sourdough bread

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SOURDOUGH BREAD-A HEALTHY FUNCTIONAL FOOD PRODUCT

Muhammad Qasim Ali Student ID # MFNF13E017

Ph.D. Food science and Technology2nd Semester

INSTITUTE OF FOOD SCIENCE AND NUTRITIONUNIVERSITY OF SARGODHA, SARGODHA

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Functional Foods

“A functional food is similar in appearance to, or may be, a conventional

food that is consumed as part of usual diet, and is demonstrated to have

physiological benefits and/or reduce the risk of chronic disease beyond

basic nutritional functions because they contain bioactive compounds”

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Wheat

Number one food grain consumed directly by mankind

Major source of dietary energy and protein

Staple food of Pakistan. Contributes 68-75 percent of total food intake in daily

diet for the people of Pakistan

(Anjum et al.,1991)

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Fermented Foods Fermented foods provide and preserve vast quantity of nutritious food

Wide diversity of flavors, aroma and texture

Enrich biologically with protein, essential amino acids, essential fatty

acids, and vitamins

Reduce antinutrient content

Decrease cooking time and fuel requirement

Preserve foods between time of harvest and consumption ( Steinkraus, K.H (ed), handbook of indigenous fermented foods, 1983)

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The developing world must rely upon fermentation to preserve and

process its food at costs within the budget of an average consumer

(Steinkraus, 1994)

In recent years sourdough bread has enjoyed renowned success due to

increasing consumer demand for its natural taste and good health effects(Brumner and Lorenz, 1991)

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Fermented foods include

Alcoholic foods/beverages

Vinegar

Pickled vegetables

Sausages

Cheese

Yoghurts

Sourdough breads

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Sourdough

The production of sourdough bread can be tracked back to ancient times

sourdough fermentation is one of the oldest cereal fermentation in mankind

(Spincher, 1999)

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Sourdough A mixture of flour and water

Fermented with lactic acid bacteria (LAB)

Elaborating lactic acid and acetic acid

Resulting in sour taste of end product

(Vyust and Neysens, 2004)

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Sourdough Preparation

Mother sponge

Lactic acid Bacteria- colony-forming units (Cfu) of 108 bacteria per

gram

Yeast -106 (Cfu) per gram

(Devyust et al.,1994)

Sourdough preparation - pH 3.5-4.0

(park et al., 2006)

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Types of Sourdough

Specifically, sourdoughs are grouped into three types, on the basis of the

technology applied i.e. type I, type II and type III sourdough.

The first type sourdoughs are produced in traditional way. To keep the

microorganisms in an active state, these are characterized by

continuous, daily increments.

(Hammes and Ganzle, 1998; Bocker et al., 1995)

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Type II sourdoughs are used as dough-souring supplements during bread

making. These are semi-fluid silo preparations prepared in long fermentation

periods (from 2 - 5 days) and at elevated fermentation temperature often >30˚C

to speed up the activity.

Type III sourdoughs are in dried form, having LAB resistant to the drying

process.

(Bocker et al., 1995)

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Lactic Acid Bacteria in Sourdough• LAB has a long history of use in food and is generally regarded as safe

organisms (Magnusson et al., 2003)2-types of LactobacilliHomofermentative

Producing >85% lactic acid Lactobacillus casei Lactobacillus delbrueckii Lactobacillus fraciminis Lactobacillus plantarum

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Heterofermentative

Producing lactic acid, CO2, Ethanol and Acetic acid in equimolar amount

Lactobacillus brevis

Lactobacillus buchneri

Lactobacillus fermentum

Lactobacillus alimentarius

Lactobacillus amylophillus

(Gobbetti, 1998)

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Yeast in sourdough

The sourdough fermentation is a complex process caused by the combined

effects of the metabolism of yeasts and lactic acid bacteria.

Yeast Responsible for leavening

LAB Responsible for acidification

Typical yeasts associated with LAB in sourdoughs

Saccharomyces exiguus, Candida humilis (formerly described as Candida

milleri), S. cerevisiae and Candida krusei

(Succi et al., 2003)

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Heath Benefits of Sourdough Bread Bakery products, particularly sourdough breads produced from high

extraction rate flours are the best potential source of fiber to reduce

cardio-vascular disease, gastrointestinal disorders and diabetes

(Pomeranz et al., 1977)

Regulation of blood glucose level and decreased risk of diabetes

cardiovascular disease, and certain cancers

(Jacobs et al., 1998)

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Improve Mineral Bioavailability Whole meal cereals are an important source of minerals such as K, P, Mg, or

Zn, but mineral utilization is limited by the presence of phytic acid which is an

anti nutritional factor (Lopez et al., 1998).

