special safety practices for csl2

7/29/2019 Special Safety Practices for CSL2

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Special Safety Practices for CSL21.Work with materials with hazard ratings of NFPA 2 or greater in any category is performed in a properly functioning Class II biological safetycabinet or chemical fume hood. Volatile or flammable materials are used only in a Class II biological safety cabinet or fume hood that is ventedoutside the building. The biological safety cabinets are vented through a thimble connection as in a Class II B3 or through a hard duct as in aClass II B2. The B3 cabinets are used for handling only minute quantities of volatile chemicals with NFPA 3 rating or less. Chemicals with a NFPA4 flammable rating are not used in a biological safety cabinet.2.Appropriate personal protective equipment is specific for the hazard of the chemicals. Safety glasses with side shields are required inlaboratories where chemicals are used or stored. Wearing contact lenses in the laboratory is not recommended, but if an individual must wearthem, they must also wear safety glasses with side shields, chemical splash goggles, or a face shield at all times in the laboratory. Protectivegloves shall be selected on the basis of the chemicals being used. THERE IS NO UNIVERSAL PROTECTIVE GLOVE FOR ALL PURPOSES.FOR THE SAFETY OF PERSONNEL, GLOVES MUST BE CHEMICAL and PURPOSE SPECIFIC.

3.All chemicals must be stored properly, according to compatibility. Any chemicals which pose hazard or risk shall be limited to the minimumquantities required to meet short-term needs of the research program. Chemicals not in use shall be stored in proper storage cabinets and not inchemical fume hoods. Regulated chemicals, such as drugs or radioactive materials, require special secured storage areas. Do not store chemicalson floors.4.A chemical waste label shall identify the contents of each container of chemical wastes and shall be placed in appropriate containers fordisposal through the chemical waste disposal program. Any wastes which pose a special hazard shall be isolated for special handling.

5.Planning shall be guided by these principles:i) be sure that the chemical with its inherent hazards can be used safely in your laboratory, that is, you have the proper facility and availableresources to safely carry out this work;ii) whenever possible substitute less toxic alternatives for more highly hazardous chemicals;iii) use the smallest quantity of chemical that is practical for the experiment or test; andiv) purchase the minimum quantity needed to conduct all experiments.Disposal is not trivial and generally costs 3 to 10 times the original cost of a chemical. This is a hidden cost when you purchase a chemical.

Special Safety Equipment for CSL21.Any refrigerators or freezers used for storage of volatile, flammable chemicals or samples containing volatile, flammable chemicals shall berated as laboratory-safe or explosion-proof. If volatile or flammable chemicals or samples containing volatile, flammable chemicals are not used inthe laboratory, this is not a requirement.2.A vented flammable material storage cabinet, either built in or free- standing, shall be used for the storage of flammable materials. The

cabinets must meet the minimum OSHA standards described in 29 CFR 1910.106.3. Local VentilationNon-volatile toxic chemicals may be used in Class II Type A biological safety cabinet. Volatile or flammable chemicals must be used in a ventedbiological safety cabinet - Class II Type B3 or B2 or a vented chemical fume hood. Class II cabinets are not made for use of large quantities ofchemicals. Class II biological safety cabinets do not have spark- proof electrical systems, and even in the vented B2 or B3 cabinets, volatile andflammable chemicals must be used in such a manner as to prevent the buildup of airborne concentrations that could reach lower explosive limits.Chemicals with NFPA 4 flammable ratings must not be used in Class II biological safety cabinets. Furthermore high concentrations of certainorganic chemicals or corrosive acids or bases may damage the HEPA filter of Class II cabinets and cause the filter to leak. Operations involvingthe use of large amounts of chemicals should be carried out in a chemical fume hood. Further information about biological safety cabinets can befound in Primary Containment for Biohazards: Selection, Installation and Use of Biological Safety Cabinets (CDC/NIH 1995).4.Emergency eye wash stations shall be provided in each laboratory using chemicals and shall be tested weekly by laboratory personnel.Emergency showers shall be within 10 seconds walking distance and shall be tested by the maintenance department. Eye wash stations andshowers must meet ANSI Z358.1 performance standards. The plumbing shall be sized to accommodate the deluge shower and the eye wash

station.5.Carts or safety carriers of rubber or polyethylene for the transport of bottles of acids, bases, solvents, or other hazardous chemicals shall beprovided by each program and used by all personnel in transporting these chemicals.6.Spill control kits shall be available and laboratory personnel shall be trained in their use. Any spills or accidents will be promptly cleaned up.

Special Laboratory Facility Requirements for CSL21.The facility should include a separation of work spaces and desk areas.2.The ventilation system shall be designed such that the room air balance is maintained at a small negative pressure with respect to thecorridors.