superior confi dence in steroid hormone analysis ...workflow manager metidq™ total: 19-40 h for...
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Steroid Hormone Profilingby Mass Spectrometry (LC-MS)
For research use only. Not for use in diagnostic procedures.
Ready-to-Use
Comprehensive Coverage
Best Fit to Physiological Range
SuperiorData Quality
KIT
AbsoluteIDQ® Stero17 Kit
Superior Confi dence in Steroid Hormone Analysis
Steroid hormones are a group of metabolites that are derived from cholesterol. They are produced primarily by adrenal glands, testes, and ovaries. Based on their receptor, steroid hormones are classifi ed into fi ve groups: glucocorticoids, mineralocorticoids, progestogens, androgens, and estrogens. Steroid hormones exert multiple biological functions, including regulation of glucose levels, cell growth, and proliferation. The trend in steroid analysis is moving from measuring single or small sets of steroid hormones with immunoassays towards broader, mass spectrometry-based quantifi cation of steroid hormones (Jeanneret et al., J Chromatogr A 2016).
The AbsoluteIDQ® Stero17 Kit is designed to help researchers to unravel the complexities of steroid hormone effects. It provides highly repro-ducible data on 17 functionally important steroid hormones in a single assay.
Key Benefi tsThe Most Accurate Steroid Hormone Analysis
Best Fit to Physiological Range• Refl ection of actual steroid hormone status• Designed to cover physiological range for the
widest range of conditions
Comprehensive Coverage• Quantifi cation of 17 steroid hormones• All fi ve steroid hormone classes covered
Ready-to-Use• All in one box: no method or reagent
development required• Implemented within one day• For multiple (U)HPLC-MS platforms
High Throughput • Only 7 minutes per sample• Up to 500 samples per week and instrument
Simple, Guided, Automated Workfl ow• Workfl ow manager guided procedure
for enhanced process safety• Automated quantifi cation and technical validation
Superior Data Quality• Quantitative results• Excellent accuracy and precision• Regularly proven in profi ciency tests
Key FeaturesQuantifi cation of 17 Steroid Hormones from all Five Classes
Instrument Platforms*
Metabolite Coverage
Kit Components
• Waters Xevo TQ-S UHPLC • Waters Xevo TQ-S micro UHPLC • SCIEX 5500 HPLC
Gluco corticoids• Cortisol• Cortisone• 11-Deoxycortisol
Progestogens• 17α-Hydroxyprogesterone (17α-OHP)• Progesterone
Mineralo corticoids• Aldosterone• Corticosterone• 11-Deoxycorticosterone (DOC)
Estrogens• Estradiol (E2)• Estrone (E1)
Androgens• Androstenedione• Androsterone• Dehydroepiandrosterone (DHEA)• Dehydroepiandrosterone sulfate (DHEA-S)• Dihydrotestosterone (DHT)• Etiocholanolone• Testosterone
Reagents & Consumables• 96-well SPE plate• Internal & calibration standards• Quality controls• Testmix
Methods & Protocols• Sample preparation protocol• Instrument-specifi c acquisition &
quantifi cation methods• System suitability test
Workfl ow Manager MetIDQ™• Process guidance• Automated technical validation• Basic statistics (optional)
* not suited for nano or micro fl ow systems
KIT
Sample Registration• Register samples• Defi ne plate layout• Generate worklist
Sample Preparation• Perform system suitability test• Prepare kit (loading of samples)
Sample Measurement• Run samples with LC-MS• Instrument-specifi c acquisition
methods are provided
Technical Validation• Perform automated sensitivity and
accuracy check• Inter-plate monitoring of QC results
and precision
Statistics• Perform tests with MetIDQ™ StatPack Module• Use statistical programs of your choice• On demand data analysis by Biocrates
Data Quantifi cation Data Evaluation & Export• Normalize data (optional)• Export results in various fi le formats
Biological Interpretation• HMDB• Consult Biocrates
for support
Workflow Manager MetIDQ™Total: 19-40 h for up to 82 samples
optional
Simple, Guided, Automated Workfl owStandardized and Automated Workfl ows Designed for High Throughput and Robustness
15 min1 h5-6 h
30 min 15 min12 - 32 h(depending on LC-MS system)
The AbsoluteIDQ® Stero17 Kit provides highly robust, quantitative data with excellent accuracy and precision, as well as reproducibility (Koal et al., J Steroid Biochem Mol Biol 2012).
