single cell isolation

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Single Cell Isolation

Find out how you can isolate the single cell of your choice and get visual confirmation

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QIAscout: single cell isolation

High-density microraft array-based single cell isolation – simple, easy, affordable. You only require an inverted microscope to start working with the device.

Single cell genomics by QIAGEN, 2016

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Components of the QIAscout system

Understand the elegant simplicity of the QIAscout on YouTube

Single cell genomics by QIAGEN, 2016

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Discover QIAscout: single cell isolation

• Access to single cells for every lab

• Number of single cells easily scalable

• Imaging-based cell selection

• Easy to use◦ Choose the cell, release microraft to downstream reaction tube◦ Repeat as often as you want

• Affordable◦ Cost-effective device usable on multiple inverted microscopes present in labs

• Cells stay viable◦ Array can be used as a standard cell culture dish◦ Enables single cell clonal expansion

• Compatible with downstream NGS, WGA, WTA and PCR

Single cell genomics by QIAGEN, 2016

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QIAscout: an easy start to your single cell analysis workflow

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Ideally suited for• Single cell isolation from cell suspensions

• Low volume, precious samples

• Adherent or non-adherent eukaryotic cells from various sample types

• Downstream molecular analyses◦ Aligned with QIAGEN’s single cell analysis portfolio

• Clonal expansion

“QIAscout turned out to be a very suitable system, allowing us to select and transfer our cell of interest with nearly 100% efficiency and survival rate for further downstream applications. The instrument has contributed greatly to our research. ”

Dr. Lajos Kemeny, University of Szeged, Hungary

Single cell genomics by QIAGEN, 2016

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The QIAscout array

• Can be used as standard cell culture dish• Microrafts are magnetic, dislodgeable

and self-sealing• Each array contains 12,000 microrafts

• Cells settle according to Poisson distribution

• 6000 cells equals a 30% chance of having a singe cell in a microraft

Single cell genomics by QIAGEN, 2016

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CellTracker Green

Hoechst

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Image of cells on the QIAscout array

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After isolation cells remain viable and continue to grow

Pierced cluster of HT-29 cells

The same cluster, after several days

of cultivation

Pierced SW48 cell After severll days of cultivation

5 h 24 h 45 h 90 h

MCF-7 cell culture, established after transfer of pierced cell

HT-29 cells after 90 hours

Single cell genomics by QIAGEN, 2016

Read the application note

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Single cell genomics by QIAGEN

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For in-depth, molecular analysis of single cells

Single cell WGA or WTA

Single cell isolation

Single cell sequencing

• Easily access single cells

• Affordable

• Gentle on cells

QIAscoutQIAseq

FX Single Cell Library Kits

REPLI-g Single Cell Kits

Single cell miRNA analysis

• Create superior-quality NGS libraries directly from single cells

• Provides best-in-class amplification of genomes or transcriptomes from single cells

• Profile miRNA expression using qPCR

miScript Single Cell qPCR Kit

Single cell genomics by QIAGEN, 2016

Visit the single cell resource center