vavilov seminar 18 4-2002 genebank - ipk gatersleben

Genetic Resources of Barley in EuropeVavilov - Seminar 18. April 2002

Genebank, IPK Gatersleben

Dirk Enneking

Evaluation and Conservation of Barley Genetic Resources in Europe

Evaluation and Conservation of Barley Genetic Resources in Europe

•Multiplication/characterisation•Resistance

bioticabiotic

•Documentation

•Rationalising the collections

Evaluation and Conservation of Barley Genetic Resources in Europe

•Find new major genes

•Components for horizontal resistance

Evaluation and Conservation of Barley Genetic Resources in Europe

Rhynchosporium secalis (scald)

1 3 5 10 20 30 50 70

% leaf area infected

Evaluation and Conservation of Barley Genetic Resources in Europe

0 10 20 30 40 50 60 70 80 90

100 % attack

scoring date 1. 7. 6. 5. 4. 3. 2. 8.

A 1 A 2 A 3 A 4 A 5 A 6 A 7

Detecting HORIZONTAL RESISTANCE genes

Measuring rate of infection

leaf rust - Puccinia hordei

7 10 14

37

91

179 21

0

206

19

1 2 3 4 5 6 7 8 90

50

100

150

200

250

0 0

28

71

105

88

7

0 0

1 2 3 4 5 6 7 8 90

20

40

60

80

100

120

leafrust

0 7 1042

108

195

264217 203

1 2 3 4 5 6 7 8 9

050

100150200250300

BCC

0 0

6 50

4

14

23 22

1 2 3 4 5 6 7 8 9

05

1015202530

ICA

powdery mildew - Blumeria graminis

6 13 18

64

209 219

226

127

29

1 2 3 4 5 6 7 8 90

50

100

150

200

250

0 1

37

146

86

28

1 0 0

1 2 3 4 5 6 7 8 90

20406080

100120140160

0 6 1347

192247

208 216

124

1 2 3 4 5 6 7 8 9

050

100150200250300

frequ

ency

BCC

04

04

12 1317

25

12

1 2 3 4 5 6 7 8 9

0

5

10

15

20

25

30

frequ

ency

ICA

mildew

netblotch - Pyrenophora teres0 6

361

458

64

8 1 0 0

1 2 3 4 5 6 7 8 90

100

200

300

400

500

0

137

95

59

6 0 0 1 0

1 2 3 4 5 6 7 8 90

20406080

100120140160

scald - Rhynchosporium secalis

1 4

36

270

218

148

60

16 0

1 2 3 4 5 6 7 8 90

50

100

150

200

250

300

0 4

16

39

111

107

21

0 0

1 2 3 4 5 6 7 8 90

20406080

100120140

scald

0 0 452

309 331

251

8116

1 2 3 4 5 6 7 8 9

050

100150200250300350400

BCC

0 0

7

4

11

1 10 0

1 2 3 4 5 6 7 8 9

02468

1012

HOR

barley viruses12

5

3 9

0

20

0

15

2

83

1 2 3 4 5 6 7 8 90

20

40

60

80

100

120

140

Barley Yellow Dwarf Virus- BYDV

0 4 9

25

70 71

83

28

9

1 2 3 4 5 6 7 8 90

20

40

60

80

100

Barley Mild Mosaic- /Barley Yellow-Mosaic VirusBaMMV+ BaYMV/1+ BaYMV/2

2002 Spring barley nurseries for disease testingDISEASE Nursery BCC HOR IG BGRC CFH TotalAphids retest 38 38BYDV retest 1 12 13Leafrust elite 6 1 7