The outstanding kit performance is proven in periodical profi ciency tests organized by the German Society of Clinical Chemistry and Laboratory Medicine (DGKL), demonstrated in the fi gure to the right.
Superior Data QualityProven Accuracy and Reproducibility
Figure: Mean accuracy of 7 steroid hormone levels from quaterly DGKL profi ciency tests (2011-2017) based on 48 samples in total. The orange bars indicate the acceptance range (55%-145%) and the error bars represent ±SD.
A1 2 3 4 5 6 7 8 9 10 11 12
BCDEFGH
Plate LayoutEasy to Use 96-Well Format, for up to 82 Samples
Blank: no sample, no ISTDs
AbsoluteIDQ® Stero17 Kit plate
Zero sample: no sample, only ISTDs
Sample
Internal standards (ISTDs)
7-point calibration standards
Quality control in low, medium, high concentration
Best Fit to Physiological RangeCapture the Broadest Steroid Hormone Profi le
How to Get StartedGlobal Access to High Quality Targeted Metabolomics Results
Your Laboratory Collaborating Laboratory Metabolic Phenotyping Services
You have the right instrumentation to run the kit?
Please contact us for a detailed list of require-ments and a quote.
You want to run the kit in a collaborating laboratory?
Please contact us, and we will help you to fi nd the right partner within a global network of >100 established laboratories.
You want us to measure your samples?
Biocrates can perform the full kit workfl ow, as well as additional services, with short turnaround times. Please contact us for further information.
The serum concentrations of steroid hormones vary considerably depending on age, gender, and health status. The AbsoluteIDQ® Stero17 Kit has been developed to provide highly accurate results that fi t the expected physiological ranges (see table below). For more details, please contact us or refer to the respective client note.
Steroid Hormone
Calibration Standard Calibration RangeCal 1-7 [ng/mL]Cal 1 Cal 2 Cal 3 Cal 4 Cal 5 Cal 6 Cal 7
Gluco-corticoids
Cortisol 1.0 – 1000
Cortisone 0.10 – 100
11-Deoxycortisol 0.010 – 10
Mineralo-corticoids
Aldosterone < 0.025 – 5.0
Corticosterone 0.030 – 30
DOC < 0.0075 – 15
Progestogens17α-OHP 0.050 – 50
Progesterone 0.060 – 9.0
EstrogensEstradiol < 0.005 – 20
Estrone < 0.030 – 15
Androgens
Androstenedione 0.032 – 8.0
Androsterone < 0.050 – 5.0
DHEA 0.12 – 30
DHEA-S 32 – 8000
DHT < 0.012 – 30
Etiocholanolone < 0.060 – 6.0
Testosterone 0.010 – 10
Physiological range in human serum, according to Human Metabolome Database (HMDB), May 2017
Physiological range in human serum, according to Biocrates in-house database, March 2017
< <
Physiological concentration in human serum may be below LLOQ, according to HMDB May 2017 or Biocrates in-house database, March 2017
For research use only. Not for use in diagnostic procedures.For USA and Canada: License pending
Document Nr. 35 029, V02-2019Photos: Biocrates, Fotolia, Depositphotos
Related ProductsAbsoluteIDQ® Stero17 Kit
AbsoluteIDQ® Stero17 Kit HPLC Column
AbsoluteIDQ® Stero17 Kit HPLC Precolumn
AbsoluteIDQ® Stero17 Kit HPLC Setup Box
AbsoluteIDQ® Stero17 Kit UHPLC Column
AbsoluteIDQ® Stero17 Kit UHPLC Precolumn
AbsoluteIDQ® Stero17 Kit UHPLC Setup Box
MetIDQ™ StatPack Module
Data Analysis Service
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