supplementary figure 1

Supplementary Figure 1

PIG3

cata

lase

MW(kDa)

72

55

43

34

p53

cata

lase

MW(kDa)

7255

43

34

26

95130170

AA BB

-tubulin

catalase

PIG3

VectorPIG3

Cat

alas

e ac

tivi

ty (

U/m

l)

0.0

0.2

0.4

0.6

0.8

1.0

1.2

1.4

H1299 HCT116 p53-/-SW480

AA

DC

F f

luor

esce

nce

leve

l

VectorPIG3

H1299

HCT116 p53-/-

SW480

0

20

40

60

80

100

120

140

160

BB

Supplementary Figure 2

HCT116

siRNA

U2OS RKO

Control p53

p53

p53R2

SESN1

SESN2

GPX1

ALDH4A1

GAPDH

catalase

SOD2

TIGAR

Control p53 Control p53

TP53INP1

Nrf-2

Supplementary Figure 3

catalase

p53

0 4 8 12 24 0 4 8 12 24 (hr)

HCT116 p53-/-

UV (10 J/m2)

-tubulin

HCT116

p53R2

0 0.05 0.1 1 2 0 0.05 0.1 1 2

HCT116 p53-/-

H2O2 (mM)

HCT116

UV (10 J/m2) H2O2 (mM)

0 0.05 0.1 1 2 0 0.05 0.1 1 2

Saos-2 U2OS

catalase

p53

0 4 8 12 24 0 4 8 12 24 (hr)

Saos-2

-tubulin

U2OS

p53R2

Supplementary Figure 4

MW(kDa)

72

55

43ca

tala

se

p53

R2

Supplementary Figure 5

Cat

alas

e ac

tivi

ty (

%)

0

50

100

150

200

250

vector wt R2 R4

n.s.

AA

RO

S le

vels

(%

)

0

20

40

60

80

100

120

vector wt R2 R4

n.s.

Supplementary Figure 6

BB

AABB

HCT116U2OS RKO

p53R2

catalase

-tubulin

Cat

alas

e ac

tivi

ty (

U/m

l)

0.0

0.2

0.4

0.6

0.8

1.0

1.2

1.4

1.6

1.8

n.s.

Control siRNAp53R2 siRNA

HCT116 U2OS RKO0

20

40

60

80

100

120

140

160

n.s.

Control siRNAp53R2 siRNA

Supplementary Figure 7

RO

S le

vels

(%

)

p53R2

catalase

-tubulin

Cat

alas

e ac

tivi

ty (

U/m

l)

HCT116U2OS RKO

AA BB

0.0

0.5

1.0

1.5

2.0

2.5

0

20

40

60

80

100

120

HCT116U2OS RKO

n.s.

n.s.

n.s.

n.s.

n.s.

n.s.

Supplementary Figure 8

Control vectorWild-type p53R2 Mutant p53R2 (V115L)

RO

S le

vels

(%

)

Control vectorWild-type p53R2 Mutant p53R2 (V115L)

p53R2

PIG3

Catalase

p53

β-actin

Sup P

UT

Sup P

PMP70

UV (10 J/m2)

Supplementary Figure 9

UT

catalase merge DAPI

p53

p53R2

p53

p53R2

Supplementary Figure 10

UV (10 J/m2)

0

20

40

60

80

100

120

140

160

180

200

0 8 24 h

Supplementary Figure 11

AA BB

0

5

10

15

20

25

30

35

0 24 48 h

Ap

opto

sis

(%)

UV (40 J/m2)

U2OS (vector)U2OS (catalase)

UV (40 J/m2)

RO

S le

vels

(%

)

U2OS (vector)U2OS (catalase)

0 48 hAd p53

0

20

40

60

80

100

120

140

160

180

H1299 (vector)H1299 (catalase)

Supplementary Figure 12

RO

S le

vels

(%

)

Untreated controlUV (20 J/m2)

HCT116 p53-/-

Vector PIG3

0

20

40

60

80

100

120

140

160

180

200

Supplementary Figure 13

RO

S le

vels

(%

)