The degradation of phytate, has repeatedly been reported in sourdough

processes (Angelis et al., 2003).

Phytate-degrading enzymes exist in cereals, yeast and lactic acid bacteria

isolated from sourdoughs (Lopez et al., 2000).

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Generally, low pH favors degradation of phytic acid and optimal pH value for hydrolysis

is 4.5 in wheat and rye doughs.

Use of sourdoughs or acidified sponges can be adjusted to improve mineral

bioavailability by increasing phytic acid hydrolysis

(Fretzdorff and Brummer, 1992)

Lyophilized cultures of the microorganism may be added to cereal grains such as wheat

to increase the basic nutritive protein quality of the wheat

(El-Megeed et al., 1989)

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Beneficial Effects of Sourdough Fermentation A prolong shelf life through inhibition of mould growth, as Certain sourdough

LAB and their components have antifungal effects against various fungal

species which are isolated from flour and bakery products

(Lavermicocca et al.,

2000)

The application of sourdough to wheat breads has a positive impact on bread

volume, which is a primary quality characteristic of bread

(Hanse and Hansen, 1996)

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Continue…..The application of lactic acid bacteria in the form of sourdough have positive

effect on bread staling. (Armero and Collar, 1998)

The flavor of sourdough wheat bread is richer and more aromatic than wheat

bread, a factor that can be attributed to the long fermentation time of sourdough

(Brummer and Lorenz, 1991)

The sourdough inhibits the growth of the pathogens by synthesizing the

antimicrobial compounds, like lactic acid, acetic acid, benzoic acid and hydrogen

peroxide (Park et al., 2006)

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REFERENCES Armero, E. and Collar, C. 1998. Crumb firming kinetics of wheat bread with anti

staling additives. J. Cereal Sci. 28: 165-174. Brumner, J.M. and Lorenz K. 1991. European Development in Wheat Sourdoughs. Cereal

Foods World. 36: 310-313. Caplice, E. and Fitzgerald, G.F. 1999. Food fermentation: role of microorganisms in food

production and preservation. Int. J. Food Microbiol. 50: 131-149. Cappuccino, J.G. and Sherman, N. 1996. Microbiology: A Laboratory Manual. The Benjamin

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Farooq, Z., Rehman, S. and Bilal, M.Q. 2001. Suitability of wheat varieties/lines for the

production of leavened flat bread (Naan). J. Res. Sci. 12(2) : 171-179.

Anjum, F.M., A. Ali and N.M. Chaudhry, 1991. Fatty acid, mineral composition and functional (bread and chapatti) properties of high protein and high lysine barley line. J. Sci. Food and Agric., 511-519.

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Continue…….. Corsetti, A., Gobbetti, M., Marco, D., Balestrieri, F., Paoletti, F., Russi, L and Russi J.

2000. Combined effect of sourdough lactic acid bacteria and additives on bread firmness

and stailing. J. Agric. Food Chem. 48: 3044-3051.

Corsetti, A., Gobbetti, M., Rossi, J and Damiani P. 1998. Anti mould activity of

sourdough lactic acid bacteria-identification of mixture of organic acids produced by

Lactobacillus sanfrancisco. Appl. Microbiol. Biotechnol. 50: 253-256.

Devyust, E. and Vandamme, E. 1994. Antimicrobial potential of lactic acid bacteria. In

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Continue…….. Frases, P., Lambert, S., Levesque, C., Melicon, D., Richard-Molard, D. and Chiron, H. 1992. The

influence of fermentation on volatile compounds in French bread crumb. Lebsensm.-Wiss. u.-Technol.

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Gobbetti, M. 1998. The sourdough microflora: interaction of lactic acid bacteria and yeasts. Trends in

Food Science & Technology, 9: 267-274.

Hansen, A. and Hansen, B. 1996. Flavor of sourdough wheat bread crumb. Z. Lebensm. Unters. Forsch.

2002: 244-249.

