technical note - stitching on biostation im 7-24-12

Note Number: Date: 07/2012 Date Modified: 07/24/2012 Software Version: 2.21 Package: BioStation IM

BioStation IM: Automated Stitch Export Function

This procedure can be used when customers want to

create a “macro” movie by stitching together adjacent

FOVs.

This document describes how to:

Select adjacent FOVs in a batch process to register them as observation positions.

Export adjacent FOVs for automated stitching.

Start-to-Finish Stitching Procedure

There are 4 types of stitching protocols to accommodate the various ways

that a user would configure the FOVs; Types 1 – 4.

Differences between Type 1, Type 2. Type 3, and Type 4:

EXAMPLE #1 - ACQUISITION SETUP AND EXPORT:

Step 1: Click and Hold to create pattern of FOVs in widefield mode.

Step 2: Click radio button above “widefield” option on right-hand side. Then register the

points in a batch format by selecting the large blue arrow as you would normally

register points individually.

Step 3: At completion of the experiment select the “disk” icon to being the export

process.

Step 4: Select the “Export large images multipoint” option

Step 5: Confirm “Type” of acquisition/stitch pattern

Step 6: Review automaticallystitched movie.

EXAMPLE#2 - EXPORTING PROCEDURE:

Step 1: Confirm orientation

(In this example, there are 12 FOVs with FOV#1 in the top left position and

FOV#12 in the bottom right position. This user selected two areas to stitch, upper

FOVs and lower FOVs. Disregard the upper box of FOVs as we are going to

illustrate the protocol using the other set of FOVs.)

Step 2: Select “Export Large Images (Multi points)”

Step 3: Select “Type” of FOV configuration and select channels to include in export.

Confirm that all FOVs were automatically identified and that the orientation is

correct.

Step 4: Select export format (TIFF or AVI) and export location.

(In this example, we chose to export three movies, one for phase, one for

fluorescence, and a combined with phase and fluorescence.)

Step 5: Locate export location and confirm movie through playback.