(table s1)
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(Table S1)
Inoculum PBS
probe antisense sense antisense
1 d 0/1 0/1 0/1
3 d 0/2 0/2 0/2
4d 2/2 0/2 0/2
5 d 5/5 0/3 1/3
6 d 8/8 0/5 1/4
11 d 2/2 ND 0/2
KV
(Figure S1)
D
C
D E
F
E F
anterior
posterior
C
E
A B
Blom
PB
lCa lCa
ON
SOG
lCa
Pe
CC
CC
(Figure S2)
0
20
40
60
80
100
120
0 1 2 3 4 5 6 7 8 9
days
Su
rviv
al r
ate
(%
)
A
B
0 1 4 6 9 14 days
(4) (3) (3) (3) (3) (3)
1.E-04
1.E-03
1.E-02
1.E-01
1.E+00
1.E+01
1.E+02
Re
lativ
e K
aku
go
co
nte
nt
(Figure S3)
C
MB MB
lolo m
CB
ALAL
B
lCa lCa
lolo
m
CB
OES
SOG
m
ON
D
mlo
mlo
mCaCB
OES
SOG
OC
lCamCa
lCa
lCalCa
lo
lo
m
CB
OES
SOG
m
ONA
(Figure. S4)
actin
KV PBS
P NKV
DWV
SBV
ABPV
A
B
actin
DWV
SBV
ABPV
P N
KV + + - -
D
C
1.E-06
1.E-05
1.E-04
1.E-03
1.E-02
1.E-01
1.E+00
(-) (-) (+) (+)
KV
Relative detection level
actin
KV
DWV
SBV
ABPV
P N
PBS (-) (+)F A
(Figure. S5)
10.4 0.5 0.6 0.7 0.8 2 3 4 5 6 7 8
1
0.2
0.3
0.4
0.50.60.7
2
3
4
5
67
KV infected brains
Un
infe
cte
d b
rain
s
GB10708
Kakugo
(Figure S6)
10-4
10-3
10-2
10-1
100
101
days
Rela
tive
expr
essi
on le
vel o
f GB1
0708
0 1 4 6 9 14
(4) (3) (3) (2) (3) (3)N (KV)
N (PBS) (3) (2) (3) (1) (3) (3)
N (NI) (5) (2) (3) (2) (3) (4)
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