the use of animals in research is a privilege we wish to retain animal welfare versus animal rights...
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The Use of Animals in Research Is a Privilege We Wish to Retain
Animal Welfare Versus Animal Rights
How are Animal Welfare Concerns Reported?
Regulatory Oversight
APHIS1: Animal Welfare Act
NIH: Guide for the Care and Use of Laboratory Animals
AAALAC2: Ongoing Accreditation Inspections
Inspectors Select Animals and their Protocols for Review
Their Review is Based on What is Written
1. Animal and Plant Health Inspection Service (USDA)
2. Association for Assessment and Accreditation of Laboratory Animal Care
Animal Protocols
A Research Facility Must Have an Institutional Animal Care and Use Committee (IACUC)
An IACUC Approved Animal Protocol Is Required Before Any Animal Experiments Can Be Requested Or Begun
New NIH Just In Time Process
Who Can Apply for Animal Protocols at TSRI ?
Research staff (Professors)
Research Associates, with the Professor taking responsibility if an appointment to the research staff is pending on funding
The applicant must have completed all appropriate training
Animal Protocol Review
Protocol Review
Full Committee Review – Monthly
Designated Reviewer – Ongoing
Review Depends on Protocol Content
The Steps Involved
Forms, Instructions, Standard Protocols Booklet in the new IACUC Office in the 3366 Building. A website is coming soon!
The Assurance Declaration to be Signed by the Institutional Official once the Protocol is approved by the IACUC
The Title Page
Boxes to be checked may signal need for full committee review
Collaboration within or out of TSRI (to be explained in the protocol)
1. List the number of animals requested.
2. Provide the experimental plan and use of procedures with scientific justification.
Justify the species and number of animals requested. The use of statistics is recommended by the PHS to determine group size.
Provide information on veterinary care (standard statement)
Protocols involving surgery need veterinary consultation during preparation
Measures to minimize pain and distress including post-operative monitoring and analgesia
Euthanasia
Must conform with the AVMA 2000 Panel Report on Euthanasia or be justified scientifically
Alternatives Statement for Painful or Distressful Procedures:
Include data bases searched (Medline and Agricola a minimum), key words, date of search, must consider the 3 R’s: reduction, refinement, and replacement
Library/resources/animal alternatives web site
No unnecessary duplication must also be assured
General description of the project and its scientific justification
Scientific American style, so lay people on the Committee can understand
Can modify the summary statement from the grant
Antibody production
CFA alternatives statement
Use of Standard Protocols Booklet (see example)
HANDLING (CODE: MH)A mouse should be picked up by grasping its tail close to the body, the loose skin near the nap of the neck or by gently closing your hand around the body. To restrain a mouse for examination and injections, grasp the loose skin at the nape of its neck between your thumb and index finger and place its tail between the fourth and fifth fingers. Be careful not to impede respirations or to grab the tail anywhere but close to the base; remember that the tail can be damaged if excess pressure is applied, or if grasped in areas distal to the base. Mother mice normally move their offspring by picking them up by the nape of the neck. Pain and/or distress level II.
Must provide detailed protocols for any procedures not included in or at variance with the Standard Protocols Booklet
Need to include post procedure monitoring, care, and analgesia
List all substances administered to animals including dose, route, infectious and radioactive compounds
Attach IBC, EH&S, radiation approvals as needed
Biological products, consider contamination
List any work requested outside the animal facility
Required training varies with role in protocol
Training continued
List work done by DAR
Signature of PI taking responsibility for following the protocol
Remember, the animal protocol must cover all animal work requested in the grant
When complete, a copy of the grant must be submitted to be kept on file with the protocol
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