eattgctggc arrgcggggcg ggcgcgctgc ccggtctgcc gCCaCtgCgC ttgtccgggc agccggcgtg cgtggatcac accgccqtgc tgaccttgct gcccgacacc gcattgcccg ccqgcgtgca qgccaslggcg cagctgcaac tctggcccga cggacgctgg cnactgcagt cgcgcgtgga tccagcgcag gacgcagtgt tgggtgtcgg ac-ttg otgggatw ciqetactcc gpagop~ggg tttttgagen zmpatq- togattgcac tagaaacgct atgactggcg acgtgccaca gcgcccgtct tctgcaccat gatcgtgcat gcgttgcggc tggtatgttc gttgcggcga ttggatggcg tgtaggctca tccgttgaca acgttgtcgt cgtggcgcgg caagcagtca tcgcggcacg ggttcgcctg tgcraagtacc gcgatecgcg ctttggaata cacatgagca cgctgcgaag gacggtgacg cagccagcag tgtgcgacgt cggggccgcg acgtcgactg cccagaattg gtctggtcac aagcqaattgg tcagtgactg gtcatgtgac aacgatgccc agtgcgctcc cgatatcttr gcacgcaccg ttgcaggtca atqwggtsa ggatcgwtg c

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