retest 20 7 8 3 38Standard 3 8

Mildew elite 5 2 7retest 6 1 6 1 14standard 2 7

Netblotch elite 16 9 25retest 3 3Standard 10test 14 15

Scald elite 6 3 9retest 1 31 2 7 1 43standard 3

OP_ABK NAME Mildew COSTmildewLeafrust COSTLeafrustScald COSTScaldNetblotchCOSTNetblotch Desired Seed amountMin Seed RequirementACK Dr. Claus Einfeldt x x x x x x 20g 25 seedsLP Dr. Burkhard Schinkel x x xStreng Drs. Stefan Streng/Peter Greif x x x x x 10g 50 seeds (for 2 rows)BAZ-IER_KO Dr. Doris Kopahnke x x 100INRA M. Louis Jestin x x 30 seedsAbed Mr. Morten Helt Poulsen x x x 50 seedsSejet Ole Andersen x x x x x x x 10g 75 seedsSW Mr. Morten Rasmussen x x x x x x x x 20g per nursery 2x25 seedsBoreal Dr. Marja Jalli x x x x x x 10g 25 seedsRISO Dr. Ahmed JahoorPF Dr. Heidi Jaiser x x x x x x 5g 50 seedsNAGREF Dr. Konstandinos BladenopoulosSL Dr. Franz Oeynhausen x x 10g 25 seedsVIR Dr. Irina TerentyevaIHAR Drs. Wieslaw Podyma/Jerzy Czembor x xARI Dr. Andreas G. Kari x x 10g 25 seedsSuceava Dr. Domnica Placinta x 10g 25 seedsKromeriz Dr. Jarmila Milotová x x x x x 20g 25 seedsPiestany Drs. Maria Zakova/Michaela Benkova x 50 seeds, 3 reps

Evaluation and Conservation of Barley Genetic Resources in Europe

Documentation:•European collections

•Linking evaluation data

Evaluation and Conservation of Barley Genetic Resources in Europe

Linking databases-Cultivar documentation-Collecting sites-Taxonomy-Evaluation results

Institut für Pflanzengenetikund Kulturpflanzenforschung

http://barley.ipk-gatersleben.de/ebdb.php3

Institut für Pflanzengenetikund Kulturpflanzenforschung

barley.ipk-gatersleben.de/ebdb.php3

< Update >

Collection Sites

LAT

LON

ALT

Country of origin codes

Co-operators

Institutions

NATIVE Data

International Data

Genotypes

EVA DL AU TA AT ION

Institut für Pflanzengenetikund Kulturpflanzenforschung

Donor numbers= sticky ends

Europe All

No of accessions 102157 137602ACCNAME 66745 92212CULTIVAR REF 22986 27087Most Original No 33882 54404Pedigrees 10880 17706

Europe All

DEU146 15024 18122RUS001 23392 23802USA004 9417 27093USA004_ACCID 9141 26788

Europe All

Seasonal 73341 76272Rainfall 1726Elevation 6274 9393

Kernel cover 14042 + Taxonomy 29464

Row number 15436 + Taxonomy 38492

H. Spontaneum collecting site table: 213 in total

GIS information

Two Gazetteers used-GEOnet Names Server(1)

-Alexandria Digital Library(2)

Resulting in 213 sets of co-ordinates

Plotting and map via DIVA-GIS(3)

1. 164.214.2.59/gns/html/index.html2. fat-albert.alexandria.ucsb.edu:8827/gazetteer/3. gis.cip.cgiar.org/gis/tools/diva.htm

Disease evaluation data

Mapped with Arcview 3.0a

Site No77 1 ISR Menashe Plateau Musmus78 2 ISR Menashe Plateau Tel Megido79 1 ISR Esdraelon Valley Omer80 2 ISR Samarian Mountains Yizrael81 2 PAL Samarian Mountains Mount Gilboa82 4 ISR Eastern Lower Galilee Moledet83 1 ISR Eastern Lower Galilee Afula Ilit84 9 ISR Eastern Lower Galilee Kfar Tavor85 1 ISR Menashe Plateau Yokne'am87 1 ISR Lower Galilee Rehasim89 1 ISR Galilee Coast Lohamei Hagetaot96 1 ISR Lower Galilee Deir Hanna97 3 ISR Lower Galilee Eilaboon99 4 ISR Lower Galilee Segera South100 2 ISR Central Coastal Plain Yavneh101 1 ISR Southern Coastal Plain Kfar Menahem102 3 ISR Judean Foothills Sdot Micha106 1 PAL Judean Foothills Canada

Partner Co-operators FAO Institution1 IPK H. Knüpffer, D.Enneking, M.

