uv visible instrument types
Post on 12-Jan-2017
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TYPES OF INSTRUMENTS
UV-VISIBLE SPECTROSCOPY
PRESENTED BY THUSHARA MOHAN, M PHARM, GRACE COLLEGE
1.Single beam UV-Visible spectrophotometer
2.Double beam UV-Visible spectrophotometer
SINGLE BEAMSPECTROPHOTOMETER
SINGLE BEAM INSTRUMENT
5
Source of light
Filter/Mono
chromatorsSample
cells
Detectors
Read out device
CONSTRUCTION
WORKING
DISADVANTAGES
1.Fluctuation
2.Rapid scanning is not possible
DOUBLE BEAM SPECTROPHOTOMETER
CONSTRUCTION
Source of light
Filters/Mono
chromatorsSample cell
Reference cell D1
Read out deviceD2
Beam spliter
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