Katina, K., Sauri, M., Alakomi, H.L. and Mattila, S.T. 2002. Potentional of lactic acid bacteria to inhibit

rope spoilage in wheat sourdough bread. Lebensmittel- Wissen schaftund- Technologie, 35(1): 38-45.

Kent, N.L. and Evers, A.D. 1994. Technology of cereals: An Introduction for Students of Food Science

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Continue…..

Laemmli, UK. 1970. Cleavage of structural proteins during the assembly of the head of

bacteriophage. Tu Nature. Pp: 227, 680.

Land, D.G. and Shepherd, R. 1988. Scaling and Ranking Methods. In Piggott JR (Ed.) Sensory

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Lavermicocca, P., Valerio, F., Evidente, A., Lazzaroni, S., Corsetti, A. and Gobbetti, M.

2000.Purification and characterization of novel antifungal compounds from sourdough the

Lactobacillus plantarum 21B. Appl. Environ. Microbiol. 66: 4084-4090.

Lefebvre, D., Gabriel. V., Vayssier, Y. and Fantange-Faucher, C. 2002.Simultaneous HPLC

determination of sugars, organic acids and ethanol in sourdough process. Lebensm.u.-Technol.,

35: 407-414.

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Continue….. Limberjg, H.M.G and Bjorck, I.M.E. 1996. Delayed gastric emptying rate Potentional

mechanisms for lowered glycemia after eating sourdough bread: studies in human and rats

using test products with added organic acids or an organic salts. Am. J. Clin. Nutr. 64: 886-

893.

Lopez, H.W., Duclos, V., Coudray, C., Krespine, V., Feillet-Coudary, C., Messager, A.,

Demigne, C. and Remesy, C. 2003. Making bread with sourdough improves mineral

bioavailability from reconstituted whole wheat flour in rats. Nutrition, 19: 524-530.

Lyford, C.P., Kidd, W., Rayas-Duarte, P and Deyoe, C. 2005. Prediction of flour extraction rate

in hard red winter wheat using the single kernel characterization. Journal of Food Quality, 28:

279-288.

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Continue….. Messen, W., and De, Vuyst. L. 2002. Inhibitory substances produced by lactobacilli isolated

from sourdoughs, a review. Int. J. Food Miccrobiol, 72: 32-43.

Orth, R.A. and Mander, K.C. 1975. Effect of milling yield on lour composition and bread

making quality. Cereal Chemistry, 62(6): 441-446.

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flour from cereal grains of wheat .Plant Food for Human Nutrition, 33(1) : 51-61.

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its relationship to rheological characteristics related to chapatti. Sci. Int. (Lahore) 9(1):55-

59.

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Continue….. Robert, H., Gabriel, V., Lefebvre, D., Rabier, P., Vayssier, Y. and Fontagne-Faucher, C. 2005.

Study of behavior of Lactobacillus plantarum and Leuconostoc starters during a complete wheat

sourdough bread making process. Swiss Society of Food Science and Technology.

Rosenquist, H. and Hansen, A. 1998. The antimicrobial effect of organic acids, sourdough and

nisin against Bacillus subtilus and Bacillus licheniformis isolated from wheat bread. J. Appl.

Microbiol. 85: 621-631.

Spicher, G. and Nierle, W. 1988. Proteolytic activity of sourdough bacteria. Applied Microbial.

Biotechnol. 28:487-492.

Spincher, G. 1999. Zur Geschichte des Suuerteiges. In Spincher G and Stephan H (Eds.)

Handbuch Sauerteig: Biology, Biochemie, Technologie 5th Ed. pp.3-7. Hamburg: Behrs Verlag.

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Continue….. Thiele, C., Ganzle, M.G. and Vogel, R.F. 2002. Contribution of sourdough lactobacilli ,yeast

and cereal enzymes to the generation of amino acids in dough relevant for bread flavor.

Cereal Chem. 79(1): 45-51.

Vetrimani, R., Sudha, M.L. and Rao, P.H. 2005. Effect of extraction rate of wheat flour on the

quality of vermicelli. Food Res. Int. 38: 411-416.

Vuyst, L.D. and Neysens, P. 2004. The sourdough microflora biodiversity and metabolic

interactions. Trends in Food Science & Technology, 16:1-14.

Park, Y.H., L.H. Jung and E.R. Jeon, 2006. Quality characteristics of bread using sourdough.

J. Food.Sci. Nutr., 33: 323-327.

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