Grau, S. Metzing, G. Schütze, R.Selbig, R. Hell

DEU146 Institute of Plant Genetics and Crop Plant Research,Gatersleben

2 ZADI F. Begemann, S. Harrer DEU141 ZADI, Bonn3 GFGF K.-J. Müller DEUGFGF Gesellschaft f. goetheanistische Forschung e.V.,

Neu Darchau4 ACK V. Lein, C. Einfeldt, E. Laubach DEU048 Saatzucht Dr. J. Ackermann, Irlbach5 DSV H. Busch, P. Senft, J. Vaupel, M.

HerrmannDEU011 Deutsche Saatveredelung Lippstadt-Bremen GmbH,

Thüle6 LP B. Schinkel DEU060 Lochow Petkus GmbH, Bergen7 Streng P. Greif, S. Streng DEU069 Saatzuchtgesellschaft Streng's Erben GmbH & Co.

KG, Uffenheim8 BAZ-IER E. Schliephake, D. Kopahnke, A.

Habekuss, G. ProeselerDEU168 Bundesanstalt für Züchtungsforschung an

Kulturpflanzen, Aschersleben9 BAZGB L. Frese, C. Germeier DEU001 Bundesanstalt für Züchtungsforschung an

Kulturpflanzen, Genbank, Braunschweig10 ISC-FIOR M. Stanca, L. Cattivelli ITA024 Istituto Sperimentale per la Cerealicoltura,

Fiorenzuola d'Arda11 ISC-FG N. di Fonzo ITA079 Istituto Sperimentale per la Cerealicoltura, Foggia12 INRA L. Jestin FRA040 INRA –Amelioration des Plantes, Clermont-Ferrand13 GEVES A. Le Blanc SWE002 Groupe d'Etude et de Contrôle des Variétés et

Sémences, Surgères14 NGB M. Veteläinen, M. Hulden, D. T.

EndresenFRA051 Nordic Genebank, Alnarp

15 Abed M. Rasmussen DNK021 Abed Plant Breeding Station, Søllested16 Sejet O. Andersen DNK018 Sejet Plant Breeding, Horsens17 SW R. Jönsson SWE006 Svalöf Weibull AB, Svalöv18 ARC M. Jalli FIN020 Boreal Plant Breeding, Jokioinen19 SLUS R. von Bothmer SWE001 Swedish University of Agricultural Sciences,

Alnarp20 RISO A. Jahoor DNK008 Risø National Laboratory, Roskilde21 PF H. Jaiser, G. Emmert, P.

Geymer, H. HaldrupDNK005 Pajbjergfonden, Odder

22 NAGREF K. Bladenopoulos GRC035 National Agricultural Research Foundation,Thessaloniki

23 CGN L. van Soest, N. Bas NLD037 Plant Research International, CGN, Wageningen24 CRF L. Ayerbe ESP004 INIA Centro de Recursos Fitogenéticos, Alcala de

Henares25 JIC M. Ambrose GBR011 John Innes Institute, Norwich26 SCRI R. Ellis GBR048 Scottish Crop Research Institute, Dundee27 CRX A. Barbier, S. Steyer BEL001 Centre de Recherches Agronomiques, Gembloux28 SL F. Blumenschein, F. Oeynhausen AUT016 Saatbau Linz29 VIR I. Terentyeva RUS001 N.I. Vavilov Institute of Plant Industry30 IHAR J. Czembor, W. Podyma POL001 Plant Breeding and Acclimatization Institute

(IHAR), Centre for Plant Genetic Resources31 Piestany M. Záková SVK001 Research Institute of Plant Production Piestany32 Kromeriz J. Milotová CZE047 Agricultural Research Institute Kromeriz Ltd.33 RICP Z. Stehno, I. Faberová CZE122 Research Institute of Crop Production, Genebank34 ARI A. G. Kari CYP001 Agricultural Research Institute (ARI)35 Suceava D. Placinta ROM007 Genebank Suceava

Partner Co-operators FAO Institution1 IPK H. Knüpffer, D.Enneking, M.

Grau, S. Metzing, G. Schütze, R.Selbig, R. Hell

DEU146 Institute of Plant Genetics and Crop Plant Research,Gatersleben

2 ZADI F. Begemann, S. Harrer DEU141 ZADI, Bonn3 GFGF K.-J. Müller DEUGFGF Gesellschaft f. goetheanistische Forschung e.V.,

Neu Darchau4 ACK V. Lein, C. Einfeldt, E. Laubach DEU048 Saatzucht Dr. J. Ackermann, Irlbach5 DSV H. Busch, P. Senft, J. Vaupel, M.

