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1 Supporting Information
2 3D Quantum Theranosomes: A New Direction for Label-free Theranostics
3 Authors: Sivaprasad Chinnakkannu Vijayakumar a, b, c, Krishnan Venkatakrishnan a, b, c, d, *, Bo Tan c, e
4 aUltrashort laser manufacturing research facility, Department of Mechanical and Industrial Engineering,
5 Ryerson University, 350 Victoria street, Toronto, Ontario, M5B 2K3, Canada
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7 b Institute for Biomedical Engineering, Science and Technology (iBEST), Partnership between Ryerson
8 University and St. Michael’s Hospital, Toronto, Ontario M5B 1W8, Canada
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10 c Nano biointerface Facility, Department of Mechanical and Industrial Engineering, Ryerson University,
11 350 Victoria Street, Toronto, Ontario, Canada, M5B 2K3
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13 d Affiliate Scientist, Keenan Research Center, St. Michael's Hospital, 209 Victoria Street, Toronto, Ontario
14 M5B 1T8, Canada
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16 e Nanocharacterization Laboratory, Department of Aerospace Engineering, Ryerson University, 350
17 Victoria Street, Toronto, Ontario, M5B 2K3, Canada
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19 *Corresponding Author: venkat@ryerson.ca 416-979-5000 Ext: 4984
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Electronic Supplementary Material (ESI) for Nanoscale Horizons.This journal is © The Royal Society of Chemistry 2018
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Primary Theranosome
Secondary Theranosome
1 Figure S1: White Light Emission at Primary and Secondary Theranosomes along with a representative
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1 broadband emission
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NIH3T3 Native
control
Native control
MDAMB-
231
HeLa
Native control
A)
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TheranosomesCell Line
Secondary (Qs- NiO)Primary (Qs-Ni)Non cancerousCancerousNon cancerousCancerous
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% C
ell V
iabi
lity
95% CI for the MeanThe pooled standard deviation is used to calculate the intervals.
Interval Plot of % Cell Viability
2 Figure S2: A) Fluorescence Intensity of NIH3T3, HeLa and MDAMB- 231 cells upon native controls. All 3 the images are set to a standard scale of 10 µm. B) Cell viability of theranosomes is established using 4 model cancerous (MDAMB-231 ) and non-cancerous (NIH3T3) cells
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B)
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24 Figure S3: Stained and unstained control for ROS and Apoptosis assessment
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Unstained control – MDAMB-231 cells
Stained control – MDAMB-231 cells
Unstained control – MDAMB-231 cells
Unstained control –NIH3T3 cells
ROS
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23 Figure S4: SEM and Fluorescence images of NIH3T3, HeLa and MDAMB- 231 cells upon native controls.
24 All the SEM images are set to a standard scale of 10 µm.
HeLa
can
cer c
ells
MDA
MB
cells
NIH
3T3
cells
24 hours 48 hours
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