vesicular traffic ii. endocytic and secretory pathways red = secretory green = endocytic blue =...
Post on 21-Dec-2015
252 Views
Preview:
TRANSCRIPT
Vesicular Traffic II
Endocytic and secretory pathways
red = secretorygreen = endocyticblue = recycling
Different coats are used for different transport steps in the cell
Assembly and disassembly of clathrin coat
Dynamin pinches clathrin coated vesicles from the membrane
Electron micrograph from Drosophila mutant
Resting Chemical Synapse
Drosophila mutant can not recycle synaptic vesicles
SNARE proteins guide vesicular transport
Active Chemical Synapse
Interaction of SNARE proteins while docking synaptic vesicle at nerve terminal
Model for membrane fusion. Tight SNARE pairing forces water molecules from areabetween lipid bilayers
Entry of HIV into lymphocytes
Recruitment of cargo molecules into ER transport vesicles
Vesicular tubular clusters move along microtubules to carry proteins from ER to Golgi
apparatus
Vesicular tubular clusters
Microtubules
Microtubules
DVD Clip 58
3-dimensional model of the Golgi Apparatus
Golgi apparatus in an algal cell
ER – Golgi transitional zone in an animal cell
Some enzymes are enriched in cis or trans compartments of the Golgi
substrates specificfor certain enzymes such as acid phosphatase
Functional Compartmentalization of the Golgi Apparatus
Golgi apparatus stained with GFP
Golgi apparatus can be polarly distributedIn this fibroblast Golgi is facing the direction in which
the cell is crawling
Drawing of goblet cell in the intestinal epithelium
Secretes polysacchariderich mucus into thesmall intestine
Golgi is highly polarizedto facilitate release ofmucus throughexocytosis at theapical domain
N-linked glycosylationbecause sugar is addedto N of asparagine.
original precursor oligosaccharide addedto most proteins in the ER
Oligosaccharide chains are processed in the Golgi
common core
complex
high-mannose
Vesicular transport model
Cisternal Maturation Model
Lysosome interior is different from cytosol
Detection of acid phosphatase in lysosomesSmall spheres may be vesicles delivering the
enzyme from the Golgi Apparatus
top related