HerrmannDEU011 Deutsche Saatveredelung Lippstadt-Bremen GmbH,

Thüle6 LP B. Schinkel DEU060 Lochow Petkus GmbH, Bergen7 Streng P. Greif, S. Streng DEU069 Saatzuchtgesellschaft Streng's Erben GmbH & Co.

KG, Uffenheim8 BAZ-IER E. Schliephake, D. Kopahnke, A.

Habekuss, G. ProeselerDEU168 Bundesanstalt für Züchtungsforschung an

Kulturpflanzen, Aschersleben9 BAZGB L. Frese, C. Germeier DEU001 Bundesanstalt für Züchtungsforschung an

Kulturpflanzen, Genbank, Braunschweig10 ISC-FIOR M. Stanca, L. Cattivelli ITA024 Istituto Sperimentale per la Cerealicoltura,

Fiorenzuola d'Arda11 ISC-FG N. di Fonzo ITA079 Istituto Sperimentale per la Cerealicoltura, Foggia12 INRA L. Jestin FRA040 INRA –Amelioration des Plantes, Clermont-Ferrand13 GEVES A. Le Blanc SWE002 Groupe d'Etude et de Contrôle des Variétés et

Sémences, Surgères14 NGB M. Veteläinen, M. Hulden, D. T.

EndresenFRA051 Nordic Genebank, Alnarp

15 Abed M. Rasmussen DNK021 Abed Plant Breeding Station, Søllested16 Sejet O. Andersen DNK018 Sejet Plant Breeding, Horsens17 SW R. Jönsson SWE006 Svalöf Weibull AB, Svalöv18 ARC M. Jalli FIN020 Boreal Plant Breeding, Jokioinen19 SLUS R. von Bothmer SWE001 Swedish University of Agricultural Sciences,

Alnarp20 RISO A. Jahoor DNK008 Risø National Laboratory, Roskilde21 PF H. Jaiser, G. Emmert, P.

Geymer, H. HaldrupDNK005 Pajbjergfonden, Odder

22 NAGREF K. Bladenopoulos GRC035 National Agricultural Research Foundation,Thessaloniki

23 CGN L. van Soest, N. Bas NLD037 Plant Research International, CGN, Wageningen24 CRF L. Ayerbe ESP004 INIA Centro de Recursos Fitogenéticos, Alcala de

Henares25 JIC M. Ambrose GBR011 John Innes Institute, Norwich26 SCRI R. Ellis GBR048 Scottish Crop Research Institute, Dundee27 CRX A. Barbier, S. Steyer BEL001 Centre de Recherches Agronomiques, Gembloux28 SL F. Blumenschein, F. Oeynhausen AUT016 Saatbau Linz29 VIR I. Terentyeva RUS001 N.I. Vavilov Institute of Plant Industry30 IHAR J. Czembor, W. Podyma POL001 Plant Breeding and Acclimatization Institute

(IHAR), Centre for Plant Genetic Resources31 Piestany M. Záková SVK001 Research Institute of Plant Production Piestany32 Kromeriz J. Milotová CZE047 Agricultural Research Institute Kromeriz Ltd.33 RICP Z. Stehno, I. Faberová CZE122 Research Institute of Crop Production, Genebank34 ARI A. G. Kari CYP001 Agricultural Research Institute (ARI)35 Suceava D. Placinta ROM007 Genebank Suceava

Lorenzo Maggione

Jan Konopka, Jan Valkoun, Stefania Grando

Special thanks to:Michael Grau and his team, Gudrun Schütze, Roswitha Selbig at the IPK genebankEdgar Schliephake, BAZ Aschersleben (Biotic stress summaries)Helmut Knuepffer, Genebank, IPK (Project organiser)

Institut für Pflanzengenetikund Kulturpflanzenforschung

Evaluation and Conservation of Barley Genetic Resources in Europe

SEJET PLANT BREEDING

Evaluation and Conservation of Barley Genetic Resources in EuropeEvaluation and Conservation of Barley Genetic Resources in Europe